Xiang Han,Lei Zhao,Dong-En Wang,Juan Xu,Longlong Zhang,Mao-Sen Yuan,Qin Tu,Jinyi Wang
In this paper, we designed and synthesized a novel TBET-based ratiometric fluorescent chemodosimeter, RH-Au, for Au3+. It was found that the probe RH-Au displayed highly selective, sensitive and naked-eye detection upon the addition of Au3+. The probe RH-Au can be used in the pH range 6.0–7.5 and the detection limit was determined to be as low as 2.91 nM (0.57 ppb). We also demonstrated a successful application of imaging Au3+ in living cells using RH-Au.
A naphthalimide-rhodamine fluorescent turn-on probe RHSNO with red emission for hypochlorous acid based on dual reaction linkage induce through-bond energy transfer (TBET) system was designed and synthesized. Rhodamine B thiohydrazide and naphthalimide -N,N- dimethylthiocarbamate (DMTC) were linked to form a structure of monothio-bishydrazide. In addition, introduce dimethylthiocarbamoyl chloride as another reaction site of ClO − , and it acts to quench the fluorescence of naphthalimide. In the presence of ClO − , RHSNO exhibits high selectivity toward ClO − at trace levels over other anions/ROS based on ClO − -triggered intramolecular desulfurization cyclization of rhodamine-thiohydrazide, the spiro ring of rhodamine was opened and meanwhile desulfurization hydrogenation happened at DMTC of naphthalimide moiety. These dual linkage reaction happened simultaneously made a typical TBET process took place from naphthalimide donor to rhodamine acceptor, an obvious increase of rhodamine emission at 590 nm and enhanced up to 260-fold gradually after adding ClO − with the increase of the concentrations. The efficiency of energy transfer was calculated to be 86%. And the quantum yield of rhodamine moiety is 0.64. Besides, the chemodosimeter RHSNO for ClO − with high sensitivity, a dramatic color change from colorless to brightly pink among 2 s after the addtion of ClO − . The detection limit was determined to be 22.1 nM and works excellently within a wide pH range of 5–10. Moreover, RHSNO had been employed for endogenous imaging in MCF-7 cell for ClO − . The results indicated that the probe could permeated into the cells and reacted with the intracellular ClO − . It is excellent to design fluorescence sensor RHSNO as a quick and effective method for detecting excess ClO − in living cell, for maintaining the balance of hypochlorite in the body and ensure normal life activities.
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