Three novel quinoline alkaloids, tetradiunitiside A ( 1 ), tetradiunitiside B ( 2 ), and glycohaplopine-6- O -α- l -rhamnopyranoside ( 3 ), along with eight known ones ( 4–11 ), were isolated from the fruits of Tetradium glabrifolium . Their structures were inferred by IR, 1D NMR and 2D NMR, and HR–ESI–MS spectra. All the isolated compounds were evaluated for their antibacterial activities. Compounds 1–11 had potential antibacterial activities against Bacillus gasoformans , Bacillus subtilis , Pseudomonas solanacearum , and Pseudomonas aeruginosa , with minimum inhibitory concentration (MIC) values ranging from 100 to 3.125 µg/mL. Among them, compound 6 showed the most significant antibacterial activity against four strains of bacteria. These findings elucidated the pharmacological substance basis for the antibacterial activity of T. glabrifolium to some extent. Graphical Three novel quinoline alkaloids together with eight known ones were isolated from the fruits of Tetradium glabrifolium . The antibacterial activity of compounds against four bacteria strains were screened, and 11 compounds exhibited significant antibacterial activity.
Bi Wang,Pirui Li,Jingjing Yang,Xuhong Yong,Min Yin,Yu Chen,Xu Feng,Qizhi Wang
Phytophthora capsici is a notorious pathogen capable of infecting various host plants and causing serious threats to agriculture worldwide. Tetradium glabrifolium fruit essential oil (TFO) is widely utilized in traditional Chinese medicine treatment, but its antimicrobial activity is unknown. In this study, we first investigated the inhibitory efficacy of TFO against P. capsici and found TFO strongly inhibited the development of phytophthora blight on pepper by in vivo inoculation assay. Second, we found TFO could significantly suppress vegetative and reproductive growth of P. capsici in vitro . Microscope observations indicated that TFO destroyed the mycelial morphology and cellular ultrastructure of P. capsici. Additionally, TFO could damage the cell membrane integrity of the pathogen, leading to cellular leakages of intercellular contents of P. capsici . Third, TFO could enhance the activities of defence-related enzymes in pepper fruits during pathogen infection. Finally, we identified the chemical composition of TFO by GC-MS analysis and demonstrated three abundant components ( D -limonene, β -elemene, and 2-tridecanone) contributed to the antifungal activity of TFO. Together, out results revealed that TFO had outstanding inhibitory efficacy against P. capsici , and uncovered its potential antifungal mechanism. This study would propose the application of TFO as an efficient alternative approach for controlling phytophthora blight.
危险品化学品经营许可证（带存储）