npj Science of Food
·
2025
·Vol. 9
·期 1
·1-9
·
IF 7.8
Ye Xiang,Chen Wen,Yan Fujie,Zheng Xiaodong,Tu Pengcheng
In late-stage type 2 diabetes mellitus (T2DM), impaired islet β cell function leads to absolute insulin deficiency, thereby disrupting blood glucose homeostasis. GLP-1, an incretin hormone, stimulates insulin secretion from islet β cells post-meals. This study investigated the effects of anthocyanin cyanidin-3-O-glucoside (C3G) on GLP-1 secretion using STC-1 (intestinal endocrine L cells) and NIT-1 (islet β cells). In a co-culture system, C3G treatment increased GLP-1 secretion in STC-1 cells, promoting insulin release in NIT-1 cells under high glucose. Mechanistically, C3G activated the PPARβ/δ-β-catenin-TCF-4 pathway in STC-1 cells, enhancing PG precursor transcription and GLP-1 synthesis.Inhibiting PPARβ/δ with GSK0660 blocked this C3G-induced upregulation. Overall, C3G stimulates GLP-1 secretion from intestinal L cells via this pathway, indirectly boosting insulin release from β cells. These findings enhance T2DM mechanism understanding and suggest the potential of C3G in GLP-1-based T2DM therapy.
研究领域:
分子生物学
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代谢疾病研究
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药理学
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PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 9
·1993
·
IF 4.2
Beilei Wang,Dezhi Yang,Xiaohong Zhong,Yuhui Liu,Yong Huang
Abnormal viscosity is closely related to the occurrence of many diseases, such as cancer. Therefore, real-time detection of changes in viscosity in living cells is of great importance. Fluorescent molecular rotors play a critical role in detecting changes in cellular viscosity. Developing red emission viscosity probes with large Stokes shifts and high sensitivity and specificity remains an urgent and important topic. Herein, a novel viscosity-sensitive fluorescent probe (TCF-VIS1) with a large stokes shift and red emission was prepared based on the 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) skeleton. Due to intramolecular rotation, the probe itself does not fluorescence at low viscosity. With the increase in viscosity, the rotation of TCF-VIS1 is limited, and its fluorescence is obviously enhanced. The probe has the advantages of simple preparation, large Stokes shift, good sensitivity and selectivity, and low cytotoxicity, which make it successfully used for viscosity detection in living cells. Moreover, TCF-VIS1 showed its potential for cancer diagnosis at the cell level and in tumor-bearing mice by detecting viscosity. Therefore, the probe is expected to enrich strategies for the detection of viscosity in biological systems and offer a potential tool for cancer diagnosis.
研究领域:
生物化学
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分子生物学
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癌症研究
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荧光光谱学
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生物医学工程
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PubMed
高影响因子
IEEE SENSORS JOURNAL
·
2026
·Vol. 26
·期 9
·13234-13242
·
IF 4.5
Fangzheng Wang,Yunhao Ma,Liyin Hu,Xingjun Yue,Xin Yan
In this work, a high-performance sensor based on a multimode fiber-thin core fiber-multimode fiber (MMF–TCF–MMF) structure is demonstrated. By using a continuous Au film and covalently anchored AgNPs on the TCF surface, the synergistic coupling between exciting surface plasmon resonance (SPR) and localized surface plasmon resonance (LSPR) is realized. Following a systematic optimization guided by COMSOL simulations and experimental validation, the refractive index (RI) sensitivity reached 2525.06 nm/RIU with a figure of merit (FOM) of 9.494 RIU−1, and the temperature sensitivity reached −1.07 nm/°C. The system demonstrated excellent linearity, repeatability, and stability. The proposed sensor effectively merges cost-efficiency with robust precision, showing significant potential for biochemical, environmental, and industrial sensing applications.
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高影响因子
RSC Advances
·
2016
·Vol. 6
·期 60
·55618-55625
·
IF 3.289
Kun Li,Hua Zheng,Hong Zhang,Wen-wen Zhang,Kai Li,Juan Xu
This study made use of a novel totally chlorine-free (TCF) bleaching approach to prepare bleached shellac. Some single factor experiments were employed to investigate the effects of the main factors in the H2O2 bleaching method. A new chlorine-free bleached shellac resin was obtained in the Box–Behnken design responsive surface optimal experimental conditions (pH, 9.0–11.0; 1.33 mL g−1, concentration of 30% hydrogen peroxide; 2.0 h, dropping time of hydrogen peroxide; 7.2 h, bleaching time; 90 °C, temperature). The yield and color index of the bleached shellac obtained under the optimal experimental conditions were 80.84% and 0.5675 respectively. The result of the physicochemical property test, IR spectrum and DSC characterization indicate that the properties of H2O2 bleached shellac are similar to commercially refined shellac resin. Moreover, it is noteworthy that the H2O2 bleached shellac was remarkable in alcohol solubility and thermal lifetime tests. It had a higher acid value and a lower softening point compared with commercially refined shellac resin, which can be applied safely in many fields including food and medical industries without chlorine residues.
研究领域:
材料科学
·
化学工程
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DOI
高影响因子
Surfaces and Interfaces
·
2026
·
IF 6
Yu-long Guo,Peng-Fei Qian,Xin-Yi Luo,Ze-Long Bao,Tong-Yu Li,Qing-Xin Meng,Qinxing Xie,Di Zhang,Hong-Zhang Geng
Currently, silver nanowires (AgNWs) are among the most widely studied materials for flexible transparent electronics. Nevertheless, their practical implementation is hindered by inherent limitations in environmental stability and mechanical robustness. To address these challenges, this study presents a straightforward fabrication approach for a multifunctional flexible transparent conductive film (TCF) that exhibits superior mechanical integrity and long-term stability. Through simple solution processing, multiple functional layers were constructed on a flexible substrate. Among them, AgNWs serve as the conductive layer, SiO 2 NPs as the anti-reflection layer, and AZO as the protective layer. Accordingly, an AgNWs/SiO 2 /AZO TCF (ASZ) was fabricated, exhibiting a sheet resistance of 2.85 Ω/sq, a transmittance of 80.8% at 550 nm, and a figure of merit (FoM) value of 588. This TCF not only combines Joule heating and electromagnetic shielding but can also be used to fabricate OLED devices. A transparent Joule heating device fabricated from this TCF achieves a rapid thermal response, reaching 130 °C at 3 V while maintaining stable operation over extended durations. Furthermore, the film delivers an electromagnetic interference (EMI) shielding effectiveness of 32 dB across the X-band (8.2–12.4 GHz). Critically, owing to the outstanding oxidation resistance and superior surface planarity of the AZO layer, the film’s sheet resistance increases by only 24% after a rigorous 70-day aging test under ambient conditions. When employed as the anode in OLED devices, the TCF enables a maximum luminance of 17,400 cd/m 2 . Collectively, this work establishes a scalable, simple, and highly stable strategy for developing multifunctional transparent electronic devices. Download: Download high-res image (324KB) Download: Download full-size image
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高影响因子
CELL TRANSPLANTATION
·
2021
·
IF 4.064
Xiao-Yu Chen,Sheng-Yong Yang,Xiao-Jie Ruan,Hong-Yue Ding,Ning-Xi Wang,Fang Liu,Jia-Chu Li,Yi Li
Methionine sulfoxide reductase B1 (MsrB1) can catalyze both free and protein-bound R-methionine sulfoxides (R-MetO) to methionine (Met). It has been reported that MsrB1 plays an important role in the development of HCC and human bone osteosarcoma. However, little is known about the functions of MsrB1 in human colorectal cancer (CRC). Herein, we detected MsrB1 expression level in CRC tissue and cell lines, and investigated the effect of MsrB1 knockdown on CRC phenotypes and possible mechanisms involved in. The results showed that MsrB1 was highly expressed in both CRC tissues and cell lines, and that cell proliferation, migration and invasion were significantly inhibited, but apoptosis was increased after MsrB1 knockdown in colorectal cancer HCT116 and RKO cell lines, compared to control siRNA group. In addition, E-cadherin protein level was increased, vimentin and Snail protein were greatly decreased after knockdown of MsrB1 in cells. Furthermore, pGSK-3β (Ser9) and β-catenin protein levels were reduced, the promoter activity of TCF/LEF construction was inhibited after MsrB1 knockdown in cells, suggesting that GSK-3β/β-catenin signaling axis was involved in the tumorigenesis of CRC. In conclusion, the oncogenic role and related mechanisms of MsrB1 in CRC discovered in our work determined the potential role of MsrB1 as a biomarker and may provide a new target for clinical therapy of CRC.
研究领域:
癌症生物学
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细胞生物学
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分子生物学
·
信号转导
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DOI
PubMed
高影响因子
ChemistrySelect
·
2022
·Vol. 7
·期 42
·e202202620
·
IF 2.307
Siyuan Li,Xianrong Yu,Linlin Zeng,Yuhan Xu,Xiaolan Zhao,Wei Tang,Xinrui Duan
Graphical A lysosomal-targeted ratiometric ICT fluorescent probe TCF-LysopH with ideal p K a value (6.12), large Stokes shift (110 nm), and good photostability was developed for real-time tracking lysosomal pH changes during heatstroke. The lysosome is the typical acidic organelle involved in many biological processes, the fluctuation of its pH influences its normal digestive function and even the fate of the cells. To conveniently detect and visualize lysosomal pH changes during heatstroke, we designed and synthesized a lysosomal-targeted ratiometric pH-sensitive fluorescent probe TCF-LysopH. TCF-LysopH showed an ideal p K a value (p K a =6.12) in the lysosomal pH range during heatstroke (5.00–7.00), rapid response for the changes of pH in real-time, reversible pH switching ability, good selectivity, low cytotoxicity, and excellent lysosome-targeting ability. The large Stokes shift of 110 nm allows us to apply the probe for fluorescence imaging in MCF-7 cells. The probe has much better photostability (only less than 20 % decrease in intensity for 15 min irradiation) than LysoTracker Green. The fluorescence intensity became higher with the increase of pH, which is consistent with the tendency of the lysosomal pH change during heatstroke. Importantly, TCF-LysopH was applied to measure the change of lysosomal pH during heatstroke in live cells, the results prove once again that the pH of the lysosome is increased during heatstroke. Thus, TCF-LysopH was successfully developed as a practical and useful tool to provide a better understanding of the complicated mechanisms between lysosome pH change and heatstroke.
研究领域:
生物化学
·
细胞生物学
·
荧光成像
·
分析化学
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DOI
高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 8.2
Zuwei Wang,Huanhuan Guo,Hongbing Wei,Yi Sun,Kai Li,Linfei Yang,Yuanyuan Huang,Wei Yang,Feng Liu,Yueming Li
Modern wireless communication systems put higher requirements on microwave dielectric ceramics in terms of dielectric properties, temperature stability, and low-temperature sintering process. To address the challenges associated with high-frequency signal attenuation, thermal effects, and the high-density integration of multiple-input multiple-output systems, microwave dielectric materials based on the Li2Ti1–x(Fe1/2Nb1/2)xO3 ceramic system were designed and prepared in this study. A monoclinic (cation-ordered)-cubic (cation-disordered) biphasic composite structure was engineered via (Fe1/2Nb1/2)4+ solid solution-induced phase transition, enabling effective tuning toward a near-zero temperature coefficient of resonance frequency (TCF). When x = 0.05, the composite ion synergistic effect significantly suppresses the oxygen vacancy defects and achieves an optimal Q × f value of 65,320 GHz. Furthermore, different proportions of LiF (in the range of 0–8 wt %) were investigated, and the introduction of 4 wt % LiF effectively reduces the sintering temperature of the ceramic to 925 °C, meeting the requirements of the low-temperature cofired ceramic process. The optimized ceramic exhibits excellent dielectric properties (εr = 22.3, Q × f = 70,300 GHz (at 7.32 GHz), TCF = −6.1 ppm/°C) and strong compatibility with silver electrodes. A prototype microstrip patch antenna designed using this ceramic for the BeiDou B1C band (operating at 1.575 GHz) demonstrated high simulated radiation efficiency (96.1%) and gain (4.0 dBi). This study represents significant advancements in TCF precision tuning and low-temperature sintering, offering innovative material solutions for 5G/6G communication devices.
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高影响因子
EXPERIMENTAL CELL RESEARCH
·
2024
·
IF 3.3
Zhen Xu,Yilin Guo,Lu Wang,Jinquan Cui
HECW1 belongs to ubiquitin ligase (E3) HECT family, and is found to be involved in tumorigenesis and tumor progression. However, the function of HECW1 in cervical cancer (CC) remains unknown. Clinical analysis showed that HECW1 is significantly decreased in CC tumor tissues. Ectopic expression of HECW1 suppressed cell growth, promoting cell cycle arrest and apoptosis in CC cells, while downregulation of HECW1 reversed these trends, impeded proliferation and accelerated cell cycle progression of CC cells. Overexpressing of HECW1 reduced mitochondrial membrane potential and the protein expression of voltage-dependent anion channel 1 (VDAC1). In addition, upregulation of HECW1 inhibited nuclear β-catenin accumulation, downregulated β-catenin/TCF/LEF-mediated transcriptional activity and the expression of downstream gene c-Myc, whereas inhibition of HECW1 received opposite results. Further results confirmed HECW1 affects the protein expression of dishevelled-1 (DVL1), a potent activator of Wnt/β‐catenin, and inhibition of HECW1 inhibited the ubiquitination of DVL1, upregulating its expression. Inhibition of DVL1 restrained the promotion effect of HECW1 suppression on cell proliferation. In vivo experiments also verified that HECW1 suppression promoted the tumor formation of CC cells. Summary, we demonstrated that HECW1 inhibits CC cell proliferation and tumor formation by downregulating DVL1 induced Wnt/β‐catenin signaling pathway activation.
研究领域:
肿瘤学
·
分子生物学
·
细胞生物学
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PubMed
高影响因子
DYES AND PIGMENTS
·
2018
·
IF 3.767
Fangyuan Xu,Wei Tang,Saisai Kang,Jinsheng Song,Xinrui Duan
Reactive oxygen species (ROS) are connected to aging and human diseases. The controlled generation of ROS, particularly H 2 O 2 , is necessary to maintain cellular fitness. Fluorescent probe based imaging is the most popular approach for H 2 O 2 detection. The photobleaching of the fluorophores is the significant limitation towards better signal-to-noise ratio and imaging resolution. We design and synthesize a highly sensitive and photo-stable fluorescent probe for detecting H 2 O 2 by using the 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) as an electron acceptor . After reacting with H 2 O 2 , the borate ester group in the probe was transformed into phenol group to form red emitted fluorophore that is highly photo-stable under the irradiation of UV light and excitation laser . A good linear relationship (R 2 = 0.9930) in the range of 1–20 μM of H 2 O 2 was obtained. The limit of detection (3σ/slope) of our probe was 61 nM, which make our probe one of the most sensitive fluorescent probes for H 2 O 2 detection. And the probe doesn't response to common other ROS species. The probe was successfully applied to endogenous intracellular H 2 O 2 imaging in live A549 lung cancer cells. More importantly, the imaging signal just had less than 10% difference in fluorescence intensity after 25 min continuous irradiation of excitation laser. Thus, we believe the proposed TCF-PB is very practical for sensing and imaging of trace amount of H 2 O 2 that endogenously generated in the live cancer cells, which is essential for the study of H 2 O 2 related cell signaling and human diseases.
研究领域:
生物化学
·
细胞生物学
·
癌症研究
·
分析化学
·
成像科学
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高影响因子
危险品化学品经营许可证（带存储）