FOOD CHEMISTRY
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2022
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IF 9.231
Xinyu Ni,Xuyan Li,Guojing Ran,Jiali Chen,Xinwei Jiang,Jianxia Sun,Weibin Bai
This study aimed to determine whether Trachinotus ovatus and Pampus argenteus could be differentiated by multi-element composition or stable isotope ratio analysis of δ 13 C and δ 15 N. The multi-element analysis values of a total of 60 Pampus argenteus samples from three sites and 60 Trachinotus ovatus samples from four sites around China were determined, and the other 60 samples of Pampus argenteus and 51 samples of Trachinotus ovatus were tested by principal component analysis (PCA) to estimate the accuracy of origin identification. The results showed that the cross-validation accuracy rate is 92.2% for the Pampus argenteus and 98.3% for the Trachinotus ovatus . Stable isotope analysis of δ 13 C and δ 15 N also can distinguish Trachinotus ovatus from different geographical origins. These results showed that the usefulness of multi-element and stable isotope analysis as indicators for authenticating the geographical origin of two pomfrets in China.
研究领域:
食品科学
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海洋生物学
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分析化学
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生态学
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溯源技术
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水产养殖
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高影响因子
Food Bioscience
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2022
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IF 5.318
Mengfan Hou,Huan Xiang,Xiao Hu,Shengjun Chen,Yanyan Wu,Jiachao Xu,Xianqing Yang
This study aimed to isolate and identify the novel potential Xanthine oxidase (XOD) inhibitory peptides derived from Trachinotus ovatus hydrolysate (TOH) (hydrolysis by neutral protease), and investigate their structure-function relationship. Initially, TOH was separated to four fractions (F1–F4) by gel filtration chromatography and F4 was chosen with higher XOD inhibitory rate (85.16% ± 0.566, 25 mg/mL) and lower molecular weight (95.73%, <1000 Da). F4 was further characterized by reversed-phase high performance liquid chromatography (RP-HPLC) coupled with Nano-HPLC-MS/MS and four peptides (FPAW, LLPW, WLLP and FHLP) were identified with IC 50 value of 3.81 ± 0.18 mM, 4.17 ± 0.12 mM, 43.06 ± 0.73 mM, and over 50.00 mM respectively. It can be concluded that Trp-containing peptides especially located in C-terminal could inhibit XOD effectively. Moreover, the XOD-inhibiting actions of these peptides involved π-π stacking (Phe914 and Phe1009 in XOD), hydrogen bonding via molecular docking . Lineweaver-Burk equation showed the inhibition type of FPAW was a mixed inhibition. Circular dichroism (CD) spectra indicated FPAW could combine and change the secondary structure of XOD. Therefore, two peptides of FPAW and LLPW might have potential to be used to alleviate hyperuricemia in the future.
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高影响因子
JOURNAL OF THE WORLD AQUACULTURE SOCIETY
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2022
·Vol. 53
·期 6
·1084-1100
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IF 3.402
Wei-Wei Mao,Zi-Chen Zhao,Gui-Hua Li,Wei-Liang Guo,Ze-Hao Ding,Shi-Feng Wang,Yun Sun,Zhen-Jie Cao,Jian-Long Li,Yong-Can Zhou
Cryptocaryon irritans , one of the most devastating holotrichous ciliated protozoans, causes massive economic losses in the pompano, Trachinotus ovatus , mariculture industries. Considering the many advantages of phytochemicals, such as low cost, low residuals, and low pollution, they can be a good alternative for the prevention and control of C. irritans infestations. In this study, 14 phytochemicals were evaluated for their antiparasitic activity against the theront of C. irritans , and nine showed significant antiparasitic activity. Nerolidol was the most promising compound, causing 100% mortality of theronts with 30-min exposure at a concentration of 10.0 μl/L. The half-maximal inhibitory concentrations ( IC 50 s) of nerolidol on C. irritans theronts, protomonts, and tomonts were 2.1, 13.3, and 7.1 μl/L, respectively. Abnormal morphologies of the theronts, trophonts, and tomonts were observed after exposure to 10.0, 30.0, and 10.0 μl/L nerolidol solution for 15 min, 30 min, and 480 min, respectively. A diet supplemented with 2.3 ml/kg of nerolidol reduced the number of trophonts on the gills of pompano by 95.7% decrease compared with the control groups and the theront chemotaxis to pompano mucus and sera, while increased the survival rate of infected pompanos by 24.44%, and had no significant effect on pompano hindgut and serum myeloperoxidase. These results suggest that nerolidol is a new potential drug for controlling fish cryptocaryonosis.
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INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2023
·Vol. 58
·期 9
·4683-4696
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IF 3.3
Weiqing Lan,Bingjie Zhang,Shucheng Liu,Yuqing Sun,Jing Xie
Summary Chitosan-grafted caffeic acid (CS-g-CA) was successfully prepared using carbodiimide-based chemical coupling method. The results of UV–vis and FT-MIR showed that caffeic acid (CA) was successfully grafted onto chitosan (CS). The grafting degree of CA in CS determined by Folin–Ciocalteu scheme was up to 91.26 ± 2.58 mg g −1 . Compared with CS solution, CS-g-CA solution has lower apparent viscosity and thermal stability. The results of SEM demonstrated that the grafting treatment changed the microstructure of CS. The grafting of the CA on CS enhanced its antioxidant activity. It can be seen that the clearance rate of DPPH (82.52% ± 0.7%), ABTS + free radicals (88.21% ± 0.26%), T-AOC (94.91% ± 0.21%) and FRAP (13.51 ± 0.52 mmol L −1 FeSO 4 ) of CS-g-CA was similar to ascorbic acid at 2.0 mg mL −1 . The results of antibacterial experiments demonstrated that CS-g-CA had better antibacterial ability in vitro , and the diameter of inhibition zone of 20.0 mg mL −1 CS-g-CA against Pseudomonas fluorescens and Staphylococcus saprophyticus was 16.88 ± 0.05 mm and 19.17 ± 0.09 mm, respectively, and it was significantly higher than CS (11.25 ± 0.04 mm and 13.09 ± 0.07 mm). The texture and freshness of Control group and CS rapidly declines post-mortem. CS-g-CA treatment could effectively retard the deterioration of texture and keep the better quality of Pompanos ( Trachinotus ovatus ). This study indicates that grafting CA onto CS alters the biological activity and physicochemical properties of CS.
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高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2023
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IF 6
Ahtisham Ali,Jinfang Wang,Wei Jiang,Shuai Wei,Qinxiu Sun,Qiuyu Xia,Zefu Wang,Zongyuan Han,Yang Liu,Shucheng Liu
Differential metabolites and potential biomarkers of hybrid grouper and golden pompano muscles were identified in this study. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed to evaluate liquid chromatography-mass spectrometry (LC-MS) metabolomic analysis. Among the metabolites, 1678 co-contained differential metabolites in positive modes and 1445 co-contained differential metabolites in negative modes were investigated. Sixteen most differential metabolites were determined as potential biomarkers in different muscle groups. The top 10 KEGG-enriched pathways were identified. The sensory characteristics of groups were highest in aroma and sulfur-organic compounds in both fishes. Most organic acid contents in hybrid grouper were significantly higher than those of golden pompano. This present study provides a baseline for a better understanding of potential metabolites involved in muscle quality and taste as well as could provide a piece of further knowledge to identify the significant role of key biomarkers in fish muscles.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Jieqian Zhou,Qinxiu Sun,Zongyuan Han,Naiyong Xiao,Zefu Wang,Ouyang Zheng,Shucheng Liu
Refrigerator freezing (RF), immersion freezing (IF) and static magnetic field-assisted immersion freezing (MIF) were employed on golden pompano ( Trachinotus ovatus ), and the structure and functional properties of golden pompano myofibrillar proteins (MPs) during frozen storage were investigated. The results showed that the α -helix content, endogenous fluorescence intensity and total sulfhydryl content of the MIF samples during frozen storage were higher than those of the other frozen samples ( P < 0.05), indicating that MIF treatment inhibited the disordered protein structure changes and oxidation in protein caused by frozen storage. The emulsification properties showed that the MIF group formed and maintained the finest emulsion droplets during frozen storage. The low-field nuclear magnetic resonance (LF-NMR) results revealed that the immobilized water content ( P 21 ) of the MIF myofibrillar protein gel was 1.25 % and 2.48 % higher than that of the IF and RF samples after 180 days of frozen storage. These findings demonstrated that the MIF treatment contributed to the full swelling of MPs to form a dense gel structure, efficiently trapping water. In general, MIF could suppress the structural changes in myofibrillar proteins and the decrease the emulsification and gel properties of golden pompano caused by frozen storage.
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DOI
PubMed
高影响因子
Frontiers in Immunology
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2023
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IF 7.3
Du Hehe,Zhou Yongcan,Du Xiangyu,Zhang Panpan,Cao Zhenjie,Sun Yun
Introduction: Insulin-like growth factor binding protein 5 (IGFBP5) exerts an essential biological role in many processes, including apoptosis, cellular differentiation, growth, and immune responses. However, compared to mammalians, our knowledge of IGFBP5 in teleosts remains limited.Methods: In this study, TroIGFBP5b, an IGFBP5 homologue from golden pompano (Trachinotus ovatus) was identified. Quantitative real-time PCR (qRT-PCR) was used to check its mRNA expression level in healthy condition and after stimulation. In vivo overexpression and RNAi knockdown method were performed to evaluate the antibacterial profile. We constructed a mutant in which HBM was deleted to better understand the mechanism of its role in antibacterial immunity. Subcellular localization and nuclear translocation were verified by immunoblotting. Further, proliferation of head kidney lymphocytes (HKLs) and phagocytic activity of head kidney macrophages (HKMs) were detected through CCK-8 assay and flow cytometry. Immunofluorescence microscopy assay (IFA) and dual luciferase reporter (DLR) assay were used to evaluate the activity in nuclear factor-κB (NF-κβ) pathway.Results: The TroIGFBP5b mRNA expression level was upregulated after bacterial stimulation. In vivo, TroIGFBP5b overexpression significantly improved the antibacterial immunity of fish. In contrast, TroIGFBP5b knockdown significantly decreased this ability. Subcellular localization results showed that TroIGFBP5b and TroIGFBP5b-δHBM were both present in the cytoplasm of GPS cells. After stimulation, TroIGFBP5b-δHBM lost the ability to transfer from the cytoplasm to the nucleus. In addition, rTroIGFBP5b promoted the proliferation of HKLs and phagocytosis of HKMs, whereas rTroIGFBP5b-δHBM, suppressed these facilitation effects. Moreover, the in vivo antibacterial ability of TroIGFBP5b was suppressed and the effects of promoting expression of proinflammatory cytokines in immune tissues were nearly lost after HBM deletion. Furthermore, TroIGFBP5b induced NF-κβ promoter activity and promoted nuclear translocation of p65, while these effects were inhibited when the HBM was deleted.Discussion: Taken together, our results suggest that TroIGFBP5b plays an important role in golden pompano antibacterial immunity and activation of the NF-κβ signalling pathway, providing the first evidence that the HBM of TroIGFBP5b plays a critical role in these processes in teleosts.
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DOI
PubMed
高影响因子
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