COLLOIDS AND SURFACES B-BIOINTERFACES
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2022
·
IF 5.999
Dan Zhao,Huan Liu,Zhixia Zhang,Xincai Xiao,Jun Li
The studies on the preparation of high-quality and long-wavelength-emitting silicon quantum dots and their application in biochemical detections have attracted increasing interests from researchers. In particular, the exploration of the effect of SiQDs and their complex in antibacterial and inhibition of biofilm areas, is of great significance to human health. This paper reports the fast synthesis of green-fluorescent SiQDs (TC-SiQDs, QYs=25%, Em=520 nm) with the synergistic effect of thiourea and catechol through microwave-assisted one-step hydrothermal route. Based on internal filtering effect, TC-SiQDs were then successfully employed as selective probes to detect vitamin B 12 , with detection range at 0.05–30 μM and limit of detection at 50 nM. Thanks to the excellent biocompatibility and fluorescence property of TC-SiQDs, they have been applied in bacterial imaging. The prepared TC-SiQDs also exhibit certain antibacterial ability with their minimum inhibitory concentration (MIC) against Staphylococcus aureus ( S. aureus ) at 250 μg/mL, but their inhibitory ability is effective to the growth of S. aureus biofilm only when the concentration reaches 1.3 mg/mL. TC-SiQDs-lysozyme complex were prepared, based on the effects of singlet oxygen, charge effect and lysozyme hydrolysis of peptidoglycan in the cell wall, the complex exhibits high inhibiting activity with MIC against S. aureus at 10 μg/mL and exhibits superior inhibition activity against S. aureus biofilm at a concentration of only 62.5 μg/mL. This paper provides good theoretical and practical basis for the synthesis of long-wavelength emission and ultra-bright SiQDs and their applications in detection, antibacterial and biofilm growth inhibition, and other biomedical fields.
研究领域:
材料科学
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生物医学工程
·
微生物学
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DOI
PubMed
高影响因子
Journal of Analysis and Testing
·
2021
·Vol. 5
·期 1
·19-29
·
IF 0
Liu Zhong-gang,Xiao Meng,Yang Rong-zhe,Zhou Qiao-qiao,Ye Hui-fen,Yi Chang-qing
This work reports on a smartphone-based quantitative POCT system which consists of a paper-based microarray chip, a homemade LEGO dark-box, and a smartphone installed with custom-designed FChip-APP, for on-site quantitation of Fe 3+ , Vitamin 12 (VB 12 ), and folic acid (FA). Collection and automatic analysis of fluorescence turn-off response of three kinds of fluorescent nanoprobes which are coated onto filter papers by a smartphone-based device is the principle behind this study. The POCT system exhibits good analytical performance for multiplexed analysis of Fe 3+ , VB 12 and FA with a wide linear range (Fe 3+ , 1.0–14.0 μg mL −1 ( R 2 = 0.983); VB 12 , 1.35–40.0 μg mL −1 ( R 2 = 0.991); FA, 2.0–26.0 μg mL −1 ( R 2 = 0.994)) and low detection limit (Fe 3+ , 0.35 μg mL −1 ; VB 12 , 0.47 μg mL −1 ; FA, 0.56 μg mL −1 ). The cost and weight of the whole system are ~ $220 and ~ 600 g per set, highlighting its cost-effectiveness and portability. The simple operation procedures and user-friendly interface of the FChip-APP validate the ease-of-use feature of the reported system. This versatile smartphone-based quantitative POCT system provides a cost-effective and easy-of-use method for screening the context-specific causes of anemia and thus its effective interventions in the future, especially in resource-limited areas.
研究领域:
医学检测
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生物传感
·
移动健康
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DOI
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Shen Zhang,Zhuo Wang,Yuyu Guo
Although vitamin B12 (VB12) is an essential water-soluble vitamin for the human body, the adverse reaction by excessive use is also worth noting. Therefore, it is crucial to detect the accurate concentration of VB12. In this work, biomass-based carbon dots (BCDs) were prepared by a facile one-pot hydrothermal method from black rice and urea. Based on inner filter effect and static quenching mechanism, the BCDs suggested conspicuous sensitivity and specificity for VB12 detection. The linear ranges were 0.5–100 μM and 0–50 μM under dual fluorescence and colorimetric modes, respectively. Additionally, the obvious color change by addition of VB12 in BCDs solution ensured the feasibility of visual experiments with smartphone assistance. The portable determination system maintained exceptional operability, quick analysis and excellent precision. Combined with the outstanding performance in real samples, this study offers a novel tactic for pharmaceutical analysis based on fluorescence/colorimetric/visualization modes.
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Xiaoyan Ma,Kunming Wei,Hongwei Liu,Qi Zheng,Wenqing Cao,Liang Zhang,Liangjie Tang,Stefanos Giannakis,Jing Deng,Su Jiang,Daniel L. Gallagher,Andrea M. Dietrich
Haloacetic acids (HAAs) are prevalently occurred in drinking water after disinfection by chlorine or chloramine, classified as the non-volatile and thermally stable disinfection byproducts (DBPs). To minimize the existed HAAs, catalytical electrolysis based on effective cathode was employed. Dehalogenation of HAAs were improved by vitamin B12 (VB12) coating on the stainless steel (SS), iron, copper, and aluminum electrodes. The VB12/SS composite electrode proved to be the optimal for the synergistic effect of VB12 and metal substrate on the DCAA degradation. Target chloro-, bromo-, and iodo-HAAs were catalytically decomposed, and their dehalogenation efficiency and mechanistic pathway were compared. The results show that HAAs electrolytic dehalogenation rates were significantly related with both the degree of halogenation (tri > di > mono) and the halogen atomic weight (I > Br > Cl). Dehalogenation of HAAs follows a first-order kinetic model, and kinetic variations were well described by a linear model. The logarithm base 10 of the dehalogenation rate constant k was proportional to its HAA molecular weight: log 10 (k) =0.00237 MW -0.85. The intermediate dehalogenation products were identified as less-halogenated acetic acids, and the final were acetic acid and halide ions. No mineralization occurred according to carbon conservation analysis. ESR detection and quenching experiments confirmed that HAAs dehalogenation was mainly an indirect reduction mediated by atomic H*. The synergistic effect between atomic H* and VB12 occurred through the electron transfer. This comparative study of various HAAs dehalogenation provides a mechanism of dehalogenation and a predictive linear model for electrolysis of HAAs.
研究领域:
环境科学
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化学工程
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DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 35
·19786-19790
·
IF 2.936
X. Y. Sun,M. J. Yuan,B. Liu,J. S. Shen
In this study, we constructed a new fluorescent sensing for VB12 and investigated the mechanism of vitamin B12 (VB12) quenching fluorescence of carbon dots (CDs). The fluorescence suppression is attributed to the inner filter effect (IFE) because of the overlap between UV-vis absorption spectrum of VB12 and emission/excitation spectra of CDs. This CDs-based sensor provides obvious advantages of simplicity, convenience, rapid response, high selectivity and sensitivity, which has potential application for the detection of VB12 in the medical and food industry.
研究领域:
分析化学
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生物医学工程
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纳米医学
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食品科学
·
临床检验
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DOI
高影响因子
MICROCHIMICA ACTA
·
2026
·Vol. 193
·期 9
·646
·
IF 5.4
Li Chunli,Zeng Linyun,Hong Ziyi,Xie Wenjia,Luo Tongxi,Pan Jialiang,Zhang Xinguo
Unique orange-emissive carbon dots (O-CDs) are synthesised via a solvothermal method by safranin T and 2,2’-dithiodibenzoic acid in ethanol. O-CDs show a maximum emission peak at 595 nm and a quantum yield of 81.63% when dissolved in dichloromethane. O-CDs are able to be fabricated into fluorescent hydrogels, powders and films exhibiting excellent fluorescence performance. The linear relationship is observed in the concentration range of 0-200 µM with R 2 of 0.9973, and the limit of detection is 0.19 µM. Meanwhile, this method is effectively utilized to analyze real food samples, such as egg yolk and milk, with recoveries ranging from 99.21% to 101.8%. Sensing mechanism is found to be the inner filter effect and dynamic quenching. Furthermore, O-CDs exhibit low toxicity and nice biocompatibility, facilitating the applications in bioimaging of B16F10-2 cells as well as zebrafish, and enabling VB12 detection in live cells. Therefore, the novel O-CDs developed present broad application prospects including food safety, biosensing, bioimaging and fluorescent films. Graphical
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高影响因子
LUMINESCENCE
·
2019
·Vol. 34
·期 6
·544-552
·
IF 1.691
Yao Long,Li Zhang,Ying Yu,Bixia Lin,Yujuan Cao,Manli Guo
A simple and rapid detection strategy for vitamin B 12 (VB 12 ) was established based on label-free silicon quantum dots (SiQDs); the detection mechanism was additionally investigated. SiQDs were synthesized using a one-step microwave method, and their fluorescence was stronger than that synthesized using the hydrothermal method. SiQDs fluorescence was quenched using VB 12 due to the inner filter effect (IFE), which was demonstrated using ultraviolet (UV) absorption spectra, fluorescence lifetime, transmission electron microscopy and zeta potential analysis. Subsequently, quercetin (Que) and doxorubicin (Dox) with absorption peaks that overlapped the excitation or emission peaks of SiQDs respectively were used as control groups to investigate the quenching mechanism. Results showed that quenching efficiency was related to the level of overlap between the adsorption peak of the quencher and the excitation or emission peaks of SiQDs. A greater level of overlap caused a higher quenching efficiency. Therefore, the sensitive quenching of VB 12 for SiQDs was due to the synergistic effect of the synchronous overlap between the absorption peak of VB 12 with the excitation and emission peaks of SiQDs. Fluorescence quenching efficiency increased linearly in the 0.5 to 16.0 μmol·L −1 VB 12 concentration range, and the detection limit was 158 nmol·L −1 . In addition, SiQDs were applied to determine VB 12 in tablets and human urine samples with satisfactory recoveries ranging from 97.7 to 101.1%.
研究领域:
分析化学
·
生物医学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Zexian Chen,Yaojie Zhang,Wenjuan Dong,Yang Liu,Li Zhang,Yueping Dong,Zihan Wang,Yihong Chen,Qin Hu,Chuan Dong,Xiaojuan Gong
Lignin-derived carbon dots (L-CDs) were synthesized via a simple one-step hydrothermal method using sodium lignosulfonate and polyethylene polyamine as carbon and nitrogen sources, respectively. The obtained L-CDs exhibited strong blue fluorescence, which was effectively quenched by Co(II) and VB 12 through an inner filter effect (IFE), accompanied by distinct color changes under both visible and ultraviolet light. Taking advantage of this property, a smartphone-assisted visual sensing strategy was developed for the rapid and portable detection of Co(II) and VB 12 with high selectivity and sensitivity. The detection limits were as low as 0.17 μM for Co(II) and 0.03 μM for VB 12 . Furthermore, the proposed sensing platform was successfully applied to the determination of Co(II) in environmental water samples and VB 12 in pharmaceutical formulations. This work not only demonstrates a novel design of carbon dot-based dual-mode sensors but also highlights the value-added utilization of lignin in analytical applications.
研究领域:
分析化学
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环境监测
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药物分析
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纳米生物技术
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传感器开发
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食品安全
·
转化医学
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2019
·Vol. 136
·期 45
·48179
·
IF 2.188
Miao Wang,Yuan-Wei Huang,Yan Chen,Xi Yan,Wen-Yan Xu,Wan-Zhong Lang
Poly(vinylidene fluoride) (PVDF) membranes without any additives were fabricated by a vapor-induced phase separation method and applied to the adsorption separation of vitamin B12 (VB12) from aqueous solution. The effects of preparation conditions including PVDF concentration in dope, vapor humidity, and exposure time on the morphologies, mechanical strength, adsorption, and permeation performances are investigated in detail. The results indicate that the PVDF membranes have a uniform spongy structure without macrovoids. The lower polymer concentration, relatively higher humidity, and longer exposure time easily leads to the formation of larger polymer nodules in the membrane matrix and lower mechanical strength of membranes. The resulted PVDF membranes exhibit superior separation efficiency for both static and dynamic adsorption for VB12. In the ultrafiltration (adsorption)-regeneration cycles, the permeation flux remains above 2500 L m −2 h −1 bar −1 and the VB12 removal ratio is above 92% after six cycles. The prepared PVDF membranes show superior adsorption capacities to other traditional adsorbents (such as activated carbons, nanoclay, etc.) for VB12 removal. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136 , 48179.
研究领域:
材料科学
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环境工程
·
生物技术
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DOI
高影响因子
ACS Nano
·
2022
·
IF 18.027
Han Wang,Xueqi Wang,Jing Cheng
Nitrite plays a critical role in a variety of physiological processes and maintaining the nitrite level in an appropriate range is vital to keep healthy. Current nitrite analysis methods lack sensitivity and require tedious operations, which could not meet the need of point-of-care (POC) nitrite detection in precision medicine. Here we present a cyanocobalamin (VB12) bionic enzyme-assisted ion-selective amperometric biosensor based on 3D porous conductive matrix (PCM), which can facilitate rapid and accurate POC nitrite monitoring in complex biofluids. The experimental findings quantitatively demonstrate that the biosensor has a sensitivity of 64.08 μA/(mM·cm2), a wide linear range of 0.025–45 mM, and low limit of detection of 1 nM. Moreover, the developed VB12/BSA-PCM biosensor shows outstanding stability after 21 days with 2% decline in current signal, and high repeatability between batches with RSD of only 1.29%. Real salivary nitrite detection has been evaluated, and the results match well with the commercial nitrite analyzer. Thus, the bionic enzyme-assisted ion-selective amperometric biosensor proposed herein has potential utility as an affordable tool for POC detection and home-based healthcare.
研究领域:
分析化学
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生物医学工程
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即时诊断
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生物传感器
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电化学
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DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2021
·
IF 8.742
Ze-Lin Qiu,Wen-Han Yu,Yu-Jie Shen,Bao-Ku Zhu,Li-Feng Fang
High-efficient separation of molecules is of great significance in food, biotechnology, pharmaceutical and dairy industries. To this end, cationic hyperbranched poly (amido-amine)s (HBPs) with different numbers of quaternary ammonium groups were employed as a new kind of 3D spherical macromonomers to fabricate nanofiltration (NF) membranes, through interfacial polymerization (IP) between cationic HBPs and trimesoyl chloride (TMC) on a polyvinyl chloride ultrafiltration (PVC-UF) hollow fiber membrane surface. Apart from the inner voids inside cationic HBP molecules for fast transport of water molecules, sizes and charge properties of the outer voids between cationic HBP molecules can be modulated by changing the numbers of quaternary ammonium groups on cationic HBP molecule surfaces for enhancing both high permeability and selectivity (water permeance and MgCl 2 rejection rate of the resultant NF membrane are up to 310.5 L m −2 h −1 MPa −1 and 92.0% respectively). Based on the separation mechanism of combined steric and Donnan effects, the resultant NF membrane exhibits ultrahigh retentions to molecules with high molecular weights (MWs) or positive charges, thus molecular-level selectivity to VB12/VB2 (neutral molecules with distinct MWs) or VB1/VB2 (cationic/neutral molecules with similar MWs) can be achieved.
研究领域:
化学工程
·
材料科学
·
膜技术
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生物技术
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药学
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食品科学
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产品关联
DOI
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Xuemei Chen,Yi Ge,Xiaoyong Zhu,Kaige Chen,Jian Li,Maoshen Chen,Ling Chen,Bo Hu,Xiaoshu Tang,Yun Ma,Fei Liu
Vitamin B 12 (VB 12 ) is photolabile and exhibits low oral bioavailability. Its instability in the gastrointestinal tract, along with limited intrinsic-factor-mediated absorption, restricts its application in functional foods. To address these limitations, we proposed a novel strategy that utilized hydrogenated palm oil (HPO) and soybean oil (SBO) lipid blends (HPO/SBO) to improve the photostability and tailor digestive release of VB 12 encapsulated in freeze-dried W/O/W microcapsules. Adjusting the blend ratio modulates the crystallinity and packing density of the lipid matrix, thereby controlling the photostability and digestion behavior of encapsulated VB 12 . The formulation was subsequently applied to the gummy candies. Results indicated that increasing HPO proportion to 60% promoted a denser microcapsule network and reduced water activity, consequently improving VB 12 light retention from 26% to approximately 60%. Notably, this formulation ratio yielded favorable low gastric release (<50%) and efficient intestinal release (nearly 100%), resolving the typical trade-off between gastric protection and intestinal release. When incorporated into gummy candies, this formulation also demonstrated superior light protection and release balance with good system adaptability. Overall, HPO/SBO (60:40) serves as an effective structuring agent to stabilize VB 12 and tune its release through crystalline network regulation. This strategy holds great potential for designing high-performance VB 12 -fortified foods.
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高影响因子
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