Treating metabolic dysfunction-associated fatty liver disease (MAFLD) and reducing the occurrence of MAFLD-associated liver cancer remain challenging. Two-dimensional (2D) tantalum carbide (Ta4C3) MXene nanozymes (MXenzymes) exhibit antioxidant and anti-inflammatory activities and have thus attracted considerable attention in the fields of oncology and engineering. However, the potential mechanism of action and bioactive properties of Ta4C3 in MAFLD remain uncertain. In our study, Ta4C3 not only inhibited lipid accumulation and disrupted lipid metabolism in hepatocytes but also reduced cell death caused by fatty acids by decreasing intracellular reactive oxygen species (ROS) levels, which significantly promoted the polarization of M1 macrophages to M2 macrophages by alleviating oxidative stress and further suppressing inflammatory factor expression. In mice fed a methionine–choline-deficient (MCD) diet, Ta4C3 reduced lipid accumulation, the infiltration of inflammatory cells, and liver cell apoptosis by modulating the cellular microenvironment through its anti-inflammatory and antioxidant properties. Therefore, Ta4C3 can be used as a multifunctional bioactive material to alleviate hepatic steatosis and inflammation in individuals with MAFLD/metabolic dysfunction-associated steatohepatitis (MASH) because of its robust antioxidant and anti-inflammatory effects.
Surface-enhanced Raman scattering (SERS) is a novel spectroscopic technique that enables the identification of analytes through analysis of their unique chemical signatures. Its high sensitivity, specificity, and rapid response make it a valuable tool in a range of fields, including biological detection, food safety, and environmental monitoring. However, traditional SERS nanoparticle substrates are susceptible to instability and agglomeration. In this study, Ta4C3 MXene/gold nanostar (AuNSs) hybrids were prepared as SERS substrates. By optimizing the experimental conditions of AuNSs, the optimal preparation method for the Ta4C3 MXene/AuNSs composite structure was identified. The addition of MXene to the Ta4C3 MXene/AuNSs hybrid substrates was found to enhance the sensitivity of the composite for Raman detection, as evaluated using 4-aminothiophenol (PATP) as a Raman molecule. This improvement in sensitivity is attributed to the enhanced electromagnetic properties of the hybrid substrates, which facilitate more efficient charge transfer and enhance the Raman scattering process. The limit of detection (LOD) of Ta4C3 MXene/AuNSs is 10–9 M for PATP and 10–7 M for thiram solutions. In addition, good reproducibility and spatial uniformity were obtained for the Raman signals of Ta4C3 MXene/AuNSs. Furthermore, Ta4C3 MXene/AuNSs were combined with filter paper to create paper-based SERS substrates that could be used for detection. By directly wiping the apple peel and subsequently detecting the thiram residues using Ta4C3 MXene/AuNSs, the level of detection of thiram residues on the peel surface was down to 7.8 ng/cm2.
Atherosclerosis (AS), marked by lipid buildup and chronic inflammation in arteries, leads to major cardiovascular events. Macrophages contribute to AS by engulfing low-density lipoproteins, forming foam cells, and driving inflammation that promotes plaque growth and instability. The emerging piezocatalytic therapy uses piezoelectric materials to generate radicals that target inflammation-related macrophages for AS treatment, but the conventional materials suffer from low radical yield, substantially limiting clinical use. In this study, the construction of piezoelectric BaTiO 3 /Ta 4 C 3 MXene heterostructured nanosheets (BTOMX NSs) is reported for achieving enhanced piezoelectric AS treatment by blocking early atherosclerotic plaque progression. The composite BTOMX NSs feature high electron-hole separation efficiency due to their narrowed bandgap and high surface potential under ultrasound irradiation, enabling more effective radical generation by piezocatalytic effects. Especially, these biocompatible piezoelectric nanosheets accumulate in plaques and are efficiently internalized by macrophages, where they generate radicals under ultrasound stimulation, ultimately triggering macrophage apoptosis and interrupting plaque progression. In ApoE −/− mice, the BTOMX NSs remove lesional macrophages, reduce lipid accumulation, and mitigate inflammation, decreasing plaque burden from 21.42% to 9.04%. Taken together, this work provides a paradigm for enhancing BaTiO 3 -based piezocatalytic performance by heterostructure construction, demonstrating an efficient, noninvasive, and safe therapeutic approach for treating early-stage AS.
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