Blood uric acid (UA) levels are closely associated with various diseases. This study presents a novel UA photothermal detection system. UA reacts with uricase to generate hydrogen peroxide, which then oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) into oxidized TMB (oxTMB) in the presence of copper ions as the catalyst. Crucially, instead of expensive laser, Light Emitting Diode (LED) was applied to offer the irradiation on oxTMB, inducing a temperature increase. The increment of temperature measured by a handheld household thermometer exhibits a linear correlation with the concentration of UA in the samples. This method demonstrates enhanced detection sensitivity and excellent anti-interference capabilities. It has been successfully applied to precisely determine UA levels in volunteer blood samples. The sensing platform achieves portable UA detection in human blood within 3 min, featuring a linear response range of 50–400 μM, a detection limit (LOD) of 14.4 μM. This work establishes a novel miniaturized LED-based photothermal (LED-PT) uric acid detection device, holding significant potential for rapid, portable UA monitoring in healthcare and clinical diagnostics.
It was found that Hg 2+ can inhibit the peroxidase mimetic activity of bovine serum albumin (BSA) protected Au clusters (BSA-Au) due to the specific interaction between Hg 2+ and Au + existed onto the surface of BSA-Au clusters. By coupling with 3, 3′, 5, 5′-tetramethylbenzidine (TMB)–H 2 O 2 chromogenic reaction, a novel method for Hg 2+ detection was developed based on the inhibiting effect of Hg 2+ on BSA-Au clusters peroxidase-like activity. This method exhibited high selectivity and sensitivity. As low as 3 nM (0.6 ppb, 3 σ ) Hg 2+ could be detected with a linear range from 10 nM (2 ppb) to 10 µM (2 ppm) and this method was successfully applied for the determination of total mercury content in skin lightening products.
危险品化学品经营许可证（带存储）