TALANTA
·
2015
·
IF 3.545
Yexuan Mao,Lanlan Yu,Ran Yang,Chuanguo Ma,Ling-bo Qu,Peter de B. Harrington
The formation of highly ordered fibrils for the human islet amyloid polypeptide (hIAPP) is considered as one of the precipitating factors of type 2 diabetes mellitus. In this study, an emerging new approach microscale thermophoresis and conventional ThT fluorescence assay were utilized to investigate the aggregation behavior of hIAPP 11–20 , giving a new insight of the solvent effect on the aggregation of hIAPP 11–20 . hIAPP 11–20 displayed different aggregation behaviors in various buffers, revealing that hIAPP 11–20 not only self-aggregates but also binds to solvent components. hIAPP 11–20 had a higher binding affinity for Tris than other selected buffers because multiple hydrogen bonds form, resulting in weaker self-aggregation of hIAPP 11–20 at the early stage of aggregation and prolonging the fibril formation process. hIAPP 11–20 displayed similar self-aggregation in both HEPES and pure water. Negatively charged phosphate ions in the PBS solution ‘neutralize’ the charges carried by hIAPP 11–20 itself to some extent, causing rapid aggregation of hIAPP 11–20 , and leading to a shorter fibrillation process of hIAPP 11–20 . These results revealed that solvents contribute to the aggregation of hIAPP 11–20 and demonstrated the affect of solvents on the activity of biomolecules. Additionally, as a new technique, microscale thermophoresis offers a powerful and promising approach to study the early stages of aggregation of peptides or proteins.
研究领域:
生物化学
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分子生物学
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糖尿病研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2022
·Vol. 147
·期 8
·1631-1640
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IF 5.227
Yunkang Ma,Chenhui Wu,Wenjie Yang,Zhigang Gao,Lihua Chen
Mycoplasma ovipneumoniae (MO), a disease-causing pathogen with some of the highest levels of morbidity and mortality, can spread silently at the herd level. A novel alternative nanoprobe for MO was prepared using porous metal–organic frameworks (MOFs) as the scaffold and hairpin DNA with specific sequences of MO and G4 segments as the probe. This preparation was based on the strong fluorescence emission by ThT (thioflavin T) in the limitation of G-quadruplexes with a cavity structure. The use of MOFs effectively limited the folding behavior of G4 as a part of the probe to improve the defect of the strong background signal caused by the free-state G4Probe in a buffer. The results from the selectivity experiment showed that only a trace amount of the target with lower ΔG could be the “key” to the highly efficient triggering of the release behavior of the G4Probe from MOFs and the subsequent change in the fluorescence behavior of ThT. The DNA targets could be determined by observing the change in the signal. More importantly, the probe showed a low detection limit and a good linear correlation between the concentration of target DNA ranging from 10−10 M to 10−6 M not only in buffer but also in natural complex media. Moreover, the operation involved in the whole strategy was simple and the total cost was low. These findings demonstrated the value of the probe in further clinical diagnosis. This study reports the successful construction of a ΔG-sensitive sensor for MO for the first time.
研究领域:
生物技术
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微生物学
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分析化学
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纳米技术
✓ 已验证
DOI
PubMed
高影响因子
International Journal of Nanomedicine
·
2024
·
IF 6.6
Mai Zhang,Yan Li,Chunli Han,Shiying Chu,Peng Yu,Wenbo Cheng
Background Inhibition of amyloid β protein fragment (Aβ) aggregation is considered to be one of the most effective strategies for the treatment of Alzheimer’s disease. (-)-Epigallocatechin-3-gallate (EGCG) has been found to be effective in this regard; however, owing to its low bioavailability, nanodelivery is recommended for practical applications. Compared to chemical reduction methods, biosynthesis avoids possible biotoxicity and cumbersome preparation processes.Materials and Methods The interaction between EGCG and Aβ42 was simulated by molecular docking, and green tea-conjugated gold nanoparticles (GT-Au NPs) and EGCG-Au NPs were synthesized using EGCG-enriched green tea and EGCG solutions, respectively. Surface active molecules of the particles were identified and analyzed using various liquid chromatography-tandem triple quadrupole mass spectrometry methods. ThT fluorescence assay, circular dichroism, and TEM were used to investigate the effect of synthesized particles on the inhibition of Aβ42 aggregation.Results EGCG as well as apigenin, quercetin, baicalin, and glutathione were identified as capping ligands stabilized on the surface of GT-Au NPs. They more or less inhibited Aβ42 aggregation or promoted fibril disaggregation, with EGCG being the most effective, which bound to Aβ42 through hydrogen bonding, hydrophobic interactions, etc. resulting in 39.86% and 88.50% inhibition of aggregation and disaggregation effects, respectively. EGCG-Au NPs were not as effective as free EGCG, whereas multiple thiols and polyphenols in green tea accelerated and optimized heavy metal detoxification. The synthesized GT-Au NPs conferred the efficacy of diverse ligands to the particles, with inhibition of aggregation and disaggregation effects of 54.69% and 88.75%, respectively, while increasing the yield, enhancing water solubility, and decreasing cost.Conclusion Biosynthesis of nanoparticles using green tea is a promising simple and economical drug-carrying approach to confer multiple pharmacophore molecules to Au NPs. This could be used to design new drug candidates to treat Alzheimer’s disease.
研究领域:
纳米技术
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药理学
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神经科学
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材料科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Liang Zhang,Jie Zhan,Xiayu Liu,Boyuan Guan,Peichao Zhang,Deshang Zhang,Wenjun Wang,Guanchen Liu,Hui Zhang,Donghong Liu
The functionalized modification of proteins from more sustainable resources is urgently required. Herein, the fibrillization of oilseed proteins derived from different sources was conducted, and the conformation-fibrillization relationship was further elaborated by systematically comparing the physicochemical and functional properties of amyloid fibrils. The results indicated that fibrillization-induced alterations in particle size and ζ-potential, ThT fluorescence intensity, surface hydrophobicity, flexibility and rheological property differed among proteins due to their diverse proportions of secondary structure and subunit compositions. Compared with soy proteins, walnut proteins were more likely to be hydrolyzed into peptides with a smaller particle size (108.6 nm) and fibrillated at a higher conversion rate (65.4%). The sesame proteins featured by a higher level of β-sheet exhibited increased surface hydrophobicity and flexibility of fibrils, resulting in higher viscosity due to the formation of cluster-like fibrillar aggregates. The rapeseed protein fibrils derived from cruciferin and napin demonstrated the lower digestibility (20.51%) as they displayed strong resistance against acid and proteolytic digestion during in-vitro digestion. Furthermore, among four oilseed proteins, short-flexible fibrils from sesame and walnut proteins could adhere firmly to oil/water interface, maintaining a gel-like structure of HIPEs with excellent printing accuracy and high self-supporting capacity.
研究领域:
食品科学
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蛋白质化学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）