FOOD CHEMISTRY
·
2013
·
IF 3.334
Guisheng Zhou,Hanqing Pang,Yuping Tang,Xin Yao,Yunhe Ding,Shaoqing Zhu,Sheng Guo,Dawei Qian,Juan Shen,Yefei Qian,Shulan Su,Li Zhang,Chun Jin,Yong Qin,Jin-Ao Duan
In this study, a rapid, simple and sensitive analytical method was developed for the quantitative determination of 20 nucleosides and nucleobases in functional foods at trace levels using hydrophilic interaction ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HILIC-UPLC–TQ-MS/MS) in multiple-reaction monitoring (MRM) mode. Under optimised chromatographic conditions, good separation of 20 target compounds was achieved using a Waters Acquity UPLC BEH Amide column and gradient elution in 11 min. The limits of detection (LODs) and quantification (LOQs) were between 0.02–42.54 ng/mL and 0.05–98.18 ng/mL for the 20 analytes, respectively. This is the first report about simultaneous analysis of nucleosides and nucleobases in functional foods using this method, which afforded good linearity, precision, repeatability and accuracy. The method developed was successfully applied to quantify target compounds in batches of ginkgo seeds. The method potentially could be used to determine polar and trace-level nucleosides and nucleobases in ginkgo seeds.
研究领域:
分析化学
·
食品科学
✓ 已验证
DOI
高影响因子
International Journal of Analytical Chemistry
·
2015
·
IF 1
Yahui Ge,Yuping Tang,Sheng Guo,Xin Liu,Zhenhua Zhu,Pei Liu,Jin-ao Duan
Thais clavigera, as function food, is distributed widely along the coasts of China. To compare and tap its potentially nutritional and functional values, hydrophilic interaction ultra-performance liquid chromatography coupled with triplequadrupole tandem mass spectrometry (HILIC-UPLC-TQ-MS/MS) was used for simultaneous identification and quantification of amino acids, nucleosides, and nucleobases in the extracts of T. clavigera from 19 sea areas in China, and a PCA was further performed for comparing their content variation in different distribution regions. The total contents of amino acids varied from 116.74 mg/g to 298.58 mg/g being higher than contents of nucleosides and nucleobases that varied from 2.65 mg/g and 20.49 mg/g. Among the habitats, Hainan province had content advantages on others. By PCA, samples collected from different regions were classified into three groups. For specific constituents, lysine accounted for large part of essential amino acids; glycine and taurine also play important roles in the delicate taste and health care function of it. Inosine takes up most of total contents of nucleosides and nucleobases. These results provided good data for establishing quality standard of T. clavigera related products and their further development and utilization.
研究领域:
海洋生物学
·
营养学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2016
·Vol. 21
·期 4
·408
·
IF 2.465
Yahui Ge,Yuping Tang,Sheng Guo,Xin Liu,Zhenhua Zhu,Lili Zhang,Pei Liu,Shaoxiong Ding,Xiangzhi Lin,Rurong Lin,Jin-ao Duan
To evaluate the nutritional and functional value ofSipunculus nudus, a rapid, simple and sensitive analytical method was developed using ultra-high performance liquid chromatography coupled with a triple quadrupole mass detection in multiple-reaction monitoring mode for the simultaneous quantitative determination of 25 free amino acids and 16 nucleosides and nucleobases inS. nuduswithin 20 min, which was confirmed to be reproducible and accurate. The limits of detection (LODs) and quantification (LOQs) were between 0.003–0.229 μg/mL and 0.008–0.763 μg/mL for the 41 analytes, respectively. The established method was applied to analyze 19 batches ofS. nudussamples from four habitats with two different processing methods. The results showed thatS. nuduscontained a variety of free amino acids, nucleosides and nucleobases in sufficient quantity and reasonable proportion. They also demonstrated that the contents of these compounds in different parts ofS. nuduswere significantly discriminating, which were in the order: (highest) coelomic fluid > body wall > intestine (lowest). The method is simple and accurate, and could serve as a technical support for establishing quality control ofS. nudusand other functional seafoods. Moreover, the research results also laid foundation for further exploitation and development ofS. nudus.Keywords:UPLC-TQ-MS/MS;Sipunculus nudus;free amino acid;nucleoside;nucleobase
研究领域:
海洋生物学
·
食品科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Mengyang Hou,Yujuan Zhou,Ka Wing Cheng,Rongrong Luo,Chengyuan Lin,Lin Zhu,Zhaoxiang Bian
This study first established a green, integrated process coupling ultrasound-assisted natural deep eutectic solvent (UA-NADES) extraction with macroporous resin purification to obtain Chinese quince total phenolics (CQ-TP). L-proline/glycerol (molar ratio 2:5; Pro-Gly) was identified as the optimal extraction solvent among eleven synthesised NADESs and four common solvents. The optimised UA-NADES extraction process (35% water in Pro-Gly, 40 °C, 300 W, 36 min, liquid-to-solid ratio 30 mL/g) yielded 59.14 ± 0.65 mg/g CQ-TP, markedly higher than the yields from conventional methods. NKA-9 resin showed superior adsorption and desorption performance for CQ-TP, with adsorption behavior consistent with the Langmuir isotherm and a pseudo-second-order kinetic model. Column purification achieved a CQ-TP purity of 72.54 ± 0.81%. Finally, nine main phenolic constituents in the purified CQ-TP extract were quantified by UPLC–TQ–MS/MS to enable batch-to-batch consistency monitoring. This work will significantly contribute to the valorization of Chinese quince phenolics and the development of natural antioxidants.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）