ORGANIC & BIOMOLECULAR CHEMISTRY
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2018
·Vol. 16
·期 31
·5667-5676
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IF 3.423
Li Huang,Ying-Juan Cao,Xiang-Ying Sun,Bin Liu,Jiang-Shan Shen
Extending the research on 3,3′,5,5′-tetramethylbenzidine (TMB) and its derivatives in analytical chemistry is important, considering that TMB is widely used as an enzyme catalytic substrate. In this work, two TMB derivatives, TMBS and TMBB, were synthesized via a facile and one-step condensation reaction between the –NH2 group of TMB and the –CHO group of salicylaldehyde or benzaldehyde. Because at low pH the two Schiff base compounds can release TMB which can emit strong fluorescence, the probes could show dual-modal signal responses, fluorescence and UV-vis absorption, towards the pH. Practical applications of pH sensing in Chinese rice vinegar and lemon juice samples were successfully demonstrated. On the basis of these findings, a catalytic chromogenic reaction was developed to monitor the pH with the naked eye, too. Furthermore, considering the chemical equilibrium reaction between CO2 and H2O and that glucose oxidase (GOD) can catalyse the dehydrogenation and oxidation reaction of β-D-glucose to produce gluconic acid, both of which can result in lowering the pH values of the two Schiff base systems, highly sensitive and selective dual-modal sensing systems for detecting CO2 and β-D-glucose have also been successfully established. Therefore, the two synthesized TMB derivatives can demonstrate their robust application potential.
研究领域:
分析化学
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生物传感
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酶催化反应
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
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2020
·Vol. 44
·期 30
·12962-12966
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IF 3.288
Jing Sun,Rui Wang,Meng Xia,Shuyun Zhu,Xian-En Zhao
As alternatives to nanozymes, some metal ions have been used in chemical/biochemical sensors owing to their facile acquisition avoiding tedious preparation and purification. Ce4+ has been reported to possess oxidase-like activity. Ascribed to its strong oxidative ability, Ce4+ can trigger the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to its blue product oxTMB without the requirement of H2O2. When ALDS is added, the oxidation ability of Ce4+ can be reduced because of the redox reaction between Ce4+ and ALDS. The absorbance intensity ascribed to oxTMB decreases followed by the fading of the solution. On the basis of such a mechanism, a simple method is proposed for monitoring ALDS in two linear ranges of 0.1–10.0 μM and 10.0–60.0 μM. The detection limit is low to 0.05 μM. Furthermore, this method exhibits great potential for ALDS detection in real biological body fluids and pharmaceutical samples.
研究领域:
分析化学
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药物分析
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DOI
高影响因子
INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
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2019
·
IF 1.267
Kai Sun,Shun-Yao Li,Hui-Ling Chen,Qing-Guo Huang,Youbin Si
In this study, we used a simple and rapid colourimetric reaction for visual sensing of Fe2+ and Pb2+ ions in water by employing nano-MnO2 as a natural oxidase mimic to respectively catalyse ABTS and TMB in citrate-phosphate buffer solution (C-PBS) at 25°C and pH 3.8. It was found that nano-MnO2 possessed highly oxidase-mimicking activity with the Km values of 0.030 and 0.027 toward ABTS and TMB, respectively, indicating TMB had a stronger affinity on nano-MnO2 than ABTS. Interestingly, the presence of 0.01 mmol·L−1 Fe2+/Pb2+ ion was able to significantly down-regulate the activity of MnO2 nanozyme in nano-MnO2-mediated ABTS reaction processes (P < 0.01), which mainly due to the strong adsorption of metal ion toward nano-MnO2 surface via the electrostatic attractions, thus leading to the passivation and inactivation of MnO2 nanozyme catalytic activity. Thereinto, Fe2+ reacted with multivalent manganese by oxidation-reduction, while Pb2+ was specifically adsorbed onto the surface of MnO2 nanozyme and formed complexes. Notably, only Fe2+ ion inhibited the activity of MnO2 nanozyme-TMB with a detection limit as low as 1.0 μmol·L−1. In MnO2 nanozyme-ABTS sensing systems, Fe2+ and Pb2+ ions detection limit of 0.5 and 2.0 μmol·L−1 were, respectively, achieved with a linear response range of 0–0.02 and 0–0.8 mmol·L−1, implying the developed MnO2 nanozyme-ABTS sensor was potentially applicable for the visual determination of Fe2+ and Pb2+ ions in water. In the real water samples, MnO2 nanozyme-ABTS achieved high accuracy (relative errors: 3.4‒10.5%) and recovery (96‒110%) for respective detection of Fe2+ and Pb2+ ions. The simple and rapid MnO2 nanozyme-ABTS sensing systems might provide a practical assay for visual detection of Fe2+ and Pb2+ ions in the environmental water samples.
研究领域:
分析化学
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环境科学
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生物催化
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DOI
高影响因子
JOURNAL OF POROUS MATERIALS
·
2025
·
IF 3.2
Yin Hailiang,Zhou Tongna,Shen Zhonglan,Li Huanhuan,Zhang Guangyan,Guo Lingxiao,Liu Chenguang
A new strategy has been developed for the preparation of ordered mesoporous carbon materials (OMCs) using weak organic acid induction assisted by 1,3,5-Trimethylbenzene (TMB). The type of acid, reaction temperature, TMB addition amount, and salt type all have different significant effects on the microstructure and morphology of carbon materials. In the absence of salt as a pore forming agent and inducing phase separation, OMCs was prepared by acetic acid induction at a reaction temperature of 100 °C and a TMB addition of 1 ml. The specific surface area and pore volume of mesoporous carbon are as high as 927 m 2 /g and 0.78 cm 3 /g, respectively, with a high mesoporous proportion of 72%. The catalyst obtained by loading MoO 2 and NiO nanoparticles on OMCs shows a higher specific surface area and a higher proportion of easily reducible octahedral molybdenum species compared to the catalyst obtained by loading metal on alumina. In the hydrodesulfurization (HDS) experiment of dibenzothiophene, the catalysts showed better HDS catalytic performance and higher hydrogenolysis activity.
研究领域:
材料科学
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催化化学
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纳米材料
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能源与化学工程
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Can Chen,Shiyu Zhang,Chengwu Zhang,Lin Li,Jixin Zhu,Jinhua Liu
Detection of H 2 O 2 is significant for the applications in environmental protection, pharmaceutical industries , food production, and clinical control. Current colorimetric kit of H 2 O 2 based on enzyme or nanomaterials always needs TMB or other peroxidases substrate as coloration species. Herein, we report a simple hydrothermal method to synthesize of TMB dot as the nanosubstrate construction colorimetric kit for highly selective and sensitive detection of H 2 O 2 based on Fenton reaction . Compared with TMB as the substrate, the synthesized TMB dots (TMBDs) show higher reaction activity in the presence of the same low-level Fe 2+ and H 2 O 2 , resulting in highly sensitive sensing H 2 O 2 down to 0.8 nM. Additionally, the obtained TMBDs can well disperse in solution, while generally TMB as substrate needs to be dissolved in organic solvents such as DMF and DMSO. Furthermore, the developed colorimetric kit is applied in detection of Mao-A, due to the reaction between epinephrine and Mao-A producing H 2 O 2 . More importantly, the proposed method has been successfully applied to the analysis of Mao-A in spiked human serum Hence, the TMBDs as the nanosubstrate provide an efficient, fast and sensitive approach for colorimetric kit of H 2 O 2 and Mao-A, promoting more extensive application in early diagnosis of disease and drug discovery.
研究领域:
分析化学
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生物化学
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环境科学
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药学
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临床化学
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DOI
高影响因子
Chemosensors
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2022
·Vol. 10
·期 6
·222
·
IF 4.229
Suqin Liu,Jinjie Wang,Yue Hu,Yunjing Shi,Jingxia Yang,Min Zhang
In the water system, antibiotic pollution significantly impacts the human body and the environment. Therefore, it is essential to quickly identify the types of antibiotics in the system and detect their concentration. It has been reported that many metal ions interact with antibiotics, and some of them can also change the enzyme-like catalytic properties of gold clusters (AuNCs). In the experiments, we found significant differences in the experimental results when different antibiotics and metal ions were placed in a TMB-H2O2system with AuNCs as catalysts. Based on this result, we devised a simple and sensitive colorimetric method for the simultaneous detection of multiple antibiotics using AuNCs-metal ions as the sensor, a multifunctional microplate detector as the detection instrument, and LDA as the analytical method. This method was successfully applied for the identification of antibiotics and the detection of their concentrations in river water.Keywords:gold nanocluster;antibiotic;colorimetric detection;linear discriminant analysis;multiplex detection
研究领域:
环境科学
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分析化学
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纳米技术
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生物技术
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DOI
高影响因子
BIOELECTROCHEMISTRY
·
2025
·
IF 4.5
Guiyin Li,Jing Su,Min Chen,Xiaohong Tan,Lingling Fan,Zhide Zhou,Chong Hu,Jintao Liang
Golgi protein 73 (GP73) has emerged as a critical biomarker for the diagnosis of hepatocellular carcinoma (HCC), and its detection is essential for the effective treatment of this disease. In this study, we developed a colorimetric/electrochemical dual-mode aptasensor for GP73 detection using a hemin-reduced graphene oxide‑manganese oxide (H-rGO-Mn 3 O 4 ) nanozyme and the bifunctional probe, 3,3′,5,5′-tetramethylbenzidine (TMB). The GP73-specific aptamer (Apt) was conjugated to the H-rGO-Mn 3 O 4 nanozyme, which was then immobilized on the surface of a gold nanoparticle-modified screen-printed electrode (Au NPs/SPE) through hybridization with a complementary DNA strand (cDNA) attached to the electrode. TMB served as a dual-signal probe for both colorimetric and electrochemical detection. In the presence of GP73, Apt binds specifically to the target, forming a H-rGO-Mn 3 O 4 -Apt/GP73 complex structure. This binding event causes the nanozymes to detach from the electrode surface, reducing its peroxidase activity while simultaneously restoring the electrochemical signal. Under optimized conditions, the dual-mode aptasensor exhibited excellent linear responses for GP73 concentrations ranging from 0.0001 to 100 ng/mL, with limits of detection (LOD) of 0.1 pg/mL for both the colorimetric and electrochemical modes. The aptasensor was successfully applied to measure GP73 levels in serum samples, yielding highly consistent results. Its performance was further validated by comparison with the standard ELISA method. Compared to single-signal aptasensors, this dual-mode approach not only improves detection sensitivity but also minimizes the risk of false positives or false negatives, making it a more reliable tool for accurate GP73 detection in clinical diagnostics.
研究领域:
肿瘤学
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分子诊断
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生物传感器
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纳米医学
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临床检验
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转化医学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2013
·Vol. 37
·期 7
·2174-2178
·
IF 2.966
Xing Liu,Qi Wang,Yu Zhang,Lichun Zhang,Yingying Su,Yi Lv
In this paper, a novel and simple colorimetric method for the determination of glutathione (GSH) was developed. The BSA–MnO2 NPs possess oxidase-like activity that can catalyze the oxidization of 3,3′,5,5′-tetramethylbenzidine (TMB), and the existence of GSH can cause reduction of oxidized TMB along with a visual color variation. A good linear relationship can be obtained from 0.26 to 26 μM with a limit of detection of 0.1 μM. Furthermore, the good recoveries for serum samples indicated that the present colorimetric methodology was feasible, simple and sensitive, with a promising application in routine analysis of biosamples.
研究领域:
分析化学
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生物化学
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纳米技术
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2024
·
IF 5.5
Qingyong Guo,Rongsheng Xiao,Haifeng Chen,Meishuo Bao,Jingwen Qi,Qian-qian Jia,Wuxiang Zhang
Comprehensive Summary Real-time on-site monitoring of resorcinol (RS) concentrations is crucial for detecting hazardous levels, enabling prompt response measures to mitigate potential environmental and health risks. In this study, we developed an innovative method using CoNi@CN-2 nanozymes to activate peroxymonosulfate (PMS) for oxidizing 3,3',5,5'-tetramethylbenzidine (TMB). Our results show that the formation of Ni 2+ through the oxidation of Ni 0 on the CoNi@CN-2 surface significantly enhances the electron-donating capacity of Co 0 . The catalytic reaction of TMB is mediated by redox active species (SO 4 •− , •O 2 − , •OH and 1 O 2 ). RS drives colorimetry by transferring electrons to the benzene ring and specific nitrogen atoms in ox-TMB, reducing ox-TMB to TMB. Furthermore, the colorimetric assay shows a robust linear correlation between RS concentration and absorbance (Abs), described by Abs = –0.44[RS] + 0.886 (0—200 μmol/L, R 2 = 0.983). Also, we introduce a novel smartphone-integrated autonomous detection software that can analyze RS concentration and grayscale values (GSV), yielding GSV = 0.327[RS] + 63.601 (0—200 μmol/L, R 2 = 0.990) with a detection limit of 5.29 μmol/L. Additionally, excess PMS leads to ROS attacking specific sites in ox-TMB, forming secondary oxidation products. This study has enabled rapid and accurate detection of RS, making a significant contribution to environmental safety and protection.
研究领域:
环境科学
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生物化学
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材料科学
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DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Meng Wang,Jiao Wang,Nan Ma,Shuaibing Yu,Jinming Kong,Xueji Zhang
In this work, a new colorimetric method for the determination of Glutathione (GSH) on the basis of stable free radical 2,2,6,6 − tetramethylpiperidine − 1 − oxyl (TEMPO) oxidation of 3,3′,5,5′−tetramethylbenzizine (TMB) via copper(II) acetylacetonate (Cu(acac) 2 ) catalysis was proposed. TEMPO was catalyzed by Cu(acac) 2 to produce TEMPO + , then TEMPO + oxidized TMB to produce oxidized TMB (ox − TMB). The resulting ox − TMB showed blue and possessed a distinct absorption peak about 650 nm. Whereas, GSH prohibited the generation of ox − TMB through inhibiting TMB oxidation. As compared to the case that GSH was absent, significantly enhanced absorption was determined, and was proportional to GSH amount. On this basis, a qualitative and quantitative detection method of GSH with the naked eye and the microplate reader was achieved. The developed TEMPO − based method achieved GSH biosensing with improved sensitivity in a good specificity − manner. The limit of detection (LOD) was 90 μM via naked eye, and the microplate reader was 4.71 μM. And the stable free radical TEMPO possessed higher stability and lower toxicity than traditional oxidant of H 2 O 2 . Moreover, this TEMPO − based method achieved good results in the detection of GSH in human serums.
研究领域:
分析化学
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生物化学
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生物医学工程
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DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 39
·5007-5016
·
IF 2.378
Xiaofei Qin,Can Chen,Shiyu Zhang,Jixin Zhu,Yongxiang Wang,Jinhua Liu
3,3′,5,5′-Tetramethylbenzidine (TMB) is one of the traditional enzyme substrates that has been widely used in the fields of artificial enzyme mimics and natural enzymes. However, the development of TMB-based probes for fluorescence and colorimetric dual models remains a great challenge. Herein, we developed a facile method to fabricate a colorimetric and fluorescent nanoswitch based on near-infrared fluorescent TMB dots (TMBDs) for the reversible recognition (CFRR) of Fe3+ and Cys. The proposed CFRR nanoswitch was based on an oxidation–reduction mechanism by which the TMBDs are oxidized by Fe3+ to form ensembles and Cys reduces the ensembles to TMBDs. The CFRR nanoswitch exhibited high sensitivity for the colorimetric and fluorescent recognition of Fe3+ with limits of detection (LODs) of 0.17 μM and 7.5 μM and of Cys with LODs of 0.1 μM and 1.5 μM, respectively. The response speed was very fast, within 5 min. Moreover, two output signals, namely near-infrared fluorescent and colorimetric, were exhibited in this CFRR sensor; the recognition was reversible and retained good stability after three cycles. It is noteworthy to mention that the CFRR nanoswitch performed successfully in urine, and molecular logic gate systems were readily established. Therefore, these investigations will shed light on the development of multi-mode output signals for nanoprobes.
研究领域:
分析化学
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纳米技术
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生物传感器
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材料科学
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生物化学
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Tianfei Qi,Ruonan Jia,Linbo Zhang,Guanyu Li,Xuefeng Wei,Jianting Ye,Ruilian Qi,Haiyuan Chen
Self-reporting corrosion protection coatings offer effective means to detect potential failures in advance, allowing for timely maintenance and extending the lifespan of metal materials. However, there currently remains a challenge in developing techniques capable of concurrently monitoring coating damage and metal corrosion throughout a procedure. Herein, N-CDs/Fe 3 O 4 hybrid nanoparticles with high peroxidase-like activity were fabricated through a two-step hydrothermal process which exhibit superior affinity (K m =1.2 mM) towards H 2 O 2 than HRP and effectively turn-on the color of TMB . Combined with the excellent responsiveness towards 8-HQ and the strict linear dependent relationships towards Fe 3+ (ranging from 0.75 to 15.15 μM), a novel colorimetric assay is established to not only turn on the signals to report the appearance of damage, but additionally switch the colors of TMB system to reveal the possible crack propagation and metal corrosion underneath. Moreover, the pre-existing corrosion, characterized by higher Fe 3+ concentration, is successfully indicated according to the color intensity enhancement compared with pristine metal sheet. Notably, such a multifunctional colorimetric assay enables the real-time and visual monitoring coating damage and metal corrosion simply through a Python program integrated into smartphones, serving as a promising strategy for promptly alerting of potential structural failures.
研究领域:
材料科学
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腐蚀科学
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分析化学
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传感器与执行器
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）