RSC Advances
·
2023
·Vol. 13
·期 16
·10503-10507
·
IF 3.9
Chenlong Jiang,Xiangde Lai,Feng Han,Zhijun Gao,Haixia Yang,Xuan Zhao,Huajie Pang,Bin Qiao,Hua Pei,Qiang Wu
Heart failure (HF) is a major public health problem triggered by heart circulation disorders. Early detection and diagnosis are conducive to the prevention and treatment of HF. Hence, it is necessary to establish a simple and sensitive method to monitor the diagnostic biomarkers of HF. The N-terminal B-type natriuretic peptide precursor (NT-proBNP) is acknowledged as a sensitive biomarker. In this study, a visual detection method for NT-proBNP was developed based on the oxidized 3,3′,5,5′-tetramethylbenzidine (TMB2+)-mediated etching of gold nanorods (AuNRs) and double-antibody-sandwich ELISA. The etching color for different amounts of NT-proBNP was obvious and significant differences could be ascertained based on the blue-shift of the longitudinal localized surface plasmon resonance (LLSPR) of the AuNRs. The results could be observed by the naked eye. The constructed system showed a concentration range from 6 to 100 ng mL−1 and a low detection limit of 6 ng mL−1. This method exhibited negligible cross-reactivity toward other proteins, and the recoveries of the samples ranged from 79.99% to 88.99%. These results demonstrated that the established method is suitable for the simple and convenient detection of NT-proBNP.
研究领域:
生物标志物检测
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纳米技术
·
分析化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Xiao-Li Yin,Yun Qing Liu,Hui-Wen Gu,Qi Zhang,Zhao Wei Zhang,Hui Li,Pei-Wu Li,Yu Zhou
A multicolor enzyme-linked immunosorbent sensor was constructed to detect organophosphates pesticides (OPs) on base of TMB 2+ -mediated AuNRs etching. As the etching progresses, a rainbow-like color responses was generated. The naked eye limit of detection (LOD) of the sensing system is 0.25 ng mL −1 . In particular, the longitudinal localized surface plasmon resonances (LSPR) peak shift of AuNRs is linearly correlated with the logarithm of OPs concentration in range of 10 4 to 5 × 10 6 pg mL −1 for parathion-methyl and fenitrothion, 5 × 10 4 to 10 7 pg mL −1 for fenthion, respectively. Compared with reported methods, this sensor displayed satisfactory linear ranges and detection limits. Average recoveries of this method ranged from 73.28% to 98.08% and relative standard deviations (RSDs) were below 8.0% as for food samples analysis without complicated pretreatments. Remarkably, the developed multicolor enzyme-linked immunosorbent sensor is capable of providing accurate and reliable assay for OPs in complex food samples.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Yi Lin,Palanisamy Kannan,Yanbo Zeng,Bin Qiu,Longhua Guo,Zhenyu Lin
In this work, we have synthesized Cu 2+ -modified carbon nitride nanosheets (Cu 2+ -C 3 N 4 ) as peroxidase mimic catalytic substance, which is more active than the horseradish peroxidase enzyme in extreme environments. Gold nanobipyramid (Au NBP) is a good chromogenic substrate for multicolor display because the longitudinal plasmon bands of AuNRs can be easily tuned by adjusting their aspect ratios and used as an excellent indicator for colorimetric detection of immunoassays . The generation of TMB 2+ from sandwich complex (peroxidase-like catalysis) efficiently etches Au NPBs to produce multicolor variations from brown to olive, green, blue, purple, purple, red, pink, and colorless in presence of varied concentrations of neuron specific enolase (NSE). The experimental results show that the colorimetric detection ranging from 312.5 to 20,000 pM with a detection limit of 92.8 pM, which is higher than other multicolor based sensors. The Cu 2+ -C 3 N 4 nanosheets and Au NBPs based colorimetric visual (naked eye) semi-quantitative method as a potential platform towards the detection of important biomolecules in clinical and therapeutic applications. The proposed method is simple, low-cost and enzyme-free to detect NSE for the first time.
研究领域:
纳米技术
·
生物传感器
·
分析化学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 5
·1-9
·
IF 5.705
Yu Xinhui,Lin Yaohui,Wang Xusheng,Xu Liangjun,Wang Zongwen,Fu FengFu
An exonuclease-assisted multicolor aptasensor was developed for the visual detection of ochratoxin A (OTA). It is based on the etching of gold nanorods (AuNRs) mediated by a G-quadruplex-hemin DNAzyme. A DNA sequence (AG4-OTA) was designed that comprises a hemin aptamer and an OTA aptamer. OTA binds to AG4-OTA to form an antiparallel G-quadruplex, which halts its digestion by exonuclease I (Exo I) from the 3′-end of AG4-OTA. Thus, the retained hemin aptamer can bind to hemin to form a G-quadruplex-hemin DNAzyme. This DNAzyme has peroxidase-like activity that catalyzes the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) by H 2 O 2 to produce its diimine derivative (TMB 2+ ) in acidic solution. TMB 2+ can etch the AuNRs by oxidizing Au(0) into Au(I). This results in the generation of rainbow-like colors and provides a multicolor platform for the visual detection of OTA. The assay is based on the use of a single isolated aptamer and possesses obvious advantages such as multi-color visual inspection, relatively high sensitivity and accuracy. It can be used to detect as little as 30 nM concentrations of OTA by visual observation and even 10 nM concentrations by spectrophotometry. The method was successfully applied to the determination of OTA in spiked beer where it gave recoveries of 101–108%, with a relative standard deviation (RSD, n = 5) of <5%.
Schematic of an exonuclease-assisted multicolor bioassay based on the G-quadruplex-hemin DNAzyme-mediated etching of gold nanorods (AuNRs). It enables visual detection of ochratoxin A (OTA) with a detection limit of 30 nM.
研究领域:
分析化学
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食品科学
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纳米技术
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）