Materials Today Communications
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2025
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IF 4.5
Kang Li,Guoge Zhang,Wenfang Li,Shuoxun Jin,Ken Chen,Aihua Yi,Zhongmiao Liao,Wen Zhu,Yi Huang,Zhisheng Zhu,Min Wang,Jiajing Yang,Rujian Liang
The as-deposit (AD) and heat-treated (HT) Al-Cu alloy obtained via wire arc additive manufacturing (WAAM) technology was coated by plasma electrolytic oxidation (PEO), respectively. The reticular big Al 2 Cu grains in the AD Al alloy substrate evolved into numerous small particles and nano precipitates after the heat treatment. The thickness and surface roughness of the PEO coatings on both substrates were increased gradually with the increase of oxidation time. The coating on the HT Al alloy was deposited faster with a rougher surface structure. Although most Al 2 Cu grains were dissolved in the electrolyte at the earlier oxidation stage, some Cu-containing compounds and metallic Cu were gradually deposited in the coatings at the discharge spark oxidation period. The coatings were mainly composed of γ-Al 2 O 3 and α-Al 2 O 3 , and the ratio of α-Al 2 O 3 was increased obviously with the oxidation time. Besides, the coating on the HT sample consisted of a higher ratio of α-Al 2 O 3 than that on the AD alloy, especially at the end of PEO processing. The PEO coatings were made up of two sub-layers, a thin compact inner layer and a porous outer layer. In comparison, the coating on the HT alloy was a bit denser and more uniform. The substrates displayed a adhesion wear trait while the coated samples shew a slight abrasive wear manner over the abrasion test, with a still ball as the friction pair . and the coated HT alloy had a better wear resistance than the coated AD alloy did.
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高影响因子
ACS Omega
·
2025
·
IF 4.3
Mingmin Li,Liang Qi,Jin Huang,Haonan Li,Wei Cheng,Zihan Shi,Xianxing Jiang,Yifeng Zhou,Wanxiang Jiang
Liver fibrosis can progress to cirrhosis and hepatocellular carcinoma. Currently, there is no effective drug for liver fibrosis. The peptide 6 (T6) is an endogenous peptide derived from human intrauterine adhesion tissues and has antifibrotic potential. Here, to improve the long-term efficacy and activity of T6, we conducted the rational modified of T6 through studying structure–activity, and synthesized a series of analogues. Among them, S6 and S6-FA exhibited optimal antihepatic fibrosis activity, and S6-FA had a stronger long-acting effect than T6 and S6. The two analogues inhibited the expression of α-SMA and Collagen 1 in TGF-β-induced LX2 cells model and CCl4-induced mouse model of liver fibrosis. Besides, we discovered that S6 and S6-FA remarkably reduced the AST and ALT serum levels. Mechanistic studies have demonstrated that analogues inhibited liver fibrosis through inhibiting Erk, Smad and P65 pathways. This study provided that the novel peptide S6 and S6-FA is potential candidate compounds for treating liver fibrosis.
研究领域:
医学
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药理学
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DOI
PubMed
高影响因子
Materials Today Communications
·
2021
·
IF 3.383
Yanqing Xue,Bo Li,Xinliang Wang,Xinlei Li,Han Zhang,Qitang Hao
Agglomeration behavior of TiB 2 particles in 5%TiB 2 /Al-4.5%Cu composites (all compositions are in wt.% unless otherwise specified) is studied by adding different content of Mg to improve its strength and ductility, a widely used in-situ molten salt reaction process is used to produce the composites. The results show that addition of Mg could not only significantly segregate TiB 2 particles but also further refine the α-Al grains and then improve the mechanical properties of the composites on the basis of TiB 2 particles. Here, 0.5% Mg is considered the optimum mass fraction for dispersion, refinement, and modification of particles based on the results of investigation. The yield strength (YS), ultimate tensile strength (UTS), elastic Young’s modulus (EM), and Vicker’s hardness (VHN) of the composites in T6 heat treatment are increased to 375 MPa, 486 MPa, 88 GPa, and 167 HV - an improvement of 21.8%, 16.5%, 20.9%, and 9.4%, respectively, versus the original TiB 2 /Al-4.5%Cu composite (308 MPa, 417 MPa, 63 GPa, and 153 HV). The elongation of the composite is improved by 20.9%. These merits are mainly attributed to addition of Mg, which increases the surface-spread coefficient of TiB 2 particles by reducing the surface tension of the Al melt, thereby improving the wettability .
研究领域:
材料科学
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机械工程
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高影响因子
International Journal of Nanomedicine
·
2023
·
IF 8
Aftab Ullah,Kaikai Wang,Pengkai Wu,David Oupicky,Minjie Sun
Background: Liver fibrosis is a chronic liver disease associated with an excessive accumulation of extracellualr matrix (ECM) proteins which ultimately lead to cirrohosis and hepatocellular carcinoma.Purpose: Liver fibrosis therapies that use combination approaches with the ability to affect multiple disease pathways have proven higher efficacies. This study aimed at optimizing and characterizing the co-encapsulation of pirfenidone (PF) and AMD3100 (AMD) into CXCR4-targeted combination liposomes (CTC liposome) for CXCR4 targeting, and the inhibition of major molecular culprits ie α-SMA, CXCR4, TGFβ, and P-p38 involved in liver fibrosis in-vitro.Methods: The CTC liposomes were prepared using the thin-film hydration method. The concentration of encapsulated AMD and PF was measured by HPLC and UV spectrophotometry, respectively. Tramsmission electron microscopy (TEM) was used to determine the liposomal morphology. The CXCR4 targeting ability was determined by CXCR4 redistribution assay. Confocal microscopy and flowcytometry were used to determine the CXCR4 mediated cell uptake. The apoptosis inducing and protein downreguating ability of CTC liposomes were determined by apoptosis assay and western blot analysis, respectively. In-vivo biodistribution and Hoechst staining were used to confirm the feasibility of CTC liposome for the in-vivo applications and drug targeted accumulation, respectively.Results: The TEM studies revealed that CTC liposomes were spherical in shape. The cumulative release of AMD and PF from CTC liposome was 67% and 84%, respectively, at 48 h. Compared to the free drug counterparts, encapsulated drugs displayed higher cell viability. The CXCR4 redistribution assay confirmed the CXCR4 targeting and antagonistic ability of CTC liposomes. The CTC liposomes were internalized more effectively via caveolae-mediated endocytic pathways. CTC liposomes displayed aggressive apoptosis (87.3%) in TGFβ-induced activated HSC-T6 cells suggesting a propensity to fibrosis regression. Also, CTC liposomes significantly reduced α-SMA (65%), CXCR4 (77%), TGFβ (89%), and P-p38 (66%) expressions, better than free drugs. CTC@IR780 liposomes (CTC liposomes incorporating IR780 dye) were more accumulated in fibrotic livers compared to free IR780, as judged by in-vivo imaging, biodistribution analysis, and Hoechst staining. These findings suggest that this simple and stable CTC liposomal system holds a great promise for the treatment and prevention of liver fibrosis.
研究领域:
药理学
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生物技术
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细胞生物学
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分子生物学
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治疗学
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DOI
PubMed
高影响因子
Materials Today Communications
·
2024
·
IF 3.7
Q. Li,J. Shang
Al 2 O 3 /MoS 2 /CePO 4 composite ceramic layer was in-situ prepared on aluminum alloy by micro-arc oxidation. The results show that the content of (CH 3 CO 3 ) 3 Ce·xH 2 O has a great influence on the microstructure of the ceramic layer. When the addition amount is 7.5 g/L, the surface hardness of the ceramic layer is 961.97 HV 1 , which is 1.8 times that of the unadded and 8.36 times that of the matrix. Tribological tests show that the moderate addition of (CH 3 CO 3 ) 3 Ce·xH 2 O also significantly improves the wear resistance of the ceramic layer. Composite layer research can be developed and applied to other alloys to improve their friction properties.
研究领域:
材料科学
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表面工程
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高影响因子
JOURNAL OF THERMOPLASTIC COMPOSITE MATERIALS
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2026
·
IF 4
Zhenyang Qi,Jianxiang Lin,Shiqiang Yan,Kezhang Zhang,Wenhao Fan,Qiang Zhang,Shaoping Chen
Carbon fiber-reinforced polymer (CFRP) composites are increasingly employed in automotive, aerospace, and related industries to achieve lightweight structural designs. Although friction stir lap welding (FSLW) has been widely utilized for metal-thermoplastic composite joining, most existing studies have focused on static strength and fracture behavior, with limited attention to fatigue performance and dynamic damage evolution. In this work, high-aluminum-content particle-filled PA66 composites were manufactured using an electric-field-activated hot-press sintering process and subsequently joined to 6061-T6 via FSLW. The dissimilar joints consistently failed on the composite side under both static and cyclic loading, exhibiting a static tensile strength of 1935 N. As the composite material governed joint failure, fatigue characterization concentrated on the composite side. At 41.7% of the static strength, the composite achieved a fatigue life of 602,999 cycles. These results demonstrate that the proposed dissimilar FSLW joint possesses promising fatigue resistance, supporting its potential application in lightweight transportation structures. The results also provide a basis for future studies aimed at optimizing interfacial design and enhancing the long-term durability of metal-polymer hybrid joints.
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高影响因子
BIOMEDICINE & PHARMACOTHERAPY
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2018
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IF 3.457
Lifeng Qin,Jinmei Qin,Xiumei Zhen,Qian Yang,Liyi Huang
The C-X-C motif chemokine 12/C-X-C chemokine receptor type 4 (CXCL12/ CXCR4) biological axis plays an important role in the pathogenesis of liver fibrosis . Curcumin is known to have an anti-fibrosis effect, but the specific mechanism needs to be elucidated. There is currently no evidence illustrating a connection between curcumin and the CXCL12/CXCR4 axis in liver fibrosis . Here, we investigated the contribution of curcumin on CXCL12/ CXCR4 biological axis in liver fibrosis. Our results showed that curcumin remarkably improved hepatic function and liver fibrosis, and the effects are similar as silymarin . The alleviation of liver fibrosis with curcumin treatment was associated with a reduction of CXCL12, CXCR4, α-SMA and RhoA. In addition, curcumin markedly inhibited the proliferation and migration of HSC-T6 cells. This study indicates that curcumin could protect against hepatic stellate cells activation and migration by inhibiting the CXCL12/CXCR4 biological axis in liver fibrosis.
研究领域:
药理学
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肝病学
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分子生物学
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Jian Sun,Huayi Qu,Waseem Ali,Yan Chen,Tao Wang,Yonggang Ma,Yan Yuan,Jianhong Gu,Jianchun Bian,Zongping Liu,Hui Zou
Microplastics (MPs) and cadmium (Cd) are important environmental pollutants, that damage the liver. However, the effect and mechanism of combined Cd and MPs exposure on liver fibrosis are still largely unknown. In this study investigated, Cd + MPs exposure increased superoxide anion production and promoted extracellular ATP release compared with exposure to Cd or MPs individually. Cd + MPs increased inflammatory cell infiltration , activated the P2X7-NLRP3 signaling pathway, and promoted inflammatory factor release. Cd + MPs aggravated Cd- or MPs-induced liver fibrosis and induced liver inflammation. In AML12/HSC-T6 cell in vitro poisoning model, exposure of AML12 cells to Cd + MPs increased the opening of connexin hemichannels and promoted extracellular ATP release. Treatment of HSC-T6 cells with the supernatant of AML12 cells exposed to Cd + MPs significantly promoted HSC-T6 cell activation. Treatment of HSC-T6 cells with different concentrations of ATP produced similar results. TAT-Gap19TFA, an inhibitor of connexin hemichannels, significantly inhibited the ATP release and activation of Cd + MPs-treated HSC-T6 cells. Finally, the expression of the ATP receptor P2X7 was silenced in HSC-T6 cells, which significantly inhibited their activation. In conclusion, exposure to Cd + MPs promoted liver fibrosis through the ATP-P2X7 pathway and synergistically affected liver inflammation and fibrosis.
研究领域:
环境科学
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毒理学
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细胞生物学
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分子生物学
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DOI
PubMed
高影响因子
WORLD JOURNAL OF GASTROENTEROLOGY
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2020
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IF 3.665
Qing-Hong Du,Chu-Jun Zhang,Wei-Hong Li,Yan Mu,Ya Xu,Scott Lowe,Lin Han,Xue Yu,Shu-Yan Wang,Yu Li,Jian Li
BACKGROUND Liver fibrosis is a common health problem worldwide and there is still a lack of effective medicines. The Chinese herbal medicine, Gan Shen Fu Fang (GSFF) is composed of salvianolic acid B and diammonium glycyrrhizinate. In this study, we observed the effects of GSFF on liver fibrosis in vivo and in vitro in an attempt to provide some hope for the treatment. AIM To observe the effects of GSFF on liver fibrosis in vivo and in vitro and investigate the mechanism from the perspective of the inflammatory response and extracellular signal-regulated kinase (ERK) phosphorylation. METHODS Common bile duct-ligated rats were used for in vivo experiments. Hepatic stellate cells-T6 (HSC-T6) cells were used for in vitro experiments. Hematoxylin and eosin staining and Masson staining, biochemical assays, hydroxyproline (Hyp) assays, enzyme-linked immunoasorbent assay and western blotting were performed to evaluate the degree of liver fibrosis, liver function, the inflammatory response and ERK phosphorylation. The CCK8 assay, immunofluorescence and western blotting were applied to test the effect of GSFF on HSC-T6 cell activation and determine whether GSFF had an effect on ERK phosphorylation in HSC-T6 cells. RESULTS GSFF improved liver function and inhibited liver fibrosis in common bile duct-ligated rats after 3 wk of treatment, as demonstrated by histological changes, hydroxyproline assays and collagen I concentrations. GSFF alleviated inflammatory cell infiltration and reduced the synthesis of pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α) and interlukin-1β] and NF-κB. In addition, GSFF decreased ERK phosphorylation. In vitro , GSFF inhibited the viability of HSC-T6 cells with and without transforming growth factor β1 (TGF-β1) stimulation and decreased the synthesis of collagen I. GSFF had the greatest effect at a concentration of 0.5 μmol/L. GSFF inhibited the expression of α-smooth muscle actin (α-SMA), a marker of HSC activation, in HSC-T6 cells. Consistent with the in vivo results, GSFF also inhibited the phosphorylation of ERK and downregulated the expression of NF-κB. CONCLUSION GSFF inhibited liver fibrosis progression in vivo and HSC-T6 cell activation in vitro . These effects may be related to an alleviated inflammatory response and downregulated ERK phosphorylation.
研究领域:
药理学
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肝病学
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分子生物学
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DOI
PubMed
高影响因子
Chinese Herbal Medicines
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2024
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IF 4.7
Xin Wu,Cuiwei Chen,Hongyan Zhang,Keda Lu,Gang Cao
Objective This study is mainly to elucidate the characteristic components, hepatoprotective effect, and the underlying anti-hepatic fibrosis molecular mechanism of the total flavonoids of Tetrastigma hemsleyanum Diels & Gilg (SYQTF). Methods The components of SYQTF were confirmed based on global natural products social molecular networking (GNPS) as well as the hepatoprotective effect on CCl 4 -induced acute liver injury. Cell toxicity and viability, cell cycle, and apoptosis analyses were then performed to determine the effects of SYQTF in transforming growth factor-β1 (TGF-β1) (10 ng/mL)-induced HSC-T6 hepatic fibrosis cell model. Finally, the lentiviral particles to package recombinant Ras-related C3 botulinum toxin substrate 1 (Rac1) over-expression and Rac1 knockdown plasmids were transfected into TGF-β1-induced HSC-T6 cells to elucidate the mechanism of anti-hepatic fibrosis of SYQTF targeting Rac1, respectively. Results Our findings demonstrated that 86 components in SYQTF were identified in MS E and FastDDA mode with the aid of GNPS. Moreover, S 15 -ZJ, the genuine functional food and medicinal materials of T. hemsleyanum , was significantly different from other regions by multivariate statistical methods based on progenesis QI. Remarkably, kaempferol-3- O -furananose7- O -rhamnosyl-glucoside, the highest response intensity in S 15 -ZJ, may act as the potential Q-marker of genuine functional food and medicinal materials of T. hemsleyanum in Zhejiang Province distinguished from other areas. In addition, the in vivo experiments revealed that SYQTF greatly alleviated CCl 4 -induced acute liver injury. Furthermore, SYQTF can reversible the activation and proliferation of HSC-T6 cells, block the cell cycle in the G 0 /G 1 phase, and promote cell apoptosis. Remarkably, the study is the first to reveal that SYQTF exhibited the anti-hepatic fibrosis effect through targeting Rac1 in the hedgehog signaling pathway. Conclusion Rac1 is an important signaling molecule for the activation of the hedgehog pathway in HSC-T6. Importantly, SYQTF exerted its anti-hepatic fibrosis effect by inhibiting the Rac1-hedgehog signaling pathway. Furthermore, kaempferol-3- O -furananose7- O -rhamnosyl-glucoside could be a potential Q-marker of T. hemsleyanum in Zhejiang Province distinguished from other areas.
研究领域:
药理学
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分子生物学
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肝病学
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天然产物化学
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DOI
高影响因子
MACROMOLECULAR BIOSCIENCE
·
2017
·Vol. 17
·期 9
·1700143
·
IF 3.238
Shan Yan,Lin-Yan Wan,Xiao-Jie Ju,Jiang-Feng Wu,Lei Zhang,Ming Li,Zhuang Liu,Wei Wang,Rui Xie,Liang-Yin Chu
In this work, a novel type of block copolymer micelles with K + -responsive characteristics for targeted intracellular drug delivery is developed. The proposed smart micelles are prepared by self-assembly of poly(ethylene glycol)- b -poly( N -isopropylacry-lamide- co -benzo-18-crown-6-acrylamide) (PEG- b -P(NIPAM- co -B18C6Am)) block copolymers. Prednisolone acetate (PA) is successfully loaded into the micelles as the model drug, with loading content of 4.7 wt%. The PA-loaded micelles display a significantly boosted drug release in simulated intracellular fluid with a high K + concentration of 150 × 10 −3 m , as compared with that in simulated extracellular fluid. Moreover, the in vitro cell experiments indicate that the fluorescent molecules encapsulated in the micelles can be delivered and specifically released inside the HSC-T6 and HepG2 cells responding to the increase of K + concentration in intracellular compartments, which confirms the successful endocytosis and efficient K + -induced intracellular release. Such K + -responsive block copolymer micelles are highly potential as new-generation of smart nanocarriers for targeted intracellular delivery of drugs.
研究领域:
药学
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纳米技术
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药物递送系统
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细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2022
·
IF 5.195
Min Hao,Zhouhui Yao,Mengting Zhao,Ziyan Chen,Pingping Wang,Xianan Sang,Qiao Yang,Kuilong Wang,Xin Han,Gang Cao
Ethnopharmacological relevance Curcumae Rhizoma-Sparganii Rhizoma (CR-SR) is a classic herbal pair to promote blood circulation and remove blood stasis in ancient China. However, the molecular mechanism is still unclear. Aim of study To screen out the anti-liver fibrosis active ingredients in CR-SR. Moreover, preliminary exploration the molecular mechanism of CR-SR to ameliorates liver fibrosis . Materials and methods In this research, plant taxonomy has been confirmed in the “The Plant List” database ( www.theplantlist.org ). The chemical components of CR-SR were analysed by ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-Q/TOF-MS). “Component-Target-Pathway-Disease” network of CR-SR components were built by network pharmacology . Then, the interaction between primary components and predicted protein targets based on network pharmacology were validated by molecular docking. The pharmacological actions of CR-SR were verified by blood biochemical indexes, histopathologic examination of CCL 4 induced rats’ model. The core protein targets were verified by Western blot . The effects of screened active components by molecular autodocking were verified by HSC-T6 cell experiment. Results The result shows that 57 chemical constituents in CR-SR herbal pair were identified by UPLC-Q/TOF-MS, in which, 27 compounds were closely connected with liver fibrosis related protein targets. 55 protein targets screened out by “component-target-pathway-disease network” maybe the underlying targets for CR-SR to cure liver fibrosis. Moreover, the 55 protein targets are mainly related to RNA transcription, apoptosis , and signal transduction. The molecular autodocking predicted that ten components can bond well with PTGS2 and RELA protein targets. The blood biochemical indexes, histopathologic examination of CCL 4 induced rats experiment showed that CR-SR has well intervention effect of liver fibrosis. The Western blot analysis indicated that CR-SR could significantly inhibit RELA, PTGS2, IL-6, SRC, and AKT1 protein expression to exert the anti-fibrosis effect. The HSC-T6 cell experiment indicated that both formononetin (FNT) and curdione could significantly inhibit the activation of HSC and reduce the expression of PTGS2, and p-AKT1 which was accordance with the molecular autodocking results. Conclusion This study proved the molecular mechanism of CR-SR multi-component and multi-target anti-liver fibrosis effect through mass spectrometry, network pharmacology, and western blotting technology. The research provides a theoretical evidence for the development and utilization of CR-SR herbal pair.
研究领域:
药理学
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分子生物学
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网络药理学
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中药学
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肝病
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）