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Trichoderma viride
FOOD HYDROCOLLOIDS · 2019 · IF 5.839

Structural characteristics and functional properties of soluble dietary fiber from defatted rice bran obtained through Trichoderma viride fermentation

Mengyun Jia,Jiajun Chen,Xiaozhen Liu,Mingyong Xie,Shaoping Nie,Yi Chen,Jianhua Xie,Qiang Yu

Trichoderma viride was utilized to ferment the defatted rice bran (DRB), and soluble dietary fiber was prepared before and after fermentation, named as B-SDF and F-SDF, respectively. Optimum fermentation conditions for yield of F-SDF were determined using response surface methodology (RSM). The yield of F-SDF was significantly increased from 10.5 (w/w, %) to 33.4 (w/w, %) under the optimal conditions (inoculum size = 10%, pH = 5.8 and time = 41 h). Fourier transform infrared spectroscopy (FTIR) demonstrated that both B-SDF and F-SDF had typical characteristics of the infrared spectrum of polysaccharides , and X-ray diffraction (XRD) revealed they were mainly substances of amorphous structure. In addition, F-SDF showed looser structure by scanning electron microscopy (SEM) analysis, increased molecular weight by high-performance gel permeation chromatography (HPGPC) analysis, and more complex monosaccharide composition by high-performance anion exchange chromatography (HPAEC) analysis. Furthermore, functional properties analysis showed that F-SDF exhibited higher water-holding capacity (WHC), oil-holding capacity (OHC), water solubility (WS) and cholesterol absorption capacity (CAC) than B-SDF. In summary, the present study suggested that SDF from defatted rice bran obtained through Trichoderma viride fermentation has a higher potential to be used as a functional ingredient in foods .

研究领域: 食品科学 · 生物技术
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INTERNATIONAL BIODETERIORATION & BIODEGRADATION · 2017 · IF 2.962

Improved biodegradation potential of chlorobenzene by a mixed fungal-bacterial consortium

Zhuowei Cheng,Chao Li,Christian Kennes,Jiexu Ye,Dongzhi Chen,Shihan Zhang,Jianmeng Chen,Jianming Yu

A defined consortium of Ralstonia pickettii L2 (bacterium) and Trichoderma viride LW-1 (fungus) was selected to assess its potential for the enhanced biodegradation of mono-chlorobenzene (CB). At an initial concentration of 220 mg L −1 CB, the developed consortium showed an enhanced degradation rate of 0.50 mg CB·g −1 protein ·h −1 , while the individual Ralstonia sp. L2 and Trichoderma sp. LW-1 showed average degradation rates of 0.34 and 0.32 mg CB·g −1 protein ·h −1 , respectively. A CO 2 conversion level of up to 86.3% reflected a possible high mineralization extent of CB by the co-culture. The estimated μ max and v max values were 0.36 h −1 and 0.41 h −1 for the consortium, which were much higher than the values obtained by each strain individually. 2-Chlorophenol (2-CP) accumulated in the growth medium of strain L2 and inhibited its growth, but it could be consumed quickly by the fungus LW-1, providing a possibility to reach complete biodegradation of CB in a short time. Real-time PCR revealed that bacterium L2 played a major role in the initial stage, and that fungus LW-1 grew well if 2-CP was generated. These results suggest that the fungal-bacterial consortium might be effectively applied for complete biodegradation of CB and have a potential environmental implication in purification of CB-contaminated environments.

研究领域: 环境科学 · 微生物学
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Journal of Water Process Engineering · 2023 · IF 7

Diatomite-Trichoderma viride composite microspheres for selective removal of anionic dyes and copper ions

Shuang Xu,Yunqing Luo,Zhanlian Han,Tong Zhang,Lianzhi Sun,Gaonan Zheng,Keying Wang,Zhiqiang Cheng

Taking into account that Trichoderma viride dispersion mycelium existed many shortcomings, such as difficult to separate, easy to deactivate, and low adsorption performance, Diatomite -Trichoderma viride (DE- T. viride ) composite microspheres were designed and prepared by chemical bonding, and anion dye and copper ion synergistic removal strategies were proposed. The adsorption performance of the composite microspheres on CR and Cu 2+ were demonstrated by Zeta potential and Fourier infrared spectroscopy . The adsorption process followed the pseudo-second-order model, involving various mechanisms such as metal chelation, polysaccharide adhesion, hydrogen bonding, and electrostatic interactions. Intraparticle diffusion model identity that adsorption is controlled by multiple steps. Adsorption isotherms of CR and Cu 2+ displayed excellent agreement with the Langmuir, Freundlich, and Temkin models. Impressively, DE- T. viride spheres exhibited maximum adsorption capacities of 176.3 and 63.8 mg/g for CR and Cu 2+ . Moreover, they displayed selective adsorption for anionic dyes, with capacities 8 and 10 times higher than those for cationic and neutral ionic dyes, respectively. T. viride microspheres generates extracellular polymers, which are rich in a variety of functional groups and can adsorb metal ions through chelation and transport, moreover, it adsorbs anions with positive charge. It is worth mentioning that DE- T. viride spheres exhibited ease of separation, stability, and reproducibility even after multiple adsorption-recycling cycles, maintaining an impressive adsorption rate of 80 %. In conclusion, these multifunctional, efficient, and eco-friendly DE- T. viride spheres present promising potential for environmental remediation, particularly in removing contaminants from various environmental matrices. Their outstanding performance, selectivity , and recyclability make them a valuable and sustainable solution for addressing pollution issues and promoting environmental sustainability .

CAS / MDL: 573-58-0
研究领域: 环境科学 · 微生物学
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY · 2021 · IF 5.279

Bound Polyphenols from Insoluble Dietary Fiber of Defatted Rice Bran by Solid-State Fermentation with Trichoderma viride: Profile, Activity, and Release Mechanism

Jiayan Xie,Shuai Liu,Ruihong Dong,Jianhua Xie,Yi Chen,Guanyi Peng,Wang Liao,Puyou Xue,Lei Feng,Qiang Yu

This study is aimed at exploring the release of bound polyphenols (BP) from insoluble dietary fiber (IDF) and its mechanism by solid-state fermentation (SSF) via Trichoderma viride. The results indicated that BP released by SSF (5.55 mg GAE/g DW) was significantly higher than by alkaline hydrolysis. In addition, 39 polyphenols and catabolites were detected, and the related biotransformation pathways were speculated. Quantitative analysis showed that SSF released more ferulic acid, p-coumaric acid, and organic acids, which led to advances in antioxidant, α-amylase, and α-glucosidase inhibitory activities. Furthermore, structural characteristics (scanning electron microscopy, X-ray diffraction, thermos gravimetric analysis, and Fourier transform infrared spectroscopy) and dynamic changes of carbohydrate-hydrolyzing enzymes indicated that the destruction of hemicellulose and the secretion of xylanase were vital for releasing BP. Overall, this study demonstrated that SSF was beneficial to release BP from IDF, which could provide insight into utilizing agricultural byproducts in a more natural and economical way.

研究领域: 食品科学 · 生物技术 · 营养学 · 微生物学
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Food Bioscience · 2023 · IF 5.2

Mixed fermentation of navel orange peel by Trichoderma viride and Aspergillus niger: Effects on the structural and functional properties of soluble dietary fiber

Chenxi Yang,Jieqiong Yao,Ting Zhang,Kuimin Yang,jiaying Guo,Siyi Pan

Navel orange peel is a potential source of soluble dietary fiber. In order to make full use of this resource and improve its physiological activities, the effects of single and mixed strains fermentation modifications were compared in this study. The structural, physicochemical, and functional properties of soluble dietary fiber fermented by Trichoderma viride and/or Aspergillus niger were investigated. The results showed that mixed fermentation resulted in significantly higher activity of complex cellulase than single fermentation. On the second day, when T. viride and A. niger were inoculated at a ratio of 3:1, the activities of carboxymethyl cellulase , β -glucosidase, and filter paper lyase were 1.54, 1.61, and 1.30 times those of single fermentation. Mixed fermentation increased the yield of soluble dietary fiber from 6.63% to 15.73%. Scanning electron microscopy and Fourier transform infrared spectroscopic analyses suggested that soluble dietary fiber produced by mixed fermentation had smaller particle size, lower crystallinity, and more disrupted structure. Moreover, the functional properties of soluble dietary fiber were remarkably improved. After mixed fermentation, the glucose and cholesterol adsorption amounts, and α -amylase inhibition activity increased by 1.51, 1.63, and 1.76 times, respectively. In summary, mixed fermentation was an efficient and environmentally friendly modification method. Modified SDF can be utilized in the food processing industry, expanding the range of by-product applications.

研究领域: 食品科学 · 微生物学
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