Yixin Sun,Qi Sun,Tiantian Gao,Shumin Ren,Xiaomei Hu
Efficient cellulase production is essential for its application in green bioprocessing, yet the transcriptional regulation of cellulase biosynthesis in Talaromyces sp. remains largely uncharacterized. In this study, we elucidate the regulatory functions of TeXlnR and TeAmyR in Talaromyces endophyticus . Our results demonstrate that TeXlnR, as a potent transcriptional activator, enhances enzyme production by over 33 %. RNA-seq analysis shows that TeXlnR overexpression upregulates 33 cellulase genes and 11 hemicellulase genes, while concurrently repressing the expression of the transcription factor TeAmyR . Further investigation identified TeAmyR as a repressor of cellulase production in T. endophyticus . EMSA and yeast one-hybrid analyses confirmed the specific competitive binding of these two transcription factors to the promoter regions of cellulase genes eg5B and eg12A . TeXlnR both directly activates target gene transcription and indirectly promotes cellulase synthesis by downregulating TeAmyR expression to reduce its competitive binding to promoters. These findings provide direct evidence for competitive regulation by transcription factors in Talaromyces sp. and establish a theoretical foundation for the rational engineering of industrial strains with improved cellulase production.
Yixin Sun,Qi Sun,Yingying Hou,Chunying Li,Xiaomei Hu
Filamentous fungi are key producers of cellulolytic and hemicellulolytic enzymes (CH enzymes), which play pivotal roles in lignocellulosic biomass conversion. Although several Talaromyces sp. have demonstrated the ability to produce CH enzymes, the enzymatic potential and regulatory mechanisms of Talaromyces endophyticus remain poorly understood. This study to systematically investigates the lignocellulose-degrading potential of T . endophyticus , providing new insights into its regulatory networks. The novel strain T . endophyticus NEAU-6 exhibited high CH enzymes activities when cultured on corn stover. Notably, enzymatic hydrolysis of alkali-pretreated corn stover using a crude CH enzyme loading of only 10 FPase/g of substrate resulted in a glucose yield of 70.2 %, highlighting its efficiency at a relatively low enzyme dosage. Structural and compositional characterization confirmed efficient degradation of the biomass. Full-length and comparative transcriptomic analyses uncovered 54 upregulated CH enzyme genes and a regulatory network involving 11 canonical transcription factors and developmental regulators, alongside 15 sugar transporters potentially mediating carbon sensing and uptake. These findings elucidate the regulatory network of lignocellulose degradation in T. endophyticus and highlight its potential as a candidate for further development in enzyme production and biomass conversion.
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