MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Yu Han,Ting Zhang,Lin Zhu,Jiawei Liu,Jianjun Liao,Long Wu
Artificial antioxidants (AOs), including BHT, BHA, PG, and TBHQ, are extensively utilized in food, pharmaceutical, and other industries to prevent oxidation and prolong the shelf life of products. However, excessive intake of these AOs may bring about potential health risks, such as allergic reactions or interference with normal physiological functions. As a result, there is a pressing need for sensitive, simple, and simultaneous detection methods for AOs. Electrochemical sensors, which usually involves modification of electrodes with complex procedures and delicate operations, has its practicality in real-world applications somewhat restricted. Herein, we developed a versatile detection system based on the catalytic activity of Pt Au nanoflowers (Pt Au NF) in the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). Specifically, the Pt Au NF/TMB reaction system produces a distinct blue color, which progressively fades with AOs. This colorimetric response offers a straightforward, naked-eye readout for AOs detection. Moreover, the Pt Au NF/TMB system can also be employed for localized electrochemical detection using the redox activity of TMB. By simply mixing Pt Au NF and TMB in a one-step procedure, we established two complementary detection modes: naked-eye readout and localized electrochemical detection. The differential pulse voltammetry (DPV) mode displayed satisfactory analytical performance towards BHT, BHA, PG and TBHQ, with linear ranges of 0.1 to 200 mg/L and detection limits of 0.063, 0.048, 0.025 and 0.016 mg/L (3σ/slope), respectively. The integration of “one-step mixing” and “one-drop detection”, therefore, greatly reduces operation complexity, providing an accurate and efficient platform for AOs detection, as well as a valuable tactic for combined detection in food samples.
研究领域:
分析化学
·
纳米技术
·
食品安全
·
生物传感
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2021
·Vol. 45
·期 38
·18048-18053
·
IF 3.591
Rui Shi,Qiaoling He,Shiqi Cheng,Bolin Chen,Yilin Wang
MoS2 nanosheets (MoS2 NSs), which exhibited peroxidase-like activity, were prepared using the hydrothermal method. The microstructure and element speciation of the MoS2 NSs were characterized using transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS), respectively. The obtained MoS2 NSs could catalyze the H2O2-mediated oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to form blue oxidized TMB (ox TMB) with an absorption peak at 652 nm. The effects of MoS2 NS concentration, incubation time, temperature, and acidity on the color development of TMB were investigated. Under the optimal conditions (1.0 μg mL−1, 40 min, 40 °C, pH 4.0), the increase in absorbance, which was induced by MoS2 NS-catalyzed TMB oxidation, enabled the detection of H2O2 ranging from 2.5 μmol L−1 to 300 μmol L−1. As H2O2 is one of the products of glucose oxidase (Gox)-catalyzed glucose oxidation, a colorimetric method was further established for measuring the concentration of glucose. The absorbance response was linearly dependent on glucose concentration of 2.5–200 μmol L−1, with a detection limit of 2.1 μmol L−1. The proposed method was applied to the assay of glucose in urine samples with satisfactory results.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 29
·13952-13962
·
IF 3.3
Dehong Bai,Ziyu Xue,Shaohui Li,Ran Meng,Dongxia Zhang,Xibin Zhou
Chromate (Cr(VI)) is a highly toxic heavy metal ion that not only endangers human health but also causes environmental pollution. To control and remove Cr(VI) from water more effectively, we report a simple, novel, and rapid detection technique for Cr(VI) in water based on an iron-manganese bimetal-metal organic framework (FexMny-MOF). Firstly, the synthesized FexMny-MOF has a large specific surface area and porosity; for the first time, an MOF with dual mimic enzyme properties is reported, which can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to the blue product in the presence or absence of H2O2. Interestingly, the mimic enzyme properties of FexMny-MOF are enhanced than those of Fe-MOF and Mn-MOF. The free energies of elementary reactions and adsorption energies (Eads) of H2O2 and O2 adsorbed on different materials were calculated by density functional theory (DFT) to verify the mechanism of action of FexMny-MOF. The Fe1Mn1-MOF mimics oxidase-like oxidation of TMB; 8-hydroxyquinoline (8-HQ) is used as the inhibitor of TMB oxidation, and the specific interaction between 8-HQ and Cr(VI) weakens the inhibition of 8-HQ on TMB oxidation to achieve the detection of Cr(VI). The established method has high sensitivity and selectivity with a detection limit of 51 nM and a linear range of 80–3500 nM. This method has been successfully applied to the determination of Cr(VI) in tap water and the Yellow River, showing its great potential in environmental monitoring.
研究领域:
环境科学
·
材料科学
·
生物化学
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产品关联
DOI
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2016
·Vol. 18
·期 3
·1-10
·
IF 2.101
Guo Leilei,Huang Kaixun,Liu Hongmei
Selenium nanoparticles (SeNPs) are considered to be the new selenium supplement forms with high biological activity and low toxicity; however, the molecular mechanism by which SeNPs exert the biological function is unclear. Here, we reported that biocompatibility SeNPs possessed intrinsic oxidase-like activity. Using Na 2 SeO 3 as a precursor and glutathione as a reductant, biocompatibility SeNPs were synthesized by the wet chemical reduction method in the presence of bovine serum albumin (BSA). The results of structure characterization revealed that synthesized SeNPs were amorphous red elementary selenium with spherical morphology, and ranged in size from 25 to 70 nm size with a narrow distribution (41.4 ± 6.7 nm). The oxidase-like activity of the as-synthesized SeNPs was tested with 3,3′,5,5′-tetramethylbenzidine (TMB) as a substrate. The results indicated that SeNPs could catalyze the oxidization of TMB by dissolved oxygen. These SeNPs showed an optimum catalytic activity at pH 4 and 30 °C, and the oxidase-like activity was higher as the concentration of SeNPs increased and the size of SeNPs decreased. The Michaelis constant ( K
m ) values and maximal reaction velocity ( V
max ) of the SeNPs for TMB oxidation were 0.0083 mol/L and 3.042 μmol/L min, respectively.
研究领域:
纳米生物学
·
生物化学
·
医学
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DOI
高影响因子
ACS Applied Nano Materials
·
2020
·
IF 0
Chun-Xia Zhang,Yan-Cai Gao,Hong-Wei Li,Yuqing Wu
Guanosine monophosphate (GMP)-protected bimetallic nanoclusters of gold and platinum, Au–PtNCs-GMP, were prepared through an improved hydrothermal method under basic conditions, which show high catalytic activity for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) under ambient conditions. Further systematic optimization of synthesis provides the best catalyst by feeding the materials 0.8 mM HAuCl4, 0.2 mM H2PtCl6, 4.0 mM NaOH, and 3.0 mM GMP, which reacted at 120 °C for 30 min, which show an initial maximum velocity (Vmax) of 253.8 × 10–8 M s–1 for TMB as the substrate. Such a value is at the top among the oxidase mimetics reported, which is essentially attributed to the synergistic effect between two metals in the small nanocluster. Moreover, the achieved Au–PtNCs-GMP show a short reaction time of 60 s for the oxidation of substrates, which is much faster than the previously reported ones. The in-depth mechanistic studies by 1H and 31P NMR spectroscopy, transmission electron microscopy, and X-ray photoelectron spectroscopy of the optimized Au–PtNCs-GMP reveal them to be of ultrasmall size with a unique ligand structure, particularly the synergistic effect between the two metals endow the product with a high catalytic performance. Therefore, the present study not only provides a novel bimetallic nanocluster as a good catalyst with highly efficient oxidase mimetics but also opens a way for the development of other oxidase mimetics.
研究领域:
纳米生物学
·
催化化学
·
材料科学
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DOI
JOURNAL OF MATERIALS SCIENCE
·
2017
·Vol. 52
·期 18
·10738-10750
·
IF 2.599
Guo Leilei,Mao Long,Huang Kaixun,Liu Hongmei
In this paper, platinum (Pt)–selenium (Se) nanostructures and Pt nanoparticles were synthesized by a facile one-step chemical reduction route and their catalytic performance was evaluated as oxidase mimic. The results of structure characterization revealed that Pt – Se nanostructures consist of Pt and Se atoms (the Pt/Se atomic molar ratio is approximately 7:3), while Pt nanoparticles consist of pure element Pt. The oxidase-like activity of Pt – Se nanostructures and Pt nanoparticles was evaluated with 3,3′,5,5′-tetramethylbenzidine (TMB) as substrate. The results indicated that Pt – Se nanostructures had a lower Michaelis constant ( K
m ) and higher catalytic constant ( K
cat ) for TMB oxidation than that of Pt nanoparticles,which mean the binary Pt – Se hybrid nanostructures had stronger binding affinity with TMB and higher catalytic activity in comparison with monometallic Pt nanoparticles. The enhanced oxidase-like activity of Pt – Se nanostructures may be due to element Se doped in binary Pt – Se hybrid nanostructures, which can accelerate electron transport and provide excellent chemical stability against catalytic performance degradation during the TMB oxidation reaction. Mercury (II) ions (Hg 2+ ) could inhibit the oxidase mimetic activity of Pt – Se nanostructures and resulted in a color change of the reaction system. Based on this mechanism, a facile colorimetric assay for Hg 2+ was developed with a detection limit as low as 70 nm and a linear range of 0–2.5 μM.
研究领域:
纳米材料
·
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2018
·
IF 5.08
Yunxia Zhuang,Xiaodan Zhang,Qiumeng Chen,Siqi Li,Haiyan Cao,Yuming Huang
Here, we report the synthesis, characterization, and oxidase-like activity of Co 3 O 4 /CuO hollow nanocage (HNC) nanocomposites. The Co 3 O 4 /CuO HNCs were successfully prepared by hydrothermal treatment of ZIF-67 in copper nitrate solution, followed by carbonization in air. The obtained nanocomposites were characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction. The Co 3 O 4 /CuO HNCs possess enhanced oxidase-like activity compared with pure Co 3 O 4 and CuO, and they can catalytically oxidize the 3,3′,5,5′-tetramethylbenzidine (TMB) substrate in the absence of H 2 O 2 to produce an intense blue product. Reactive oxygen species (ROS) measurements indicate that 1 O 2 and O 2 − radicals are the major ROS in catalytic oxidation of TMB by dissolved oxygen in the TMB–Co 3 O 4 /CuO HNC system. We then developed a simple, sensitive, visual, and colorimetric biosensing method for dopamine (DA) based on its inhibiting effect on TMB oxidation. The proposed method allows for DA detection with a limit of detection of 0.027 μM and a dynamic range of 0.05–8 μM. This new colorimetric method was successfully used to detect DA in biological samples. The present work demonstrates a simple strategy to fabricate an efficient oxidase mimic with potential applications in bioanalysis and clinical diagnosis.
研究领域:
材料科学
·
生物传感
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Xiangheng Niu,Mengzhu Wang,Hengjia Zhu,Peng Liu,Jianming Pan,Bangxiang Liu
Monitoring the level of heparin in clinical matrices is significant because of its pivotal role in preventing thrombosis. Compared to traditional single-signal sensors, multi-signal ratiometric detection can provide anti-interference results especially in complicated environments. However, fabricating an easy-to-operation, low-cost and robust sensor for the ratiometric detection of heparin still remains challenging. Here we propose a novel nanosensor for the ratiometric multicolor sensing of heparin with high performance. The sensor is based on the specific electrostatic interaction between the target and a positively charged species generated from nanozyme catalysis. FeMoO 4 nanorods are explored as an oxidase mimic for the first time, showing a high activity at neutral pH to catalyze the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation to blue TMBox with an absorbance at 652 nm. Heparin can induce the rapid aggregation of the produced TMBox intermediate with rich positive charges due to their strong electrostatic interaction, leading to the formation of a purple Heparin-TMBox complex providing a signal at 565 nm. With the increase of heparin, the color changes from blue to indigo and further purple, enabling the multicolor sensing of the target. As a result, ultrasensitive determination of heparin was obtained with a very low detection limit. The fabricated nanosensor could differentiate heparin from complex species with no interferences, and it provided reliable analytical results for heparin in both serum and plasma. With robust performance, low cost and facile fabrication, the sensor holds great potential in monitoring heparin for clinical applications.
研究领域:
生物化学
·
分析化学
·
纳米技术
·
临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 44
·28349-28358
·
IF 4.036
Xin Zhang,Siqin Wang,Jiahao Dao,Jiajing Guo,Yanfang Gao
A novel colorimetric platform based on nano-composites of two-dimensional (2D) molybdenum disulfide nanosheets (MoS2 NSs) and one-dimensional (1D) carbon nanotubes (CNTs), called 2D–1D MoS2-CNT nanozyme, was fabricated for the selective and sensitive determination of hydrogen peroxide (H2O2) in soda water. The MoS2-CNT nanozyme was synthesized through a one-step solvothermal reduction method. The introduced CNTs could effectively prevent the stacking of MoS2 nanosheets (NSs) and not only expanded the interlayer distance of MoS2 NSs from 0.620 nm to 0.710 nm but also improved their specific surface. Under acidic conditions, the as-prepared 2D–1D MoS2-CNT nanozymes could oxidize the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue-oxidized TMB (oxTMB) in the presence of H2O2, resulting in enhanced peroxidase-like (POD-like) activity. The kinetic study showed that MoS2-CNT nanozyme had stronger catalytic activity than natural horseradish peroxidase (HRP). The linear range for H2O2 colorimetric determination was 5.00–500 μmol L−1 with a limit of detection (LOD) of 1.40 μmol L−1. Furthermore, the established determination method was applied to actual samples and the recoveries of H2O2 spiked in soda water were in the range of 92.3–107%, showing feasibility for the analysis of food.
研究领域:
分析化学
·
纳米材料
·
食品分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Shurong Tang,Meili Wang,Zhijun Li,Ping Tong,Qiang Chen,Guangwen Li,Jinghua Chen,Lan Zhang
This work demonstrates the use of quantum dots (QDs) with photocatalytic activity as a sensitive, inexpensive and rapid colorimetric platform for Cu 2+ sensing. Based on the simple thiol compound mediated QDs growing method, CdS QDs can be quickly formed in situ, which possess excellent photocatalytic ability for the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a colored product under light irradiation . Cu 2+ can catalyze the oxidation of sulfhydryl groups in glutathione (GSH) which act as a stabilizer for CdS QDs. In the presence of Cu 2+ , GSH is oxidized and loss the stabilization ability for the growth of CdS QDs, thus resulting in the decrease of the absorbance. Under optimum conditions, as low as 5.3 nM Cu 2+ can be detected. This sensing system is simple, reliable and holds great potential to provide a new general platform for ultrasensitive monitoring of a variety of analytes .
研究领域:
化学
·
材料科学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Chuanmin Ding,Xue Wang,Kaijing Song,Bing Zhang,Junwen Wang,Zhihuan Zhao,Honghong Chang,Wenlong Wei
Herein visible light was utilized as activator to enable colorimetric immunoassay to detect tumor marker based on ternary GO-C 3 N 4 -AgBr heterojunction nanophotocatalyst. Firstly, synthesized photocatalyst GO-C 3 N 4 -AgBr could oxidize TMB due to photogenerated hole and the oxidation ability was enhanced due to ternary heterojunction structure. Secondly, depending on the specific binding of antigen and antibody, the sandwich-type immunocomplex were constructed on the platform of magnetic bead with advantages of convenient cleaning and easy separation. Finally, TMB was added into the ABS buffer solution of immunocomplex. Under visible light radiation, fast change in color was observed within 10 s. Under optimal conditions, there was a linear relation between absorbance and target protein concentration in the range of 0.5 ng mL −1 –25 ng mL −1 with the detection limit of 20 pg mL −1 . Meanwhile, the developed colorimetric immunoassay displayed preferable selectivity, repeatability, and stability.
研究领域:
分析化学
·
材料科学
·
生物医学工程
·
癌症研究
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产品关联
DOI
高影响因子
ANALYST
·
2013
·Vol. 138
·期 16
·4526-4531
·
IF 3.969
Ya Li Liu,Xi Juan Zhao,Xiao Xi Yang,Yuan Fang Li
In this paper, a nanosized porous metal–organic framework, Fe-MIL-88NH2, was facilely prepared with a uniform octahedral shape by the addition of acetic acid, and for the first time was demonstrated to possess intrinsic peroxidase-like activity. Kinetic analysis and electron spin resonance measurements indicated that the catalytic behavior was consistent with typical Michaelis–Menten kinetics and follows a ping-pong mechanism. As a novel peroxidase mimic material, Fe-MIL-88NH2 shows the advantages of high catalytic efficiency, ultrahigh stability and high biocompatibility in aqueous medium compared with natural enzymes and other peroxidase nanomimetics. Here, Fe-MIL-88NH2 was used to quickly catalyze oxidation of the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2 to produce a colored product, which provided a simple, sensitive and selective method for the colorimetric detection of glucose. Glucose could be linearly detected in the range from 2.0 × 10−6 to 3.0 × 10−4 M with a detection limit of 4.8 × 10−7 M, and the color variation for glucose response was also obvious by visual observation at concentrations as low as 2.0 × 10−6 M. More importantly, the colorimetric method could be successfully applied to the determination of glucose in diluted serum samples.
研究领域:
生物分析化学
·
纳米生物技术
·
酶模拟
·
临床检验
·
分子诊断
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）