Genomic analysis and synergistic effect with cellulase by Streptomyces thermocarboxydus 12219
In this study, we fully sequenced and analyzed the genome of strain 12219 and identified it as Streptomyces thermocarboxydus . The genome contained a single linear chromosome, 6,950,031 bp in size, with a GC content of 72.21 %. This study predicted a total of 6295 genes, including 128 glycoside hydrolase genes, 21 carbohydrate esterase genes, and 54 carbohydrate-binding module genes. When corncob was used as inducer, strain 12219 secreted cellulases and hemicellulases, with xylanase activity reaching 31.15 U/mL. During the hydrolysis of sodium hydroxide treated corn stover, a notable synergistic effect between the 12219 enzyme cocktail and commercial cellulase was observed. And the maximum degree of synergism reached 1.60. When the amount of the 12219 enzyme cocktail added to the commercial cellulase was 5 mg/g, the release of glucose, xylose, and cellobiose increased by 121.35 %, 178.58 %, and 29.33 %, respectively. These findings suggested that the 12219 enzyme cocktail held great potential for industrial applications.
危险品化学品经营许可证（带存储）