Influence of composition changes of Strobilanthes cusia bioactive compounds induced by the hydrolysis and condensation of indican on pathogen biofilm
Strobilanthes cusia is a traditional Chinese medicinal herb containing indole alkaloids, key bioactive components responsible for its pharmacological effects. However, there has been limited research regarding S. cusia compositional impact on antimicrobial properties. This study centered around the hydrolysis and oxidative condensation reactions of indican, aiming to investigate the influence of its intermediate, indoxyl, on pathogenic bacteria growth and biofilm formation. A notable inhibitory effect of indoxyl on Staphylococcus aureus biofilm formation was found. High-performance liquid chromatography and transmission electron microscopy confirmed that indoxyl disrupted the membrane structure of biofilm cells, causing a disturbance in cellular redox balance. These findings suggest that the oxidative reaction of indoxyl within cells could be a prominent factor affecting cell viability. Simultaneously, a portion of indoxyl oxidized externally to form indigo, which did not affect intracellular activities but rather disrupted the biofilm structure. The concept of “compositional changes” enriches the understanding of antimicrobial properties of S. cusia , offering novel approaches for effectively inhibiting pathogenic microorganisms.
危险品化学品经营许可证（带存储）