FOOD CHEMISTRY
·
2013
·
IF 3.334
Guisheng Zhou,Hanqing Pang,Yuping Tang,Xin Yao,Yunhe Ding,Shaoqing Zhu,Sheng Guo,Dawei Qian,Juan Shen,Yefei Qian,Shulan Su,Li Zhang,Chun Jin,Yong Qin,Jin-Ao Duan
In this study, a rapid, simple and sensitive analytical method was developed for the quantitative determination of 20 nucleosides and nucleobases in functional foods at trace levels using hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HILIC-UPLC–TQ-MS/MS) in multiple-reaction monitoring (MRM) mode. Under optimised chromatographic conditions, good separation of 20 target compounds was achieved using a Waters Acquity UPLC BEH Amide column and gradient elution in 11 min. The limits of detection (LODs) and quantification (LOQs) were between 0.02–42.54 ng/mL and 0.05–98.18 ng/mL for the 20 analytes, respectively. This is the first report about simultaneous analysis of nucleosides and nucleobases in functional foods using this method, which afforded good linearity, precision, repeatability and accuracy. The method developed was successfully applied to quantify target compounds in batches of ginkgo seeds. The method potentially could be used to determine polar and trace-level nucleosides and nucleobases in ginkgo seeds.
研究领域:
分析化学
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食品科学
✓ 已验证
DOI
高影响因子
International Journal of Analytical Chemistry
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2015
·
IF 1
Yahui Ge,Yuping Tang,Sheng Guo,Xin Liu,Zhenhua Zhu,Pei Liu,Jin-ao Duan
Thais clavigera, as function food, is distributed widely along the coasts of China. To compare and tap its potentially nutritional and functional values, hydrophilic interaction ultra-performance liquid chromatography coupled with triplequadrupole tandem mass spectrometry (HILIC-UPLC-TQ-MS/MS) was used for simultaneous identification and quantification of amino acids, nucleosides, and nucleobases in the extracts of T. clavigera from 19 sea areas in China, and a PCA was further performed for comparing their content variation in different distribution regions. The total contents of amino acids varied from 116.74 mg/g to 298.58 mg/g being higher than contents of nucleosides and nucleobases that varied from 2.65 mg/g and 20.49 mg/g. Among the habitats, Hainan province had content advantages on others. By PCA, samples collected from different regions were classified into three groups. For specific constituents, lysine accounted for large part of essential amino acids; glycine and taurine also play important roles in the delicate taste and health care function of it. Inosine takes up most of total contents of nucleosides and nucleobases. These results provided good data for establishing quality standard of T. clavigera related products and their further development and utilization.
研究领域:
海洋生物学
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营养学
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分析化学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
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2016
·Vol. 21
·期 4
·408
·
IF 2.465
Yahui Ge,Yuping Tang,Sheng Guo,Xin Liu,Zhenhua Zhu,Lili Zhang,Pei Liu,Shaoxiong Ding,Xiangzhi Lin,Rurong Lin,Jin-ao Duan
To evaluate the nutritional and functional value ofSipunculus nudus, a rapid, simple and sensitive analytical method was developed using ultra-high performance liquid chromatography coupled with a triple quadrupole mass detection in multiple-reaction monitoring mode for the simultaneous quantitative determination of 25 free amino acids and 16 nucleosides and nucleobases inS. nuduswithin 20 min, which was confirmed to be reproducible and accurate. The limits of detection (LODs) and quantification (LOQs) were between 0.003–0.229 μg/mL and 0.008–0.763 μg/mL for the 41 analytes, respectively. The established method was applied to analyze 19 batches ofS. nudussamples from four habitats with two different processing methods. The results showed thatS. nuduscontained a variety of free amino acids, nucleosides and nucleobases in sufficient quantity and reasonable proportion. They also demonstrated that the contents of these compounds in different parts ofS. nuduswere significantly discriminating, which were in the order: (highest) coelomic fluid > body wall > intestine (lowest). The method is simple and accurate, and could serve as a technical support for establishing quality control ofS. nudusand other functional seafoods. Moreover, the research results also laid foundation for further exploitation and development ofS. nudus.Keywords:UPLC-TQ-MS/MS;Sipunculus nudus;free amino acid;nucleoside;nucleobase
研究领域:
海洋生物学
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食品科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2023
·
IF 8.8
Xiaohan Wang,Bing Li,Shanshan Sun,Qingqing Liu,Jin Zhu,Xian Zhou,Huijin Zhang,Qian Wu,Liangsheng Wang
Antique Lotus ( Nelumbo ) is a perennial aquatic plant with unique historical significance and cultural value, whereas its potential economic value hasn’t been fully explored. The present study showed that lotus seedpods had significantly higher antioxidant capacity than other parts by FRAP, ABTS, and ORAC assays and analyzed the proanthocyanidins and flavonols in the seedpods of Antique Lotus. Polyphenols contributed to great antioxidant activity and 51 polyphenols were identified by UPLC-TQ-MS analysis. In which, 27 compounds were identified from lotus seedpods for the first time, including 20 trimers, 5 dimers and 2 tetramers of proanthocyanidin. Total proanthocyanidins explained 70%–90% of the different antioxidant activities and the content of proanthocyanidin trimers showed the strongest correlations with the antioxidant activities. This study provided a fundamental reference for the research of polyphenols in lotus and found that Antique Lotus seedpod extracts have the promising prospects of additives used in feed and food processing.
研究领域:
植物学
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植物化学
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食品科学
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农业化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 7
·2114
·
IF 4.927
Yi Li,Xia-Jin Liu,Shu-Lan Su,Hui Yan,Sheng Guo,Da-Wei Qian,Jin-Ao Duan
Present studies have shown that Flos Chrysanthemi has anti-inflammatory and other effects and regulates intestinal function, while the chrysanthemum stem and leaf as non-medicinal parts of chrysanthemum have similar chemical components with chrysanthemum, but the activity and mechanisms are rarely elucidated. Therefore, this study used a DSS-induced zebrafish inflammatory bowel disease model to study the anti-inflammatory and antioxidant effects of chrysanthemum stem and leaf extracts. The results indicate that DSS induction leads to increased secretion of acidic mucin in the intestines of juvenile fish, enlargement of the intestinal lumen and the emergence of intestinal inflammation. Compared with the model group, each administration group differentially inhibited the expression of IL-1β, IL-8 and MMP9 in DSS-induced zebrafish, while upregulating the activity of superoxide dismutase. The quantitative analysis results showed that the flavonoids (including Linarin, Diosmetin-7-glucoside, Tilianin, etc.) and phenolic acids (including Isochlorogenic acid C, Isochlorogenic acid A, 1,3-Dicaffeoylquinic acid, etc.) in the alcohol extract were closely related with both anti-inflammatory and antioxidant activity, while the polysaccharides were also shown a certain anti-inflammatory and antioxidant activity. In conclusion, this study suggests that the flavonoids, phenolic acids and polysaccharides from chrysanthemum stem and leaf extracts can improve inflammatory bowel disease of zebrafish by regulating the expressions of IL-1β, IL-8 and MMP9.Keywords:Flos Chrysanthemi;chrysanthemum stem and leaf;inflammatory bowel disease (IBD);zebrafish;UPLC-TQ/MS
研究领域:
药理学
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炎症研究
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水生毒理学
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分析化学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2024
·
IF 2.9
Zhang Zhiqi,Li Lin,Wang Xinyang,Liu Liyan
Houttuynia cordata Thunb. (H.cordata) , as a traditional Chinese medicinal herb and food, has been used to treat various diseases because of rich content in flavonoids. Here, an ultra-high performance liquid chromatography with triple quadrupole tandem mass spectrometry (UPLC-TQ-MS/MS) method was proposed for the determination of flavonoids profiling in H.cordata . Single factor experiment and response surface methodology were used to optimize the pretreatment process of H.cordata samples. The optimal conditions were as follows: ultrasonic temperature: room temperature (25 °C); ultrasonic time: 5 min; solid-liquid ratio: 1:40 and concentration of ethanol: 60%. The method showed the good linearity ( r ≥ 0.9855), the satisfactory intra-day (RSD%≤10.94%) and inter-day precision (RSD% ≤ 11.2%) and low limit of quantification (< 0.01 µg/mL). The content of flavonoids in flowers and leaves was much higher than in roots and stems. The flavonoids in the leaves of H.cordata from Chongqing was the highest (9.22 mg/g), and that in the flowers of H.cordata from Hubei province was the highest (6.69 mg/g). In addition, the antioxidant activity of the extracts of H.cordata was evaluated using the scavenging effect of the extracts on five free radicals and the iron ion reducing ability. The best antioxidant activity was observed in scavenging DPPH free radicals with EC 50 value of 295.16 µg/mL. The scavenging of ABTS•+ free radicals and PTIO• free radicals was also effective with EC 50 values of 818.82 µg/mL and 685.43 685.43 µg/mL, respectively. Thus, this study will be helpful to the development and utilization of flavonoids from H.cordata in food and pharmaceutical industries.
研究领域:
生药学
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分析化学
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食品科学
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药学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Mengyang Hou,Yujuan Zhou,Ka Wing Cheng,Rongrong Luo,Chengyuan Lin,Lin Zhu,Zhaoxiang Bian
This study first established a green, integrated process coupling ultrasound-assisted natural deep eutectic solvent (UA-NADES) extraction with macroporous resin purification to obtain Chinese quince total phenolics (CQ-TP). L-proline/glycerol (molar ratio 2:5; Pro-Gly) was identified as the optimal extraction solvent among eleven synthesised NADESs and four common solvents. The optimised UA-NADES extraction process (35% water in Pro-Gly, 40 °C, 300 W, 36 min, liquid-to-solid ratio 30 mL/g) yielded 59.14 ± 0.65 mg/g CQ-TP, markedly higher than the yields from conventional methods. NKA-9 resin showed superior adsorption and desorption performance for CQ-TP, with adsorption behavior consistent with the Langmuir isotherm and a pseudo-second-order kinetic model. Column purification achieved a CQ-TP purity of 72.54 ± 0.81%. Finally, nine main phenolic constituents in the purified CQ-TP extract were quantified by UPLC–TQ–MS/MS to enable batch-to-batch consistency monitoring. This work will significantly contribute to the valorization of Chinese quince phenolics and the development of natural antioxidants.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）