FOOD CONTROL
·
2021
·
IF 5.548
Yaqi Hou,Jiao Liu,Yanchun Shao,Xitian Peng,Dawen Zhang,Lifang Hu,Fusheng Chen,Youxiang Zhou
Modified mycotoxins can cause an underestimation of exposure risk due to their untraceable characteristics. Hongqu , a traditional Chinese fermented product, is widely used as food colorants , fermenters and functional foods for about 2000 years. Though there were several methods to reduce citrinin (CIT) content in Hongqu , the risk assessment and prevention of modified CIT remain inadequate, causing potential risk to human health. The current study firstly identified the occurrence of modified CIT in Hongqu via acid, alkaline and enzyme hydrolysis combined with chromatographic and mass spectrometric approaches. Then 8 Hongqu samples and 5 Monascus products were treated using the optimized acid hydrolysis method and analyzed by ultra-high-performance liquid chromatography with fluorescence detector. The results showed that CIT content in the CIT positive Hongqu increased by 35.28%–458.13% after acid hydrolysis, demonstrating there could be a underestimation risk in the CIT positive Hongqu samples, and the modification of CIT might not be chemical modified, but matrix associated.
研究领域:
食品安全
·
微生物学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
GLYCOCONJUGATE JOURNAL
·
2016
·Vol. 33
·期 2
·219-226
·
IF 1.828
Yao Hong L.,Conway Louis P.,Wang Mao M.,Huang Kun,Liu Li,Voglmeir Josef
Herein we describe a UPLC-FLD-based method for the quantification of the sialic acid content of red meat, using a synthetic neuraminic acid derivative as an internal standard. X-Gal-α-2,6- N -propionylneuraminic acid was synthesized via a chemoenzymatic pathway and its hydrolytic stability was characterized. Known quantities of this compound were incubated with samples of red meat under sialic acid-releasing conditions. The released sialic acids were derivatized, analyzed by UPLC-FLD, and the Neu5Ac/Neu5Gc content of the meat sample was determined by comparison with the internal standard. A number of red meats were analyzed by this method with the following results (Neu5Ac μg/g tissue, Neu5Gc μg/g tissue ± s.d.): pork (68 ± 3, 15.2 ± 0.7), beef (69 ± 8, 36 ± 5), lamb (46 ± 2, 33 ± 1), rabbit (59 ± 2, 0.4 ± 0.4), and hare (50 ± 4, 1 ± 1). We envisage that this methodology will find application in investigating the health effects of dietary Neu5Gc.
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研究领域:
生物化学
·
食品科学
·
医学营养学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Ming Lv,Haidong Gao,Shucai Gao,Yeru Liu,Jingjing Yao,Hongwei Liu,Fayun Wang,Mantang Chen,Lianjun Song,Tianlin Wang,Libin Wan
Ochratoxin A (OTA) contamination in food crops has sparked global concern for potential health risk to humans and animals. A simple, accurate and sensitive method was developed for the determination of OTA using electromembrane extraction (EME) coupled with ultra-high performance liquid chromatography-fluorescence detector (UPLC-FLD). By optimizing the important factors, EME showed good performance for the extraction of OTA in spiked corn flour sample. Using the developed EME-UPLC-FLD method, good linearity for OTA was achieved in the range of 1–100 ng mL −1 , and the limit of detection (LOD) was 0.02306 μg kg −1 , which was well below the established maximum residue limit. The proposed method was successfully applied for the determination of trace OTA in multiple matrices (corn flour extract, corn flour slurry and soybean milk) with satisfactory recoveries ranging from 80.01 % to 116.7 % at four spiked levels, which has great potential to analyze trace chemical contaminant in food crops.
研究领域:
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Toxins
·
2023
·Vol. 15
·期 5
·337
·
IF 4.2
Baoxia Ni,Jin Ye,Zhihong Xuan,Li Li,Xiangrui Wen,Zongwang Li,Hongmei Liu,Songxue Wang
Sample pretreatment is a vital step in the detection of mycotoxins, and traditional pretreatment methods are time-consuming, labor-intensive and generate much organic waste liquid. In this work, an automatic, high-throughput and environmentally friendly pretreatment method is proposed. Immunomagnetic beads technology and dispersive liquid–liquid microextraction technology are combined, and the zearalenone in corn oils is directly purified and concentrated under the solubilization effects of surfactant. The proposed pretreatment method allows for the batch pretreatment of samples without pre-extraction using organic reagents, and almost no organic waste liquid is produced. Coupled with UPLC-FLD, an effective and accurate quantitative detection method for zearalenone is established. The recovery of spiked zearalenone in corn oils at different concentrations ranges from 85.7 to 89.0%, and the relative standard deviation is below 2.9%. The proposed pretreatment method overcomes the shortcomings of traditional pretreatment methods and has broad application prospects.Keywords:immunomagnetic beads;dispersive liquid liquid microextraction;pretreatment;zearalenone;corn oils;UPLC-FLDKey Contribution:An automatic and eco-friendly pretreatment method of zearalenone (ZEN) in corn oils was developed based on immunomagnetic beads with dispersive liquid–liquid microextraction technology. The proposed pretreatment method, coupled with UPLC-FLD, met the accuracy and sensitivity detection requirements of ZEN in corn oils.
研究领域:
食品科学
·
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）