MICROBIOLOGICAL RESEARCH
·
2024
·
IF 6.1
Yue-Ying Qin,Yuan Gong,Su-Yun Kong,Zhi-Yuan Wan,Jia-Qi Liu,Ke Xing,Sheng Qin
Plant-associated streptomycetes play important roles in plant growth and development. However, knowledge of volatile-mediated crosstalk between Streptomyces spp . and plants remains limited. In this study, we investigated the impact of volatiles from nine endophytic Streptomyces strains on the growth and development of plants. One versatile strain, Streptomyces setonii WY228, was found to significantly promote the growth of Arabidopsis thaliana and tomato seedlings, confer salt tolerance, and induce early flowering and increased fruit yield following volatile treatment. Analysis of plant growth-promoting traits revealed that S. setonii WY228 could produce indole-3-acetic acid, siderophores, ACC deaminase, fix nitrogen, and solubilize inorganic phosphate. These capabilities were further confirmed through genome sequencing and analysis. Volatilome analysis indicated that the volatile organic compounds emitted from ISP-2 medium predominantly comprised sesquiterpenes and 2-ethyl-5-methylpyrazine. Further investigations showed that 2-ethyl-5-methylpyrazine and sesquiterpenoid volatiles were the primary regulators promoting growth, as confirmed by experiments using the terpene synthesis inhibitor phosphomycin, pure compounds, and comparisons of volatile components. Transcriptome analysis, combined with mutant and inhibitor studies, demonstrated that WY228 volatiles promoted root growth by activating Arabidopsis auxin signaling and polar transport, and enhanced root hair development through ethylene signaling activation. Additionally, it was confirmed that volatiles can stimulate plant abscisic acid signaling and activate the MYB75 transcription factor, thereby promoting anthocyanin synthesis and enhancing plant salt stress tolerance. Our findings suggest that aerial signaling-mediated plant growth promotion and abiotic stress tolerance represent potentially overlooked mechanisms of Streptomyces -plant interactions. This study also provides an exciting strategy for the regulation of plant growth and the improvement of horticultural crop yields within sustainable agricultural practices.
研究领域:
植物学
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微生物学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
·
2022
·Vol. 88
·期 6
·
IF 5.005
Yuan Gong,Jia-Qi Liu,Ming-Jie Xu,Chun-Mei Zhang,Jun Gao,Cheng-Guo Li,Ke Xing,Sheng Qin
Volatile organic compounds (VOCs) produced by microorganisms are considered promising environmental-safety fumigants for controlling postharvest diseases. Ceratocystis fimbriata, the pathogen of black spot disease, seriously affects the quality and yield of sweet potato in the field and postharvest. This study tested the effects of VOCs produced by Streptomyces setonii WY228 on the control of C. fimbriata in vitro and in vivo. The VOCs exhibited strong antifungal activity and significantly inhibited the growth of C. fimbriata. During the 20-day storage, VOC fumigation significantly controlled the occurrence of the pathogen, increased the content of antioxidants and defense-related enzymes and flavonoids, and boosted the starch content so as to maintain the quality of the sweet potatoes. Headspace analysis showed that the volatiles 2-ethyl-5-methylpyrazine and dimethyl disulfide significantly inhibited the mycelial growth and spore germination of C. fimbriata in a dose-dependent manner. Fumigation with 100 μL/L 2-ethyl-5-methylpyrazine completely controlled the pathogen in vivo after 10 days of storage. Transcriptome analysis showed that volatiles mainly downregulated the ribosomal synthesis genes and activated the proteasome system of the pathogen in response to VOC stress, while the genes related to spore development, cell membrane synthesis, mitochondrial function, and hydrolase and toxin synthesis were also downregulated, indicating that WY228-produced VOCs have diverse modes of action for pathogen control. Our study demonstrates that fumigation of sweet potato tuberous roots with S. setonii WY228 or use of formulations based on the VOCs is a promising new strategy to control sweet potato and other food and fruit pathogens during storage and shipment.IMPORTANCE Black spot disease caused by Ceratocystis fimbriata has caused huge economic losses to worldwide sweet potato production. At present, the control of C. fimbriata mainly depends on toxic fungicides, and there is a lack of effective alternative strategies. The research on biological control of sweet potato black spot disease is also very limited. An efficient biocontrol technique against pathogens using microbial volatile organic compounds could be an alternative method to control this disease. Our study revealed the significant biological control effect of volatile organic compounds of Streptomyces setonii WY228 on black spot disease of postharvest sweet potato and the complex antifungal mechanism against C. fimbriata. Our data demonstrated that Streptomyces setonii WY228 and its volatile 2-ethyl-5-methylpyrazine could be a candidate strain and compound for the creation of fumigants and showed the important potential of biotechnology applications in the field of food and agriculture.
研究领域:
微生物学
·
植物病理学
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食品科学
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农业生物技术
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产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2025
·
IF 6.8
Ke Xing,Si Yang,Jing Tao,Sun-Yan Liang,Su-Yun Kong,Sheng Qin
Gray mold disease caused by Botrytis cinerea severely compromises postharvest strawberry quality. As an eco-friendly alternative to chemical fungicides, biological control agents based on microbial volatile organic compounds (VOCs) have emerged as promising candidates. This study developed a VOC-producing fumigant derived from wheat grain-cultured Streptomyces setonii WY228 to control gray mold in postharvest strawberries. Key parameters influencing the fumigant’s antifungal activity, including inoculation volume, culture temperature, incubation time, moisture content and dosage of wheat grains, were systematically optimized to establish a preparation strategy. Storage at 4 °C with vacuum maintained 70 % B. cinerea inhibition with sustained viability of fumigant for 90 d. Application of the fumigant at 26.7 g L −1 suppressed gray mold in postharvest strawberries. The firmness, ascorbic acid content, titratable acidity, and superoxide dismutase activity of fumigated strawberries were 42 %, 54 %, 24 %, and 160 % higher than those of the positive control group, respectively. Four main compounds in VOCs were identified as methyl 2-furoate, 2-methylbutyric acid, 4-methylpentanoic acid, and cyclopentanone, and exhibited antifungal activity and gray mold suppression in strawberries. The wheat grain solid fumigant prepared by S. setonii WY228 demonstrates potential as a biological control agent for gray mold suppression in postharvest strawberries.
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产品关联
DOI
高影响因子
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