JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Lijun Yin,Peipei Xu,Junjie Ren,Jing Shi,Runbing Huang,Yunzhen Liu,Yudong Lu,Ruiyun You
Stonehouse clam toxin (STX) is a prevalent neuroparalytic toxin found among shellfish toxins . When consumed by humans, it disrupts neuronal conduction , potentially leading to quadriplegia , headache , shock, and even respiratory failure-induced death. This study introduces a highly sensitive and specific method for detecting shellfish toxins . The approach involves utilizing graphene gold-loaded compounds alongside stone house clam toxin aptamers and their complementary chains. The layered structure of graphene creates numerous hot spots among nanoparticles , while its arched spatial configuration offers an enhanced specific surface area , resulting in improved Surface-Enhanced Raman Scattering (SERS). By combining the signaling molecule Bodipy R6G-modified Apt with GO-Au loading compounds, distinctive Raman characteristic peaks emerge. Due to the strong affinity between Apt and STX, the Apt carrying the signaling molecule migrates away from the SERS aptamer probe's surface, thereby attenuating the original SERS signal intensity and facilitating ultra-sensitive shellfish toxin detection. The detection limit for the toxin was determined to be 5.47 nM, with a recovery rate ranging from 97.60 % to 108.32 %, affirming the applicability of this design for quantitative and specific shellfish toxin detection, signifying its considerable practical significance.
研究领域:
毒理学
·
分析化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Membranes
·
2021
·Vol. 11
·期 12
·951
·
IF 4.106
Xiaoji Zhou,Yizhuo Sun,Shusu Shen,Yan Li,Renbi Bai
In this study, a triple-component copolymer of P(Stx-co-MAAy)-g-fPEGzcontaining hydrophobic (styrene, St), hydrophilic (methacrylic acid, MAA), and oleophobic (perfluoroalkyl polyethylene glycol, fPEG) segments was synthesized and used as an additive polymer to prepare modified PVDF membrane for enhanced anti-fouling performance. Two compositions of St:MAA at 4:1 and 1:1 for the additive and two blending ratios of the additive:PVDF at 1:9 and 3:7 for the modified membranes were specifically examined. The results showed that the presence of the copolymer additive greatly affected the morphology and performance of the modified PVDF membranes. Especially, in a lower ratio of St to MAA (e.g., St:MAA at 1:1 versus 4:1), the additive polymer and therefore the modified PVDF membrane exhibited both better hydrophilic as well as oleophobic surface property. The prepared membrane can achieve a water contact angle at as low as 48.80° and display an underwater oil contact angle at as high as 160°. Adsorption experiments showed that BSA adsorption (in the concentration range of 0.8 to 2 g/L) on the modified PVDF membrane can be reduced by as much as 93%. From the filtration of BSA solution, HA solution, and oil/water emulsion, it was confirmed that the obtained membrane showed excellent resistance to these organic foulants that are often considered challenging in membrane water treatment. The performance displayed slow flux decay during filtration and high flux recovery after simple water cleaning. The developed membrane can therefore have a good potential to be used in such applications as water and wastewater treatment where protein and other organic pollutants (including oils) may cause severe fouling problems to conventional polymeric membranes.Keywords:a triple-component copolymer additive;modified PVDF membrane;hydrophilic and oleophobic surface property;low protein and organic fouling
研究领域:
材料科学
·
环境工程
·
膜技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL SCIENCES
·
2019
·Vol. 35
·期 2
·133-140
·
IF 1.618
Longjie ZHONG,Jiadi SUN,Ying GAN,Shuqi ZHOU,Zijian WAN,Quchao ZOU,Kaiqi SU,Ping WANG
Saxitoxin (STX) is one of the paralytic shellfish poisons (PSP) that endanger people’s health. It is necessary to develop methods for the on-site rapid detection for STX in order to prevent safety accidents. An enzyme-linked immunosorbent assay (ELISA) is timesaving and effective, but it is not suitable for large-scale in-field tests due to the expensiveness of commercial ELISA kits and the bulkiness of a microtiter plate reader (MTPR). In this study, a portable smartphone-based colorimetric analyzer (SBCA) with a cost-effictive enhanced gold nanoparticle-based ELISA (EGNB-ELISA) was proposed for STX detection. In a bicinchoninic acid (BCA) protein assay (R2 = 0.9939) and a glucose assay (R2 = 0.9937), SBCA was shown to be in good agreement with MTPR. EGNB-ELISA had a 12.5-fold lower detection limit (0.4 ng/mL) and a lower detection range (1 – 50 ng/mL, Y = 0.4037X + 0.3564, R2 = 0.9797) than the classical ELISA. The recovery rate ranged over 89.1 – 112.2%. The whole detection system, combining both homemade SBCA and ENGB-ELISA, is expected to satisfy the needs of on-site STX sample tests to guarantee seafood safety.
研究领域:
食品安全
·
分析化学
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
·
2024
·
IF 1
Hu J.,Wu Y.,Kang L.,Liu Y.,Wu Y.,Han D.,Wang J.
Abstract Shiga toxin-producing Escherichia coli (STEC) is an important foodborne pathogen that seriously threats the public and animal health. The major virulence of STEC is Shiga toxin (Stx) which is encoded by Stx prophage. Stx prophage induction leads to the production of Stx. Our recent research has shown that dietary functional sugars can differently regulate Shiga toxin type 2 (Stx2) prophage induction. However, high-throughput assays are required to find more dietary inhibitors for Stx prophage induction. This study aims to establish the recA dual-luciferase reporter system for rapidly screening the functional sugars that can inhibit Stx prophage induction. Results showed that the recA dual-luciferase reporter plasmid was successfully constructed as confirmed by DNA sequencing. Established recA dual-luciferase reporter system showed similar screening results when compared with traditional temperate phage determination method (i.e., qPCR) and suggested that L-arabinose is a dietary functional Stx prophage induction inhibitor. Supplementation with L-arabinose in culture medium reduces Stx production in STEC isolated from fecal samples of diarrhea piglets. Collectively, the recA dual-luciferase reporter system is a promising tool for convenient and rapid screening of dietary Stx prophage induction inhibitor, especially suitable for large-scale screening experiments.
研究领域:
微生物学
·
分子生物学
·
食品安全
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yue Zhao,Yujie Xiao,Xian Yang,Xue Zhang
This study presented the synthesis of phosphorus (P) and chlorine (Cl) co-doped carbon quantum dots (P/Cl-CQDs) derived from Epimedium brevicornu Maxim. and established a simple and effective chemiluminescence (CL) system for detecting Sulfacetamide (STM) and Sulfadimethoxine Sodium Salt (STX). Employing traditional Chinese medicine Epimedium brevicornu Maxim. as a green precursor material, (NH 4 ) 2 HPO 4 and NH 4 Cl were utilized as dopants for P and Cl, respectively. The P/Cl-CQDs was synthesized through a one-step hydrothermal method exhibited excellent CL properties, effectively catalyzing the luminol system and markedly amplifying its CL intensity. Both STM and STX were observed to inhibit the CL intensity of the luminol-(P/Cl-CQDs) system. Based on this, a novel CL method combining flow injection technique was established for the detection of STM and STX. Under optimal conditions, the method demonstrated the capability to detect STM in the range of 1–400 × 10 −6 mol/L, with a limit of detection (LOD) of 0.34 × 10 −6 mol/L and a relative standard deviation (RSD) of 1.29 %, and STX within the range of 5–280 × 10 −6 mol/L, with a LOD of 0.16 × 10 −6 mol/L and an RSD of 1.23 %. This method successfully determined the content of STM and STX in pork, with spike recoveries ranging from 100.0 % to 103.8 % for STM and from 98.0 % to 102.3 % for STX.
研究领域:
分析化学
·
材料科学
·
环境科学
·
药物分析
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）