CARBOHYDRATE POLYMERS
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2021
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IF 9.381
Lei Ma,Zhiying Liu,Zhijian Kong,Mengmeng Wang,Tuo Li,Han Zhu,Qun Wan,Dongyang Liu,Qirong Shen
Lytic polysaccharide monooxygenases (LPMOs) are attracting much attention for their potential application in biodegradation. However, there are limited studies on the characterization of the AA11 family. Here, a novel AA11 family protein, Tg AA11, from Trichoderma guizhouense NJAU 4742 was characterized, and the isothermal titration calorimetry (ITC) analysis results showed that it exhibited tight binding capacity for copper ions with a K d value of 4.83 ± 0.79 μM. The MALDI-TOF-MS analysis results indicated that Tg AA11 could act on β-chitin to form C1 oxidation products, and some deacetylated chitooligosaccharides. In addition, the degradation of α-chitin and β-chitin by a chitinolytic enzyme Sg -chi was substantially increased in the presence of Tg AA11 by 39.9 % and 288.2 %, respectively. Furthermore, the active site residues predicted showed that His61 and Tyr142 might be critical for the active site residues of the Tg AA11 protein. This study will contribute to the understanding of the function of AA11 LPMOs in the degradation of chitin.
研究领域:
微生物学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Microbial Cell Factories
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2022
·Vol. 21
·期 1
·1-14
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IF 6.352
Xia Yanwei,Wang Jingfan,Guo Chuanxu,Xu Huanhuan,Wang Wei,Yang Mingzhu,Shen Qirong,Zhang Ruifu,Miao Youzhi
Background Filamentous fungi are highly efficient at deconstructing plant biomass by secreting a variety of enzymes, but the complex enzymatic regulation underlying this process is not conserved and remains unclear.Results In this study, cellulases and xylanases could specifically respond to Avicel- and xylan-induction, respectively, in lignocellulose-degrading strain Trichoderma guizhouense NJAU4742, however, the differentially regulated cellulases and xylanases were both under the absolute control of the same TgXyr1-mediated pathway. Further analysis showed that Avicel could specifically induce cellulase expression, which supported the existence of an unknown specific regulator of cellulases in strain NJAU4742. The xylanase secretion is very complex, GH10 endoxylanases could only be induced by Avicel, while, other major xylanases were significantly induced by both Avicel and xylan. For GH10 xylanases, an unknown specific regulator was also deduced to exist. Meanwhile, the post-transcriptional inhibition was subsequently suggested to stop the Avicel-induced xylanases secretion, which explained the specifically high xylanase activities when induced by xylan in strain NJAU4742. Additionally, an economical strategy used by strain NJAU4742 was proposed to sense the environmental lignocellulose under the carbon starvation condition, that only slightly activating 4 lignocellulose-degrading genes before largely secreting all 33 TgXyr1-controlled lignocellulases if confirming the existence of lignocellulose components.Conclusion sThis study, aiming to explore the unknown mechanisms of plant biomass-degrading enzymes regulation through the combined omics analysis, will open directions for in-depth understanding the complex carbon utilization in filamentous fungi.
研究领域:
分子生物学
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微生物学
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生物技术
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酶学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL MICROBIOLOGY
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2020
·Vol. 22
·期 11
·4808-4824
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IF 4.933
Guan Pang,Tingting Sun,Zhenzhong Yu,Tao Yuan,Wei Liu,Hong Zhu,Qi Gao,Dongqing Yang,Christian P. Kubicek,Jian Zhang,Qirong Shen
Summary Filamentous fungi are known as producers of a large array of diverse secondary metabolites (SMs) that aid in securing their environmental niche. Here, we demonstrated that the SMs have an additional role in fungal defence against other fungi: Trichoderma guizhouense , a mycoparasite, is able to antagonize Fusarium oxysporum f. sp. cubense race 4 (Foc4) by forming aerial hyphae that kill the host with hydrogen peroxide. At the same time, a gene cluster comprising two polyketide synthases is strongly expressed. Using functional genetics, we characterized this cluster and identified its products as azaphilones (termed as trigazaphilones). The trigazaphilones were found lacking of antifungal toxicity but exhibited high radical scavenging activities. The antioxidant property of trigazaphilones was in vivo functional under various tested conditions of oxidative stress. Thus, we conclude that the biosynthesis of trigazaphilones serves as a complementary antioxidant mechanism and defends T. guizhouense against the hydrogen peroxide that it produces to combat other fungi like Foc4.
研究领域:
真菌学
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生物化学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）