BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Hui Zhang,Meixing Li,Caihong Li,Zhihui Guo,Huilei Dong,Ping Wu,Chenxin Cai
The expression profile of vascular endothelial growth factor (VEGF) is highly correlated with the occurrence and development of cancer. This work reports an electrochemiluminescence (ECL) approach for highly sensitive detection of VEGF 165 . This approach comprises aptamer–target recognition, T7 exonuclease (T7 Exo)-assisted cycling signal amplification and efficient quenching of ECL of CdS:Eu nanocrystals (NCs) by using DNAzyme. In this assay, CdS:Eu NCs were used as the ECL substrate, A guanine (G)-rich single-stranded DNA (ssDNA) sequence and VEGF 165 aptamer were co-immobilized on the surface of the CdS:Eu NCs modified glassy carbon electrode. After recognition and binding to VEGF 165 , the aptamer moved away from the electrode surface and induced the proposed cyclic cleavage of the target DNA with T7 Exo. A large amount of G-rich ssDNA was released on the CdS:Eu film and folded into G-quadruplex/hemin DNAzyme in the presence of hemin and K + , consequently decreasing the ECL intensity of CdS:Eu. A good linearity was obtained for VEGF 165 detection within the range of 1 pM to 20 nM with a detection limit of 0.2 pM. This assay could be a universal and promising protocol for detection of various biomarkers for early clinical diagnosis.
研究领域:
生物化学
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医学诊断
·
纳米技术
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DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Bowen Xiong,Xiang Li,Yuting Zhang,Changling Yan,Gongke Wang
The overexpression of vascular endothelial growth factor 165 (VEGF 165 ) in cancer cells plays a pivotal role in promoting tumor metastasis by facilitating their excessively rapid proliferation and division. Hence, the development of analytical methods possessing high sensitivity and resistance to interference is imperative for the detection of VEGF 165 . Various types of aptasensors have been devised for VEGF 165 detection; however, the performance of these biosensors can be influenced by non-target signals caused by conformational changes in unbound aptamers. The paper shows the creation of a precise and sensitive fluorescence biosensor designed to detect VEGF 165 by using a VEGF 165 -specific split aptamer. Additionally, this biosensor employs nicking enzyme-assisted DNA walker coupling with CRISPR-Cas12a to achieve dual-signal amplification. The VEGF 165 calibration curve shows a detection limit of 268 fM and has a broad linear range from 5 to 4000 nM. The fluorometric biosensor was utilized to detect VEGF 165 in human serum and cellular homogenate samples, yielding good outcomes. The innovative design serves as proof of concept and demonstrates significant potential in detecting various targets.
研究领域:
生物技术
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分子生物学
·
医学诊断
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PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Huimei Da,Hongyan Liu,Yingning Zheng,Ruo Yuan,Yaqin Chai
We developed a novel “signal-off” photoelectrochemical (PEC) aptasensor based on an aptamer bridged DNA network structure for the sensitive detection of vascular endothelial growth factor (VEGF 165 ), using g-C 3 N 4 as photoactive material. The DNA network provides an excellent platform for the immobilization of methylene blue (MB), which can facilitate the electron transport through the DNA helix structure and suppress the recombination of electron-hole pairs generated by g-C 3 N 4 . In the presence of the target VEGF 165 , the DNA network can be destroyed adequately by the recognition between VEGF 165 and the aptamer, resulting in the release of MB. Therefore, the originally enhanced electron transfer process could be inhibited, leading to a remarkable decrease of the photocurrent . A good linear relationship between the PEC signal and the logarithm of VEGF 165 concentration over the range of 100 fM to 10 nM with a detection limit of 30 fM can be obtained. Our concept can be easily extended to develop aptasensors for the sensitive detection of different targets by triggering the release of the payloads from their corresponding aptamer bridged DNA networks.
研究领域:
生物化学
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分析化学
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生物医学工程
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 13
·3073-3081
·
IF 3.637
Cheng Jin-long,Liu Xing-Pei,Chen Jing-Shuai,Mao Chang-jie,Jin Bao-Kang
In this work, an electrochemiluminescence (ECL) biosensor was fabricated for the selective detection of vascular endothelial growth factor (VEGF 165 ). g-C 3 N 4 /PDDA/CdSe nanocomposites were used as the ECL substrate. Then, DNA labeled at the 5′ end with amino groups (DNA 1 ) was immobilized on the surface of g-C 3 N 4 /PDDA/CdSe nanocomposite-modified glassy carbon electrode (GCE) by amido linkage. AuNP-labeled target DNA (Au-DNA 2 ) could hybridize with DNA 1 to form a double strand. The ECL of the g-C 3 N 4 /PDDA/CdSe nanocomposite was efficiently quenched due to the resonance energy transfer between CdSe QDs and Au NPs. After VEGF 165 was recognized and bound by Au-DNA 2 , the double helix was disrupted, and the energy transfer was broken. In this case, Au-DNA 2 was released from the electrode surface, and the ECL intensity recovered to a higher level. Under optimal conditions, this ECL biosensor possesses excellent selectivity, accuracy, and stability for VEGF 165 detection in a linear range of 2 pg mL −1 to 2 ng mL −1 with a detection limit of 0.68 pg mL −1 . In addition, this assay has been successfully applied to the determination of VEGF 165 in serum samples.
Schematic representation of the electrochemiluminescence sensor based on a g-C3N4/PDDA/CdSe nanocomposite, which can be determined in the concentration of vascular endothelial growth factor in serum.
研究领域:
生物化学
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分析化学
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纳米技术
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生物传感器
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PubMed
高影响因子
TALANTA
·
2017
·
IF 4.162
Siwen Shan,Ziyi He,Sifeng Mao,Mingsha Jie,Linglu Yi,Jin-Ming Lin
In this work, we have developed a sensitive and selective chemiluminescence (CL) assay for vascular endothelial growth factor (VEGF165) quantitative detection based on two specific VEGF165 binding aptamers (Apt). VEGF is a predominant biomarker in cancer angiogenesis, and sensitive detection method of VEGF are highly demanded for both academic study and clinical diagnosis of multiple cancers. In our experiment, VEGF165 was captured in a sandwich structure assembled by two binding aptamers, one capture aptamer was immobilized on streptavidin-coated magnetic beads (MBs) and another VEGF-binding aptamer was labeled by biotin for further phosphatase conjunction. After Apt-VEGF-Apt sandwich was formed on MBs surface, alkaline phosphatase (ALP) was modified to the second aptamer to catalyze CL reaction. By applying 4-methoxy-4-(3-phosphatephenyl)-spiro-(1,2-dioxetane-3,2-adamantane) (AMPPD) as CL substrate, strong signal intensity was achieved. VEGF165 content as low as 1 ng/mL was detected in standard spiked samples by our assay, and linear range of working curve was confirmed from 1 to 20 ng/mL. Then our method was successfully applied for cell culture medium analysis and on-chip hypoxic HepG2-HUVEC co-culture model study with excellent accuracy equal to ELISA Kit. Our developed assay demonstrated an outstanding performance in VEGF165 quantification and may be further extended to clinical testing of important biomarkers as well as probing microchip cell culture model.
研究领域:
癌症生物学
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分子诊断学
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分析化学
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生物医学工程
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PubMed
高影响因子
Biomedical Materials
·
2025
·Vol. 20
·期 5
·055009
·
IF 3.7
Yunguo Liao,Hong Hu,Ziyu Tang,Dan Deng,Chao Li
This study presents a novel electrochemical aptasensor utilizing polydopamine–graphene (PDA–G) nanocomposite for VEGF165 detection. The PDA–G nanocomposite was synthesized through a one-step self-polymerization process and characterized by SEM, XPS, and FTIR, confirming successful PDA coating on graphene sheets with 22.3% C–N bond incorporation. Electrochemical characterization confirmed stepwise increases in charge transfer resistance (Rct), and quantitative detection was based on ΔRct (the change in Rct before vs. after VEGF165 binding). Under optimized conditions (pH 7.4, 37 °C, 30 min incubation), the sensor demonstrated dual-linear response regions with high sensitivity. Analysis of clinical samples showed minimal matrix effects with a 1:4 dilution ratio, and endogenous VEGF165 levels in healthy donor serum were determined to be 28.4 ± 3.2 pg ml−1. The method’s practicality was validated through comprehensive interference studies, with common serum proteins generating relative responses below 5.2% compared to the target.
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高影响因子
LAB ON A CHIP
·
2023
·Vol. 23
·期 10
·2469-2476
·
IF 6.1
Xinyu He,Junyan Xu,Xiaoli Wang,Chuang Ge,Shunbo Li,Li Wang,Yi Xu
In this paper, a multifunctional microfluidic chip integrated with a centrifugal separation zone, aqueous two-phase system (ATPS) mixing zone and enrichment detection zone was proposed and fabricated. An automatic and efficient separation and quantitative analysis method for vascular endothelial growth factor 165 (VEGF165) in whole blood samples was established with the designed microfluidic chip. A blood sample was divided into blood cells and plasma in the centrifugation zone. In the ATPS mixing zone, plasma was mixed with PEG/KH2PO4 aqueous two-phase solution containing Apt–Au NP nanoprobes. In the enrichment detection zone, the mixture was separated on CN140 modified with a ZnO NP–anti VEGF165 nanostructure. The VEGF165 captured by Apt–Au NPs was distributed in the PEG phase, concentrated at the front of CN140 and combined with anti-VEGF165 to form a sandwich structure. The sensitive detection of VEGF165 was achieved through fluorescence resonance energy transfer between rhodamine B and Au NPs on the nanoprobe. Under the optimized rotation program, capillary and centrifugal forces propelled the fluid in the whole process of pretreatment and detection. The detection linear range was between 1 pg mL−1 and 50 ng mL−1, the detection limit of VEGF165 in blood was 0.22 pg mL−1 and the enrichment efficiency was 983. It was illustrated that a convenient and reliable way for detection of tumor markers based on the multifunctional microfluidic chip was provided and it has a potential value for early screening and prognosis of clinical cancer.
研究领域:
生物医学工程
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分析化学
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癌症研究
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分子生物学
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PubMed
高影响因子
ACS Sensors
·
2022
·
IF 9.618
Xinyu He,Xiaoli Wang,Chuang Ge,Shunbo Li,Li Wang,Yi Xu
For the rapid and sensitive detection of vascular endothelial growth factor 165 (VEGF165) in clinical blood samples, a microfluidic sensing chip that integrates a centrifugal separation pretreatment unit and a composite nanosensing film was proposed in this paper. An efficient sensing strategy and method was established. The blood sample was first separated and extracted by centrifugal force on the centrifugal microfluidic chip within 5 min after injection. The separated plasma can be automatically transferred through the designed microchannels to the detection area integrated electrodes for subsequent differential pulse voltammetric detection. The Au NPs/MCH/Apt2 sensing film was constructed on the surface of the Au working electrode. A sandwich sensing strategy based on “double aptamers” and “nanoprobe” for VEGF165 detection was established, by which the synthetic Apt1/PThi/Au NP nanoprobe was applied to capture VEGF165 in plasma and bind to the sensing film. By this method, the detection limit of VEGF165 in whole blood was 0.67 pg/mL and the linear range was between 1 pg and 10 ng, which met the needs of clinical VEGF165 detection. It was illustrated that the proposed methodology based on the centrifugal microfluidic chip had potential application prospects in the development of the point-of-care testing fields.
研究领域:
生物医学工程
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分析化学
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即时诊断
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微流控技术
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生物传感器
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DOI
PubMed
高影响因子
Biofabrication
·
2025
·Vol. 17
·期 4
·045013
·
IF 8
Wenxing Han,Hongguang Chen,Huan Chen,Yiran Xi,Dezhi Huang,Shanshan Yong,Yuanbo Zhou,Hui Liu,Chunli Zhang
This study aimed to improve the efficiency of decellularization and enhance the functional properties of vascular grafts to optimize their application in vascular repair. Rabbit abdominal aortas were used as the decellularization target, and ultrasound-assisted decellularization was performed using intermittent ultrasound at 100 W power, 20 kHz frequency, and 4 °C. Rabbit abdominal aortas were subjected to three different decellularization techniques. Based on comparative evaluation, ultrasound-assisted decellularization was implemented to enhance cell removal efficiency. In addition, dual-factor surface modification was performed using sodium heparin (HEP) and vascular endothelial growth factor 165 (VEGF165) to investigate anticoagulant and endothelialization potential. Ultrasound optimization enhanced decellularization efficiency by 1.5 times, increased matrix integrity to 85%, and decreased chemical residues by 30%. Dual-factor functionalization with HEP and VEGF165 improved anticoagulant properties by 40%, prolonged thrombus formation time by 45%, and enhanced endothelialization by 68%. In vivo animal studies demonstrated a 93% blood flow patency rate post-implantation, with superior tissue repair compared to the control group. This study presents an innovative approach that integrates ultrasound optimization and functional modification, addressing the limitations of traditional decellularization methods. It offers a high-performance, low-toxicity strategy for developing vascular grafts with significant clinical potential, particularly for small-diameter vascular applications.
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高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Tianxiang Wei,Tingting Dong,Hong Xing,Ying Liu,Zhihui Dai
To achieve high selectivity and sensitivity simultaneously in an electrochemical biosensing platform, cucurbituril and azide cofunctionalized graphene oxide, a new functional nanomaterial that acts as a go-between to connect the recognition element with amplified signal architecture, is developed in this work. The cucurbituril and azide cofunctionalized graphene oxide features a high specific surface area with abundant levels of the two types of functional groups. Specifically, it emerges as a powerful tool to link recognition elements with simplicity, high yield, rapidity, and highly selective reactivity through azide-alkynyl click chemistry. Moreover, it possesses many host molecules to interact with guest molecules (also signal molecules)-grafted branched ethylene imine polymer, through which the detection sensitivity can be greatly improved. Together with electro-click technology, a highly controllable, selective, and sensitive biosensing platform can be easily created. For VEGF165 protein detection, the electro-click assay has high selectivity and sensitivity; a dynamic detection range from 10 fg mL–1 to 1 ng mL–1 with a detection limit of 8 fg mL–1 was achieved. The electro-click biosensing strategy based on cucurbituril and azide cofunctionalized graphene oxide would have great promise for other target analytes with a broad range of applications.
研究领域:
生物传感器
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纳米材料科学
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分析化学
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DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Yanling Zhang,Min Liu,Shuai Pan,Lulu Yu,Shaoshuai Zhang,Ruijiang Liu
Based on magnetic Fe 3 O 4 /Fe 2 O 3 @Au nanoparticles (Fe 3 O 4 /Fe 2 O 3 @Au NPs), to detect an important tumor screening marker, vascular endothelial growth factor 165 (VEGF 165 ), a label-free electrochemical aptasensor was constructed. Magnetic glassy carbon electrode (MGCE) after Fe 3 O 4 /Fe 2 O 3 @Au NPs surface modification by a magnetic-induced self-assembly technique was the platform. Fe 3 O 4 /Fe 2 O 3 @Au NPs were used as the signal amplifier to enhance electron transfer, which was essential for the detection of trace amounts of biological analytes, and were prepared via heating hydrolyzation-combustion calcination and trisodium citrate reduction. Meanwhile, aptamers bound to the as-prepared nanomaterial via Au-s bonds and specifically recognized VEGF 165. The electrochemical properties of the aptasensor were evaluated with [Fe(CN) 6 ] 3−/4 − as redox probes. When in optimal detection conditions, an excellent linear relationship (between 0.01 and 10 pg mL −1 , R 2 = 0.9973) was obtained for the electrochemical aptasensor. The limit of detection (LOD) and the limit of quantitation (LOQ) of the electrode were 0.01 and 0.03 pg mL −1 . This proposed aptasensor exhibited favourable selectivity, stability, reproducibility, and regenerability. The results demonstrated that the sensor was promising to provide an efficient, accurate, and low-cost auxiliary detection method for the early screening of VEGF 165 -related diseases, which had important clinical practical value for the research of human precision medicine.
研究领域:
分析化学
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生物传感器
·
纳米技术
·
癌症研究
·
电化学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 3
·1-12
·
IF 5.3
Zhao Chaoshan,Gan Minshan,Jiang Zhuoya,Lv Zilan,Feng Wei,Wang Li,Li Shunbo
A novel anisotropic nanoparticle structure consisting of a single gold nanorod and nanospheres is designed. The optical properties, especially the scattering under polarized light, are studied. The signal readout is conducted using dark-field microimaging techniques in a microfluidic chip. The formation of this novel structure is induced by the intermediate biomolecules. Therefore, it demonstrates potential applications in the ultrasensitive detection of biomarkers. As an example, the detection of vascular endothelial growth factor (VEGF165) is demonstrated, and the specificity is also investigated. This unique approach not only effectively reduces background interference but also provides a new approach for accurate sensing of targeted tumor markers. Graphical
研究领域:
纳米生物学
·
生物医学工程
·
光学传感
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PubMed
高影响因子
危险品化学品经营许可证（带存储）