Chemistry Central Journal
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2017
·Vol. 11
·期 1
·1-15
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IF 2.442
Zhang Xiaoyan,Sun Wenbo,Yang Zhou,Liang Yan,Zhou Wei,Tang Lei
Background
Toddalia asiatica root bark as an effective hemostatic natural medicine or Chinese materia medica was applied in China for long history, its complex drug action mechanisms and unclear substance basis have been constraining the development of this drug.Results An intelligentized strategy by LC-ESI Q-TOF MSE was presented in this study for rapid identification of hemostatic chemical constituents from this natural medicine. Chromatographic separation was performed on a C18 column (150 mm × 2.1 mm, 1.8 μm), the MSE data in both negative and positive ion modes were acquired to record the high-accuracy MS and MS/MS data of all precursor ions. To reduce the false positive identifications, structural confirmation was conducted by comparison with the isolated reference standards (tR and MS, MS/MS data) or matching with natural product databases. Bioassay-guided fractionation of the extract of T. asiatica root bark was also carried out.Conclusion sAs a consequence, 31 natural compounds in T. asiatica root bark got putatively characterized. There were four main coumarins, isopimpinellin (Cp.23), pimpinellin (Cp.24), coumurrayin (Cp.30) and phellopterin (Cp.34) isolated and identified from T. asiatica root bark. The present study provided candidate strategy that helps to effectively identify the primary natural compounds of TCM or other complex natural medicines, and then promote development and application of natural medicines and their medicinal resources.
研究领域:
药理学
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中药学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCES IN POLYMER TECHNOLOGY
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2020
·
IF 1.539
Yunlong Liu,Jie Guo,Zhuobing Xiao,Dazhao Peng,Ke Song
Separation and purification of active components from biomass by inorganic materials during the pretreatment process of hydrothermal conversion are studied in this work. The batch experiment results show that an initial solution pH of 6 favors chelerythrine adsorption, and the optimum adsorbent dosage is 2.0 g. The adsorption mechanism of ZSM-5 for chelerythrine is investigated by adsorption kinetics, isotherm adsorption models, and thermodynamics analysis. The results show that the kinetics data fit the pseudo-second-order model well (R2 = 0.9991), and the intraparticle diffusion model has 3 diffusion stages, preliminarily indicating that chemisorption plays a major role in the adsorption process, and the sorption mechanism includes intraparticle, external, and boundary diffusion. The adsorption isotherms agree well with the Langmuir model, indicating the occurrence of monolayer molecular adsorption during the adsorption process. Meanwhile, the maximum adsorption capacity is 2.327, 2.072, and 1.877 mg/g at different temperatures (288 K, 298 K, and 308 K), respectively. The thermodynamic data demonstrate that the adsorption process is exothermic and spontaneous in nature. These observed results clearly confirm that ZSM-5 has potential superior properties for the enrichment and purification of alkaloids during the pretreatment of biomass.
研究领域:
生物化学
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环境科学
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材料科学
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Chenxi Wu,Xiaojin Tian,Ziyan Song,Wang Rui,Hezhao Bai,Hailin Li,Yidan Wang,Wenzhi Yang,Fei Tian
Designing adsorbents with high sensitivity, superior adsorption capacity, molecular specificity, and batch-to-batch reproducibility remains a significant challenge in separation science. For the first time, we synthesized a functionalized magnetic metal-organic framework (Fe₃O₄@SiO₂@MIL-101@CLCS), to adsorb magnoflorine, hesperidin, nitidine chloride, and chelerythrine from Toddalia asiatica (L.) Lam via electrostatic interactions and π-π stacking. In order to systematically analyze the target components, this study established a magnetic solid-phase extraction coupled with high-performance liquid chromatography-diode array detection (MSPE-HPLC-DAD) method, and comprehensively optimized the extraction and elution conditions. After optimization, the adsorption rates of Fe₃O₄@SiO₂@MIL-101@CLCS on the above four active ingredients were ranged from 52.52% to 82.43%, and the elution rates were all higher than 96.40%. The adsorbent retained good adsorption capacity after 5 reuse cycles and had excellent recyclability. The method demonstrated excellent linearity (r² ≥ 0.999), with a limit of detection (LODs) of 0.17–0.92 μg/mL and a limit of quantification (LOQs) of 0.30–1.65 μg/mL, confirming high accuracy and precision. This approach not only enhances analytical efficiency in traditional Chinese medicine studies but also expands the potential of magnetic solid-phase extraction for natural product research.
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产品关联
DOI
PubMed
高影响因子
FITOTERAPIA
·
2024
·
IF 2.5
Songqin Yang,Mao Zhao,Yuhan Feng,Xia Zhang,Qiuhong Li,Wenwen Jiang,Daoping Wang
Toddalia asiatica (L.) Lam. (TA) is a traditional folk medicine of ethnic minorities in the southwest of China. It is widely used in the treatment of dispersing blood stasis and activating blood. However, the effective substance and pharmacological mechanism have not been fully elucidated. The zebrafish larvae were treated with Phenylhydrazine (PHZ) to establish a thrombus model, and the staining intensity of zebrafish red blood cells was analyzed. The antithrombotic activity of TA was verified for the first time, and it was found that the inhibition rate of TA on thrombosis was up to 60.85 %. The chemical ingredients of TA were collected by combining UPLC-HRMS analysis and the literature research. Network pharmacology revealed that six key targets were obtained, which including TNF, AKT1, EGFR, PTGS2, PPARG, and IFNG. It showed that the PI3K-Akt pathway was a core signaling pathway. Coagulation factor III(TF), playing an important role in the process of hemostasis and thrombosis, which ranks high in the PPI network. Moreover, the results of molecular docking showed that the active components had a strong binding force with TF, which indicated that TF might be the key target of TA in treating thrombosis. In vitro experiments showed that TA could inhibit TNF-α-induced high expression of TF in EA.hy926 cells. In addition, TA could inhibit TNF-α-activated expression of Akt, IκBα and P65 protein phosphorylation in PI3K-Akt pathway. The results showed that TA had antithrombotic activity and exerted an antithrombotic effect by inhibiting the expression of TF through the PI3K-Akt-NF-κB signaling pathway.
研究领域:
药理学
·
分子生物学
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传统中药
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斑马鱼研究
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网络药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）