ChemistryOpen
·
2023
·Vol. 12
·期 3
·e202200259
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IF 2.3
Yulin Zhou,Jicheng Niu,Yan Zhou,Fei Li
Graphical An open liquid plasticine-based FASS system (FASS: field-amplified sample stacking) for protein enrichment was developed, with which proteins can be enriched 40–80-fold by LP-FASS within 40 min. Protein analysis is an important approach for disease diagnosis, in which sample pretreatment is an essential step since protein samples are often complex and many protein biomarkers are of low abundance. Here, given the good openness and light transmission of liquid plasticine (LP), which is a liquid entity formed by SiO 2 nanoparticles and encapsulated aqueous solution, we developed a LP-based field-amplified sample stacking (FASS) system for protein enrichment. The system was composed of a LP container, a sample solution and a Tris-HCl solution containing hydroxyethyl cellulose (HEC). The system design, mechanism investigation, optimization of experimental parameters and characterization of LP-FASS performance for protein enrichment were well studied. Under the optimized experimental conditions of 1 % HEC, 100 m m Tris-HCl and 100 V in the LP-FASS system, a 40–80 times enrichment of proteins was obtained in 40 min using bovine hemoglobin (BHb) as the model protein using the constructed LP-FASS system. The simultaneous enrichment of multiple proteins (phycocyanin, BHb and cytochrome C) was also realized using the system. The LP-FASS system can serve as a new platform for protein enrichment which is easy to be combined with online and offline detections.
研究领域:
分析化学
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蛋白质组学
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生物医学工程
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DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 63
·51392-51398
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IF 3.84
Jia-Ping Lai,Yue Zuo,Hui Sun,Ying Yu
The hydrophilic molecularly imprinted microspheres (HMIMs) functionalized with amino and carboxyl groups have been synthesized successfully using human hemoglobin as a template. The obtained HMIMs have been characterized using scanning electron microscopy (SEM), adsorption isotherms, dynamic binding as well as quartz crystal microbalance (QCM). The rebinding results indicate that the HMIMs exhibit highly selective recognition properties towards target human hemoglobin in Tris–HCl aqueous solution with an imprinting factor of 1.90 and a high binding capacity of 98 mg g−1. The QCM characterization results also demonstrate that the HMIMs coated electrode shows a more sensitive response to hemoglobin than that of a non-imprinted microspheres coated electrode.
研究领域:
材料科学
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分析化学
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生物化学
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DOI
高影响因子
ACS Applied Nano Materials
·
2021
·
IF 5.097
Fang-Nan Wu,Jian Zhu,Guo-Jun Weng,Jian-Jun Li,Jun-Wu Zhao
In this work, a tyrosine-decorated gold nanocluster (Tyr-GNC)–cerium(III) complex was established for the fluorescent detection of dopamine (DA). Tris–HCl buffer was used for synthesizing Tyr-GNC; thus, Tyr-GNC could emit fluorescence even under neutral conditions. The quantum yield (QY) of Tyr-GNC was 13.3%. Trivalent cerium ions (Ce3+) chelated hydroxyl and amino groups of tyrosine to form GNC–Ce3+. Also, DA showed a higher quenching efficiency in the fluorescence of GNC–Ce3+ than that of GNC. The mechanism was that Ce3+ chelated with DA and promoted its oxidative self-aggregation under alkaline buffer, and, at the same time, the oxidative product quenched the fluorescence of Tyr-GNC by the inner filter effect (IFE). In DA detection, GNC–Ce3+ showed a wide detection range (0.1–1000 μM) and a low detection limit (10.85 nM) for DA with high selectivity. The platform was successfully applied to DA detection in human plasma. This work proposed insight into the interaction between GNC–Ce3+ and DA, which has broad application prospects in clinical diagnosis.
研究领域:
纳米技术
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分析化学
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生物医学工程
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临床诊断
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DOI
高影响因子
INORGANICA CHIMICA ACTA
·
2023
·
IF 2.8
Zheng Li,Jing Wang,Xiaoxia Peng,Yu Chen,Mengyuan Geng
Hydrogen sulfide plays an essential roles in physiological and pathophysiological process. It is still a challenge to design a simple and reasonable H 2 S-specfic fluorescent probe. Herein, we develop a simple reaction-based fluorescent probe for detecting H 2 S in DMF/Tris-HCl buffer system. Fluorescence emission spectra were used to investigate the recognition performance of probe L towards hydrogen sulfide. The results shown that probe L has a specific recognition towards hydrogen sulfide over other tested metal cations and anions with a rapid response. The probe L combined with H 2 S in a 1: 1 manner with a detection limit of 0.372 μM. The appropriate pH range for detecting H 2 S was 4 ∼ 8. The proposed recognition mechanism was studied by fluorescence titration spectrum, Job’s plot, 1 H NMR titration experiment and mass spectrometry analysis. Moreover, probe L was applied in real water samples, test strips and fluorescent films to detect H 2 S successfully.
研究领域:
分析化学
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生物化学
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环境科学
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DOI
高影响因子
Small
·
2024
·
IF 13
Leqi Li,Xinyuan Ye,Zichong Ji,Meiqiong Zheng,Shihong Lin,Mingzhe Wang,Jiawei Yang,Pengcheng Zhou,Zongman Zhang,Binghao Wang,Haoyang Wang,Yan Wang
High-fidelity wireless electrophysiological monitoring is essential for ambulatory healthcare applications. Soft solid-like hydrogels have received significant attention as epidermal electrodes because of their tissue-like mechanical properties and high biocompatibility. However, it is challenging to develop a hydrogel electrode that provides robust contact and high adhesiveness with glabrous skin and hairy scalp for high-fidelity, continuous electrophysiological signal detection. Here, a paintable, fast gelation, highly adhesive, and conductive hydrogel is engineered for high-fidelity wireless electrophysiological monitoring. The hydrogel, consisting of gelatin, gallic acid, sodium citrate, lithium chloride, glycerol, and Tris-HCl buffer solution exhibits a reversible thermal phase transition capability, which endows it with the attributes of on-skin applicability and fast in situ gelation with 15 s, thereby addressing the aforementioned limitations. The introduction of gallic acid enhances the adhesive properties of the hydrogel, facilitating secure electrode attachment to the skin or hairy scalp. To accentuate the potential applications in at-home electrophysiological health monitoring, the hydrogel electrodes are demonstrated for high-fidelity electrocardiogram recording for one hour during various daily activities, as well as in simultaneous electroencephalogram and electrocardiogram recording during a 30 min nap.
研究领域:
生物医学工程
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材料科学
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电生理学
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医疗技术
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2018
·Vol. 6
·期 37
·6008-6015
·
IF 4.776
Fengyi Wang,Xuan Hu,Jing Hu,Qianqian Peng,Baozhan Zheng,Juan Du,Dan Xiao
In this study, bimetallic lanthanide coordination polymer nanoparticles (Tb-GMP-Eu CPNs) were developed for sensing alkaline phosphatase (ALP) by utilizing guanine monophosphate (GMP) as the bridge ligand to self-assemble with Tb3+ and Eu3+ in Tris–HCl buffer solution. In this polymer framework, an efficient energy transfer from Tb3+ to Eu3+ occurred because the emission energy of Tb3+ matched well with the absorption energy of Eu3+. As a result, the synthesized Tb-GMP-Eu CPNs exhibited the characteristic red emission of Eu3+ when excited at 290 nm. When ALP was introduced into the Tb-GMP-Eu CPNs system, it would destruct the combination between Tb and GMP by a dephosphorylation process, resulting in interruption of energy transfer from Tb3+ to Eu3+ and therefore the fluorescence quenching of Eu3+. Based on these facts, a fluorescence assay based on bimetallic lanthanide coordination polymer nanoparticles for ALP detection was developed. Such a Tb-GMP-Eu CPNs sensor exhibited sensitive response to ALP with a detection limit down to 0.004 U L−1. Moreover, the successful application of ALP inhibitor evaluation and determination of ALP in serum samples have been demonstrated. Therefore, the proposed method has great potential application for ALP detection in biological and clinical diagnosis.
研究领域:
分析化学
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生物化学
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纳米技术
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生物医学工程
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临床诊断
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2024
·
IF 1.6
Zheng Chang,Qiqi Zhao,Binghua Yao,Lei Han
Novel electroactive nanotags of Cu(II) functionalized polyethylenimine (PEI)/SiO2 nanoparticles (abbreviated as Cu2+/PEI/SiO2 NPs) were conveniently synthesized using a strong coordination reaction between Cu(II) and the multi-amino groups of PEI. These electroactive nanotags were easily coated on the overoxidized polypyrrole (OPPy) modified Nafion/multi-walled carbon nanotubes (abbreviated as Nafion/MWNTs) electrode surface through Cu-N bonds and strong electrostatic interactions. The Cu2+/PEI/SiO2 NPs@OPPy/MWNTs hybrid nanomaterial was formed without the addition of extra membrane-forming substances. The experimental results demonstrate that the OPPy/MWNTs composite membrane coupled with electrocatalytic Cu(Tris)42+ complexes in tris(hydroxymethyl)aminomethane (Tris)-HCl buffer medium amplified the electrochemical signals of nanotags based on the mutual synergistic effects. As an application, a label-free electrochemical immunosensor for epithelial ovarian cancer biomarker-carbohydrate antigen-125 (CA125) was constructed by covalently immobilizing CA125 antibody onto the hybrid nanomaterial modified electrode. With the increasing concentrations of CA125 antigen, different decreases in the electrochemical signals of the nanotags were observed due to the formation of antigen-antibody immune complexes. The electrochemical immunosensor showed a linear relationship from 0.001 to 500 U·mL−1 for CA125 with a detection limit of 3.4 × 10−4 U·mL−1. In addition, the proposed immunosensor was applied to the determination of CA125 in human serum with satisfactory results.
研究领域:
生物化学
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纳米技术
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分析化学
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癌症生物学
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电化学
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产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Ya-Qi Zhao,Zhi-Peng Li,Shi-Chao Dong,Hao Wang,Yi-Mei Zhao,Lin-Yi Dong,Zhen-Yu Zhao,Xian-Hua Wang
The effective method for trypsin purification should be established because trypsin has important economic value. In this work, a novel and simple strategy was proposed for fabricating micron-sized magnetic Fe 3 O 4 @agarose-benzamidine beads (MABB) with benzamidine as a ligand, which can efficiently and selectively capture trypsin. The micro-sized MABB, with clear spherical core-shell structure and average particle size of 6.6 μm, showed excellent suspension ability and magnetic responsiveness in aqueous solution. The adsorption capacity and selectivity of MABB towards target trypsin were significantly better than those of non-target lysozyme. According to the Langmuir equation, the maximum adsorption capacity of MABB for trypsin was 1946 mg g −1 at 25 °C, and the adsorption should be a physical sorption process. Furthermore, the initial adsorption rate and half equilibrium time of MABB toward trypsin were 787.4 mg g −1 min −1 and 0.71 min, respectively. To prove the practicability, MABB-based magnetic solid-phase extraction (MSPE) was proposed, and the related parameters were optimized in detail to improve the purification efficiency. With Tris-HCl buffer (50 mM, 10 mM CaCl 2 , pH 8.0) as extraction buffer, Tris-HCl buffer (50 mM, 100 mM CaCl 2 , pH 8.0) as rinsing buffer, acidic eluent (0.01 M HCl, 0.5 M NaCl, pH 2.0) as eluent buffer and alkaline buffer (1 M Tris-HCl buffer, pH 10.0) as neutralization solution, the MABB-based MSPE was successfully used for trypsin purification from the viscera of grass carp ( Ctenopharyngodon idella ). The molecular weight of purified trypsin was determined as approximate 23 kDa through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The purified trypsin was highly active from 30 °C to 60 °C, with an optimum temperature of 50 °C, and was tolerant to pH variation, exhibiting 85 % of maximum enzyme activity from pH 7.0 to 10.0. The results demonstrated that the proposed MABB-based MSPE could effectively purify trypsin and ensure the biological activity of purified trypsin. Therefore, we believe that the novel MABB could be applicable for efficient purification of trypsin from complex biological systems.
研究领域:
生物化学
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生物技术
·
分析化学
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蛋白质纯化
·
酶学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）