Tetrastigma hemsleyanum Diels et Gilg, a rare herbal plant native to China, possesses high medicinal value. More researches have focused on the flavones in the tubers of Tetrastigma hemsleyanum whereas polysaccharides attract few attentions. In this study, a purified polysaccharide (TTP-1) with the average molecular weights of 478.33 kDa was extracted from Tetrastigma hemsleyanum and conducted multiple analysis to identify its structural information. In vitro , TTP-1 could suppress the inflammation, cytotoxicity, genotoxicity, mitochondrial dysfunction and oxidative damage induced by LPS in RAW264.7 cells. Meanwhile, TTP-1 could attenuate inflammation via COX-2, iNOS, MAPKs pathways and ameliorate oxidative damage through Nrf2-Keap1, Sirt1-FoxO1 pathways in RAW264.7 cells. In vivo , the inflammation induced by LPS triggered the suppression of survival ratio and growing development, as well as the restrain of athletic ability in Caenorhabditis elegans . The treatment of TTP-1 reversed the situation induced by LPS. In a word, TTP-1 could ameliorate LPS-induced inflammation both in vitro and in vivo .
Jin Li,Donghui Chen,Daquan Zhang,Yizhen Wang,Yinzhe Yu,Lixin Gao,Manhong Huang
The triazole inhibitors of 2-(1-tosyl-1H-1,2,3-triazol-4-yl)-ethanol (TTE) and 2-(1-tosyl-1H-1,2,3-triazol-4-yl)-propan-2-ol (TTP) were synthesized via the click chemistry reaction. Self-assembling technique was used to form self-assembled membrane (SAM) of TTE and TTP on copper surface. The electrochemical measurement results indicate that the TTE and TTP film can strongly suppress copper corrosion in 3 wt.% NaCl solution and the inhibition efficiency of TTE and TTP are 89.4% and 93.1%, respectively. TTP shows better inhibition performance than TTE. Polarization curves show that TTE and TTP predominantly retard the anodic process of the corrosion reaction. The results of quantum chemical calculations and molecule dynamics (MD) simulations reveal that the formation of TTE and TTP SAM on copper surface is mainly achieved by the adsorption of triazole ring and O atoms at horizontal orientation.
危险品化学品经营许可证（带存储）