SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Qinghua Hu,Tao Gong,Yu Mao,Qiang Yin,Yuyuan Wang,Hongqing Wang
In this paper, we specifically designed and synthesized an excellent colorimetric and ratiometric fluorescent sensor DPA-CI for rapid and convenient detection of the highly toxic phosgene. DPA-CI was developed by incorporated a diphenylamine (DPA) and a 2-imine-3-benzo[d]imidazole as the enhanced push–pull electronic structure into the coumarin fluorophore matrix. The sensor DPA-CI towards phosgene sensing exhibited both visible colorimetric and ratiometric fluorescent color change in solution and in gaseous conditions with TICT and AIE mechanism respectively, which can be easily distinguished by using the naked eye. Also, the sensor DPA-CI showed splendid sensing performance such as excellent selectivity, rapid response (less than 8 s in THF and 2 min in gaseous condition), and fair sensitivity (limit of detection less than 0.11 ppm in gaseous condition and 0.27 μM in solution). The design strategy based on enhanced push–pull electronic structure with AIE and TICT properties will be helpful to construct a solid optical sensor with excellent potential application prospects for portable and visual sensing of gaseous phosgene through distinct color and ratiometric fluorescence change by the naked eyes.
研究领域:
化学
·
材料科学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Taihe Han,Shuai Mu,Qin Huang,Jiaxing Sun,Xin Lv,Yanxia Zhang,Xiaoyan Liu,Wei Guo,Haixia Zhang
The development of high fluorescence quantum yield molecular fluorophores for second-order near-infrared (NIR-II) imaging is highly desirable but remains challenging due to the lack of reliable design guidelines. We report a method to construct “J-aggregates” by combining the inhibition of twisted intramolecular charge transfer (TICT) and electron-withdrawing strategies. Introducing electron-withdrawing substituents at the middle position of the classic boron dipyrromethene (BODIPY) backbone promotes J-type slip stacking, enhancing NIR-II fluorescence through increased electrostatic repulsion and intermolecular hydrogen bonding. The 3,5-aryl position is modified with sulfone and quaternary piperazine groups. The negative inductive effect (–I effect) from strong electron-withdrawing action increases the ionization potential (IP) of the amino group, inhibiting the TICT process and improving the quantum yield to 9.03 % and 16.9 %, respectively. Notably, the fluorescence quantum yield of NBDP-SO 2 increased to 18.2 % after self-assembly with F127, successfully applied in bioimaging. This study provides a new method for constructing high fluorescence quantum yield NIR-II emission by manipulating molecular stacking and establishing the relationship between molecular structure, intermolecular interactions, and fluorescence properties.
研究领域:
生物医学工程
·
分子影像学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Jian-Bo Chen,Baosheng Li,Ying Xiong,Jie Sun
Two new fluorescent probes based on a coumarin core have been developed for the detection of nitramines (RDX and HMX) via responding to their photo-degradated nitrogen dioxide (NO 2 ). Although both probes contain the same fluorophore and recognition site, they display different fluorescent responses to NO 2 and nitramines. 8- O xime-7-dimethylamino-coumarin (8-ODC) emits traditional turn-on fluorescence toward NO 2 , which is based on the C N isomerization. In contrast, 3- O xime-7-dimethylamino-coumarin (3-ODC) exhibits a unique turn-off response with strong fluorescence quenching (about 98%) as a result of a twisted intramolecular charge transfer mechanism (TICT). In addition, 3-ODC offers a highly selective and sensitive determination of nitramines via detecting NO 2 in solution. The limit of detection of 3-ODC for NO 2 , HMX and RDX was 90 ppb, 60 nM and 90 nM, respectively. Notably, main interferents such as TNT, PETN, NO, NO 2 + and H 2 O 2 showed no obvious interference to the detection of NO 2 and nitramines. Therefore, a novel fluorescent probe (3-ODC) based on the TICT mechanism was firstly developed to detect NO 2 and nitramines by fluorescence quenching with high selectivity and sensitivity.
研究领域:
分析化学
·
环境科学
·
爆炸物检测
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF LUMINESCENCE
·
2021
·
IF 3.599
Jianai Chen,Sijie Tan,Yue Yu,Shitong Zhang,Weijun Li,Qingbao Song,Yujie Dong,Cheng Zhang,Wai-Yeung Wong
A coplanar donor-acceptor (D-A) structure in single crystal is directly observed for TICT-Type DPA-PYZ, which corresponds to a coplanar localized excited (LE) state. The conversion between coplanar LE and twisted intramolecular charge transfer (TICT) excited state in the solid state can be achieved through the change of the D-A twist angle between ~0° and ~30° during grinding, heating and solvent fumigation processes. This twist angle change between donor and acceptor was proved by the Raman, FT-IR spectroscopy and X-ray crystallographic analysis as well as theoretical calculation. This research provides the clear evidence for the conversion between coplanar LE and TICT excited states of D-A molecules in solid state and contributes much to the deep understanding of stimulus-response behavior of fluorescent D-A molecular solids from the viewpoint of excited state control.
研究领域:
化学
·
材料科学
·
光物理学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2023
·
IF 10
Liang Zhao,Han Zhu,You-Yang Duo,Dai-Wen Pang,Zhi-Gang Wang,Shu-Lin Liu
The serious threat that cancer poses to human health highlights the significance of early detection and effective treatment. The integration of fluorescence diagnosis and photothermal therapy in NIR-II has gained attention due to its high sensitivity, fast response, and non-invasiveness. Fluorescence, produced by the radiative relaxation process of electrons in a molecule, and photothermal, generated by the non-radiative relaxation process of electrons in a molecule, are competing photophysical processes. Hence, it is a challenge for the molecule to balance between the properties of fluorescence and photothermal. In this study, we designed a NIR-II hemicyanine with TICT character to obtain molecules with both better fluorescence and photothermal properties, utilizing positively charged pyridine salt and triphenylamine as electron acceptor and donor, respectively, and oxole as the conjugated π-bridge. HCY-995 , one of the synthesized compounds, had a quantum yield of 0.09%, photothermal conversion efficiency of 54.90%, and a significant Stoke shift of 232 nm, which made it appropriate for the integration of photothermal therapy and high-resolution imaging. This study provides new insights into the development of NIR-II molecules with fluorescent and photothermal integrated properties. This article is protected by copyright. All rights reserved
研究领域:
生物医学工程
·
分子影像
·
癌症治疗
✓ 已验证
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Peng Zhang,Xinjie Guo,Yuzhe Xiao,Qian Zhang,Caifeng Ding
Accurate detection of human serum albumin (HSA) in biological samples is quite meaningful for early disease diagnosis and treatment. Herein, a novel fluorescent probe 1-ethyl-4-[2-[4-(diethylamino)-2-hydroxyphenyl]ethenyl]]-pyridinium salt (DEHP) was developed for HSA determination. The inherent fluorescence of DEHP is essentially negligible at physiological conditions assigned to the well-developed twisted intramolecular charge transfer (TICT) protocol. An intriguing fluorescence light up is triggered as the addition of HSA, on account of the inhibited TICT procedure when DEHP enters the hydrophobic cavity of protein HSA. This combination leads to a turn on fluorescent response for HSA with a detection limit of 4.8 nM. After an overall investigation, it has been proved that the strong binding between DEHP and HSA is specific-site-related. In additional, the probe implies a great potential to assist clinical diagnosis due to the usage in actual serum detection. Cell imaging also shows that the probe is expected to monitor HSA production process at cell level.
研究领域:
分析化学
·
生物化学
·
分子生物学
·
临床诊断
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Shangke Liu,Ziming Yu,Liping Su,Panyi Hu,Youxiang Ren,Shiyu Liu,Haitao Ding,Shiqian Qi,Mengchang Yang,Min Zhang,Xiaohe Tian
Mitochondrial structural remodeling under oxidative stress is central to the pathogenesis of skin photoaging. Here, we report Mito-Py, a robust mitochondria-targeted probe with a special functional donor-π-acceptor (D-π-A) configuration. Featuring a large Stokes shift (∼130 nm) for minimal self-absorption and viscosity-sensitive emission via a twisted intramolecular charge transfer (TICT) mechanism, Mito-Py enables real-time mapping of mitochondrial viscosity fluctuations during UV-induced oxidative stress in HaCaT keratinocytes. Unlike membrane-potential-dependent dyes, Mito-Py achieves membrane-potential-independent mitochondrial retention, ensuring stable suborganellar localization even during depolarization events. Its exceptional resistance to photobleaching permits super-resolution structured illumination microscopy (SIM) to resolve UV-induced cristae fragmentation with ultrahigh spatial precision (sub-100 nm). Beyond cellular models, Mito-Py was applied to paraffin-embedded skin sections from a UV-induced murine photoaging model, where staining revealed markedly stronger fluorescence in photoaged skin compared to normal skin. These results underscore Mito-Py’s capability to detect mitochondrial alterations in complex tissue environments, providing a powerful, photostable tool for dissecting mitochondrial stress responses and exploring organelle crosstalk in photoaging and related dermatological disorders.
研究领域:
生物化学
·
细胞生物学
·
生物物理学
·
荧光成像
·
皮肤科
·
光生物学
✓ 已验证
DOI
高影响因子
ChemistrySelect
·
2024
·Vol. 9
·期 9
·e202303466
·
IF 1.9
Xiaochen Gao,Ke Li,Yu Xiao,Jianan Dai,Zheyu Shen,Guangping Lv,Yaqiong Yang
Graphical In this research, a new TICT probe (IDL-FP) was designed for sensitive detection of protamine. The introduction of indole-3-carboxaldehyde and 4-dimethylaminobenzaldehyde gave IDL-FP both an electron-donor and electron-acceptor, which urged the complete intramolecular charge transfer and a large stokes shift, allowing IDL-FP to process minimal background noise and excellent biocompatibility. Additionally, protamine can directly induce the aggregation of IDL-FP caused by electrostatic attraction. In this way, the turn-on detection of protamine was achieved and the detection limit was as low as 8.87 ng mL −1 , which showed a better sensitivity and higher stability. As IDL-FP exhibited good stability in physiological pH environment, we successfully applied IDL-FP to detect spiked-in protamine in human serum. In summary, probe IDL-FP is a promising tool for the quantitative determination of protamine for potential clinical usage. As effective methods to accurately and rapidly detect protamine remains a major challenge, here we present a fluorescent protein chromophore-based probe (IDL-FP) utilizing twisted-intramolecular charge transfer (TICT) to detect protamine. The introduction of an electron-donor group 4-dimethylaminobenzaldehyde extends the emission wavelength of IDL-FP to the red region. The deprotection of t-butyloxy carbonyl groups can then generate an electronegative carboxyl group, which promotes the binding to protamine via electrostatic attraction. Thus, IDL-FP is sensitive and specific towards protamine and can serve as a highly selective detector for protamine with a detection limit as low as 8.87 ng/mL. As IDL-FP exhibited good stability in physiological pH environment, we successfully apply IDL-FP to detect spiked-in protamine in human serum. In summary, probe IDL-FP is a promising tool for the quantitative determination of protamine.
研究领域:
生物化学
·
医学检测
·
分析化学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Lingchen Meng,Shan Jiang,Meiyu Song,Fei Yan,Wei Zhang,Bin Xu,Wenjing Tian
Fluorescent thermometers with near-infrared (NIR) emission play an important role in visualizing the intracellular temperature with high resolution and investigating the cellular functions and biochemical activities. Herein, we designed and synthesized a donor−Π–acceptor luminogen, 2-([1,1′-biphenyl]-4-yl)-3-(4-((E)-4-(diphenylamino)styryl) phenyl) fumaronitrile (TBB) by Suzuki coupling reaction. TBB exhibited twisted intramolecular charge transfer-based NIR emission, aggregation-induced emission, and temperature-sensitive emission features. A ratiometric fluorescent thermometer was constructed by encapsulating thermosensitive NIR fluorophore TBB and Rhodamine 110 dye into an amphiphilic polymer matrix F127 to form TBB&R110@F127 nanoparticles (TRF NPs). TRF NPs showed a good temperature sensitivity of 2.37%·°C–1, wide temperature response ranges from 25 to 65 °C, and excellent temperature-sensitive emission reversibility. Intracellular thermometry experiments indicated that TRF NPs could monitor the cellular temperature change from 25 to 53 °C for Hep-G2 cells under the photothermal therapy agent heating process, indicating the considerable potential applications of TRF NPs in the biological thermometry field.
研究领域:
生物医学工程
·
材料科学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Zhongyong Xu,Xiongfei Zhao,Mei Zhou,Zhijun Zhang,Tianyi Qin,Dong Wang,Lei Wang,Xiaojun Peng,Bin Liu
Increasing demand for detecting environmental and biological analytes in aqueous solutions based on fluorescent probes has raised the bar for the fluorophores used. Especially, the twisted intramolecular charge transfer (TICT) state of fluorophore can trigger the formation of a charge-separated non-emissive specie, leading to a low fluorescence brightness. In this work, we performed a simple donor engineering on the flavonoid-based fluorophores to regulate the TICT formation. A systematic study on relationship between donor structure and photophysical properties has been carried out. Among a series of flavonoids, the methylpiperazine-substituted one exhibited the strongest fluorescence brightness in water since the methylpiperazine could rigidify the rotation of donor moiety to suppress TICT state. Moreover, a novel fluorescent probe based on this flavonoid successfully realized the cysteine detection both in water and in living cells. This work confirmed the validity of design strategy by inhibiting TICT to enhance the fluorescence brightness, and also provided a practical flavonoid-based fluorophore for further bioimaging applications.
研究领域:
化学
·
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Guangfa Wang,Zhixin Wan,Zhenzhen Cai,Jiguang Li,Yushu Li,Xiaoyun Hu,Da Lei,Xincun Dou
Inhibition of twisting intramolecular charge transfer (TICT) is one of the most attractive methods for fluorescence-on analysis, whereas it remains enigmatic whether the fluorescence in a TICT-based probe could be thoroughly lightened. Here, for maximizing the fluorescence-on signal of the TICT-based probe, we develop a model by employing chemical reaction to directly cleave the linkage between the rotational electron donor and acceptor with a predisposed fluorescent signal close to zero. To validate this assumption, a nonfluorescent probe with barrierless rotation is successfully achieved by grafting acryloyl with −C═C– recognition sites onto coumarin, and 7-hydroxycoumarin with bright blue fluorescence could be released within 3 s upon probing KMnO4 with an amount as low as 0.95 nM and 6.6 pg. We believe that the present strategy could not only deepen the insights of photochemistry but also facilitate the development of a theranostic drug delivery system, energy conversion, pollution control, and health risk reduction.
研究领域:
光化学
·
分析化学
·
药物递送系统
·
环境科学
·
健康科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2022
·
IF 5.122
Meijuan Zhang,Jian Cao,Cuiping Huang,Mingming Liu,Yanan Li,Chuanxiao Wang,Yajing Tu
Human serum albumin (HSA) accounts for 60% of total plasma protein and is closely related to life-threatening diseases. In this strategy, three fluorescent sensors of Nap-OH, Nap-C and Nap-NO 2 based on twisted intramolecular charge transfer (TICT) are designed and synthesized for detecting of HSA, and the effect of electron donating-withdrawing groups of sensors on the detection of HSA is studied. After added HSA, the fluorescent emission of Nap-NO 2 is turned on at 630 nm, and the color changed from colorless to pink. Nap-NO 2 is characterized by a 14.06-fold increase in fluorescence emission and has high sensitivity, good selectivity, fast response, and good stability. Nap-NO 2 could effectively distinguish HSA from other proteins, including bovine serum albumin (BSA). Drug competition experiments are performed that Nap-NO 2 can bind to the IB domain of HSA, avoiding the interference of commonly used drugs. Nap-OH, Nap-C and Nap-NO 2 can also be used for viscosity detection.
研究领域:
生物化学
·
医学检测
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）