Journal of Biomaterials and Tissue Engineering
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2023
·Vol. 13
·期 6
·753-764
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IF 0.1
Cheng Xu-Mei,Ma Rui,Zhou Yan-Bing,Li Tao,Lei Sheng-Nan,Zheng Xin,Zhang Hui-Xiao,Liu Mei-Tian,Zhou Jian-Ye,Li Zhi-Qiang
Bone defect repair is a hot topic in tissue engineering, and the development of economical and effective bone scaffold materials to achieve the goal of repairing bone defects is an important task for researchers. In this experiment, fresh bovine cancellous bone was used as a xenogeneic
bone material. After two high-temperature calcinations, β-tricalcium phosphate (β-TCP) material was obtained. An in-situ co-precipitation method was employed to prepare hydroxyapatite+graphene oxide (HA+GO) composite material. Then, the β-TCP material
was immersed in the HA+GO composite material solution to prepare β-TCP+HA+GO composite material. The surface modification of β-TCP material with HA and GO improved the elastic modulus of the raw material. In vitro cell showed that the composite material was non-toxic,
biocompatible, and promoted the proliferation of MC3T3-E1 cells on the β-TCP+HA+GO material. Finally, it was found that β-TCP+HA+GO composite material effectively stimulated the actin and mineralization behavior of MC3T3-E1 cells, and promoted the expression of bone-related
factors such as Alkaline phosphatase (ALP), Collagen I (COLI), Osteocalcin (OCN), Bone Morphogenetic Protein-2 (BMP-2), and Bone Morphogenetic Protein-7 (BMP-7) in MC3T3-E1 cells In conclusion, β-TCP+HA+GO composite material can be applied in the biomedical field as a bone substitute
or repair material.
研究领域:
组织工程学
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生物医学材料
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细胞生物学
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DOI
高影响因子
Biomedical Materials
·
2017
·Vol. 13
·期 1
·015012
·
IF 2.469
Hang Yao,Junpei Kang,Weichang Li,Jian Liu,Renjian Xie,Yingjun Wang,Sa Liu,Dong-An Wang,Li Ren
Cartilage repairing grafts have been widely studied, and osteochondral replacement hydrogels have proven to be an excellent method in research and clinical fields. However, it has been difficult to simultaneously solve three main issues in osteochondral replacement preparation: surface lubrication, overall mechanical support and good simulations of cell regeneration. A novel integrated bilayered hydrogel osteochondral replacement was constructed by blending polyvinyl alcohol (PVA) and β-tricalcium phosphate (β-TCP) in this study. Separated nano-ball milling with ultrasound dispersion prepared β-TCP demonstrated suitable properties of tiny particle size, high purity and ideal distribution, improving the mechanical properties of the novel integrated hydrogel, and providing a cartilage-like lubrication effect and high biocompatibility, including cytocompatibility and osteogenesis. The reinforcement of β-TCP and integrated molding technology enabled the hydrogel to demonstrate excellent component compatibility and good bonding between the two layers, which promoted the strengthening of the compression modulus and tensile modulus up to three times by mechanical testing. The surface lubrication properties of the novel osteochondral hydrogel were similar to the natural cartilage by friction coefficient characterization. The two layers of the novel integrated graft provided a considerable bio-function by co-culturing with chondrocytes and synovium mesenchymal stem cells: chondrocytes promoted adherence achieved by the upper density layer and better osteogenesis performance of the porous lower layer. The design of the bilayered β-TCP/PVA osteochondral hydrogel is promising for use in articular cartilage repair.
研究领域:
生物材料学
·
再生医学
·
组织工程
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DOI
PubMed
高影响因子
ACS Applied Bio Materials
·
2025
·
IF 4.7
Suyun Li,Yanbo Shan,Jingyi Chen,Ruyue Su,Lisheng Zhao,Rujie He,Ying Li
Piezoelectricity is reported to be able to promote bone scaffolds with excellent osteogenic performance. Herein, barium titanate/β-tricalcium phosphate (BTO/β-TCP) piezoelectric composite scaffolds were 3D printed, and their osteogenic performances were investigated in detail. The fabrication of BTO/β-TCP piezoelectric composite scaffolds employed cutting-edge DLP 3D printing technology. The scaffolds, featuring a triply periodic minimal surface (TPMS) design with a porosity of 60%, offered a unique structural framework. A comprehensive assessment of the composition, piezoelectric properties, and mechanical characteristics of the BTO/β-TCP scaffolds was conducted. Notably, an increase in the BTO volume fraction from 50 to 80 vol % within the scaffolds led to a reduction in compressive strength, decreasing from 2.47 to 1.74 MPa. However, this variation was accompanied by a substantial enhancement in the piezoelectric constant d33, soaring from 1.4 pC/N to 21.6 pC/N. Utilizing mouse osteoblasts (MC3T3-E1) in a live/dead cell staining assay, under the influence of external ultrasound, demonstrated the commendable biocompatibility of these piezoelectric composite ceramic bone scaffolds. Furthermore, thorough analyses of alkaline phosphatase (ALP) activity and polymerase chain reaction (PCR) findings provided compelling evidence of the scaffolds’ superior osteogenic properties, underpinning their effectiveness at the cellular protein and gene levels. In conclusion, this study offers a groundbreaking strategy for the employment of BTO/β-TCP piezoelectric composite scaffolds in bone implant applications, harnessing their unique blend of biocompatibility, piezoelectricity, and osteogenic potential.
研究领域:
生物材料学
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再生医学
·
骨科
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF POLYMER ANALYSIS AND CHARACTERIZATION
·
2018
·
IF 1.333
Wei Wu,Jianjun Fang,Wenjuan Liu,Yunhui Zhao,Tianlong Huang,Yanmin Zhao,Xiaofei Li,Jiale Cui,Yun Yang,Zhihua Zhou
Biodegradable polymer/bioceramic composite scaffolds can overcome the limitations of conventional ceramic bone substitutes such as brittleness and difficulty in shaping. A novel degradable scaffold material for bone tissue repairing was developed by combining 1,4-butanediamin-modified poly(lactide-co-glycolide) (BMPLGA) with nano-biaoactive glass and nano-β-tricalcium phosphate (NBAG-β-TCP) . The effects of the inorganic filler content on the morphology, water adsorption, and compressive strength of the nanocomposite scaffolds were investigated. The nanocomposite scaffolds showed an interconnected pore structure with the pore size ranging from 50 to 200 μm. The compressive strength and the water adsorption ability were enhanced with increasing content of the inorganic fillers. The cell proliferation of fibroblast was significantly improved after three and seven days of culture on BMPLGA/NBAG-β-TCP films in comparison with BMPLGA films. The BMPLGA/NBAG-β-TCP composites have a wide application as a potential biomaterial in medical field.
研究领域:
生物医学工程
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材料科学
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组织工程
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产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2016
·
IF 2.758
Huan Zhou,Saisai Hou,Mingjie Zhang,Hong Chai,Yang Liu,Sarit B Bhaduri,Lei Yang,Linhong Deng
Biphasic calcium phosphate (BCP) compositions consisting of β-tricalcium phosphate (β-TCP) and calcium pyrophosphate (CPP) are potential biodegradable ceramics for bone regeneration. The present work demonstrates the formation of such dense ceramics by first preparing the precursors of nano-sized, amorphous , and equiaxed calcium pyrophosphate particles, and then sintering the precursors at 900 °C to transform them into desired BCP. However, if the complex of calcium tripolyphosphate was used, only CPP could be generated. It was also observed that the incorporation of Mg 2+ had several effects on the resulting products including: (1) promoting the generation of meso-porous precipitates; (2) favoring the formation of β-TCP instead of CPP; (3) reducing the grain size and increasing the density of the sintered ceramics, and (4) enhancing the negative electric charge of the BCP surface. Thus, the as-prepared BCP ceramics can serve as potential bone substitute materials in orthopedic applications.
研究领域:
材料科学
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生物医学工程
·
骨科
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
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2023
·Vol. 34
·期 7
·1-13
·
IF 3.7
Feng Jianlang,Liu Junjie,Wang Yingqu,Diao Jingjing,Kuang Yudi,Zhao Naru
The design of hierarchical porous structure in scaffolds is crucial for bone defect regenerative repair. However, bioceramic materials present a challenge in precisely constructing designed micropores owing to the limitation of forming process. To investigate micropore shape influences bone regeneration in bioceramic scaffolds with macropores, hierarchical porous scaffolds with interconnective macropores (~400 μm) and two types of micropores (spherical and fibrous) were prepared using a combination of direct ink writing (DIW) and template sacrifice methods. Compared to the scaffold with spherical micropores, the scaffold with highly interconnected fibrous micropores significantly improved cell adhesion and upregulated osteogenic and angiogenetic-related gene expression in mBMSCs and HUVECs, respectively. Furthermore, in vivo implantation experiments showed that hierarchical scaffolds with fibrous micropores accelerated the bone repair process significantly. This result can be attributed to the high interconnectivity of fibrous micropores, which promotes the transportation of nutrients and waste during bone regeneration. Our work demonstrates that hierarchical porous scaffold design, especially one with a fibrous micropore structure, is a promising strategy for improving the bone regeneration performance of bioceramic scaffolds. Graphical Abstract
研究领域:
生物材料学
·
组织工程
·
骨科医学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Fengxia An,Guangyan Wang,Shuyun Wang,Huan Chen,Jingliang Liu
Perfluorooctanoic acid (PFOA) and 2,4,6-trichlorophenol (2,4,6-TCP) were typical POPs (Persistent Organic Pollutants) in water. In this study, fluorine-doped covalent triazine framework (F-CTF) was synthesized using an ionothermal method, and the adsorption behavior for PFOA and 2,4,6-TCP on F-CTF was investigated using batch experiments and adsorption kinetic tests. Characterization results showed that the large surface area and abundant pore structure of F-CTF provide a large number of adsorption sites for PFOA and 2, 4, 6-TCP. Batch adsorption tests showed that F-CTF adsorbent exhibited high adsorption affinity to PFOA and 2,4,6-TCP, and the adsorption ability could be described well by the Langmuir adsorption model. The maximum adsorption capacity ( q max ) of F-CTF for PFOA and 2,4,6-TCP reached 262.5 mg/g and 709.2 mg/g, respectively. The adsorption process obeyed the pseudo-second-order kinetics. The adsorption mechanism including electrostatic attraction, hydrophobic interaction, and ion-dipole interaction was further confirmed by pH and ionic strength experiments. Additionally, the UV/Na 2 SO 3 /KI system achieved 100 % degradation rate of PFOA and 2, 4, 6-TCP within 40 minutes and the influence factors were further studied. The e aq − was the main active species for the high reduction of PFOA and 2,4,6-TCP in the UV/Na 2 SO 3 /KI system.
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
Journal of Orthopaedic Translation
·
2021
·
IF 5.191
Wen-Xiang Cheng,Yan-Zhi Liu,Xiang-Bo Meng,Zheng-Tan Zheng,Ling-Li Li,Li-Qing Ke,Ling Li,Cui-Shan Huang,Guo-Yuan Zhu,Hu-Dan Pan,Ling Qin,Xin-Luan Wang,Peng Zhang
Objectives Vascularization is an essential step in successful bone tissue engineering. The induction of angiogenesis in bone tissue engineering can be enhanced through the delivery of therapeutic agents that stimulate vessel and bone formation. In this study, we show that cucurbitacin B (CuB), a tetracyclic terpene derived from Cucurbitaceae family plants, facilitates the induction of angiogenesis in vitro. Methods We incorporated CuB into a biodegradable poly (lactide-co-glycolide) (PLGA) and β-tricalcium phosphate (β-TCP) biomaterial scaffold (PT/CuB) Using 3D low-temperature rapid prototyping (LT-RP) technology. A rat skull defect model was used to verify whether the drug-incorporated scaffold has the effects of angiogenesis and osteogenesis in vivo for the regeneration of bone defect. Cytotoxicity assay was performed to determine the safe dose range of the CuB. Tube formation assay and western blot assay were used to analyze the angiogenesis effect of CuB. Results PT/CuB scaffold possessed well-designed bio-mimic structure and improved mechanical properties. CuB was linear release from the composite scaffold without affecting pH value. The results demonstrated that the PT/CuB scaffold significantly enhanced neovascularization and bone regeneration in a rat critical size calvarial defect model compared to the scaffold implants without CuB. Furthermore, CuB stimulated angiogenic signaling via up-regulating VEGFR2 and VEGFR-related signaling pathways. Conclusion CuB can serve as promising candidate compound for promoting neovascularization and osteogenesis, especially in tissue engineering for repair of bone defects. The translational potential of this article This study highlights the potential use of CuB as a therapeutic agent and strongly support its adoption as a component of composite scaffolds for tissue-engineering of bone repair.
研究领域:
生物医学工程
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组织工程
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药理学
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再生医学
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产品关联
DOI
PubMed
高影响因子
International Journal of Bioprinting
·
2023
·
IF 8.4
Changru Zhang,Ya Ren,Weiqing Kong,Yihao Liu,Heyue Li,Han Yang,Bin Cai,Kerong Dai,Chengwei Wang,Liang Tang,Haoyi Niu,Jinwu Wang
Mesoporous bioglass (MBG) with excellent osteointegration, osteoinduction, and biodegradability is a promising material for bone regeneration. However, its clinical application is hindered by complex processing and a lack of personalization, low mechanical strength, and uncontrollable degradation rate. In this study, we developed a double-bond-functionalized photocurable mesoporous bioglass (PMBG) sol that enabled ultrafast photopolymerization within 5 s. By further integrating nanosized tricalcium phosphate (TCP) particles through three-dimensional (3D) printing technology, we fabricated personalized and highly porous PMBG/TCP biphasic scaffolds. The mechanical properties and degradation behavior of the scaffolds were regulated by varying the amount of TCP doping. In vitro and in vivo experiments verified that PMBG/TCP scaffolds slowly released SiO 4 4- and Ca 2+ , forming a vascularized bone regeneration microenvironment within the fully interconnected pore channels of the scaffold. This microenvironment promoted angiogenesis and accelerated bone tissue regeneration. Overall, this work demonstrates the solution to the problem of complex processing and lack of personalization in bioglass scaffolds, and the developed PMBG/TCP biphasic scaffold is an ideal material for bone regeneration applications with broad clinical prospects.
研究领域:
材料科学
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生物医学工程
·
组织工程
·
骨再生
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产品关联
DOI
PubMed
高影响因子
KEY ENGINEERING MATERIALS
·
2018
·
IF 0
Yang Zi Zhao,You Fa Wang
Being one of the three elements of tissue engineering, three-dimensional porous structure scaffold plays an important role in tissue engineering. As it not only prvovide cells for the life, but also serves as a template to guide tissue regeneration and control of organizational structure and other functions. In this study, hyaluronic acid and gelatin are successfully cross-linked by 1-ethyl- (3-dimethylaminopropyl) -carbodiimide hydrochloride (EDC) , and compound β-TCP microspheres to prepare porous hydrogel scaffolds. The microspheres were analyzed by X-ray diffraction (XRD). The scaffolds were characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). At the same time, the compressive strength, swelling ratio, degradation of the scaffold were tested. To assess the in vitro cell compatibility of the scaffolds, mouse L929 fibroblasts were seeded onto scaffolds for cell morphology and cell viability studies. The results showed that the pore size of the porous scaffold can be adjusted by changing the ratio of gelatin to hyaluronic acid (HA), increasing the proportion of hyaluronic acid in a certain range, pore size will be significantly increased. With the increase of the proportion of hyaluronic acid in the scaffold, the swelling ratio and the degradation rate also increased. The compressive strength of the scaffold increased with the increase of the proportion of gelatin. The appropriate ratio of β-TCP can promote cell growth and proliferation.
研究领域:
生物材料
·
组织工程
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
·
2023
·
IF 4.9
Feng Qian,Zongwang Huang,Wenbin Liu,Yanling Liu,Xi He
Segmental bone defects caused by trauma, tumor resection or congenital malformations are often reconstructed with autologous, allogeneic bone grafts or artificial bone materials, of which, about 5% ~ 10% will have delayed healing or even nonunion of fractures. The loss of periosteum and excessive accumulation of ROS in fracture site leads to the aging of osteoblasts and the decline of their proliferation and differentiation, thus affecting the fracture healing process. In this study, we prepared a functional modified artificial periosteum β-TCP/MnO 2 /PCL(β-TMP) by electrospinning with a function of catalyzing decomposition of H 2 O 2 . We examined the surface morphology of β-TMP, the concentration of Ca, P and Mn of β-TMP, as well as the diameter distribution range of nanofibers on β-TMP. Through X-ray diffraction patterns and Fourier transform infrared spectra, β-TMP was characterized and its hydrophilicity was tested. The release of Mn 2+ and Ca 2+ of 0.1 and 0.05% β-TMP in different pH values (7.4 and 5.5) determined by ICP. We also identified that β-TMP could reduce the level of ROS in cells by lowering the level of H 2 O 2 . 0%, 0.05% and 0.1% β-TMP displayed good cell compatibility, cell adhesion and cellular morphology in the condition with or without H 2 O 2 . 0.5% β-TMP showed compromised cell compatibility in normal condition, however, the compromised phenotypes could be partially rescued in the present of H 2 O 2 . Compared with 0%, 0.05% and 0.1% β-TMP displayed higher osteoblastic differentiation with or without H 2 O 2 in BMSCs as well as in MG-63. In sum, β-TMP helped osteogenesis and promoted repair of bone defects.
研究领域:
材料科学
·
再生医学
·
组织工程
·
骨科
✓ 已验证
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2023
·
IF 5.2
Xiang Li,Hanxu Zhang,Hang Zhang
Although β-TCP bioceramics possess favorable biocompatibility and bioactivity, their inherent brittleness restricts further applications. In this study, we aimed to enhance the mechanical performance and biocompatibility of β-TCP by incorporating 15% Akermanite (AK) into the structure. The fabrication process involved the use of digital light processing (DLP) technology to fabricate ceramic green bodies, followed by high-temperature debinding and sintering to obtain the ceramic scaffold. Subsequently, we employed an in-situ growth technique to create micro/nano surface topography on the scaffolds, facilitating synergistic regulation of cell behavior in conjunction with the controlled release of bioactive agents. Scanning electron microscopy (SEM) revealed that the addition of AK enlarged the grain size and reduced the number of micropores in the composite scaffold. Furthermore, after the in-situ growth treatment, a significant increase in micro/nano surface features was observed. Mechanical testing demonstrated that the incorporation of AK effectively improved the mechanical properties of the composite scaffold, resulting in an increase in strength of approximately 20%. Cell experiments indicated that AK supplementation further enhanced the material's cell compatibility, and the presence of micro/nano structures effectively promoted cell adhesion, proliferation, and expression of osteogenic genes. Consequently, we propose a strategy for fabricating porous bioceramics that stimulate bone growth, exhibit high strength, and ameliorate the degradation microenvironment. This approach opens up promising avenues for future applications in bone tissue engineering.
研究领域:
材料科学
·
生物医学工程
·
组织工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）