JOURNAL OF INSECT SCIENCE
·
2015
·Vol. 15
·期 1
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IF 1.025
Wang Xuegui,Hao Qiang,Chen Yiqu,Jiang Surong,Yang Qunfang,Li Qing
The major chemical components of four essential oils (EOs) extracted from dry leaves of Citrus limonum , Cymbopogon citratus , Litsea cubeba, and Muristica fragrans were analyzed with gas chromatograph-mass spectrometer and their fumigant, contact, and repellent activities against 10th instar and adults of Tenebrio molitor were also assayed. The results indicated that the major constituents of C. limonum and Cy. citrates were D-limonene (38.22%) and 3,7-dimethyl-6-octenal (26.21%), while which of L. cubeba and M. fragrans were (E)-3, 7-dimethyl-2, 6-octadienal (49.78%) and (E)-cinnamaldehyde (79.31%), respectively. Contact activities of L. cubeba and C. limonum with LC 50 values of 21.2 and 13.9 µg/cm 2 at 48 h and repellence activities (>89.0% repellence indexes) ( P < 0.05) at 12 h on 10th instar were better than those of the other two EOs. Nevertheless, the fumigation activities of L. cubeba on 10th instar and adults (LC 50 = 2.7, 3.7 μl/liter) were stronger than those of C. limonum (LC 50 = 10.9, 12.0 μl/liter) at 96 h and significant (not overlapping confidence intervals). The EOs of L. cubeba and C. limonum have clearly elongated the growth and development of larvae, egg, and slightly shorten pupae and adults of T. molitor compared with the control. The mainly active ingredients of L. cubeba and C. limonum , including D-limonene and β-pinene, were demonstrated to coinhibit the actives of AChE and enhance the toxicities on 10th instar of T. molitor . These results indicate that the EOs of L. cubeba and C. limonum could have great potential as botanical insecticides against T. molitor .
研究领域:
昆虫学
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植物化学
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害虫管理
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PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2022
·
IF 9.136
Ying Shi,Saier Wang,Man Xu,Xinli Yan,Junbiao Huang,Hong-wei Wang
Neonicotinoid pesticides (NEOs) are the most widely used insecticides in the world and pose a serious threat to ecological systems. Thus, the development of an efficient and sustainable technique for their removal is urgently needed. In this study, a novel biochar was prepared by pyrolysis of Tenebrio molitor frass and activated with KOH at 650 (TMFBC-650A), 750 (TMFBC-750A) and 850 °C (TMFBC-850A). Among them, TMFBC-750A showed the best adsorption performance, and the adsorption capacities for thiacloprid (THI), nitenpyram (NIT) and dinotefuran (DIN) were 155.08 mg·g −1 , 195.86 mg·g −1 , 325.81 mg·g −1 , respectively. The isotherms for the adsorption of NIT and THI fit the Langmuir model quite well, while DIN fits the Freundlich model. Moreover, the adsorption of THI, NIT and DIN was well fitted by the pseudofirst-order kinetic model. The physicochemical characterization analysis demonstrated that the adsorption process of NEOs by TMFBC might be mainly controlled by micropore filling, π-π electron donor–acceptor interactions and functional group interactions (H-bonding, covalent bonding and hydrophobic interactions). Additionally, the thermodynamic parameters suggested that NEO adsorption in this work was a spontaneous, endothermic and randomly increasing process. TMFBC also exhibited a high selective adsorption capacity regardless of solution pH and inorganic ion strength concentrations. The regenerated TMFBC (via synergistic ultrasound/ethanol) could sustainably and efficiently absorb NEOs in reuse cycles, suggesting that TMFBC has excellent reusability and stability. These results indicated that TMFBC can be a potential and sustainable adsorbent for neonicotinoid pesticide removal.
研究领域:
环境科学
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农业科学
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Mengyao Liu,Dong Xu,Jun Zhang,Fei Pan,Changhao Liu,Xiangxin Li,Xiaoming Fang,Hualei Chen,Wenli Tian,Wenjun Peng
Tenebrio molitor protein (TMP) is a highly promising alternative protein resource. However, hydrogels formed from TMP alone typically exhibit fragility and brittleness, limiting their development and application. This study investigated the effects of varying chitosan (CS) concentrations on the mechanical properties of TMP hydrogels. We found that CS concentrations between 0.5 and 1.5 % greatly improved the mechanical properties, water-holding capacity (WHC), and rheological properties of the TMP/CS composite gels. Specifically, the incorporation of 1.5 % CS improved the storage modulus of the gel, enhanced the interaction between water and proteins, and yielded a WHC of 53.32 %. Furthermore, the addition of 1.5 % CS altered the secondary structure and spatial conformation of the protein, with a β-folding content of 43.27 %, leading to a significant enhancement of hydrophobic interactions and hydrogen bonding in the composite gel system and the formation of a dense and stable gel network structure. However, the addition of excessive CS (2.0 % ~ 3.0 %) led to phase separation of the gel system, which decreased gel strength and increased the viscosity of composite gels. In conclusion, optimized CS incorporation offers a viable theoretical basis for the development of new TMP-based food formulations.
研究领域:
食品科学
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生物化学
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PubMed
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INDUSTRIAL CROPS AND PRODUCTS
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2022
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IF 6.449
Yufeng Ma,Yu Bei,Meng Zhang,Fei Song,Feilong Hu,Zhimin Kou,Lihong Hu,Yonghong Zhou,Puyou Jia
Woody oil is extracted from the fruits or seeds of woody plants . Preparing woody oil based and biodegradable plasticizer via solvent-free Diels-Alder reaction can reduce plasticizer enrichment and pollution, as well as dependence on petrochemical resources. Herein, woody oil based plasticizer (TOP-Ⅰ and TOP-Ⅱ) were successfully synthesized from renewable woody oil via solvent-free Diels-Alder reaction. Chemical structure of TOP-Ⅰ and TOP-Ⅱ was characterized with 1 H NMR, Biodegradation of TOP-Ⅱ was investigated in active soil and digestive system of Tenebrio molitor . Degradation products were extracted from active soil and Tenebrio molitor feces, the results showed that TOP-Ⅱ experienced biodegradation and formed degradation components with low molecular weight substances. The insect degradation of bio-based plasticizers was first reported and provided an effective way to reduce plasticizer enrichment and pollution. In addition, TOP-Ⅰ and TOP-Ⅱ was used to replace petroleum-based and non-degradable dioctyl phthalate (DOP) in plasticized poly(vinyl chloride) (PVC). The obtained plasticized PVC with TOP-Ⅰ and TOP-Ⅱ showed improved thermal stability, aging resistance, plasticization and solvent extraction resistance, the related mechanism behind these performances were also explored.
研究领域:
化学工程
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环境科学
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生物学
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高影响因子
ENVIRONMENT INTERNATIONAL
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2020
·
IF 7.577
Bo-Yu Peng,Zhibin Chen,Jiabin Chen,Huarong Yu,Xuefei Zhou,Craig S. Criddle,Wei-Min Wu,Yalei Zhang
Tenebrio molitor larvae (Coleoptera: Tenebrionidae) are capable of depolymerizing and biodegrading polystyrene and polyethylene. We tested for biodegradation of Polyvinyl Chloride (PVC) in T. molitor larvae using rigid PVC microplastic powders (MPs) (70–150 μm) with weight-, number-, and size-average molecular weights (M w , M n and M z ) of 143,800, 82,200 and 244,900 Da, respectively, as sole diet at 25 °C. The ingestion rate was 36.62 ± 6.79 mg MPs 100 larvae -1 d -1 during a 16-day period. The egested frass contained about 34.6% of residual PVC polymer, and chlorinated organic carbons. Gel permeation chromatography (GPC) analysis indicated a decrease in the M w , M n and M z by 33.4%, 32.8%, and 36.4%, respectively, demonstrating broad depolymerization. Biodegradation and oxidation of the PVC MPs was supported by the formation of O C and O C functional groups using frontier transform infrared spectroscopy (FTIR) and 1 H nuclear magnetic resonance ( 1 H NMR), and by significant changes in the thermal characteristics using thermo-gravimetric analysis (TGA). Chloride released was counted as about 2.9% of the PVC ingested, indicating limited mineralization of the PVC MPs. T. molitor larvae survived with PVC as sole diet at up to 80% over 5 weeks but did not complete their life cycle with a low survival rate of 39% in three months. With PVC plus co-diet wheat bran (1:5, w/w), they completed growth and pupation as same as bran only in 91 days. Suppression of gut microbes with the antibiotic gentamicin severely inhibited PVC depolymerization, indicating that the PVC depolymerization/biodegradation was gut microbe-dependent. Significant population shifts and clustering in the gut microbiome and unique OTUs were observed after PVC MPs consumption. The results indicated that T. molitor larvae are capable of performing broad depolymerization/biodegradation but limited mineralization of PVC MPs.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2026
·
IF 11.3
Hui Zhang,Chao-Fan Yin,Xin Song,Ning-Yi Zhou,Ying Xu
Polyvinyl chloride (PVC), a persistent and ecotoxic plastic, poses severe environmental risks. In this study, two bacterial strains, Acinetobacter sp. PVC-6A and Bacillus sp. PVC-6B, were isolated from Tenebrio molitor gut. These two isolates were able to utilize PVC (M n 28 kDa, M w 64 kDa, M z 110 kDa) as the sole carbon source, causing respective 2.55% and 2.51% weight loss of additive-free PVC films (1 cm × 1 cm) after 35 days of incubation at 30°C and pH 7.2. PVC degradation by both isolates was confirmed by multimodal characterization, including SEM, AFM, ATR-FTIR, and WCA. GC-MS analysis detected a novel chlorinated intermediate, 1-chlorohexadecane, along with multiple medium- and long-chain fatty acids, suggesting a partial dechlorination process during PVC depolymerization. This chlorinated intermediate supported the growth of strain PVC-6A and released nearly stoichiometric chloride ions, demonstrating further metabolic dechlorination. This pathway, which proceeds via multi-step dechlorination, is distinct from previously proposed PVC degradation mechanisms. Genomic and transcriptomic analyses identified a catalase-peroxidase (CAT) and two laccases (LAC1, LAC2) as key enzymes involved in PVC degradation. Recombinant enzyme treatments caused PVC depolymerization and dechlorination, with LAC1 exhibiting the highest activity, yielding a 13.1% reduction in molecular weight and 77% surface dichlorination ( 35 Cl - ). Molecular docking with AlphaFold3-predicted structures elucidated enzyme-plastic interactions. By revealing a novel PVC biodegradation pathway, identifying depolymerizing enzymes, and providing preliminary insights into enzyme-substrate binding, this study advances our comprehension of PVC biodegradation. It thereby establishes a theoretical foundation for developing bio-enzymatic remediation strategies against this persistent chlorine-releasing pollutant.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Lei He,Shan-Shan Yang,Jie Ding,Zhi-Li He,Ji-Wei Pang,De-Feng Xing,Lei Zhao,He-Shan Zheng,Nan-Qi Ren,Wei-Min Wu
Polyethylene terephthalate (PET) is a mass-produced fossil-based plastic polymer that contributes to catastrophic levels of plastic pollution . Here we demonstrated that Tenebrio molitor (mealworms) was capable of rapidly biodegrading two commercial PET resins (microplastics) with respective weight-average molecular weight (M w ) of 39.33 and 29.43 kDa and crystallinity of 22.8 ± 3.06% and 18 ± 2.25%, resulting in an average mass reduction of 71.03% and 73.28% after passage of their digestive tract, and respective decrease by 9.22% and 11.36% in M w of residual PET polymer in egested frass. Sequencing of 16 S rRNA gene amplicons of gut microbial communities showed that dominant bacterial genera were enriched and associated with PET degradation. Also, PICRUSt prediction exhibited that oxidases (monooxygenases and dioxygenases), hydrolases (cutinase, carboxylesterase and chitinase), and PET metabolic enzymes, and chemotaxis related functions were up-regulated in the PET-fed larvae. Additionally, metabolite analyses revealed that PET uptake caused alterations of stress response and plastic degradation related pathways, and lipid metabolism pathways in the T. molitor larvae could be reprogrammed when the larvae fed on PET. This study provides new insights into gut microbial community adaptation to PET diet under nutritional stress (especially nitrogen deficiency) and its contribution to PET degradation.
研究领域:
环境科学
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微生物学
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生物降解
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昆虫学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2019
·
IF 7.149
Bo-Yu Peng,Yiming Su,Zhibin Chen,Jiabin Chen,Xuefei Zhou,Mark Eric Benbow,Craig S. Criddle,Wei-Min Wu,Yalei Zhang
Yellow mealworms (larvae of Tenebrio molitor, Coleoptera: Tenebrionidae) have been proven to be capable of biodegrading polystyrene (PS) products. Using four geographic sources, we found that dark mealworms (larvae of Tenebrio obscurus) ate PS as well. We subsequently tested T. obscurus from Shandong, China for PS degradation capability. Our results demonstrated the ability for PS degradation within the gut of T. obscurus at greater rates than T. molitor. With expanded PS foam as the sole diet, the specific PS consumption rates for T. obscurus and T. molitor at similar sizes (2.0 cm, 62–64 mg per larva) were 32.44 ± 0.51 and 24.30 ± 1.34 mg 100 larvae–1 d–1, respectively. After 31 days, the molecular weight (Mn) of residual PS in frass (excrement) of T. obscurus decreased by 26.03%, remarkably higher than that of T. molitor (11.67%). Fourier transform infrared spectroscopy (FTIR) indicated formation of functional groups of intermediates and chemical modification. Thermo gravimetric analysis (TGA) suggested that T. obscurus larvae degraded PS effectively based on the proportion of PS residue. Co-fed corn flour to T. obscurus and wheat bran to T. molitor increased total PS consumption by 11.6% and 15.2%, respectively. Antibiotic gentamicin almost completely inhibited PS depolymerization. High-throughput sequencing revealed significant shifts in the gut microbial community in both Tenebrio species that were associated with the PS diet and PS biodegradation, with changes in three predominant families (Enterobacteriaceae, Spiroplasmataceae, and Enterococcaceae). The results indicate that PS biodegradability may be ubiquitous within the Tenebrio genus which could provide a bioresource for plastic waste biodegradation.
研究领域:
环境科学
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微生物学
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昆虫学
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PubMed
高影响因子
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
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2016
·
IF 0.824
Yan-chen Wang,Peng Li,De-fu Chi
To utilize plant volatiles in the monitoring and biological control of Tenebrio molitor L., electroantennogramography (EAG), Y-tube olfactometry and wind tunnel bioassays were conducted to test the electrophysiological and behavioral responses of T. molitor to nine individual volatiles and five essential oils (compounded volatiles). The aim of this study was to select effective plant compounds that could be used in the development of semi-chemical based push–pull methods for the control of this harmful stored product pest. T. molitor displayed strong EAG responses to cis-3-hexenol, isoeugenol, α-pinene, turpentine oil, eucalyptus oil and peppermint essential oil. Y-tube olfactometry assays indicated that peppermint essential oil showed greater luring effects on adult T. molitor than the control. Cis-3-hexenol elicited strong repellent effects on adult T. molitor , and wind tunnel assays indicated that cis-3-hexenol showed strong repellent effects on adult T. molitor . Peppermint essential oil showed greater luring effects than the control. These results provide a basis to further the development of T. molitor luring and repellent agents.
研究领域:
昆虫学
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化学生态学
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DOI
高影响因子
Heliyon
·
2020
·
IF 0
Tao Feng,Zhongshan Hu,Yanzun Tong,Lingyun Yao,Haining Zhuang,Xiao Zhu,Shiqing Song,Jun Lu
Chicken liver is a main protein source to prepare attractant for dog food. However, animal proteins are costly. Seeking high quality and low-cost protein sources has been a goal for the industry. Mushroom Lentinus edodes (L. edodes) and Mealworm Tenebrio molitor (T. molitor) are novel protein sources, showing high potential as raw material of attractants. In this paper, chicken liver, L. edodes, and T. molitor were used as three different protein sources to prepare attractants. Their palatability to dogs were then compared. Firstly, the enzymatic hydrolysis process of three proteins was optimized, with a degree of hydrolysis of 54.82%, 36.10% and 30.14% for chicken liver, L. edodes , and T. molitor respectively. Secondly, volatile compounds of three attractants were identified by HS-SPME/GC-MS and SDE/GC-MS. Using OAV and PLRS method, it was found that bis(2-methyl-3-furyl) disulfide, indole, methional, 2-(methyl thio) phenol, γ -butyrolacton, furfuryl alcohol, acetic acid and isovaleraldehyde were the key components. Although both T. molitor and L. edodes attractant showed less palatability than that of chicken liver, they could be readily improved via adding key palatable volatile compounds. The ingestion rate of dog food with attractant showed a similar trend and was higher than that of food without attractant.
研究领域:
食品科学
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动物营养学
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DOI
PubMed
Applied Food Research
·
2025
·
IF 6.2
Qin Liu,Jian Li,Jiale Liu,Yishui Cai,Luo Yang,Tajie Sangzhu,Bo Ye,Manjun Yang
The rising cost of soybean meal has posed challenges for tilapia ( Oreochromis niloticus ) farming. This study explores Tenebrio molitor larval meal (TLM) as a sustainable alternative, leveraging its ability to convert agro-food wastes into high-quality feed. By replacing 15%, 30%, and 60% of soybean meal with TLM in tilapia diets, we observed significant improvements in meat quality. Aquatic extracts of tilapia dorsal muscle exhibited enhanced antioxidant activity, evidenced by increased 2, 20-azinobis(3-ethylben-zothiazoline 6-sulfonate) (ABTS), 2, 2- diphenyl-1-picrylhydrazyl (DPPH), and hydroxyl radical scavenging rates, alongside elevated ferric reducing antioxidant potential (FRAP) values. Metabolomic analysis revealed upregulated amino acid and fatty acid abundances in the liver, with glutathione metabolism playing a pivotal role. Key metabolic pathways, including fatty acid synthesis via fatty acid synthase (FAS), carboxylase (ACC), and acetyl Co-A and protein biosynthesis via glutamine synthetase (GS) and glutamate synthase (GOGAT), were activated. Additionally, the tricarboxylic acid (TCA) cycle was enhanced, further supporting biosynthesis. These findings elucidate the metabolomic mechanisms by which TLM improves tilapia meat quality, offering a viable strategy for sustainable aquaculture feed formulation.
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高影响因子
Foods
·
2024
·Vol. 13
·期 17
·2817
·
IF 4.7
Xiu Wang,Xiangxiang Ni,Chaoyi Duan,Ruixi Li,Xiao’e Jiang,Mingfeng Xu,Rongrong Yu
The objective of this study was to explore the impacts of various ultrasonic powers (0, 300, 500, 700, and 900 W) on the structure and functional attributes of the myofibrillar protein (MP) ofTenebrio molitor. As the ultrasonic intensity escalated, the extraction efficiency and yield of the MP rose, while the particle size and turbidity decreased correspondingly. The reduction in sulfhydryl group content and the increase in carbonyl group content both suggested that ultrasonic treatment promoted the oxidation of the MP to a certain extent, which was conducive to the formation of a denser and more stable gel network structure. This was also affirmed by SEM images. Additionally, the findings of intrinsic fluorescence and FTIR indicated that high-intensity ultrasound significantly altered the secondary structure of the protein. The unfolding of the MP exposed more amino acid residues, the α-helix decreased, and the β-helix improved, thereby resulting in a looser and more flexible conformation. Along with the structural alteration, the surface hydrophobicity and emulsification properties were also significantly enhanced. Besides that, SDS–PAGE demonstrated that the MP ofT. molitorwas primarily composed of myosin heavy chain (MHC), actin, myosin light chain (MLC), paramyosin, and tropomyosin. The aforementioned results confirmed that ultrasonic treatment could, to a certain extent, enhance the structure and function of mealworm MP, thereby providing a theoretical reference for the utilization of edible insect proteins in the future, deep-processing proteins produced byT. molitor, and the development of new technologies.
研究领域:
食品科学
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蛋白质化学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）