JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Chen Wang,Yeyong Li,Lingjun Zeng,Chongli Shi,Yi Peng,Hui Li,Haibo Chen,Jun Yu,Jin Zhang,Biao Cheng,Ruolin Pan,Xiaoli Wang,Minghui Xiang,Yuan Huang,Yongdi Liu
Tris(1,3-dichloro-2-propyl) phosphate (TDCPP) has received concerns due to its frequent detection in environmental media and biological samples. Our previous study has indicated TDCPP reduced the lifespan of Caenorhabditis elegans ( C. elegans ) by triggering an unconventional insulin/insulin-like growth factor signaling (IIS) pathway. This study continued to investigate the possible deleterious effects of TDCPP relating to longevity regulation signal pathways and biological processes. Specifically, this study uniquely performed small RNA transcriptome sequencing (RNA-seq), focusing on the underlying mechanisms of TDCPP-reduced the longevity of C. elegans in-depth in microRNAs (miRNAs) . Based on Small RNA-seq results and transcript levels of mRNA involved in the unconventional IIS pathway, a small interaction network of miRNAs-mRNAs following TDCPP exposure in C. elegans was preliminarily established. Among them, up-regulated miR-48 and miR-84 ( let-7 family members) silence the mRNA of daf-16 (the crucial member of the FoxO family and pivotal regulator in longevity) via post-transcription and translation dampening abilities, further inhibit its downstream target metallothionein-1 ( mtl-1 ), and ultimately contributed to the reduction of nematode longevity and locomotion behaviors. Meanwhile, the high binding affinities of TDCPP with miRNAs cel-miR-48–5p and cel-miR-84–5p strongly support their participation in the regulation of nematode mobility and longevity. These findings provide a comprehensive analysis of TDCPP-reduced longevity from the perspective of miRNAs.
研究领域:
毒理学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Min Xia,Xuedong Wang,Jiaqi Xu,Qiuhui Qian,Ming Gao,Huili Wang
The organophosphate flame retardant, tris (1-chloro-2-propyl) phosphate (TCPP), is ubiquitous in environmental matrices; however, there is a paucity of information concerning its systemic toxicity. Herein, we investigated the effects of TCPP exposure on zebrafish neurodevelopment and swimming behavior to elucidate the underlying molecular mechanisms of neurotoxicity. Under TCPP gradient concentration exposure, the hatching rates were declined by up to 33.3% in 72 hpf, and the malformation rates increased from 15% to 50%. Meanwhile, TCPP led to abnormal behaviors including decreased locomotive activity in the dark and slow/insensitive responses to sound and light stimulation of larvae. TCPP caused excessive apoptosis and ROS accumulation in early embryonic development, with hair cell defects and structural deformity of neuromast. Abnormal expression of neurodevelopment ( pax6a , nova1 , sox11b , syn2a , foxo3a and robo2 ) and apoptosis-related genes ( baxa , bcl2a and casp8 ) revealed molecular mechanisms regarding abnormal behavioral and phenotypic symptoms. Chronic TCPP exposure led to anxiety-like behavior and excessive panic, lower capacity for discrimination and risk avoidance, and conditioned place preference in adults. Social interaction tests demonstrated that long-term TCPP stress resulted in unsociable, eccentric, lonely and silent behaviors in adults. Zebrafish memory and cognitive function were severely reduced as concluded from T-maze tests. Potential mechanisms triggering behavioral abnormality were attributed to histopathological injury of diencephalon, abnormal changes in nerve-related genes at transcription and expression levels, and inhibited activity of AChE by TCPP stress. These findings provide an important reference for risk assessment and early warning to TCPP exposure, and offer insights for prevention/mitigation of pollutant-induced nervous system diseases.
研究领域:
毒理学
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神经科学
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环境科学
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发育生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2021
·
IF 2.694
Renxin Wang,Yuanzuo Zou,Jingzhou Guo,Yuan Pu,Dan Wang
1,3,5-Tris(1-phenyl-1H-benzimidazol-2-yl)benzene (TPBi) is normally used as functional materials in organic light-emitting diodes (OLEDs) and the information about the solubilities of TPBi in organic solvents is crucial for the preparation of inks toward inkjet printing technology, which is considered to be a next-generation manufacturing method for OLED production. The solubilities of TPBi in 12 kinds of organic solvents, including n-hexane, cyclohexane, methanol, ethanol, isopropanol, n-butanol, acetonitrile, ethyl acetate, dimethyl sulfoxide, tetrahydrofuran, chlorobenzene, and trichloromethane, at the temperature range from 283.15 to 323.15 K and atmospheric pressure were determined based on the isothermal saturation method. The obtained solubility data were correlated by the modified Apelblat equation and the correlated results exhibited good agreement with the experimental data. The nanonization of TPBi was conducted via high gravity-assisted solvent/antisolvent precipitation processing, which showed a beneficial effect for film formation.
研究领域:
材料科学
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化学工程
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纳米技术
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有机电子学
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产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2017
·Vol. 52
·期 18
·11109-11119
·
IF 2.599
Zhu Hong,Li Rui,Wang Fanghui,Chen Nanjun,Li Ziming,Wang Zhiqian
To improve the conductivity of anion exchange membranes without reducing the mechanical properties, a series of poly ionic liquids (PILs) with precursors of 1,3,5-tris(1-imidazolyl)benzene (TIB) containing tri-conduct groups were synthesized and incorporated into quaternized poly (2,6-dimethyl phenylene oxide) (QPPO) membranes. The new poly ionic liquids we designed contain ternary conduct groups in one monomer which are advantageous to form ionic channels and thus dramatically increase the conductivity of membranes. Various alkyl chains were introduced into TIB during polymerization to tune the distance between two tri-conduct groups. The resulting PILs/QPPO composite membranes showed good thermal stability, appropriate mechanical properties, enhanced conductivity and fuel cell performance. When the numbers of –CH 2 – between two tri-conduct groups reached 12, the composite membrane showed the highest conductivity and fuel cell performance (55.5 mS/cm at 80 °C and 168.9 mW/cm 2 ). The composite membranes we prepared show potential application in anion exchange membrane fuel cells (AEMFCs).
研究领域:
材料科学
·
能源工程
✓ 已验证
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Jukun Xiong,Suyun Chen,Zixuan Wei-Xiong,Chang Yuan,Meicheng Wen,Wanjun Wang,Qinhao Lin,Yingxin Yu
In this study, carbon isotope analysis was employed to investigate hydrolysis and natural pyrite-activated persulfate (NP/PDS) degradation of TCPP. EPR and radical quenching experiments revealed that • OH and SO 4 • ⁻ were the dominant reactive oxygen species. The XPS, XRD, and FTIR results indicated Fe(Ⅱ) served as the primary active site, generating • OH and SO 4 • ⁻. For acidic and neutral hydrolysis, the apparent kinetic isotope effects ( 13 C-AKIE = 1.031 ± 0.004 and 1.030 ± 0.004, respectively) were consistent with the intrinsic KIE associated with C–O bond cleavage (1.00–1.03), suggesting that C–O bond cleavage was the initial rate-limiting step. In contrast, negligible carbon isotope fractionation during alkaline hydrolysis indicated that P–O bond cleavage was the initial rate-limiting step. For degradation in the NP/PDS, the observed 13 C-AKIEs were 1.009 ± 0.001, 1.004 ± 0.000, and 1.005 ± 0.001, respectively. These values were inconsistent with intrinsic KIEs expected for C-H (1.01–1.03) and C-Cl (1.02–1.03) bond cleavage but corresponded to the lower end of the intrinsic KIEs range for C–O bond cleavage, indicating that P–O bond cleavage was the initial rate-limiting step. Overall, this study demonstrates isotope analysis is a powerful tool for identifying the geochemical mechanisms of OPFRs in contaminated fields.
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Jinzhi Ren,Ying Peng,Luhang She,Lu Yan,Jianjun Li,Caixia Gao,Chao Wang,Yimeng Wang,Xiangping Nie,Xiaowei Zhang
The estuarine ecological environment faces significant threats from contaminants of emerging concern (CECs); yet, the risk posed by CECs to resident organisms remains poorly understood. Here, we employed tiered toxicity testing to investigate the adverse effects and potential mechanisms of tris (1-chloro-2-propyl) phosphate (TCPP) on the early life stages of an estuarine fish, Mugilogobius chulae . TCPP affected the development of M. chulae embryos, including survival, morphology, hatching, and behavior. A concentration-dependent transcriptomic analysis showed that TCPP disrupted 12 neurodevelopment-related KEGG pathways in M. chulae embryos, with five of the 30 % top-ranked pathways related to neurotransmitter signaling. Besides the cholinergic synapse signaling pathway, the glutamatergic signaling pathway (including NMDAR and AMPAR subtypes) may also mediate TCPP-induced neurodevelopmental toxicity. The NMDAR subtype GRIN2B was downregulated at high concentrations. Molecular dynamics simulations revealed a strong interaction between TCPP and GRIN2B, with TCPP binding to the residues Ile153 and Ile188. The results suggest that NMDARs play a crucial role in TCPP-induced neurodevelopmental toxicity toward M. chulae. AOP network analysis predicted that TCPP may impact cognitive functions and memory. Our study provides a novel testing strategy for identifying the mechanisms of toxicity of CECs, a crucial component of ecological risk assessment.
研究领域:
环境毒理学
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水生生态学
·
分子生物学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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