Journal of Cosmetic Dermatology
·
2019
·Vol. 18
·期 6
·1899-1906
·
IF 1.311
Jie Tang,Lidan Xiong,Xiaohong Shu,Wei Chen,Wenjia Li,Jing Li,Lianzheng Ma,Ying Xiao,Li Li
Summary Background Excessive free radicals, generated from the metabolic reaction in organisms, have been implicated in many human diseases as well as aging process. Nowadays, many synthetic substances have been developed as anti-oxidation cosmetic ingredients. However, man-made antioxidants often have certain toxicity and side effects, which make their application under strict control. Therefore, more and more researchers focus on natural antioxidants because of their advantages. Aims In this study, CE obtained from natural Chinese medicine was used to investigate whether it had antioxidant effect in vitro and repair effect on HaCaT cell damage caused by UVB. Methods UV-Vis and HPLC were adopted for qualitative and quantitative analysis of CE. We investigated the antioxidant potential of CE by assessing its ABTS + , DPPH•, hydroxyl (OH•), and superoxide anions ( ) free-radical quenching ability. The safety of CE was studied by CCK-8 assay. To evaluate the anti-oxidation effect of CE on UVB-induced damage on HaCaT cells, superoxide dismutase (SOD) activity, and malondialdehyde (MDA) content were tested. Results Experiment data showed that the CE displayed high scavenging ability: ABTS + , DPPH•, OH•, and quenching rates were 88%, 64%, 94%, and 58%, respectively. Furthermore, after UVB radiation (30 mJ/cm 2 ), adding CE (50-500 μg/mL) could increase the SOD activity in HaCaT cells and reduce the MDA contents. Conclusions All results illustrate that the CE shows significant antioxidant effect on scavenging free radicals in vitro. Besides, the CE can repair UVB-induced oxidant damage by improving SOD activity and reducing MDA content.
研究领域:
药理学
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皮肤生物学
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天然产物化学
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PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Kang wei,Lu He,Xuan Li,Meng Wu,Haijie Wang,Liangcong Hu,Huanhuan Xu,Yixuan Zhang,Ling Zhou,Xiaojuan Xu,Liang Guo
Excessive exposure to ultraviolet (UV) radiation results in premature aging of the skin. Lentinan polysaccharide (LNT), has been reported in the literature to exhibit a variety of biological functions, such as anti-inflammatory, antioxidant, and anticancer activities. In this research, the function and underlying mechanisms of LNT in UVB-induced photoaging were evaluated in vivo and in vitro . Initially, we developed a UVB-induced photoaging model in HDFs. The results indicated that following UVB irradiation, the positive rate of β-galactosidase, the proportion of dead cells, the generation of ROS, the expression of the senescence-associated secretory phenotype (SASP), the mRNA levels of inflammatory factors, as well as the activity of matrix metalloproteinases (MMPs) were upregulated in HDFs. In contrast, the components of the extracellular matrix (ECM), were decreased. Simultaneously, the impact of UVB exposure on the dorsal skin of mice exhibited a similar pattern. LNT was demonstrated to effectively mitigate the aforementioned changes and inhibit UVB-induced skin photoaging and damage in mice. Mechanistically, our findings revealed that LNT inhibited UVB-activated nuclear factor kappa B (NF-κB), the mitogen-activated protein kinase (MAPK) pathways and the P21/P16 axis. This property of LNT presents considerable potential for the development of therapeutic strategies aimed at delaying skin photoaging.
研究领域:
皮肤生物学
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分子生物学
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药理学
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光老化研究
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天然产物药理
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细胞衰老机制
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信号转导
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PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2020
·
IF 3.573
Ying Wu,Yong Qian,Aicheng Zhang,Hongming Lou,Dongjie Yang,Xueqing Qiu
To solve the poor absorbance on ultraviolet A (UVA) radiations and dark color, alkali lignin (AL) was modified via atom transfer radical polymerization (ATRP) grafting of 2-4-benzoyl-3-hydroxyphenyl acrylate (BHA). The color of AL was whitened 86% and the UVA/UVB absorbance ratio of AL-g-BHA improved to 1.2, while the reported highest value was 0.9. The excellent UV-blocking performance of AL-g-BHA was demonstrated in sunscreen. Sun protection factor (SPF) value of the sunscreen containing 10 wt % of optimal AL-g-BHA reached 59. Nanoscale AL-g-BHA normal and reverse micelles (LBNM and LBRM) were fabricated to investigate the effect of microstructure on the sunscreen performance. When BHA chains were packed inside, the SPF value of sunscreen containing 10 wt % of LBNM decreased to 28. In contrast, the SPF value of sunscreen containing 10 wt % of LBRM with BHA chains distributing on the surface was up to 244. Both AL-g-BHA and corresponding micelles exhibited good biocompatibility.
研究领域:
材料科学
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生物学
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化学工程
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高影响因子
SKIN RESEARCH AND TECHNOLOGY
·
2023
·Vol. 29
·期 3
·e13303
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IF 2.2
Chunxia Gan,Titi Liu,Xiaorong Jia,Xueqin Huang,Xiangdong Qin,Xuanjun Wang,Jun Sheng,Huanhuan Xu
Background Skin photoaging is the damage caused by excessive exposure to ultraviolet (UV) irradiation. We investigated the effect of adenosine triphosphate (ATP) supplementation on UVB-induced photoaging in HaCaT cells and its potential molecular mechanism. Materials and methods The toxicity of ATP on HaCaT cells was examined by the MTT assay. The effects of ATP supplementation on the viability and apoptosis of HaCaT cells were determined by crystal-violet staining and flow cytometry, respectively. Cellular and mitochondrial ROS were stained using fluorescent dyes. Expression of Bax, B-cell lymphoma (Bcl)-2, sirtuin (SIRT)3, and superoxide dismutase (SOD)2 was measured via western blotting. Results ATP (1, 2 mM) exerted no toxic effect on the normal growth of HaCaT cells. UVB irradiation caused the apoptosis of HaCaT cells, and ATP supplementation inhibited the apoptosis induced by UVB significantly, as verified by expression of Bax and Bcl-2. UVB exposure resulted in accumulation of cellular and mitochondrial reactive oxygen species (ROS), but ATP supplementation suppressed these increases. Expression of SIRT3 and SOD2 was decreased upon exposure to UVB irradiation but, under ATP supplementation, expression of SIRT3 and SOD2 was reversed, which was consistent with the reduction in ROS level observed in ATP-treated HaCaT cells after exposure to UVB irradiation. Conclusions ATP supplementation can suppress UVB irradiation-induced photoaging in HaCaT cells via upregulation of expression of SIRT3 and SOD2.
研究领域:
细胞生物学
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分子生物学
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皮肤科
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DOI
PubMed
高影响因子
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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2021
·
IF 2.351
Chijian Zhang,Yongjie Lu,Yong Ai,Xian Xu,Siyang Zhu,Bing Zhang,Minghui Tang,Lanyue Zhang,Tinggang He
Glabridin, a compound of the flavonoid, has shown outstanding skin-whitening and anti-aging properties, but its water insolubility limits its wide application. Therefore, glabridin liposome (GL) has been developed to improve its poor bioavailability, while there are few studies to evaluate its amelioration of UVB- induced photoaging. This study is performed to investigate the amelioration of GL against UVB- induced cutaneous photoaging. The prepared GL has a spheroidal morphology with an average diameter of 200 nm. The GL shows lower cytotoxicity than glabridin, but it has a more effective role in inhibition of melanin. Moreover, the application of GL can effectively relieve UV radiation induced erythema and leathery skin, associated with the down-regulated expression of inflammatory cytokines (TNF-α, IL-6 and IL-10). Taken together, these results demonstrate that GL has potentials as topical therapeutic agents against UVB radiation induced skin damage through inhibiting inflammation.
研究领域:
药学
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皮肤病学
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化妆品科学
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PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2018
·
IF 2.297
Xia Lv,Zhongxiao Cong,Zhihao Liu,Xiaodong Ma,Ming Xu,Yan Tian,Xinyi Zhang,Bingqing Xu,Jianbin Zhang,Zeyao Tang
Topical application of trans -resveratrol was limited due to its insolubility and instability problems. In this work, three kinds of essential oil based microemulsions containing rose oil (RO-ME), jasmine oil (JO-ME) and lemon oil (LO-ME) were prepared, constituted of Cremophor EL/1,2-propanediol/essential oils (47: 23: 30, w/w). All the three microemulsions could significantly improve the solubility and photostability of trans -resveratrol. Moreover, they could sustain the release of trans -resveratrol. Antioxidant activity study demonstrated that trans -resveratrol loaded microemulsions exhibited higher scavenging activities than both trans -resveratrol and blank microemulsions. The cell experiments demonstrated that RO-ME and JO-ME had lower cytotoxicity on HaCaT cells than LO-ME. The trans -resveratrol loaded RO-ME and JO-ME could significantly protect the cells from UVB damage. Finally, the in vitro skin permeation study exhibited that the microemulsions could enhance the skin permeation of trans -resveratrol in comparison to the aqueous solution. In summary, the essential oil based microemulsions could improve the solubility, photostability, antioxidant activity and UVB photoprotection of trans -resveratrol, which will be beneficial to its topical application.
研究领域:
药剂学
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化妆品学
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药理学
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化学
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高影响因子
Biomolecules
·
2026
·Vol. 16
·期 3
·387
·
IF 5.6
Fangfang Chen,Yihan Cai,Jinxiong Wu,Nengzhen Fang,Fei Li,Hongtan Wu,Yu-Pei Chen
Taxifolin, a natural flavonoid, consistently exerts cytoprotective effects against various oxidative stresses. In this study, we systematically evaluated its photoprotective efficacy and underlying mechanisms against ultraviolet B (UVB)-induced injury in human immortalized keratinocytes (HaCaT). Cell viability and apoptosis were assessed by MTT, fluorescence staining, and flow cytometry, while integrative transcriptomic and proteomic analyses were employed to identify core pathways and key mediators. Taxifolin exhibited antioxidant capacity comparable to that of ascorbic acid under identical in vitro radical-scavenging assays. Moreover, it displayed a strong absorption peak at 289 nm that overlaps the UVB spectrum (280–320 nm), enabling it to act as a chemical sunscreen. In UVB-challenged HaCaT cells, taxifolin markedly reduced intracellular reactive oxygen species (ROS) and attenuated JNK/p38 MAPK activation, as evidenced by Western blot, thereby breaking the ROS-MAPK vicious cycle. Multi-omics revealed that taxifolin was associated with attenuation of UVB-imposed G1/S arrest concomitant with restored Cyclin expression, while up-regulating MYC, FOXQ1, HMOX1 and AP-1 components c-Jun/c-Fos and thereby switching on a pro-survival transcriptional program. Consequently, apoptosis was suppressed and survival was significantly improved. Collectively, taxifolin integrated chemical filtration, ROS scavenging and signaling modulation to support a multi-target photoprotective network, which provides mechanistic insight into taxifolin-mediated cytoprotection and identifies candidate molecular nodes for further validation.
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高影响因子
Journal of Cosmetic Dermatology
·
2022
·Vol. 22
·期 3
·1046-1062
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IF 2.189
Shasha Wang,Yuxin Shi,Jiapeng Ma,Zhuofei Ye,Miaomiao Yao,Jing Shang,Jianping Liu
Dragon's Blood is a member of the Chinese medicinal herb, having anti-oxygen and anti-inflammatory activity for the photoprotective effect. However, the poor water solubility of raw Dragon's Blood powder has limited its intradermal delivery process. In this study, we evaluated nanosuspensions to enhance intradermal delivery of Dragon's Blood exerting a photoprotective effect. The prepared nanosuspension was added to a composite hydrogel patch matrix for better skin application. In the present research, we used biocompatible materials hyaluronic acid and amino acid surfactants as nanosuspension stabilizers and agar/gelatin/sodium polyacrylate as hydrogel patch matrix. The prepared Dragon's Blood nanosuspension had a particle size of 447.0 ± 48.6 nm. The micro-structures morphology and viscoelasticity characteristics by SEM and rheological testing confirmed a sufficient crosslinked hydrogel network. The skin retention amount of Dragon's Blood nanosuspension was 1.48 times of raw Dragon's Blood powder water suspension, and the skin penetration amount of Dragon's Blood nanosuspension was only about 1/3 of Dragon's Blood DMSO solution. In the UVB-irradiated HaCaT cell phototoxicity model, Dragon's Blood nanosuspension also significantly increased cell viability by about 1 time of the model group and decreased the production of reactive oxygen species about 1/2 times of model group. In vivo safety and efficiency evaluation experiment illustrated that DB-NS hydrogel patch processes have favorable safety and photoprotective effect with no skin irritancy and phototoxicity. Furthermore, DB-NS and DB-NS hydrogel patches could protect skin from UVA and UVB irritating skin reactions. Overall, our study of the combined use of biocompatible and biodegradable materials as excipients of nanosuspension and hydrogel patch could be used as an effective additive of Intradermal delivery and skin photoprotection.
研究领域:
药剂学
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纳米技术
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皮肤病学
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药学
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DOI
PubMed
高影响因子
Drug Design Development and Therapy
·
2026
·
IF 6.1
Liwei Feng,Linjie Tian,Jing Guo,Lingling Zhang,Peng Wang,Xiaolin Bu
Introduction Ultraviolet B (UVB) irradiation is a key exogenous factor contributing to skin photoaging. Idebenone (IDE), a structural analog of coenzyme Q10 (CoQ10), exhibits potent free radical scavenging capacity and is mainly employed in the treatment of neurodegenerative disorders, and in anti-aging products. However, the molecular mechanisms underlying its photoprotective effects in skin cells remain poorly understood.Purpose This study aims to investigate the protective effects of IDE against UVB-induced photoaging in human immortalized epidermal keratinocytes (HaCaT cells).Methods We applied IDE to a UVB-induced HaCaT cell photoaging model and found that it significantly ameliorated the photoaging damage caused by UVB irradiation.Results Specifically, cell morphology recovered from a shrinkage state toward normal, the positivity rate of senescence-associated β-galactosidase (SA-β-gal) decreased, and enhanced the impaired cell migration and proliferation capacities. Additionally, oxidative stress imbalance was alleviated, as evidenced by a significant reduction in reactive oxygen species (ROS) and malondialdehyde (MDA) levels, along with an increase in superoxide dismutase (SOD) activity. Impaired mitochondrial function was restored, reflected by the repolarization of mitochondrial membrane potential (MMP) and an increase in adenosine triphosphate (ATP) synthesis. Furthermore, the elevated secretion levels of pro-inflammatory factors such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-8 (IL-8) were significantly reduced. Transcriptomic analysis and protein validation revealed that IDE significantly downregulated the expression of IL-3RA and effectively restored the expression of TUBA8.Conclusion IDE significantly improved UVB-induced photoaging damage in HaCaT cells through multiple pathways, including restoring cell viability, alleviating oxidative stress damage, reducing pro-inflammatory factor infiltration, and protecting mitochondrial function. Its mechanism of action may involve the regulation of targets such as IL-3RA and TUBA8.
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高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2024
·Vol. 59
·期 3
·1724-1737
·
IF 2.6
Yihan Sun,Jiaying Ren,Xiang Li,Kailing Sun,Dingding Feng,Xiuping Dong,Hang Qi
Summary Rich in numerous nutrients, undaria pinnatifida ( UP ) exhibited a broad spectrum of biological properties, including anti-inflammatory, anti-bacterial, anti-cancer, and antioxidant properties. A sexual pigment protein complex known as phycocyanin (PC) was the perfect photosensitiser and had no negative effects. Due to their superior qualities, hydrogels were also widely employed in tissue engineering, medication administration, and food packaging at the same time. The aim of this work was to investigate the impact of UVB irradiation on the gel characteristics of PC- UP hydrogels using UVB irradiation technology rather than chemical crosslinking approach. The results showed that the G ′ value of PC- UP hydrogel increased from 114.17 ± 0.58 Pa to 156.72 ± 0.82 Pa and the G ″ value increased from 36.80 ± 0.79 Pa to 58.05 ± 1.06 Pa after UVB irradiation. At the same time, the texture properties of hydrogel were significantly improved, and the hardness, adhesion and chewiness were increased to 214.02 ± 3.85, 198.51 ± 4.25 and 195.81 ± 5.69, respectively. After UVB irradiation, hydrogen bond was formed in PC- UP hydrogel to improve its thermal stability. In conclusion, the results show that PC decomposes with alginic acid, potassium salt, and sodium salt in UP under UVB irradiation to form a gel network structure, which enhances its gel properties. This study provides guidance for the photo response of PC- UP hydrogels, provides ideas for the application of PC- UP hydrogels, and broadens the application field of future hydrogels.
研究领域:
食品科学
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生物材料学
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海洋生物学
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高影响因子
Foods
·
2023
·Vol. 12
·期 24
·4392
·
IF 5.2
Rui Wang,Chaolong Rao,Qiuyan Liu,Xiong Liu
Zanthoxylumoleoresin, a concentrated extract derived fromZanthoxylum bungeanum, is rich in non-volatile, intensely flavorful substances and amide compounds, such as hydroxy-α-sanshool, hydroxy-β-sanshool, and hydroxy-ε-sanshool. The production process ofZanthoxylumoleoresin remains unstandardized, and there is still a lack of research on the precise classification and quantification of its key chemical constituents, as well as the stability of these compounds when produced using different extraction methods. This study utilized preparative liquid chromatography to extract and purify amide compounds fromZanthoxylumoleoresin, successfully isolating three sanshools: hydroxy-α-sanshool, hydroxy-β-sanshool, and hydroxy-ε-sanshool. The stability of three these sanshools under UVB irradiation in different solvents was explored in UVB-simulated sunlight conditions to investigate the degradation or transformation mechanism ofZanthoxylumalkylamides under UVB irradiation. The findings indicate a rapid decrease in the hydroxy-α-sanshool content under UVB ultraviolet light, aligning with the second-order kinetics. This study revealed alterations in the contents of hydroxy-α-sanshool, hydroxy-β-sanshool, and hydroxy-ε-sanshool and the formation of a new compound following exposure to UVB light. This new compound, along with the three sanshools, possesses a uniformm/z264 and shares similar chemical structures. Further analysis also uncovered that these compounds are capable of undergoing isomerization reactions under UVB irradiation. This demonstrates that UVB irradiation of certain intensities can modify the concentrations and chemical structures of theseZanthoxylumalkylamides. These insights offer crucial guidance for future studies on the processing and preservation ofZanthoxylumalkylamides and their derivatives.Keywords:Zanthoxylumalkylamides;UVB light;degradation;transformation;mechanism
研究领域:
食品科学
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植物化学
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PubMed
高影响因子
DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 42
·15539-15548
·
IF 4
Shanshan Zhao,Benchun Li,Tiantian Shen,Fang Fang,Songlin Zhuang,Dawei Zhang,Dechao Yu
Development of novel ultraviolet (UV) upconversion materials has been emerging as a hot research topic for application in tunable UV lasers, photocatalysis, sterilization, tagging, and most recently luminescence thermometry. We readily synthesized a series of Ho3+/Gd3+ co-doped LiYO2 upconversion phosphors by a traditional high-temperature reaction. Under excitation from a blue ∼445 nm laser, LiYO2:Ho3+,Gd3+ polycrystalline powders yield intense sharp ultraviolet B (UVB) upconversion luminescence from Gd3+ 6Pj (j = 7/2, 5/2, 3/2) excited states. By means of steady and dynamic photoluminescence spectra, we systematically investigated the involved two-photon absorption upconversion as well as the accompanying energy transfer processes between Ho3+ and Gd3+ ions in the LiYO2 host lattice. Interestingly, the distinguishable UVB luminescence constituents from Gd3+ 6Pj excited states exhibit sensitive temperature dependence in a 353–673 K range. Shedding light on thermal equilibria between Gd3+ 6Pj UV-emitting levels, their luminescence intensity ratios follow Boltzmann statistics for the application of new luminescence thermometry. For the scheme of 6P7/2–6P3/2 thermally coupled levels, it works over a temperature range of 373–673 K with a maximum relative sensitivity (Sr) of about 1.07% K−1 at 373 K, and its 6P7/2–6P5/2 counterpart works over 353–533 K with a maximum Sr of about 0.83% K−1 at 353 K. Overall, our study provides a new pathway to develop UV upconversion materials, and promotes the application of Gd3+-related UV luminescence constituents in sensitive temperature sensing over a wide temperature range.
研究领域:
材料科学
·
光学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）