Regenerative Biomaterials
·
2025
·
IF 8.1
Wu Chunwei,Zhong Jie,Li Jianing,Luo Yande,Wang Junyao,Zeng Xiaodie,Mao Jiaji,Lu Jianping,Xu Junyao,Wu Changqiang,Wang Zhiyong
To address the limitations of low relaxivity and physiological toxicity in commercial gadolinium-based contrast agents for magnetic resonance imaging (MRI), a novel manganese chelate macromolecular system was developed using a flash nano-preparation technique. Herein, the approach applying an instantaneous fluid device incorporated gallic acid, dopamine and Mn2+ to perform in-situ polymerization of dopamine and covalent binding with albumin in a nanoconfined environment. This controllable self-assembly process characterized by its scalability and reproducibility was suitable for industrial-scale production. Under optimized flow rates and material ratios, the synthesized ultrasmall protein-based system, Mn-GA@BSA@DA, exhibited excellent aqueous dispersion with an average size of approximately 18 nm, allowing for long-term lyophilized powder storage. More importantly, the nanosystem demonstrated superior MRI-T 1 relaxivity, significantly surpassing that of clinical Gadopentetate dimeglumine, with a high value around 18.5 mM−1 s−1 and a low r 2/r 1 ratio (< 5 at 3.0 T). Furthermore, this Mn-GA@BSA@DA contrast agent was endowed with tumor-targeting effects and long MRI monitoring window period for the liver, gallbladder and renal tubules. The metal chelation within the nanoagent minimizes Mn2+ release; importantly, the antioxidant components, gallic acid and dopamine, significantly inhibit the Fenton reaction-induced toxicity, enhancing biocompatibility. Therefore, this study presents a simple and scalable production technique for a kind of MRI-T 1 weighted contrast agent with high relaxivity and biocompatibility, offering a promising alternative to commercial Gd chelates.
研究领域:
医学影像学
·
材料科学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Zhongtao Li,Jing Guo,Mengmeng Zhang,Guohua Li,Liguo Hao
Magnetic resonance molecular imaging can provide anatomic, functional and molecular information. However, because of the intrinsically low sensitivity of magnetic resonance imaging (MRI), high-performance MRI contrast agents are required to generate powerful image information for image diagnosis. Herein, we describe a novel T 1 contrast agent with magnetic-imaging properties facilitated by the gadolinium oxide (Gd 2 O 3 ) doping of mesoporous silica nanoparticles (MSN). The size, morphology, composition, MRI relaxivity ( r 1 ), surface area and pore size of these nanoparticles were evaluated following their conjugation with Gd 2 O 3 to produce Gd 2 O 3 @MSN. This unique structure led to a significant enhancement in T 1 contrast with longitudinal relaxivity ( r 1 ) as high as 51.85 ± 1.38 mM −1 s −1 . Gd 2 O 3 @MSN has a larger T 1 relaxivity than commercial gadolinium diethylene triamine pentaacetate (Gd-DTPA), likely due to the geometrical confinement effect of silica nanoparticles. These results suggest that we could successfully prepare a novel high-performance T 1 contrast agent, which may be a potential candidate for in-vivo MRI.
研究领域:
材料科学
·
纳米技术
·
医学影像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2016
·Vol. 16
·期 6
·5569-5576
·
IF 1.338
Li Qinqin,Li Chenglin,Tong Weijun
Here, a novel poly(lactic-co-glycolic acid) (PLGA)-based nanoparticles (NPs) for magnetic resonance (MR) and fluorescence imaging was developed for cell imaging. PLGA NPs loaded with fluorescent dye Nile red (NR) and surface-coated with poly(ethyleneimine) (PEI) were produced in a single
step nanoprecipitation process. Diethylenetriamine pentaacetic dianhydride (DTPA) was conjugated to PLGA/NR@PEI NPs through amidation reaction between –COOH of DTPA and –NH2 of PEI, which can chelate gadolinium (Gd3+) as an MR imaging contrast agent. The PLGA/NR@PEI-DTPA-Gd
NPs exhibited a uniform particle size of ∼200 nm and were stable in culture medium. These NPs had a high T
1 relaxivity (R
1) of 28.36 mM−1S−1. They did not introduce serious cytotoxicity against A549 lung cancer cells.
Furthermore, fluorescence and MR imaging studies on A549 lung cancer cells in vitro revealed that PLGA/NR@PEI-DTPA-Gd NPs can serve as an efficient fluorescence/MR dual-modality imaging nanoprobe.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
医学成像
·
癌症研究
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Gang Shu,Tingting Jiang,Xuening Zhang,Huaixin Zhao
Multimodal imaging is a potent technique that integrates complementary information from various imaging modalities, offering comprehensive insights into disease diagnosis. However, the development of biocompatible multimodal probes remains a challenge. In this study, we present the synthesis, characterization, and application of gadolinium and ytterbium co-doped carbon dots (Yb/Gd-CDs) for triple-modal fluorescence (FL)/magnetic resonance (MR)/computed tomography (CT) imaging. Notably, Yb and Gd were successfully incorporated into the lattice of CDs using a facile one‐pot hydrothermal approach to obtain Yb/Gd-CDs. The Yb/Gd-CDs demonstrated an ultra-small size, excellent colloidal stability, and low toxicity, making them suitable for further in vivo applications. Moreover, the Yb/Gd-CDs exhibited excellent fluorescence, high X-ray attenuation (40.17 HU/g/L), and high T 1 relaxivity (11.16 mM -1 s −1 ) with an ultra-low r 2 /r 1 ratio of 1.08, showcasing remarkable performance in in vivo fluorescence, T 1 -weighted MR, and CT imaging of mice. Yb/Gd-CDs show great promise for in vivo multimodal imaging, and this work provides a highly biocompatible CDs-based theranostic platform with multiple functions for disease diagnosis.
研究领域:
医学影像
·
纳米技术
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Fangnan Xiao,Yue Xiao,Fangman Chen,Xiaolin Liu,Chentao Lin,Jianxin Chen,Yunkun Wu
Highly stable and multifunctional fluorescent quantum dots are particularly attractive in practical applications. Here, a new kind of ultra-small-sized silicon quantum dot-gadolinium (SiQD-Gd) was successfully fabricated by a newly-designed facile hydrothermal growth and chelating method. The obtained SiQD-Gd exhibited outstanding water dispersibility, stability and good fluorescent property with the quantum yield of 11.6%. SiQD-Gd displayed a low cytotoxicity in normal cell lines (HELF, HEK293F) and tumor cell lines (H1299, A549). Meanwhile, SiQD-Gd showed excellent magnetic resonance response with r 1 relaxation rate of 10.5 mmol L −1 ·s −1 and r 2 relaxation rate of 47.5 mmol L −1 ·s −1 , which are 2.5 and 7.4 times enhanced comparing to that of the commercial MR agent Magnevist. In vivo studies showed significant contrast enhancement effect of its T 1 - and T 2 -weighted MR imaging. In addition, in vivo fluorescent imaging for mice and zebrafish indicated its potential applications in fluorescent tracking. Thus, the excellent multimodal imaging capacity and biocompatibility of SiQD-Gd make it a potential imaging agent for clinic applications.
研究领域:
材料科学
·
纳米技术
·
医学成像
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）