CHEMISTRY-A EUROPEAN JOURNAL
·
2024
·
IF 3.9
Jialian Ding,Bin Su
Graphical One plus one is greater than two: A remarkable electrochemiluminescence (ECL) enhancement of Ru(bpy) 3 2+ /TPrA is observed by introducing another coreactant, TEOA, with a 10.8-fold increase of ECL intensity and 1.7-fold extension of ECL layer. The enhancement was interpreted by a chemical oxidation route, in which TEOA + ⋅ acts as an enhancer to produce more TPrA + ⋅ and meanwhile allow the remote formation of TPrA + ⋅. This dual-coreactants strategy works not only in solution with freely diffusive Ru(bpy) 3 2+ but also on Ru(bpy) 3 2+ -functionalized microbeads, showing promise in ECL-based bioassay and microscopy. The electrochemiluminescence (ECL) of ruthenium(II) tris(2,2′-bipyridyl) (Ru(bpy) 3 2+ ) with tri- n -propylamine (TPrA) as the good coreactant can be unexpectedly enhanced by a weak coreactant, such as triethanolamine (TEOA). First, the intensity of ECL emitted by Ru(bpy) 3 2+ /TPrA can be remarkably amplified by 10.8-fold after adding some amount of TEOA. Moreover, the ECL layer thickness, measured by self-interference spectroscopy, is also doubled. The enhancement far exceeding the superposition of respective contribution of TPrA and TEOA was elucidated by a “chemical oxidation mechanism”, in which TEOA + ⋅ acts as a chemical enhancer to oxidize TPrA in solution and to accelerate significantly the ECL reaction kinetics. This mechanism was proved by single-photon counting experiment and finite element simulations. In addition, the dual-coreactants strategy works well not only in solution with freely diffusive Ru(bpy) 3 2+ , but also on Ru(bpy) 3 2+ -functionalized microbeads, suggesting that ethanolamines could act as cheap, easily available and low-background enhancers for ECL-based bioanalysis and microscopy.
研究领域:
分析化学
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生物分析
·
显微技术
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产品关联
DOI
PubMed
高影响因子
PHOTOCHEMISTRY AND PHOTOBIOLOGY
·
2021
·Vol. 98
·期 1
·132-140
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IF 3.421
Yuanyuan Peng,Zhipeng Wang,Jhair Peña,Zhiming Guo,Jinfeng Xing
Nanogel is an important kind of biomaterials applied for wound dressings, drug delivery, medical diagnostics and biosensors. The properties of nanogels closely depend on the density of the crosslinking network. In this study, the role of triethanolamine (TEOA) in the effect on the crosslinking degree of nanogels based on poly(ethylene glycol) diacrylate (PEGDA) was investigated and illustrated. The effect of TEOA on the process of photopolymerization at 532 nm and properties of the nanogels was systematically investigated by using UV-vis spectroscopy, FT-IR spectroscopy, 1 H NMR, DLS, SEM, AFM and DSC. In brief, the double-bond conversion of photopolymerization and the crosslinking degree of nanogels can be effectively regulated by TEOA.
研究领域:
材料科学
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高分子化学
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生物材料
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纳米技术
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 8
·1-9
·
IF 5.7
Hao Xuanxuan,Liu Zhimin,Fan Yunfeng,Wang Jie,Cui Chen,Hu Leqian
A novel electrochemiluminescence (ECL) aptasensor, using graphite carbonitride (g-C 3 N 4 ) capped CdS quantum dots (CdS QDs@g-C 3 N 4 ) and Au nanoparticles decorated triethanolamine (AuNPs@TEOA) as dual coreactants, was proposed for the determination of mucin 1 (MUC1). Higher ECL efficiency was acquired due to the double enhancement contribution of CdS QDs and TEOA to Ru (bpy) 3 2+ ECL. Additionally, AuNPs@TEOA also acted as nanocarrier for MUC1 aptamer immobilization. After the aptasensor was incubated in target MUC1, the decreased ECL emission was obtained because of the poor conductivity of MUC1. The ECL aptasensor displayed a good linear correlation for MUC1 in the range 0.1 pg mL −1 -1000 ng mL −1 , and the detection limit was 33 fg mL −1 . MUC1 spiked into human serum samples was quantified to assess the practicability of the ECL aptasensor. Graphical abstract
研究领域:
分析化学
·
生物传感器
·
癌症诊断
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Xiu-hua Wei,Xing Qiao,Jie Fan,Yuan-qiang Hao,Yin-tang Zhang,Yan-li Zhou,Mao-tian Xu
Herein, a label-free electrochemiluminescence (ECL) biosensor for sensitive determination of carcinoembryonic antigen (CEA) was fabricated based on CdS quantum dots loaded onto metal organic framework (CdS QDs@MOF) and triethanolamine modified on gold nanoparticles (TEOA@AuNPs) as bi-coreactants of Ru(bpy) 3 2+ ECL system. The TEOA@AuNPs served as not only coreactant of Ru(bpy) 3 2+ but also nanocarrier for CEA aptamer which was bonded to the TEOA@AuNPs through Au–S bond. After incubated in CEA solution, the ECL signals decreased due to the poor conductivity of CEA and there was a linear correlation between the ECL intensity and log(C CEA ). Based on this result, a label-free ECL biosensor has been developed successfully and used to detect CEA with a detection limit of 8.5 × 10 −5 ng/mL. The proposed ECL aptasensor had good analytical performance in the detection of CEA in human serum samples.
研究领域:
生物传感
·
医学检测
·
纳米技术
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产品关联
DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2021
·
IF 4.291
Junjie Li,Yuanyuan Peng,Jhair Peña,Jinfeng Xing
Nanogels based Polyethylene glycol diacrylate (PEGDA) attracted much attention due to their promising application in drug delivery and tissue engineering. Here, PEGDA photopolymerization at 532 nm with different initiating systems was investigated. Nanogels can be obtained using single 2′,4′,5′,7′-tetrabromofluorescein disodium salt (EY) as the photoinitiator. To increase the polymerization rate, EY, triethanolamine (TEOA), and diphenyl iodonium hexafluorophosphate (DPI) were investigated as a three-component initiating system. With that initiating system, the conversion of PEGDA double bond was higher than 90 % in 1 min at extremely low irradiation energy density (P= 0.95 mW/cm 2 ). However, the conversion of PEGDA double bond was only 51.5 % in 1 min at the same irradiation energy density when using EY and TEOA as two-component initiating system. Subsequently, the corresponding mechanism of the three-component (EY-TEOA-DPI) initiating system was illustrated. DPI might participate in two ways of photooxidation and reduction, which dramatically improved the polymerization rate. The EY-TEOA-DPI initiating system is promising to be applied in the preparation of multifunctional hydrogels.
研究领域:
材料科学
·
高分子化学
·
生物医学工程
·
药学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）