STAR Protocols
·
2023
·
IF 0
Jingtan Zhu,Xiaomei Liu,Jianyi Xu,Zhang Liu,Yating Deng,Junyao Dai,Tingting Yu,Dan Zhu
Summary The majority of fluorescent vessel labeling techniques currently available are limited by their expense, incomplete labeling, or complexity. Here, we present VALID ( v essel l a beling via ge l atin-based l i pophilic d ye solution)—a protocol for complete labeling of different vascular networks. We describe steps for preparing different dye hydrogels, murine vascular casting and tissue harvesting, immunolabeling, tissue clearing, and imaging, as well as detailed analysis of the vascular networks. This protocol is helpful for evaluating vascular lesions in studying different vessel-associated diseases. For complete details on the use and execution of this protocol, please refer to Zhu et al. 1
研究领域:
生物学
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医学成像
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血管生物学
✓ 已验证
DOI
PubMed
Journal of Materials Chemistry C
·
2018
·Vol. 6
·期 41
·11178-11183
·
IF 5.976
Yan Gao,Yao Cheng,Tao Hu,Zeliang Ji,Hang Lin,Ju Xu,Yuansheng Wang
In this study, a double-perovskite Pr3+:Gd2ZnTiO6 thermometric phosphor is designed and successfully synthesized for the first time via a high-temperature solid-state method. By taking advantage of the intervalence charge transfer state (IVCT) interfered Pr3+ luminescence, the synthesized phosphor exhibits excellent optical thermometric performance in terms of both the fluorescence intensity ratio and luminescence lifetime. Specifically, by using the fluorescence intensity ratio between Pr3+: 3P0 → 3H4 and 1D2 → 3H4 transitions as a temperature detecting signal in the range of 293–433 K, the maximum absolute and relative sensitivities reach as high as 0.63 K−1 and 1.67% K−1, respectively; taking the fluorescence lifetime of the 1D2 state as a detecting signal in the range of 433–593 K, the corresponding sensitivities are 0.096 μs K−1 and 1.48% K−1. The results demonstrate that a thermal reading with high sensitivity over a wide range of temperature can be realized by this novel dual-mode design.
研究领域:
材料科学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2019
·Vol. 38
·期 3
·213-219
·
IF 2.804
Qian Chao,Fan Zhen-Guo,Zheng Wei-Wen,Bei Run-Xin,Zhu Tian-Wen,Liu Si-Wei,Chi Zhen-Guo,Aldred Matthew P.,Chen Xu-Dong,Zhang Yi,Xu Jia-Rui
High-performance low- k and low-loss circuit materials are urgently needed in the field of microelectronics due to the upcoming Fifth-Generation Mobile Communications Technology (5G Technology). Herein, a facile design strategy for non-fluorinated intrinsic low- k and low-loss polyimides is reported by fully considering the secondary relaxation behaviors of the polymer chains. A new amorphous non-fluorinated polymer (TmBPPA) with a k value of 2.23 and a loss tangent lower than 3.94 × 10 −3 at 10 4 Hz has been designed and synthesized, which to the best of our knowledge is the lowest value amongst the non-fluorinated and non-porous polymers reported in literature. Meanwhile, TmBPPA exhibits excellent overall properties, such as excellent thermostability, good mechanical properties, low moisture absorption, and high bonding strength. As high-performance flexible circuit materials, all these characteristics are highly expected to meet the present and future demands for high density, high speed, and high frequency electronic circuit used in 5G wireless networks.
研究领域:
材料科学
·
电子工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Lei Ding,Dandan Li,Sihang Zhang,Shuo Zhang,Zhaoyang Wang,Fanghui Du,Pengfang Zhang,Feng Yang
Lithium metal batteries (LMBs) attract widespread attention under current high energy density developments. However, wild Li dendrite propagations owing to chaotic Li depositions raise challenges for LMB blossoming. Separators with specific demands thus should be involved when smoothly transferring into LMBs. In this research, a fascinating P(TPC@Lys-Li) separator is prepared by the turbulent interfacial polymerization and electrospinning to ingeniously clarify dependencies of Li + transfer dynamics on double electric layer thickness (DELT) regulations within porous skeletons. P(TPC@Lys-Li) enables negatively charged porous skeleton surface and compresses DELT, which constructs high-efficiency Li + fast-transfer channels on porous skeletons and accelerates Li + transfer speed by 18.3 times. Especially, P(TPC@Lys-Li) supplies extra Li + for stable formations of solid electrolyte interphase (SEI) layer, homogenizing nucleation and growth behaviors during Li depositions. Remarkable C-rate capacity and cycle stability thus arise for assembled LMBs, holding 87.2 % capacity retention after 700 cycles at 0.5C even under high cathode loading and lean electrolyte conditions. P(TPC@Lys-Li) also maintains constant physical scale and unabated mechanical strength even at elevated temperatures approaching 200 °C, which provides excellent battery security as LMBs encounter uncontrollable thermal runaway. Above attractive features enable P(TPC@Lys-Li) separator to be potentially applied in LMBs demanding sufficient security, high-capacity density, and fast charge technology.
研究领域:
材料科学
·
电化学
·
能源存储与转换
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）