ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Yafang Wu,Yinglin Wang,Youxue Wu,Zhaoyi Feng,Dezhi Li,Wenjun Zhao,Qing Liu
Background Salmonella, a foodborne pathogen poses significant threats to food safety and human health. Immunochromatographic (ICTS) sensors have gained popularity in the field of food safety due to their convenience, speed, and cost-effectiveness. However, most existing ICTS sensors rely on antibody sandwich structures which are limited by their dependence on high-quality paired antibodies and restricted sensitivity. For the first time, we combined multi-line ICTS strips with fluorescent bacterial probes to develop a label-free multi-line immunochromatographic sensor capable of detecting broad-spectrum Salmonella . Salmonella was labeled with the aggregation-induced luminescence material TCBPE, resulting in its transformation into a green fluorescent probe. Results Using this sensor, we successfully detected Salmonella typhimurium within the concentration range of 10 4 –10 8 CFU/mL with a visual detection limit of 6.0 × 10 4 CFU/mL. Compared to single-line sensors, our multi-line sensor exhibited significantly improved fluorescence intensity resulting in enhanced detection sensitivity by 50 %. Furthermore, our developed multi-line ICTS sensor demonstrated successful detection of 18 different strains of Salmonella without any cross-reaction observed with 5 common foodborne pathogens tested. The applicability and reliability were validated using milk samples, cabbage juice samples as well and drinking water samples suggesting its potential for rapid and accurate detection of Salmonella in real-world scenarios across both the food industry and clinical settings. Significance In this experiment, we developed a TCBPE-based multiline immunochromatographic sensor. Specifically, Salmonella was labeled with the aggregation-induced luminescence material TCBPE, resulting in its transformation into a green fluorescent probe. Through the multi-line analysis system, the detection sensitivity and accuracy of the sensor are improved. In brief, the sensor does not require complex antibody labeling and paired antibodies, and only one antibody is needed to complete the detection process.
研究领域:
食品安全
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微生物学
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生物传感
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Chentao Li,Yafang Wu,Haijuan Zeng,Yinglin Wang,Ying Yu,Jinlong Jiao,Dezhi Li,Qing Liu
The presence of Acidovorax citrulli (Ac), the etiological agent responsible for bacterial fruit blotch (BFB), exerts a profound impact on the growth and development of cucurbit plants. Therefore, it is imperative to promptly detect Ac to effectively mitigate the widespread dissemination of this disease among cucurbit crops. The present study describes the development of a novel label-free immunochromatographic strip (ICTS) sensor for the detection of Ac in cucurbit samples. This was achieved by utilizing 4’,4’’’,4’’’’’,4’’’’’’’-(ethene-1,1,2,2-tetrayl) tetrabiphenyl-4-carboxylic acid (TCBPE) carboxyl activation product (CA-TCBPE) as a fluorescent probe. The CA-TCBPE@Ac complex is captured at the detection site through the immobilization of a monoclonal antibody monoclonal antibody (mAb) targeting Ac in this sensor. Under the excitation of 365 nm ultraviolet (UV) light, the detection of Ac in the concentration range of 10 5 -10 8 CFU/mL was successfully achieved, with a visual detection limit of 10 5 CFU/mL. The accuracy of the sensor was further validated through experimentation with real samples, including watermelon, cantaloupe, cucumber, and zucchini. This study presents the pioneering development of a label-free ICTS sensor, utilizing TCBPE material markers, for rapid and sensitive detection of Ac. This innovative approach offers a robust solution for the swift detecting of Ac in cucurbits and other samples, thereby holding great promise for future applications.
研究领域:
植物病理学
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农业生物技术
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分析化学
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分子生物学
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DOI
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Food Analytical Methods
·
2025
·
IF 3
Li Congwei,Wang Beibei,Kang Kai,Wang Lanyue,Zhao Zhenzhen,Wang Yuping,Ji Xueping
To address ciprofloxacin (CIP) contamination in animal-derived foods, an ultrasensitive electrochemical sensing platform based on a novel ternary heterostructure (Ag-TiO 2 @Zr-TCBPE) was developed through interfacial engineering. The heterointerface was constructed by anchoring a metal–metal oxide electrocatalyst (Ag-TiO 2 ) onto a conductive metal–organic framework (Zr-TCBPE) with a 3D porous hexagonal prismatic structure and high conductivity. This work demonstrates for the first time that the large-scale conjugated architecture of Zr-TCBPE nanotubes enhances CIP enrichment, while the Ag-TiO 2 /Zr-TCBPE heterointerface optimizes electron transport pathways and synergistically promotes CIP oxidation via interfacial electric field-driven effects. Under acidic conditions (pH 5.0), the Ag-TiO 2 @Zr-TCBPE/GCE sensor exhibited optimal performance: a linear range spanning five orders of magnitude (2.5 nmol·L −1 to 150 μmol·L −1 ), a limit of detection (LOD) of 1.6 nmol·L −1 , surpassing most reported CIP sensors. The sensor also showed high anti-interference capability (97.1–107% signal retention), long-term stability (> 96.4% signal retention after 15 days), and satisfactory recovery rates (91.6–103%) in complex matrices (milk, eggs, and river water). This study provides fundamental insights into TCBPE-MOF heterointerface engineering and establishes a robust analytical platform for detecting trace antimicrobials in food and environmental samples.
研究领域:
分析化学
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材料科学
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食品安全
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环境监测
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电化学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Chen Wang,Xing-Jing Zhang,Li−Na Zhao,Ting Zhang,Feng-Ying Bai,Li-Xian Sun,Yong-Heng Xing
The abuse and excessive discharge of organic pollutants such as nitroaromatic compounds (NACs) have become a hot topic of concern for all humanity and society, and the development of fast, effective, and targeted technical means for detecting NACs also faces many challenges. Here, we reported a strontium-based metal–organic framework (MOF) {[Sr2(tcbpe)(H2O)4]}n (Sr-tcbpe), in which tcbpe represents deprotonated 4′,4‴,4″‴,4‴‴-(ethene-1,1,2,2-tetrayl)tetrakis(([1,1′biphenyl]-4-carboxylic acid)). In Sr-tcbpe, Sr–O polyhedron and deprotonated tcbpe4– ligand have a staggered connection to form a self-assembled three-dimensional network structure. In addition, it is found that Sr-tcbpe undergoes no luminescent color change when grinding under solvent protection, while mechanochromic fluorescence behavior is observed when grinding directly, leading to luminescent color changes from cyan to green (Sr-tcbpe-G). Additionally, Sr-tcbpe and Sr-tcbpe-G could selectively detect PNP, DNP, and TNP, and Sr-tcbpe achieves visual fluorescence sensing detection toward TNP at a limit of detection as low as 2.25 μM. Moreover, during the detection process, unexpectedly, TNP exhibits a selective etching effect on Sr-tcbpe, which could drill nano holes with different sizes on the surface area of MOF materials to a certain extent, achieving the conversion of chemical energy to mechanical energy. In addition, the successful preparation of a portable sensor Sr-tcbpe@gypsum block provides a platform for the perfect combination of mechanochromic fluorescence behavior and visualization detection toward TNP. It lays the foundation for the practical application of MOF materials in daily life.
研究领域:
材料科学
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分析化学
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环境科学
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传感器
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DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2026
·
IF 3.9
Chen Wang,Xing-Jing Zhang,Li-Na Zhao,Ting Zhang,Feng-Ying Bai,Yong-Heng Xing
The development of TNP sensors for one-stop real-time detection is crucial for understanding water environments and maintaining human health and safety. However, one of the main obstacles that hindering the development of on-site detection technology in the future is the complexity of the detection environment. Here, in this work, a novel metal-organic framework (MOF) Eu-tcbpe designed with H 4 tcbpe (H 4 tcbpe = 4′,4‴,4‴,4′‴-(ethene-1,1,2,2-tetrayl)tetrakis(([1,1′biphenyl]-4-carboxylic acid))) and Eu 3+ was synthesized. This complex exhibited anti-interference, sensitive, and rapid detection capabilities with the limitation of detection of 0.42 mg/L for TNP. In order to realize real-time portable detection, a hydrogel sensing platform with Eu-tcbpe and methyl red was designed and prepared. This platform retains all the features of Eu-tcbpe . Combining it with smartphone can perform real-time detection towards TNP. It could be found that two independent colorimetric and fluorescent channels on the sensor exhibited specific responses to TNP under sunlight and fluorescence irradiation, respectively. Furthermore, by utilizing the principle of strong electrostatic interaction, the cationic dye safranin T (ST) was encapsulated into Eu-tcbpe with an anionic framework, achieving tunability of the fluorescence color of Eu-tcbpe . This work is expected to provide research ideas for advancing the detection of MOFs in fluorescence sensing and the practical application of MOFs as multi-color luminescent materials.
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ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·Vol. 415
·期 7
·1347-1355
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IF 4.3
Zhang Gan,Zhang Ganggang,Lai Xiaocui,Su Liu,He Weihua,Lai Weihua,Deng Shengliang
Aggregation-induced emission luminogens (AIEgens) are attracting extensive research attention in the biosensor fields. Herein, we report a new polyethyleneimine (PEI)-induced strategy for enhancing luminescence of TCBPE (an AIEgen) to promote its development in biosensor. The copolymer dots (TCBPE-PEI) with high quantum yield (39.7%) and outstanding stability were synthesized via a one-pot method. The fluorescence enhancement mechanism based on the PEI strategy originated from the restriction of intramolecular motions of TCBPEs and the form of donor–acceptor structures to decrease the inherent energy bandgap. Benefiting from chelating property of TCBPE-PEI by Cu 2+ , a fluorescence-quenching sensor for Cu 2+ detection was developed based on the fluorescence quenching of the electron transfer effect. Especially, a good linear range of 10–250 nM with a low limit of detection 1.1 nM was achieved, and it was further applied in samples successfully. The current work provides a novel approach to fabricate AIEgen biosensors and shows great potential in Cu 2+ detection. Graphical Abstract
研究领域:
材料科学
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分析化学
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生物传感器
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纳米技术
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DOI
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高影响因子
Environmental Science-Nano
·
2024
·
IF 5.8
Xinyu Liu,Chenchen Li,Jian Nan,Ruike Liang,Huabin Wang,Rui Xu,Yong Zhang
A combination of metal–organic frameworks (MOFs) with TiO2 can improve the performance of TiO2 in the photocatalytic degradation of organic pollutants. The selection of organic ligands for MOF preparation is a key factor for designing MOF–TiO2 composites with excellent photocatalytic properties. However, the photocatalytic performance of such composites has rarely been examined. In this study, a series of TiO2 nanoflowers (NFs) modified with In-based MOFs were prepared via a hydrothermal method using 1,1,2,2-tetra(4-carboxylbiphenyl)ethylene (TCBPE) as the organic ligand. The fabricated TiO2@In–TCBPE (TIT) heterojunctions exhibited enhanced light absorption, charge separation, and electron transfer characteristics owing to the transport of ·O2− free radicals through the charge transfer pathway between heterostructures. Rhodamine B (RhB) was chosen as a typical representative of organic pollutants under simulated sunlight and is used to evaluate the photocatalytic performance of TIT composites. The optimized TIT composite exhibited a strong photocatalytic effect, and the complete degradation of RhB was achieved in 13 min. In addition, the prepared TIT composite demonstrated excellent photocatalytic activity during the degradation of organic pollutants in different aqueous environments (tap water, lake water, and river water). This study provides a reference for the selection of ligands for the subsequent construction of high-performance MOF photocatalysts.
研究领域:
材料科学
·
环境科学
·
化学
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高影响因子
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