Comparative Biochemistry and Physiology D-Genomics & Proteomics
·
2024
·
IF 2.2
Shengli Fu,Kun Qian,Xiao Tu,Jie Lu,Tuo Yao,Lingtong Ye,Jianmin Ye
The structure of fish intestines does not have a clear regional division, while the function of the intestines may be related to their structure. Therefore, in this study, the delimitation of intestinal segments in pufferfish ( Takifugu obscurus ) was achieved by morphological analysis. Subsequently, enzyme activity, intestinal microbiota, and gene expression were examined to compare the differences among the pufferfish various segments. According to four morphological parameters: height of mucosa folds (HF), width of mucosa folds (WF), thickness of muscularis (TM), and cross-sectional area (CSA), the pufferfish's intestine was divided into anterior intestine (AI), middle intestine (MI), and posterior intestine (PI). The activity levels of amylase, lipase, and trypsin in the AI and MI were significantly higher than these in the PI. According to the analysis of 16S rDNA, the dominant microbiota at the phylum level in the different segments were Epsilonbacteraeota, Spirochaetes, and Proteobacteria. At the genus level, there were variations observed in the relative abundance of Brevinema, Mycobacterium, Bradyrhizobium, and Microvirga. α diversity analysis revealed that the richness indexes (Ace and Chao1) were the lowest in the MI, while β diversity analysis revealed significant difference in intestinal microbial community composition among the three intestinal segments. Furthermore, RNA-Seq was used to identify differential expression genes (DEGs) and biological pathways among the different intestinal segments. The DEGs between the AI and MI were enriched in pancreatic secretion and protein digestion and absorption, those between AI and PI were involved in ascorbate and aldarate metabolism and glutathione metabolism, and those between MI and PI were involved in steroid biosynthesis, fat digestion and absorption, vitamin digestion and absorption, and glycine, serine and threonine metabolism. In conclusion, the presented results compare and analyze the differences in various intestinal segments of pufferfish, which will be conductive to future exploration of the functions of these different segments.
研究领域:
海洋生物学
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分子生物学
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微生物学
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基因组学
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产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2019
·
IF 3.298
Shengli Fu,Mingmei Ding,Qingjian Liang,Yanjian Yang,Meng Chen,Xiufang Wei,Anli Wang,Shaoan Liao,Jianmin Ye
The immune mechanism elicited in pufferfish ( Takifugu obscurus ) against the invasion of Aeromonas hydrophila is still poorly understood. We examined the spleen of pufferfish at the transcriptome and proteome levels by using Illumina-seq and TMT coupled mass spectrometry after 12 h infection by A. hydrophila , respectively. A total of 2,339 genes (1,512 up-regulated and 827 down-regulated) and 537 (237 up-regulated and 300 down-regulated) proteins were identified. GO and KEGG analyses revealed that the responses to stimulus were the main biological processes , intestinal immune network for IgT production and calcium signaling pathway . Fourteen genes (8 up-regulated and 6 down-regulated) and proteins (5 up-regulated and 9 down-regulated) involved immune responses or signal transduction were validated by qRT-PCR and parallel reaction monitoring to confirm the reliability of the transcriptomic and proteomic analyses, respectively. Moreover, qRT-PCR and flow cytometry were used to detect dynamics of the genes in calcium signaling pathway and changes of concentration of cytoplasm Ca 2+ in spleen cells within a 72 h challenge. This study provides the findings regarding immune response, especially intestinal immune network for IgT production pathway and calcium signaling pathway at the molecular, protein and cellular in pufferfish after infection by A. hydrophila . These results would provide a new insight and molecular targets into the response to pathogenic infection in pufferfish.
研究领域:
免疫学
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分子生物学
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水产养殖
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鱼类病理学
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DOI
PubMed
高影响因子
Antioxidants
·
2026
·Vol. 15
·期 7
·827
·
IF 8.2
Shengli Fu,Kun Qian,Tuo Yao,Jie Lu,Lingtong Ye,Jianmin Ye
Lead (Pb) is a persistent aquatic pollutant that disrupts redox homeostasis in fish. This study investigated hepatic Pb accumulation, reactive oxygen species (ROS) production, antioxidant responses, lipid peroxidation, and transcriptomic alterations in juvenile pufferfish (Takifugu obscurus) exposed to waterborne Pb. Juvenile pufferfish were exposed to 5.98 mg/L waterborne Pb, corresponding to 10% of the 96 h LC50, for 96 h. Liver, blood, and hepatocyte samples were collected at 0, 12, 24, 48, and 96 h, with four biological replicates at each sampling time point. Hepatic Pb accumulation increased over time and reached the highest level at 96 h. ROS levels in blood cells and hepatocytes increased rapidly and peaked at 12 h. Superoxide dismutase (SOD) and catalase (CAT) activities showed early activation followed by late suppression, whereas glutathione peroxidase (GSH-Px) displayed partial adaptive recovery. Malondialdehyde (MDA) content increased progressively and reached approximately 2.8-fold of the control level at 96 h, indicating persistent lipid peroxidation. RNA-seq analysis identified 167, 460, 1398, and 2580 differentially expressed genes at 12, 24, 48, and 96 h, respectively. Enrichment, temporal trend, and weighted gene co-expression analyses indicated that Pb exposure shifted hepatic responses from early redox regulation to later metabolic adaptation, protein processing in the endoplasmic reticulum, proteasome function, and oxidative phosphorylation. qRT-PCR validation of 12 hub genes supported the RNA-seq results. These findings provide integrated biochemical and transcriptomic evidence for oxidative-stress-mediated hepatic toxicity in pufferfish exposed to waterborne Pb.
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DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Yuqian Zheng,Yichen Sun,Yiwen Zhu,Wenli Wang,Imre Blank,Shi Liu,Yin Zhang,Ye Liu,Yuan Liu
Monosodium glutamate (MSG) is a basic representative of umami taste, but it exerts adverse effects as reported in several studies. Enhancing umami perception through aroma-taste interactions is a potentially useful approach as a flavor enhancer to reduce future MSG intake. We identified the aroma compounds in fried Takifugu obscurus liver, then studied the effect of aroma compounds on perceived umami taste using the sensomics approach. A total of 117 volatile compounds were identified from the fried liver. Thereinto, 30 volatile compounds related to five basic tastes were detected by gas chromatography/olfactometry-associated taste analysis. Aroma compounds associated umami, sweet and salty perception in the fried Takifugu obscurus liver showed the potential to enhance umami perception of MSG solutions. Three aldehydes, i.e. heptanal, (Z)-4-heptenal and 2-methylbutanal, significantly enhanced the umami intensity perception of an MSG solution ( p < 0.05). The use of aroma compounds to enhance umami perception is considered as a promising tool, further broadening the research area of umami science for application as flavor enhancers in the food industry. In addition, this study also provides an insight into revealing aroma-taste interaction.
研究领域:
食品科学
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感官科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2018
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IF 2.71
Shengli Fu,Mingmei Ding,Yanjian Yang,Jingrong Kong,Yuan Li,Zheng Guo,Anli Wang,Jianmin Ye
Caspases are a family of proteases involved in many important biological processes, including inflammation, cell differentiation and apoptosis. Among them, caspase-6 plays an important role in the execution phase of the apoptotic death cascade. In this study, the full-length of puffer fish caspase - 6 ( Pfcasp - 6 ) was cloned and characterized, containing an open reading frame of 912 bp, a 5′-UTR of 168 bp and a 3′-UTR of 632 bp with a poly (A) tail. The Pfcasp - 6 encoded a polypeptide of 303 amino acids with an estimated molecular mass of 34.09 kDa and a predicted isoelectric point of 5.35. Phylogenetic analysis indicated that Pfcasp-6 was highly homology to that of other teleostean species. Spatial mRNA expression analysis revealed that the ubiquitous expression of Pfcasp - 6 in all examined tissues, with high expression in skin, followed by gill, intestine, kidney , blood, heart and spleen. After the challenges of high temperatures and Aeromonas hydrophila , the transcript expressions of Pfcasp - 6 were significantly up-regulated in blood and spleen. In addition, cellular localization analysis indicated that the distribution of Pfcasp-6 was in the cytoplasm and nucleus. Overexpression of Pfcasp-6 was able to trigger a significant level of cell death in HeLa cells, suggesting its putative role in cell apoptosis. Taken together, the results of this study suggested that Pfcasp - 6 might be an important component in the caspases cascade, and was likely to get involved in the response to different stress in puffer fish.
研究领域:
分子生物学
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免疫学
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鱼类生理学
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基因表达
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DOI
高影响因子
Food Science and Human Wellness
·
2022
·
IF 8.022
Youyou Li,Shui Jiang,Yiwen Zhu,Wenzheng Shi,Yin Zhang,Yuan Liu
Pufferfish is prone to deterioration due to abundant nutrients and high moisture content. Drying technology can extend the shelf life and enhance the flavor quality of aquatic products. The study investigated the effect of hot air drying (HAD), microwave vacuum drying (MVD) and hot air assisted radio frequency drying (HARFD) on the taste and volatile profiles of Takifugu obscurus . Different drying methods had significant influence on the color, rehydration, 5 ' -nucleotides, free amino acids and volatile components ( P < 0.05). The results showed that HAD and HARFD could promote the flavor of T. obscurus by producing higher equivalent umami concentration (EUC) values, which were about two times of MVD group, and more pronounced pleasant odor according to sensory analysis. HAD is more appropriate for industrial application than HARFD and MVD considering the economic benefits. This study could provide a reference for the industrial application of drying T. obscurus.
研究领域:
食品科学
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水产加工
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产品关联
DOI
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-9
·
IF 5.228
Wang Jun,Zhu Xuexia,Huang Xin,Gu Lei,Chen Yafen,Yang Zhou
Obscure puffer Takifugu obscurus , a species of anadromous fish, experiences several salinity changes in its lifetime. Cadmium (Cd) is a toxic heavy metal that can potentially induce oxidative stress in fish. The present study aimed to detect the combined effects of Cd (0, 5, 10, 20 and 50 mg L −1 ) and salinity (0, 15 and 30 ppt) on juvenile T. obscurus . Results showed the juveniles could survive well under different salinities; however, with Cd exposure, the survival rates significantly decreased at 0 and 30 ppt. At 15 ppt, tolerance to Cd increased. Cd exposure clearly induced oxidative stress and the responses among different tissues were qualitatively similar. Salinity acted as a protective factor which could reduce the reactive oxygen species and malondialdehyde levels. In addition, salinity could enhance the antioxidant defense system, including superoxide dismutase, catalase and glutathione. Na + /K + –ATPase activity significantly decreased under Cd exposure in gill, kidney and intestine. These findings indicated that Cd could moderate the adaptability of juvenile T. obscurus to high salinity and low salinity played a protective role upon Cd exposure. Thus, the role of salinity should be considered when evaluating the effect of heavy metals on anadromous and estuarine fishes.
研究领域:
环境毒理学
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鱼类生理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Peiyun Li,Qianqian Zhou,Yuanming Chu,Weiqing Lan,Jun Mei,Jing Xie
The present study evaluated the effects of chitosan (CH) and sodium alginate (SA) bioactive coatings containing 0.1, 0.2 and 0.3% ε-polylysine (ε-PL) combined with high CO 2 modified atmosphere packaging conditions (MAP, 60% CO 2 /5% O 2 /35% N 2 ) on the quality of cultured pufferfish ( Takifugu obscurus ) at 4 °C for 18 days. Results showed that CH-SA coatings containing ε-PL and high CO 2 MAP treatments could significantly lower total viable count (TVC), Pseudomonas spp., H 2 S-producing bacteria and lactic acid bacteria counts (LAB), and reduce the productions of off-flavor compounds including total volatile basic nitrogen (TVB-N), trimethylamine (TMA) and ATP-related compounds. Volatile compounds were identified and quantified by SPME gas chromatography–mass spectrometer (SPME-GC/MS) for pufferfish during cold storage and the bioactive coating treated pufferfish significantly lowered the relative content of fishy flavor compounds, such as 1-octen-3-ol, hexanal, (E)-2-octenal, octanal and 2,3-butanedione. Further, CH-SA coatings containing ε-PL and high CO 2 MAP treatments could more efficiently retard the water migration by magnetic resonance imaging (MRI) results, and maintained the quality of pufferfish during storage at 4 °C according to organoleptic evaluation results.
研究领域:
食品科学与技术
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水产品保鲜
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活性包装
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食品微生物学
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采后生物学与技术
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DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 18
·6122
·
IF 4.927
Hengli Meng,Shui Jiang,Yin Zhang,Yun Hu,Yuan Liu
Pufferfish is nutritious and delicious, but the tetrodotoxin (TTX) that may exist in its body poses a serious safety hazard. It is important to use scientific and effective methods to detect the TTX in pufferfish, but most of the existing methods require complex pre-treatment steps and have sample lethality. The solid-phase microextraction (SPME) technology can be used for in vivo detection due to its advantages such as no solvent demand, simple operation, and fast detection speed. In this study, the[email protected]SPME fibers were made via a dipping method, and their extraction effect was verified in the TTX aqueous and spiked fish. The established method has good reproducibility, and the limit of detection of TTX in pufferfish was 32 ng·g−1, and the limit of quantitation was 150 ng·g−1, which can meet the detection needs of pufferfish for safe consumption. This method was used to in vivo detect theTakifugu obscurusexposed to the TTX, to determine the content of TTX in the pufferfish muscle. The detection method established in this study can relatively quickly and easily realize the in vivo detection of TTX in the pufferfish, which can provide theoretical support for improvement in the food safety level of the pufferfish.Keywords:tetrodotoxin;Takifugu obscurus;solid-phase microextraction;ultrahigh-performance liquid chromatography–tandem mass spectrometry
研究领域:
食品安全
·
分析化学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Yijia Tang,Jianqiao Xu,Le Chen,Junlang Qiu,Yuan Liu,Gangfeng Ouyang
Fluoroquinolones (FQs) are a group of antimicrobial agents that have been widely used for therapeutic purposes in clinical medicine for human and veterinary diseases, as well as in aquaculture production. Their residues, however, may survive in foods of animal origin, thus causing health risks for human. In this study, a rapid and sensitive method based on in vivo solid-phase microextraction (SPME) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed to detect the residues of five FQs in cultured puffer fish ( Takifugu obscurus ). In vitro fiber evaluation experiment demonstrated that, compared with the thicker polydimethylsiloxane (PDMS) coating (165 µm), the custom-made biocompatible C18-PAN fibers (45 µm) exhibited much higher extraction efficiencies towards FQs (approximately 9–31 times). The custom-made C18-PAN coating also possessed excellent reproducibility in fish muscle with the intra-fiber relative standard deviations (RSDs) ranging from 11.2% to 14.3% (n = 6) and inter-fiber RSDs ranging from 13.1% to 16.1% (n = 6), which was suitable for in vivo sampling. The custom-made SPME fibers were subsequently applied to determine fluoroquinolones in dorsal-epaxial muscle of living puffer fish. The accuracies were verified through the comparison with traditional liquid extraction (LE) method, and the sensitivities were demonstrated to be satisfying with the limits of detection (LODs) ranging from 0.3 ng g −1 to 1.5 ng g −1 . In general, this study presented a convenient and high-efficient method to determine fluoroquinolones in puffer fish by in vivo sampling.
研究领域:
分析化学
·
水产养殖
·
兽医学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Hui Zheng,Haibing Tang,Chunxiang Yang,Jingwen Chen,Li Wang,Qingfeng Dong,Wenzheng Shi,Li Li,Yuan Liu
Active packaging is an innovative and effective way to extend the shelf life of food, but few studies have focused on the effect of its active ingredients on food flavor . This study aimed to develop slow-release polylactic acid/polyhydroxyalkanoates (PLA/PHA) active packaging containing oregano essential oil (OEO) and investigate the effect of active composite packaging on the flavor and quality of pufferfish fillets. The plasticizing effect of OEO increased the elongation at break (EAB) of the films from 23.36% to 65.80%. The adsorption of montmorillonite (MMT) reduces the loss of OEO during processing. The amount of active substance (carvacrol) released from PLA/PHA/OEO/MMT film to pufferfish was 9.70 mg/kg. The pufferfish fillets packed in PLA/PHA/OEO/MMT film showed the slightest difference on the 8th day from the beginning of storage. The slow-release composite films could extend the shelf life of pufferfish fillets by 2–3 days at 4 °C ± 1 °C.
研究领域:
食品科学
·
包装工程
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Peiyun Li,Jun Mei,Jing Xie
In this study, we explored the effectiveness of chitosan (CH) and sodium alginate (SA) bioactive coatings containing increasing concentrations of ε-polylysine (ε-PL) (0.1%, 0.2%, and 0.3%) combined with modified atmosphere packaging (MAP) in 60%CO 2 /5%O 2 /35%N 2 to inhibit myofibril oxidation and degradation of farmed pufferfish during a cold storage period of 18 days. Our research revealed that ε-PL-enriched CH-SA coatings and high CO 2 MAP noticeably delayed myofibril oxidation by inhibiting the formation of carbonyl groups and by keeping higher sulfhydryl content. We also observed that these treatments preserved myofibril Ca 2+ -ATPase activity, protected their organized secondary structure by retaining higher contents of α-helix and β-sheet, and also stabilised tertiary structure during cold storage. The transmission electron microscope (TEM) images showed that the distortion degree of Z-disk and M-line had become deeper and the gap between sarcomeres had expanded gradually when a comparison was carried out between fresh fish and fish at the end of the cold storage period of 18 days. However, the treated samples were still regular in appearance with distinct light I-bands, dark A-bands and Z-disk. Overall, we report that coatings containing ε-PL and high CO 2 MAP treatments appear to protect the myofibrils of farmed pufferfish during cold storage.
研究领域:
食品科学
·
生物化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）