JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2020
·
IF 3.004
Qiuyao Du,Yunfeng Zhang,Jifen Wang,Bingjie Liu
An ultra-performance liquid chromatography tandem triple quadrupole compound linear ion trap mass spectrometry (UPLC-Q-TRAP/MS) method was developed and validated for the detection of hypolipidemic drugs in fingerprints. 13 hypolipidemic drugs were well separated by the gradient elution of 0.01% formic acid in water and methanol at a flow rate of 0.4 mL/min within 11 min. The analytes were detected in positive (ESI + ) and negative (ESI - ) modes and scanned using scheduled multiple reaction monitoring-information dependent acquisition-enhanced product ion (SMRM-IDA-EPI) for best selectivity and sensitivity. The calibration curves showed good linearity in the range of 0.050–50.000 ng/patch with coefficients ( r 2 ) higher than 0.9904 for all analytes. Meantime, the LODs and LLOQs were in ranges of 0.001–0.034 and 0.003–0.050 ng/patch. The accuracies, intra-day and inter-day precision ranged from −13.3 to 0.3%, 1.1–10.4% and 3.7–14.5%, respectively. The recoveries ranged from 79.9 to 114.8%, while the absolute and relative matrix effects were in the range of 83.0–107.2% and 2.2–9.7%. By comparing the non-spiked fingerprints from healthy volunteers with the fingerprints obtained from patients, demonstrated that the method was competent for determination and quantitation of hypolipidemic drugs in fingerprints.
研究领域:
分析化学
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药物分析
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质谱分析
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产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
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2019
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IF 6.638
Xiao Yang,Wen Liu,Yifen Shi,Guanghui Xi,Mingshan Wang,Bin Liang,Yakai Feng,Xiangkui Ren,Changcan Shi
It is challenging for traditional hemostatic sponges to meet the clinic demand for both uncontrolled and noncompressible hemorrhage. With the aim to develop a rapid shape recovery material with both active and passive hemostatic performance, a dual-functional hemostatic sponge (TRAP-Sp) with a macroporous structure and good mechanical properties for controlling massive and noncompressible hemorrhage was prepared by chemically immobilizing thrombin-receptor-agonist-peptide (TRAP) onto a starch/polyethylene glycol (PEG) sponge. The TRAP2-Sp1 showed the best hemostatic performance among all samples in both rat artery uncontrollable hemorrhage and liver defect noncompressible hemorrhage models. When analyzing the hemostatic mechanism of TRAP-Sp, the high water absorption capacity of the sponge contributed to absorbing plasma, concentrating blood cells, and enhancing blood coagulation . After absorbing water, the shape-fixed TRAP-Sp with sufficient mechanical strength and high resilience can rapidly expand and apply pressure to the wound. TRAP immobilized on the sponge could activate the adhered platelets in an active pathway. Additionally, evaluation of cytotoxicity, hemolysis, and histology further highlighted the adequate biocompatibility of TRAP-Sp. With excellent hemostatic performance and biosafety, this sponge could be a potential candidate as a topical hemostatic agent for uncontrolled and noncompressible hemorrhage. Statement of Significance There is a need for innovative hemostatic materials for both uncontrolled and noncompressible hemorrhage. This manuscript describes a rapid shape recovery hemostatic sponge with both active and passive hemostatic performances synthesized by foaming technique, cross-linking reaction, and chemical immobilization of thrombin-receptor-agonist-peptide (TRAP). On contact with blood, the shape-fixed sponge can not only rapidly recover its original shape and concentrate platelets and RBCs but also activate the adhered platelets efficiently. The dual-functional sponge has excellent hemostatic efficacy in rat femoral artery hemorrhage and can control noncompressible hemorrhage in penetrating liver wound. Thus, we believe that this sponge could be a potential candidate as a topical hemostatic agent for uncontrolled and noncompressible hemorrhage.
研究领域:
生物材料学
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医学工程
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创伤修复
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Tianhan Zhang,Chengyi Xie,Yong Wu,Wenqing Gao,Chenlu Wang,Keqi Tang,Jiancheng Yu
A compact in-trap single photon ionization (SPI) system, termed discontinuous atmospheric pressure interface (DAPI) ion trap mass spectrometry (ITMS), for the rapid determination of trace volatile organic compounds (VOCs). DAPI enables the maintenance of high analyte pressure in the SPI source while preserving the vacuum. Utilizing vacuum ultraviolet (VUV) lamp as the ion source, we ionize the analyte within an enclosed miniature 3D ion trap for efficient analysis. Experimental findings demonstrate that the system achieves a mass resolution of less than 0.5 atomic mass units for full width at half maxima (FWHM), providing unit resolution. For the response for benzene, the system achieves almost instantaneous response, with rise and fall times of only 2 s, which is a significant advantage compared to the membrane inlet (MI) approach necessary for on-site detection. Additionally, tandem mass spectrometry (MS/MS) with unit m/z isolation window was achieved and resolved dimethyl disulfide and phenol with similar molecular weights. Our results demonstrate that the system provides excellent performance and we anticipate its application in rapidly determining VOCs.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
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2020
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IF 3.347
Haoyi Zheng,Haitao Feng,Wenzhong Zhang,Yantao Han,Wenwen Zhao
With the growth of the aging population, osteoporosis is becoming a global health problem. Ursolic acid (UA) is an active ingredient existed in a variety of foods and nature plants and owns plenty of pharmacological effects especially in treating metabolic disease . Our predication from network pharmacology hinted that UA has potential for ameliorating osteoporosis. Firstly through in vivo experiment, we confirmed that UA administration obviously protected against ovariectomy (OVX)-induced osteoporosis in rats by improving microarchitectural deterioration of trabecular bone ( P < 0.001), decreasing numbers of TRAP positive osteoclast in vertebra ( P < 0.001), as well as decreasing serum osteoclast-specific cytokines release ( P < 0.001). Besides, UA ameliorated kidney damage secondary to OVX-induced osteoporosis by ameliorating glomerular atrophy, decreasing BUN and creatinine levels in OVX rats. In vitro , UA noticeably decreased osteoclastic-special marker proteins c-Fos and NFATc1 expressions ( P < 0.001) in response to RANKL stimulation in macrophagy. Importantly, autophagy pathway was activated in the process of osteoclast differentiation and blocked by UA pretreatment . Furthermore, autophagy inhibitors suppressed osteoclast differentiation ( P < 0.001). Collectively, UA may ameliorate osteoporosis by suppressing osteoclast differentiation mediated by autophagy. Our research provides scientific support for UA treating osteoporosis and offers an optimal dose for daily intake of UA safely to prevent bone diseases.
研究领域:
药理学
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老年医学
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骨骼生物学
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产品关联
DOI
PubMed
高影响因子
Physical Review Research
·
2026
·Vol. 8
·期 3
·033224
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IF 4
Lu Chen,Bingbing Yang,Feng Liu
Secure, repeatable access to information stored in deep electron traps remains the central barrier to realizing the full potential of electron-trapping phosphors in next-generation optical memory. Existing readout methods typically rely on destructive thermal or nonselective optical stimulation, which limits repeated access and compromises data integrity. Here, we introduce photonically engineered trap transfer (PETT), a heat-free, nonvolatile approach enabling selective data extraction from deep traps. A specially designed garnet phosphor, $\mathrm{G}{\mathrm{d}}_{3}\mathrm{G}{\mathrm{a}}_{5}{\mathrm{O}}_{12}$ codoped with $\mathrm{C}{\mathrm{r}}^{3+}$ and $\mathrm{E}{\mathrm{u}}^{3+}$, serves as the storage medium, with $\mathrm{C}{\mathrm{r}}^{3+}$ acting as the luminescent center and $\mathrm{E}{\mathrm{u}}^{3+}$ engineering a hierarchical trap landscape. Data writing is achieved through an up-conversion charging process that embeds data within 0.01 s. In the PETT readout, visible light transfers electrons from deep to shallow traps; subsequent infrared stimulation releases them, producing a detectable luminescence signal. Kinetic modeling of the PETT process describes the time evolution of the shallow-trap population and reveals its characteristic rise-and-decay behavior. This method provides a reliable, selective, and repeatable route to data retrieval and establishes a robust physical framework for secure, multilevel, rewritable optical memory.
✓ 已验证
DOI
高影响因子
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
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2024
·
IF 1.9
Zheng Delu,Cui Chenli,Ye Chengsong,Shao Chen,Zha Xiujing,Xu Ying,Liu Xu,Wang Can
Coenzyme Q10 (CoQ10) is a potent antioxidant that is implicated in the inhibition of osteoclastogenesis, but the underlying mechanism has not been determined. We explored the underlying molecular mechanisms involved in this process. RAW264.7 cells received receptor activator of NF-κB ligand (RANKL) and CoQ10, after which the differentiation and viability of osteoclasts were assessed. After the cells were treated with CoQ10 and/or H2O2 and RANKL, the levels of reactive oxygen species (ROS) and proteins involved in the PI3K/AKT/mTOR and MAPK pathways and autophagy were tested. Moreover, after the cells were pretreated with or without inhibitors of the two pathways or with the mitophagy agonist, the levels of autophagy-related proteins and osteoclast markers were measured. CoQ10 significantly decreased the number of TRAP-positive cells and the level of ROS but had no significant impact on cell viability. The relative phosphorylation levels of PI3K, AKT, mTOR, ERK, and p38 were significantly reduced, but the levels of FOXO3/LC3/Beclin1 were significantly augmented. Moreover, the levels of FOXO3/LC3/Beclin1 were significantly increased by the inhibitors and mitophagy agonist, while the levels of osteoclast markers showed the opposite results. Our data showed that CoQ10 prevented RANKL-induced osteoclastogenesis by promoting autophagy via inactivation of the PI3K/AKT/mTOR and MAPK pathways in RAW264.7 cells.
研究领域:
细胞生物学
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分子生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Bionic Engineering
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2021
·Vol. 18
·期 3
·548-558
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IF 2.682
Jamil Muhammad Imran,Wang Qiongyan,Ali Amjad,Hussain Munir,Aziz Tariq,Zhan Xiaoli,Zhang Qinghua
Ice accumulation is a safety and operational threat in power lines, wind turbines, and transportations. Surfaces having both passive anti-icing and active deicing functionalities are very rare. Here, we report a self-cleaning slippery photothermal trap, which is icephobic passively and deice the surfaces actively by converting sun light to heat at the ice-substrate interface. The photothermal trap consists of three layers: a candle soot layer act as solar radiation absorber, a magnetic iron oxide Fe 3 O 4 nanoparticles layer act as heat spreader for lateral dispersal of sun light, and Room Temperature Vulcanized (RTV) insulation to reduce the transverse heat loss. Upon illumination under microsolar 300, the temperature of the surface increased by 40 °C within 200 s. The heat confinement at the magnetic Fe 3 O 4 nanoparticles layer leads to rapid increase of the surface temperature, ice start to melt and silicone lubricant facilitates the ice removal. The slippery photothermal trap removed the frozen droplet (10 µL) within 40 s upon the illumination of sun light and the frozen droplet was completely converted into water after 7 min illumination of solar light at −20 °C. The developed slippery photothermal trap also melted the fully frost covered layer within 100 s at −20 °C under sunlamp. The average defrosted length (25 mm) was also observed by irradiation of laser light for 45 s. The self-cleaning slippery photothermal coating showed outstanding deicing performance at subzero temperature for long term due to the infusion of silicone oil into the nanostructures and same chemical composition with binder.
研究领域:
材料科学
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能源科学
·
表面工程
✓ 已验证
DOI
高影响因子
BIOSCIENCE REPORTS
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2020
·Vol. 40
·期 7
·
IF 2.942
Huang Ming-Zhi,Zhuang Yong,Ning Xu,Zhang Hao,Shen Zhi-Min,Shang Xian-Wen
Osteoporosis is a metabolic bone disease that is characterized by decreased bone density and strength due to excessive loss of bone protein and mineral content, which can be induced by increased osteoclast activity. Developing agents targeting osteoclast activation is considered to be the most effective method to reverse bone destruction and alleviate the pain caused by osteoporosis. MTT assay was conducted to detect the cell viability after artesunate treatment of RAW264.7 cells. TRACP staining and pit formation assays were performed to examine the TRACP-positive cells and pit-forming activity of osteoclasts. qRT-PCR and Western blot analysis were performed to assess the mRNA and protein expression levels of the osteoclastogenesis-related genes NFATc1, TRAP, and cathepsin k. The protein levels of RANK, p-Akt, p-p38, and p-ERK were examined by Western blotting. Luciferase reporter assay was conducted to determine whether miR-503 targeted RANK directly. Artesunate inhibited TRACP-positive cells and the pit-forming activity of osteoclasts. However, artesunate increased the expression of miR-503. Artesunate suppressed osteoclastogenesis-related gene expression and RANKL-induced activation of MAPKs and the AKT pathway. In addition, miR-503 inhibited RANK expression by directly targeting RANK during osteoclast differentiation. Artesunate inhibited osteoclastogenesis and osteoclast functions in vitro by regulating the miR-503/RANK axis and suppressing the MAPK and AKT pathways, which resulted in decreased expression of osteoclastogenesis-related markers.
研究领域:
药理学
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骨骼生物学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL PHYSICS
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2022
·Vol. 35
·期 2
·369-374
·
IF 1.09
Huang Kai,Zhang Feng,Zhao Feng-Jiao,Yin Hong-Ming
Erbium doped borate glass is widely used in luminescent materials, the luminescence dynamics of erbium doped borate glass is of great significance for optimizing and improving the luminous efficiency. The 2% molar ratio erbium doped borate glass was synthesized by the traditional melt quenching method, and annealed at 260 °C below the borate glass transition temperature. The thermal performance parameters of borate glass undoped and doped with Er 3+ were measured by differential scanning calorimetry with 10 °C/min. The transient emission spectrum and decay kinetics curves were measured for the luminescence mechanism of erbium doped borate. Er 3+ ions have different lifetime when emitted at 556 nm with different excitation wavelengths, the excited state trap may exist in erbium doped borate glass.
研究领域:
材料科学
·
光学
·
发光材料
✓ 已验证
DOI
高影响因子
ANALYST
·
2021
·Vol. 146
·期 21
·6455-6462
·
IF 4.616
Fuxing Xu,Weimin Wang,Xinhua Dai,Xiang Fang,Chuan-Fan Ding
Linear ion trap mass analyzer could improve analytical performance compared to the traditional three-dimensional ion trap. In this study, a systematic investigation of the effect of octopole field on the performance of linear quadrupole ion trap was studied by both theoretical simulations and experiments. An asymmetric semi-circular linear ion trap (AsC-LIT) analyzer was designed and tested based on the theoretical results. The AsC-LIT consists of one pair of larger semi-circular rods with radius Ry and one pair of smaller semi-circular rods with radius Rx (Ry > Rx) in the cross section for introducing the quantitative octopole field. The analytical performances of these simplified AsC-LITs with different amounts of octopole fields were carefully investigated using both theoretical simulations and experimental validation. Experimental results showed that it could have a mass resolution of 1645 at m/z 175 (arginine); furthermore, the low mass cutoff and CID efficiency can be significantly improved when some positive octopole is added. The simulative results indicated that 2.56% octopole component was the best choice for achieving higher analytical performances. The theoretical and experimental studies of this work could certainly enrich the knowledge of a higher order electric field effect on the linear ion trap, furthermore, the simple geometric design and performance characteristics of this new AsC-LIT make it particularly attractive in the development of miniaturized mass spectrometers.
研究领域:
分析化学
·
质谱仪技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Xiaoru Liu,Zhihao Zhao,Baofeng Zhang,Yuexiao Hu,Wenyan Qiao,Yikui Gao,Jing Wang,Ziting Guo,Linglin Zhou,Zhong Lin Wang,Jie Wang
Triboelectric nanogenerator (TENG) offers a novel approach for converting high-entropy mechanical energy into electrical energy, yet achieving high charge density remains critical. Optimizations using dielectrics with high specific capacitance have mitigated air breakdown, but charge loss within dielectrics persists as a limiting factor. Here, based on poly(vinylidene fluoride–trifluoroethylene–chlorofluoroethylene) (P(VDF-TrFE-CFE)) with high specific capacitance, (P(VDF-TrFE-CFE)) composites’ trap density and energy are engineered using high-polarity interfaces from barium titanate (BTO) nanoparticles and dense chain segment stacking induced by electrostatic interaction with polyetherimide (PEI) to enhance charge retention capability. With modified high interfacial traps, an ultrahigh charge density of 9.23 mC m −2 is achieved in external charge excitation (ECE) TENG using 0.2 vol% PEI/P(VDF-TrFE-CFE) film, marking the highest charge density reported for single-unit TENGs. This work provides novel material strategies for high-performance TENGs, paving the way for their large-scale practical applications.
研究领域:
材料科学
·
能源技术
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2017
·
IF 0.795
Xiang QIAN,Qian ZHANG,Xin-Qiong LU,Quan YU,Kai NI,Chao ZHANG,Xiao-Hao WANG
An electrospray/ultraviolet lamp dual-source ion trap mass spectrometer was developed for the rapid detection of gas and liquid samples. A discontinuous atmospheric pressure sampling system was used in the instrument, in which both the electrospray ions and gaseous analytes were sampled and transferred by a pinch valve device. The two ionization sources used here were generally suitable for different kinds of analytes and provide complementary applications. Electrospray was used for the ionization of polar compounds in solution, while the UV ionization source was mainly applied to the analysis of gaseous organic compounds. A variety of samples such as anisole, toluene, 2,4-dimethylaniline, arginine, reserpine and aspartame were employed to test the performance in different working mode of the instrument. The result showed that the two sources was suitable for ionization of different samples, and different types of molecular ions were generated when analyzing 2,4-dimethylaniline. Two ionization sources could be used alternately or simultaneously without interference with the other and the working mode could also be switched to fit the application requirements. Dual-source configuration was an effective way to extend the applications for miniature mass spectrometers. It did not significantly increase the size of the instrument, but provided more versatile analysis to meet the need for the measurement of different types of samples.
研究领域:
分析化学
·
质谱技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）