CHEMISTRY OF NATURAL COMPOUNDS
·
2021
·Vol. 57
·期 2
·312-314
·
IF 0.809
Qin Chan,Hu Zhibo,Xiong Yahong,Chen Min,Li Chunyuan,Ding Weijia
A new sesquiterpene derivative, ethyl hydroheptelidate ( 1 ), was isolated from the mangrove endophytic fungus Trichoderma harzianum R1. Its structure was elucidated by comprehensive spectroscopic analyses. This compound showed high antifungal activity against two plant pathogenic fungi, Fusarium oxysporum and Colletotrichum musae , and moderate to weak antibacterial activity against three types of avian pathogenic Escherichia coli: O8, O78, and O101.
研究领域:
微生物学
·
天然产物化学
·
植物病理学
✓ 已验证
DOI
高影响因子
CHINESE SCIENCE BULLETIN
·
2013
·Vol. 58
·期 26
·3266-3273
·
IF 1.319
Wu Qiong,Bai LinQuan,Liu WeiCheng,Li YingYing,Lu CaiGe,Li YaQian,Lin ZhenYa,Wang Meng,Xue ChunSheng,Chen Jie
Streptomyces lydicus A01 resists many plant pathogens (including Fusarium spp.) by producing the antifungal agent natamycin, which binds to the ergosterol of fungal cell membranes and inhibits the growth of pathogens. Trichoderma harzianum CECT2413 is a widely-distributed soil fungus that antagonizes several plant fungal pathogens (including Fusarium spp.) by producing chitinase and degrading chitin, a major component of the fungal cell wall. This study attempted to enhance the biocontrol effect of S. lydicus A01 on Fusarium spp. by transforming the chitinase gene of Trichoderma . Chitinase and natamycin could act synergistically on both the cell walls and cell membranes of pathogens. The 33-kD chitinase-encoding gene ( chit33 ) was cloned and conjugal-transformed from T. harzianum CECT2413 to S. lydicus A01, and then confirmed via polymerase chain reaction (PCR) assays. Subsequent analyses using the 3,5-dinitrosalicylic acid (DNS) method and ultraviolet spectrophotometry showed that compared with its wild type strain (WT), the S. lydicus A01 conjugal transformant (CT) with chit33 gene exhibited substantially higher chitinase activity and natamycin production. The resistance of S. lydicus A01- chit33 CT and WT to four Fusaria in crops and vegetables was tested via the cup-plate method. Compared with the WT, the conjugal transformant of S. lydicus A01 with chit33 gene from T. harzianum CECT2413 showed greatly increased biocontrol effect on fusarium disease. This study would be beneficial to the development of high-quality antifungal bio-agents for agricultural applications via the synergy between the previously non-existent and pre-existing functions achieved through heterogeneous gene transformation.
研究领域:
微生物学
·
植物病理学
·
分子生物学
✓ 已验证
DOI
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2025
·
IF 5.7
Qiaoran Ma,Guobing Tian,Shengchao Yang,Junwen Chen,Wei Fan,Ping Zhao,Yan Wang,Jiamin Liu,Yonglin Liu,Shuhui Zi,Shuran He
Planting vegetation under forests in agroforestry systems fosters sustainable agricultural development. However, Limited availability of biostimulants for agroforestry and unclear mechanisms of plant growth promotion. This study synthesized and evaluated a novel biostimulant, nanosilicon-based vermicompost leachate (NSVCL), using Panax quinquefolius L. as the research plant species for forest cultivation. Trichoderma harzianum (TH) was chosen to represent a biostimulant with broad-spectrum properties, and its application effects were compared with NSVCL. The regulatory effects of both on the physiological characteristics and rhizosphere soil microenvironment of P. quinquefolius were investigated, with untreated plants serving as controls. Compared to the control, NSVCL and TH increased the dry weight of P. quinquefolius roots 129.33 % and 23.50 %, respectively. NSVCL was applied more effectively than TH. Additionally, NSVCL markedly improved leaf anatomical traits, including palisade and spongy tissue thickness, overall leaf thickness, chloroplast number, and cuticle thickness. Application of NSVCL and TH significantly elevated the net photosynthetic rate (Pn) by 86.55 % and 60.92 %, respectively, and increased total chlorophyll content (TChl) by 24.91 % and 11.76 %. Biostimulants facilitated nutrient uptake and boosted antioxidant enzyme activity in P. quinquefolius . Partial least squares path modeling (PLS-PM) further demonstrated that both NSVCL and TH promoted plant growth by enhancing soil enzyme activity in forest environments. These findings underscore NSVCL's efficacy in improving P. quinquefolius growth under forest conditions and provide a practical foundation for advancing organic forest cultivation and sustainable forest-medicine integration.
研究领域:
植物学
·
农业科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MICROBIOLOGY
·
2013
·Vol. 51
·期 2
·166-173
·
IF 1.276
Wu Qiong,Bai Linquan,Liu Weicheng,Li Yingying,Lu Caige,Li Yaqian,Fu Kehe,Yu Chuanjin,Chen Jie
Streptomyces lydicus A01 and Trichoderma harzianum P1 are potential biocontrol agents of fungal diseases in plants. S. lydicus A01 produces natamycin to bind the ergosterol of the fungal cell membrane and inhibits the growth of Botrytis cinerea. T. harzianum P1, on the other hand, features high chitinase activity and decomposes the chitin in the cell wall of B. cinerea . To obtain the synergistic biocontrol effects of chitinase and natamycin on Botrytis cinerea , this study transformed the chit42 gene from T. harzianum P1 to S. lydicus A01. The conjugal transformant (CT) of S. lydicus A01 with the chit42 gene was detected using polymerase chain reaction (PCR). Associated chitinase activity and natamycin production were examined using the 3, 5-dinitrosalicylic acid (DNS) method and ultraviolet spectrophotometry, respectively. The S. lydicus A01- chit42 CT showed substantially higher chitinase activity and natamycin production than its wild type strain (WT). Consequently, the biocontrol effects of S. lydicus A01- chit42 CT on B. cinerea , including inhibition to spore germination and mycelial growth, were highly improved compared with those of the WT. Our research indicates that the biocontrol effect of Streptomyces can be highly improved by transforming the exogenous resistance gene, i.e. chit42 from Trichoderma , which not only enhances the production of antibiotics, but also provides a supplementary function by degrading the cell walls of the pathogens.
研究领域:
微生物学
·
植物病理学
·
基因工程
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）