In this study, lipase from Thermomyces lanuginosus (TLL) was immobilized onto the parent and organic groups modified SBA-15, and the enzymatic properties of the obtained immobilized TLL samples were investigated. 1) Activity of SBA-15-TLL at 2862.78 ± 293.24 U/g was obtained. 2) Most of the organic groups modification favored a great improvement in activity, and higher activity over 12000 U/g was observed for N-phenylaminomethyl and phenyl group modification. 3) Most of the supported TLL showed better thermostability in air while poor in phosphate buffer, with over 80% vers less than 20% of their initial activity retained after 4 h incubation at 70℃. 4) The n-dodecyl, phenyl and N-phenylaminomethyl group functionalization decreased the sensitivity of immobilized TLL in extreme pH values. 5) The n-octyl and 2-(propoxymethyl)oxirane group modification confered the supported TLL good reusability, and over 60% of their initial activity was retained after five successive cycles of reuse.
The present study aims to fabricate efficient immobilized lipases for medium and long-chain triacylglycerols preparation through acidolysis. Eighteen novel materials, including five mesoporous materials, four organic groups modified SBA-15 samples (R-SBA-15) and nine macroporous resins, were employed as matrices for lipases immobilization. Lipases of TLL, AOL, RML and CALB were evaluated. Results indicated that TLL exhibited better acidolysis performance and decanoic acid content from 30% to 45% could be obtained; while other lipases (CALB, RML, and AOL) showed poor performance. In addition, macroporous resins of DA-201, ADS-17, D3520, and mesoporous silicas of SBA-15, Al-MCM-41, HMS, were suitable for TLL immobilization. TLL@SBA-15 was selected for acidolysis and the reaction conditions were optimized through single factors study and orthogonal design. The optimum conditions for the acidolysis were: lipase amount 6 wt%, decanoic acid: camellia oil molar ratio 14:1, reaction time 12 h and temperature 50°C. Under these conditions, decanoic acid content at 59.87%, oleic acid content 33.99% and sn -2 decanoic acid content 7.41% was obtained (the mentioned fatty acids here were the fatty acids combined to oil molecules, not free fatty acids). Graphical Eighteen novel materials, including five mesoporous materials, four organic groups modified SBA-15 samples (R-SBA-15) and nine macroporous resins, were employed as matrices to support TLL, AOL, RML and CALB for MLM oil preparation through acidolysis. Good performance was observed from TLL@SBA-15, and decanoic acid content at 59.87%, oleic acid content 33.99% and sn-2 decanoic acid content 7.41% could be obtained under optimized conditions.
This study aims to fabricate immobilized lipases for efficient preparation of 1,3-dioleoyl-2-palmitoyl-glycerol (OPO) through acidolysis of glycerol tripalmitate (PPP). Twelve (three types) supports and five lipases were studied carefully. Among them, the immobilized Thermomyces lanuginosa lipase (TLL) samples exhibited overall better performance than that of other immobilized lipases. Particularly, organic groups functionalized SBA-15 (R-SBA-15) supported TLL (TLL@R-SBA-15) samples gave PPP conversion from 97.70 to 99.00 % and OPO content from 59.52 to 64.73 %. After optimization, PPP conversion up to 99.07 %, OPO content 73.15 % and sn -2 palmitic acid content 90.09 % were obtained with TLL@C 18 H 37 -SBA-15 as catalyst. Moreover, TLL@C 18 H 37 -SBA-15 exhibited better acidolysis performance from 50 °C than that from 60 to 80 °C, which helped inhibit acyl migration. In addition, after 5 cycles of reuse, TLL@C 18 H 37 -SBA-15 retained 81.04 % (based on OPO content) and 98.88 % (based on sn- 2 palmitic acid content) of its initial activity, indicating it had an attractive prospect in future applications.
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