Introduction: Tuta absoluta is currently considered one of the most devastating invasive pests of solanaceous plants worldwide, causing severe damage to the tomato industry. Insects use volatile organic compounds (VOCs) to locate host plant for feeding and oviposition. Those VOCs could be developed as lures for pest monitoring and control.Methods: In this study, the differentially accumulated VOCs between the preferred host (tomato) and non-preferred host (eggplant) were analyzed by GC–MS method, and their roles on female T. absoluta host selection and egg laying behaviors were investigated using electroantennography (EAG), olfactometer and cage experiments.Results: A total of 39 differentially accumulated VOCs were identified in tomato and eggplant. Strong EAG signals were obtained in 9 VOCs, including 5 VOCs highly accumulated in tomato and 4 VOCs highly accumulated in eggplant. Further olfactometer bioassays showed that 4 compounds (1-nonanol, ethyl heptanoate, ethyl octanoate and o-nitrophenol) were attractive to T. absoluta females, while 5 compounds (2-phenylethanol, 2-pentylfuran, trans,trans-2,4-nonadienal, 2-ethyl-5-methylpyrazine and trans-2-nonenal) were repellent, indicating that VOCs from host plants play important roles in host plant preferences. The attractive activities of 1-nonanol and ethyl octanoate, as well as the repellent activities of trans,trans-2,4-nonadienal and trans-2-nonenal, were further confirmed in cage experiments.Discussion: In this study, two attractants and two repellents for T. absoluta were developed from plant released VOCs. Our results could be useful to enhance the development of eco-friendly and sustainable pest management strategies for T. absoluta.
Li Ma,Shengcun Zhang,Tianjie Zhang,Yingxin Zhang,Jinyu Zhao,Le Chang,Meiqin Wang,Xingtao Qie,Jie Li,Xizhong Yan
Tomato pinworm is a globally invasive pest of Solanaceae crops with rapidly evolving insecticide resistance. The “push–pull” method represents a potentially effective and environmentally friendly control tactic. Herein, four EAD-active volatile organic compounds (VOCs), cis-3-hexen-1-ol, β-phellandrene, nonanal, and 4-ethylbenzaldehyde, were identified from the volatiles of tomato, eggplant, and pepper. All the compounds elicited strong electrophysiological and attractive behavior in both female and male adults. Semifield trapping assays demonstrated that cis-3-hexen-1-ol, β-phellandrene, and nonanal significantly caught adults, with the most pronounced efficacy for β-phellandrene. The blend (same proportions as those in the headspace volatile) demonstrated an exceptionally strong trapping effect and exhibited superior performance compared with any compound tested individually under complex field conditions. These compounds were confirmed as key olfactory cues mediating host selection in Tuta absoluta, with the synthetic blend exhibiting particularly strong attractant properties, thus serving as an effective “pull” component in field-deployable “push–pull” strategy targeting this pest.
危险品化学品经营许可证（带存储）