INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Xue Wang,Zhuo Zhang,Huan Lei,Chenhui Zhu,Rongzhan Fu,Xiaoxuan Ma,Zhiguang Duan,Daidi Fan
Ulcerative colitis (UC) is a prevalent inflammatory bowel disease, and conventional treatments, such as anti-inflammatory medications and surgery, often prove inadequate due to frequent recurrences and various complications. To alleviate patient suffering, there is an urgent need for a therapeutic system that specifically delivers drugs to the colon for wound healing, inflammation relief, and restoration of microbial homeostasis. In this paper, we developed a Tremella polysaccharide drug-carrying hydrogel that adheres to the inflamed colonic mucosa, forming an effective artificial barrier and releasing the drug in situ to restore local immune and microbial balance. The hydrogel backbone was synthesized through the chemical cross-linking of Tremella polysaccharide with 1,4-butanediol diglycidyl ether in an alkaline environment. During this process, Soluplus® and TPGS-encapsulated ginsenoside compound K adhered to the hydrogel backbone due to electrostatic attraction. The enhanced adhesion following cross-linking enables the hydrogel to stably attach to the inflamed colonic mucosa, releasing mixed micelles that improve drug penetration and absorption by inhibiting the cellular efflux protein P-glycoprotein. This mechanism promotes local immune recovery and eliminates harmful intestinal flora, providing significant relief from UC symptoms. This natural polysaccharide-based hydrogel represents a highly effective oral treatment for UC.
研究领域:
医学
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生物材料学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Stem Cell Research & Therapy
·
2025
·Vol. 16
·期 1
·1-20
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IF 7.3
Liu Yonghao,Zhou Heng,Pang Pengcheng,Liu Ye,Situ Jieying,Wang Junnan,He Yan,Ye Qingsong
Background Mesenchymal stem cell exosomes are the most extensively researched type of Extracellular vesicles (EVs) that offer novel avenues for hair regeneration. However, their use in the field of hair regeneration was limited by their poor production of exosomes. It has been discovered that intracellular vesicles (IVs), which are produced at a higher rate than exosomes, play a comparable biological purpose. As a result, we developed HTMI-MN, a microneedle that uses tremella, a type of Tremella polysaccharide (TPS), and hyaluronic acid (HA) as matrix materials. It has magnetic intracellular vesicles (Mag-IVs), which work in concert to treat androgenic alopecia (AGA) and encourage hair growth.Methods After characterization of the Mag-IVs, we evaluated the effect on angiogenesis by scratch assay, angiogenesis assay, Western Blot and ELISA assay. In addition, we tested the protective effect of Human hair papillary cells (HHDPCs) by CCK-8 method, Western Blot and flow cytometry. Finally, the effects of tremella polysaccharide on M1/M2 polarization of macrophages were detected by fluorescence staining, Western Blot and flow cytometry. AGA model was established in vivo by DHT, and treatment was given by microneedle injection.Results Our study found that Mag-IVs have greater power to promote angiogenesis and protect HHDPCs from apoptosis compared to other vesicles. Besides, tremella polysaccharide can make the transformation of macrophages to anti-inflammatory phenotype. Taken together, in vivo experiments showed that hair regeneration was faster in HTMI-MN-treated mice.Conclusion These results indicate that Mag-IVs and tremella polysaccharide can synergistically improve the hair microenvironment, which has a promising future for AGA treatment.
研究领域:
再生医学
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干细胞研究
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生物材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Packaging and Shelf Life
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2023
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IF 8
Xiaofang Zhao,Zhaolian Han,Shanshan Zhang,Gulijiannaiti Abuduaini,Xin Wen,Tingting Liu,Zhiqiang Cheng
This study aimed to investigate the physicochemical properties, antioxidant and antibacterial activities of Tremella polysaccharides combined with soybean protein isolate (TFP-SPI), natural antibacterial agent ε-polylysine (PL) and polyvinyl alcohol (PVA) hybrid membrane and its applicability in the blueberry preservation. In this study, TFP-SPI was successfully prepared, and it was mixed with PL and PVA to prepare nanofiber membranes. FTIR results showed that TFP-SPI had hydrogen bond interaction. The physicochemical properties showed that the incorporation of TFP-SPI improved the hydrophilicity and mechanical properties of the membrane, providing the best water vapor barrier performance. In vitro antioxidant experiments showed that PVA/TFP-SPI/PL membranes possessed antioxidant capacity . In vitro antibacterial experiments showed that PVA/TFP-SPI/PL membranes had antibacterial activity, especially against Staphylococcus aureus . The results of fruit preservation showed that PVA/TFP-SPI/PL membrane could effectively extend the shelf life of blueberries and maintain good quality. Therefore, PVA/TFP-SPI/PL membrane is a potential active packaging material with promising application prospects.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）