SCIENCE OF THE TOTAL ENVIRONMENT
·
2017
·
IF 4.9
Cui Zhang,Jian Lu,Jun Wu,Yongming Luo
Ulva prolifera ( U. prolifera ) has been frequently involved in terrible algal proliferation in coastal areas. Although it is known to be associated with green tide, its contribution to the natural attenuation of the polycyclic aromatic hydrocarbons (PAHs) in seawater has not been evaluated. In this study, the removal of phenanthrene using U. prolifera collected from coastal water with green tide blooming was investigated. The results showed that phenanthrene could be removed efficiently in the presence of both the live and heat-killed U. prolifera . The phenanthrene concentrations of the live algae treatment decreased smoothly from 10.00 to 0.80 μg L − 1 through the whole process, while those of the heat-killed algae treatment decreased sharply from 10.0 to 2.71 μg L − 1 in one day and kept constantly after that. The in situ monitoring and visualizing using laser confocal scanning microscopy (LCSM) confirmed the accumulation of phenanthrene in U. prolifera . The increase in nutrient and temperature led to the increase of phenanthrene removal rate, while the salinity had less influence on the removal of phenanthrene. The removal efficiency by U. prolifera had a good linear relationship with phenanthrene initial concentration (r 2 = 0.999) even at 100 μg L − 1 which was higher than its environmentally relevant concentrations. High removal efficiency (91.3%) was observed when the initial phenanthrene concentration was set at environmental relevant concentration (5 μg L − 1 ). Results of this study demonstrate a potential new natural attenuation process for typical PAHs in coastal water during the outbreak of green tide. These findings indicate that the outbreak of harmful green tide algae may bring positive environmental benefits in the terms of the removal of harmful organic pollutants from coastal waters.
研究领域:
环境科学
·
海洋生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2021
·Vol. 11
·期 1
·1-12
·
IF 4.38
Yang Chenghu,Miao Shichao,Li Tiejun
The influences of water washing treatment on the properties of Ulva prolifera -derived biochar ( U.P- biochar) and its sorption characteristics of ofloxacin (OFL) were investigated. The results showed that the water washing treatment significantly changed the physiochemical structures of U.P- biochars, and improved the sorption capacity of OFL. The sorption capacity of OFL by U.P -biochar was closely dependent on pyrolysis temperature (200–600 °C) and equilibrium solution pH (3–11). Different sorption mechanisms (e.g. cation exchange, electrostatic attraction, H-bond and cationic–π and π–π interactions) were dominant for specific U.P- biochars under various pH regions (acidic, neutral and alkaline). Moreover, the unwashed and washed U.P- biochars prepared at 200 °C (BC200 and BCW200) showed a higher sorption capacity of OFL at pH = 7. The two-compartment first-order model provided an appropriate description of the sorption kinetics of OFL by BC200 and BCW200 ( R 2 > 0.98), which revealed that the contribution ratios between the fast and slow sorption compartments ( f fast /f slow , 1.55 for BC200 and 1.25 for BCW200) reduced after water washing treatment of U.P -biochar. The values of n for the Freundlich model were less than 1, which demonstrated that the sorption of OFL by BC200 and BCW200 was favourable and nonlinear. Also, the sorption of OFL by BC200 and BCW200 increased with an increase in solution temperature and the sorption process was spontaneous and endothermic. This study provides valuable information for being a primary consideration in the production and application of U.P- biochar.
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
WATER RESEARCH
·
2025
·
IF 12.4
Xinyu Li,Cui Zeng,Xiurong Han,Liqiang Fan,Jing Zhao,Yingzhe Wang,Xiulin Wang,Keqiang Li
Multitudinous dissolved organic nitrogen (DON) enters seawater from Ulva prolifera green tides impacting phytoplankton community structure in the Yellow Sea. Field investigations and cultural experiments on U. prolifera -derived DON aging revealed its regulatory effects. The green-tide outbreak area of the Southern Yellow Sea exhibits a seasonal cycle where dominance shifts from diatoms to dinoflagellates effected by young to aging U. prolifera -derived DON from summer to spring. Diatom abundance rose significantly following the summer green tide outbreak, fueled by young, protein-rich DON released by U. prolifera . After autumn and winter aging, U. prolifera -derived DON was dominant from protein-like to humic-like components, and dinoflagellates bloomed in the green tide outbreak area. An aging U. prolifera -derived DON adding culture experiment of Chaetoceros curvisetus and Prorocentrum minimum proved that old and young DON promoted dinoflagellate and diatom growth, respectively. Diatoms preferred 0 and 12 days-aged DON (DON 0 and DON 12 ) with tyrosine B and tryptophan T components, whereas dinoflagellate favored 80 days-aged DON (DON 80 ) with humic acid E and UV fulvic acid A components. DON 0 and DON 12 can be absorbed by diatoms with higher uptake ( k up ) and growth ( k G ) constants than by dinoflagellates under low leucine aminopeptidase (LAP) conditions. Dinoflagellates absorb old DON with higher k up and k G values under higher LAP concentrations than diatoms. This was consistent with the loop of U. prolifera -derived DON turning over the seasonal succession of diatom dinoflagellates in the green-tide outbreak area. This study revealed mechanism of seasonal dinoflagellate- U. prolifera -diatom cycle, and provided new insights for impacts of U. prolifera green tides on phytoplankton regime shift, thus, acting as a basis for marine management strategies.
研究领域:
海洋生物学
·
生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Science China-Earth Sciences
·
2016
·Vol. 60
·期 2
·391-396
·
IF 1.655
Li Jing,Sun Lei,Yu ZhiMing,Song XiuXian
Prevention of annual “green tides” caused by blooms of the green macroalga Ulva prolifera , which have occurred in the Yellow Sea since 2007, has received much attention. Increasing evidence indicates that micro-propagules on the rafts used for Porphyra yezoensis aquaculture along the coastline of Jiangsu Province in China, were the primary source of these green tides. In this study, we investigated the effectiveness of a silicone antifouling coating for preventing the adhesion and germination of U. prolifera micro-propagules on bamboo and nylon ropes, which have been used in rafts for P. yezoensis aquaculture. Our results demonstrated that a coating of silicone (Sylgard 184) containing 1.0 wt.% sodium benzoate (NaB) could reduce the adhesion rate to <5% and completely inhibited the growth of U. prolifera propagules on the coated surfaces. Coating bamboo and nylon ropes with NaB-incorporated silicone thus proved a potentially effective method for controlling the initial biomass accumulation of U. prolifera .
研究领域:
海洋生物学
·
环境科学
·
水产养殖
✓ 已验证
产品关联
DOI
高影响因子
AQUATIC TOXICOLOGY
·
2020
·
IF 4.346
Naicheng Li,Mengmeng Tong,Patricia M. Glibert
Ulva prolifera is a macroalgae that forms massive blooms, negatively impacting natural communities, aquaculture operations and recreation. The effects of the natural products, eugenol, β-myrcene, citral and nonanoic acid on the growth rate, antioxidative defense system and photosynthesis of Ulva prolifera were investigated as a possible control strategy for this harmful taxon. Negative effects on growth were observed with all four chemicals, due to the excessive production of reactive oxygen species and oxidative damage to the thalli. However, the response of U. prolifera under the four chemicals stress was different at the cellular level. β-myrcene, the most effective compound in terms of growth inhibition, induced oxidative stress as shown by the damage of total antioxidant capacity (T-AOC) and the downregulation of the glutathione-ascorbate (GSH-ASA) cycle which inhibited the antioxidative system. This chemical also inhibited photosynthesis and photoprotection mechanisms in U. prolifera , resulting in growth limitation. In contrast, U. prolifera was less affected by the second tested chemical, eugenol, and showed no significant change on photosynthetic efficiency in the presence of the chemical. The inhibition effects of the third and fourth tested chemicals, nonanoic acid and citralon, on growth and on the antioxidant defense system in U. prolifera were inferior. These results provide a potential avenue for controlling green tides in the future.
研究领域:
海洋生物学
·
生态毒理学
·
植物学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
·
2025
·
IF 1.6
Chen Yanan,Sui Wenxuan,Peng Jianbiao,He Xindi,Zhu Kairuo
In this work, through the ball-milling treatment process, the novel micron-engineered Ulva prolifera (m- Ulva ) was prepared and used to remove U(VI) from water. The m- Ulva properties were interpreted by analysis of the morphology structure, specific surface area, functional groups, and composition. The adsorption experimental results testified that the U(VI) adsorption process depends strongly on pH, while followed the pseudo-second-order kinetic model and the Langmuir adsorption isotherm model. The maximum absorption capacity was shown to be 147.06 mg/g at pH 5.0 and 25° C. Based on various characterization, the related adsorption mechanisms mainly encompassed electrostatic attraction, complexation and partial reduction of U(VI). The study presents a green, scalable method to transform Ulva prolifera into low-cost, micron-engineered biomass material for U(VI)-contaminated wastewater cleanup.
研究领域:
环境科学
·
海洋生物学
·
生物修复
·
核化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
AQUACULTURE
·
2024
·
IF 3.9
Jianan Jiang,Nianjun Xu,Ruihong Zhang,Wandi Qie,Xuan Zhou,Yahe Li
Summary The major reactive oxygen species superoxide (O 2 − ) plays important roles in plant growth, development, and stress responses. However, the molecular mechanism underlying O 2 − mediation of reproduction in Ulva prolifera is unclear. Here, we used inhibitors and promoters alongside phenotypes, histochemical staining, transcriptomic analysis, complementation tests, and related physiology parameter assays to determine that O 2 − was the main ROS that regulated reproduction cell formation by modulating the ascorbate-glutathione cycle. We found that O 2 − was the main ROS involved in regulating reproduction and was regulated by Ca 2+ levels. Analysis of the effects of different sources of O 2 − showed that chloroplast O 2 − mainly affected early reproductive progress and mitochondrial O 2 − impacted the entire reproduction process by regulating energy metabolism. Moreover, ascorbic acid (AsA) transmitted oxidative signals through the AsA-GSH cycle, thus interacting with O 2 − . However, an imbalance between AsA and ROS significantly impacted the oxidative signal transduction required for reproduction. Additionally, transcriptomic analysis revealed that the expression of genes related to O 2 − production and signal transmission, such as DHAR, were significantly downregulated by DPI and EGTA treatment, while the upregulated expression of GR, GSS, and GST could increase GSH content, resulting in a high GSH/GSSG ratio, which weakened oxidative signaling and disrupted reproduction. Taken together, our findings demonstrated that Ca 2+ level might regulate O 2 − production, and through DHAR affect the redox state change of the AsA-GSH cycle involved in signal transmission, which participates in the ROS signal transmit pathway and affects reproduction. These results provide new viewpoint about the reproduction and breeding of U. prolifera , then provide a scientific basis for land-based cultivation.
研究领域:
植物生物学
·
分子生物学
·
细胞生物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Pharmacology
·
2026
·
IF 5.4
Xiaochun Xia,Qian Zhou,Hui Su,Li Liu,Jiaxuan Chen,Yiting Chen,Shaoqing Lin,Xulan Zhou,Juan Wang
Background Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder, with prenatal exposure to valproic acid (VPA) being a recognized environmental risk factor for ASD, closely associated with its neurotoxic mechanism and oxidative stress. Marine green algae, such as Ulva prolifera , are rich sources of bioactive polysaccharides known for their antioxidant, anti-inflammatory, lipid-lowering, anti-tumor, and neuroprotective properties, with a core focus on antioxidant effects. Therefore, this study aims to investigate the protective effects and potential mechanisms of Ulva prolifera polysaccharides (PUPs) on oxidative stress damage induced by VPA in mouse hippocampal neuronal HT22 cells. Method The primary structure of PUPs was determined through infrared spectroscopy, liquid chromatography, and gel permeation chromatography. PUPs intervention in the VPA-induced HT22 oxidative damage cell model was used to measure oxidative stress factor levels. Results PUPs were characterized as composite polysaccharides with α-glycosidic bonds, sulfate ions, and aldonic acids, containing mannose, rhamnose, glucuronic acid, glucose, galactose, and xylose. The main molecular weight (Mw) was 2.124 kDa (80.535%). In vitro experiments revealed that PUPs significantly increased cell viability in the VPA model, enhanced intracellular SOD and CAT activities to boost antioxidant capacity, and concurrently reduced ROS levels and MDA content. Western blot analysis revealed that PUPs increased Nrf2, HO-1, and IκBα levels, while decreasing the expression levels of Keap1, p-NF-κB-p65/NF-κB-65, p-Erk/Erk, p-p38/p38, and p-JNK/JNK. Conclusions PUPs demonstrate a significant protective effect against VPA-induced oxidative stress damage in HT22 neuronal cells. As a natural antioxidant, PUPs hold promising potential for the prevention and adjunct treatment of ASD-related neuronal oxidative stress damage.
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2026
·
IF 12.5
Yuhan Lin,Xueyan Yan,Mengru Zhang,Chuanyi Wang
A marine biomass-based evaporator was made from Ulva prolifera and sodium alginate for green, low-cost solar desalination. • COMSOL simulation showed that the inverted cone structure promoted Marangoni convection for directional salt crystallization. • The evaporation and salt recovery rates reached values of 3.02 kg·m −2 ·h −1 and 3.13 kg·m −2 ·day −1 in 10 wt% NaCl solution. Solar-driven interfacial evaporation technology for saline water desalination, with advantages such as environmental friendliness and low cost, is of great significance for alleviating global water scarcity. However, salt scaling on the evaporator surface can reduce the evaporation rate and seriously threaten the system stability. Here, a sustainable inverted cone-shaped solar evaporator was fabricated using Ulva prolifera and sodium alginate as raw materials, achieving the synergy of efficient freshwater production and salt recovery. Under 1 sun irradiation, the fully marine biomass-based evaporator exhibited evaporation rates of 3.37 kg·m −2 ·h −1 and 3.02 kg·m −2 ·h −1 for 3.5 wt% and 10 wt% NaCl solutions, respectively, with no significant decay after 5 cycles. The unique 3D inverted-cone structure combined with the concentration gradient-induced Marangoni convection (verified by COMSOL simulation) promoted the saline radial transport and directional crystallization, enabling a salt collection rate of 3.13 kg·m −2 ·day −1 in 10 wt% NaCl. Furthermore, laboratory and outdoor performance tests using simulated seawater and real seawater demonstrated its application potential. This study provides a novel strategy for efficient seawater desalination and resource utilization. Download: Download high-res image (255KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
AQUACULTURE
·
2022
·
IF 5.135
Meihua Fan,Xiaowen Tang,Zongxin Yang,Jianxin Wang,Xiaolin Zhang,Xiaojun Yan,Peng Li,Nianjun Xu,Zhi Liao
Heat stress is one of the most remarkable abiotic factors limiting the growth and productivity of Ulva prolifera . The calcium signalling pathway plays an important role in the plant heat resistance response. However, the physiology and molecular mechanisms by which the calcium signalling pathway regulates the U. prolifera response to high temperature remain largely unknown. Changes in the physiology, transcriptome , and proteome of U. prolifera in response to high temperature were investigated with exogenous application of CaCl 2 (denoted UpCa) and the calmodulin (CaM) inhibitor W-7 (N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride, denoted UpW7) to fill this gap. In total, 33,475 differentially expressed genes (DEGs) and 869 differentially expressed proteins (DEPs) were identified between the control and UpCa treatments. At the mRNA level, the upregulated DEGs were enriched in the RNA transport , mRNA surveillance, carotenoid biosynthesis, basal transcription factor, porphyrin and chlorophyll metabolism, diterpenoid biosynthesis, and flavonoid biosynthesis pathways. The downregulated DEGs were enriched in photosynthesis, plant hormone signal transduction , photosynthesis-antenna proteins, and oxidative phosphorylation . At the protein level, upregulated proteins were enriched in alpha-linolenic acid metabolism, photosynthesis-antenna proteins, and plant–pathogen interactions. The downregulated proteins were enriched in endocytosis , mismatch repair, ribosomes, RNA degradation, and spliceosomes . The addition of exogenous CaCl 2 induced the expression of peroxidase , heat shock 70, heat shock 90, nuclear pore complex protein Nup62, MFS transporter, calcium-dependent protein kinase, calcium/calmodulin-dependent protein kinase kinase 1, calcium-binding protein calmodulin-like (CML), Ca 2+ -transporting ATPase, and serine/threonine-protein kinase at both the mRNA and protein levels. In addition, the same downregulated DEPs and DEGs were correlated and included the ApaG protein, chaperonin GroEL , proteasome inhibitor subunit 1 (PI31), serine/arginine repetitive matrix protein 1, glutamine synthetase, and F-type H + -transporting ATPase subunit alpha. The addition of W7 inhibited Ca 2+ -related gene expression, and the expression of genes and proteins involved in phagosome , photosynthesis-antenna proteins, RNA transport, plant–pathogen interactions, protein processing in the endoplasmic reticulum , and protein export were downregulated. However, exogenous CaCl 2 and W7 increased the expression of TFs, such as Myb and G2-like. In a subsequent study, the addition of CaCl 2 significantly increased and W7 significantly inhibited catalase and superoxide dismutase activity in U. prolifera under high-temperature stress. The expression of the CaM gene was significantly increased at 24 h in U. prolifera under high-temperature stress; CaCl 2 and W7 significantly inhibited the expression of the CaM gene. In summary, Ca 2+ activated Ca 2+ -related channels to regulate downstream transcript and response proteins; however, the addition of Ca 2+ and W7 inhibited photosynthesis. These results provide insights into the mechanism by which calcium increases the mechanical strength of U. prolifera in response to high-temperature stress.
研究领域:
植物学
·
分子生物学
·
环境科学
✓ 已验证
DOI
高影响因子
Foods
·
2025
·Vol. 14
·期 13
·2202
·
IF 5.1
Jiasi Liu,Zhiyong Li,Huiyue Gu,Songdong Shen
Large-scale blooms ofUlva proliferaseverely impact coastal ecosystems and economic development. In addressing Ulva management, the development of high-value utilization approaches for this macroalga remains crucial. Compared to other marine algae,Ulva proliferaexhibits higher protein content with diverse amino acid profiles, and existing studies demonstrate that hydrolyzedUlva proliferaproteins can yield biologically active peptides with functional potential. Conventional methods for producing bioactive peptides are often cost-intensive. Here, we employed in silico enzymatic hydrolysis to generate small peptides fromUlva proliferaprotein. Through computer screening, molecular docking with the Keap1 protein, and molecular dynamics simulations, we identified a potential antioxidant peptide, DWS (Asp-Trp-Ser). Molecular docking and dynamics simulations revealed that DWS forms stable complexes with Keap1 by establishing hydrogen bonds and Pi bonds with conserved amino acid residues (Leu557, Gly558, Ile559, Val604, Val606, and Arg415). In vitro antioxidant assays demonstrated that DWS exhibits potent DPPH and ABTS radical scavenging activities as well as reducing power. Cellular experiments showed that DWS effectively alleviates LPS-induced oxidative stress and inflammation in RAW264.7 macrophages.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）