BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
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2023
·Vol. 110
·期 2
·1-8
·
IF 2.7
Wu Shaoxiong,Wang Lianyuan,Zhu Haiyan,Yuan Ling,Li Jian,Ge Liang,Zhang Hongpeng,Miao Ting,Cheng Zhenxing
The analysis of nerve agents is the focus of chemical warfare agent determination because of their extreme toxicity. A classical chemical colorimetric method, namely, the Schoenemann reaction, has been developed to detect G agents; however, it has not been utilized for VX analysis mainly because of its low peroxyhydrolysis rate. In this study, based on the mechanism of the Schoenemann reaction, a novel rapid quantitative determination method for VX was developed by optimizing the reaction conditions, such as concentrations of peroxide and the indicator, temperature, and reaction time. Using 2 ml 0.5 wt% sodium perborate as the peroxide source, 1 ml 0.1 wt% benzidine hydrochloride as the indicator, and 1 ml acetone as the co-solvent, VX and GD in ethanol or water solutions could be quantitatively analyzed within 15 min at 60°C. Further experiments based on 31 P NMR spectroscopy confirmed the existence of a peroxyphosphate intermediate during the GD assay. This quantitative colorimetry system for VX and GD analysis can be developed as a portable device for the water samples in fieldwork applications.
研究领域:
化学
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环境科学
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DOI
PubMed
高影响因子
PHARMACOLOGICAL RESEARCH
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2022
·
IF 10.334
Bei Yan,Panpan Liu,Xiaoqin Yi,Jie Li,Nian Liu,Wu Zhu,Yehong Kuang,Xiang Chen,Cong Peng
Background Psoriasis is a chronic inflammatory disease , with lesions mainly manifesting as scaly erythematous plaques. The mild or moderate of psoriasis is the main type of patients in hospital, and topical application remains the preferred treatment option for psoriasis therapy, therefore, the development of novel topical agents has an essential role in psoriasis therapy. Objective To identify potential drugs for psoriasis topical treatment. Methods We performed drug screening by Imiquimod (IMQ)-induced psoriatic like inflammation in mouse model, followed mouse epidermis by RNA-seq to find the key molecules affecting the drug. The qRT-PCR, WB were performed to test mRNA and protein expression, and Chip assay had been conducted to examine Stat3 bound to promoter of FABP5. Results In this study, we identified VX-509, which topical application significantly attenuated IMQ-induced psoriatic like inflammation in mouse model. And then, we verified Epidermal Fatty acid binding protein (E-FABP/FABP5) was significantly decreased in VX-509 treated mouse epidermis by RNA-seq. FABP5 is a key molecule in lipid metabolism, administration of FABP5 inhibitor or knock down of FABP5 expression remarkably abrogated psoriatic inflammation as well as lipid metabolism. Mechanistically, our finding showed that VX-509 blocked IL-22 induced signaling pathway, particular in activation of Stat3. Furthermore, we identified Stat3 is a transcriptional factor associated with FABP5 promoters and VX-509 treatment remarkably attenuated IL-22-induced FABP5 expression through Stat3 in KCs. Conclusions This study demonstrated administration of VX-509 is a potential promising topical drug for treatment of psoriasis, FABP5 is a critical targeted molecule in psoriasis therapy.
研究领域:
皮肤科
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药理学
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分子生物学
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炎症
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DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
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2023
·
IF 2.9
Qiao Lv,Pei-Yu Jiang,Jun-Mei Xia,Long-Hui Liang,Bo Chen,Yang Yang,Long Yan,Hui-Lan Yu,Chang-Cai Liu
The retrospective detection of organophosphorus nerve agents (OPNAs) exposure has been achieved by the off-site analysis of OPNA-human serum albumin (HSA) adducts using mass spectrometry-based detection approaches. However, few specific methods are accessible for on-site detection. To address this, a novel immunofluorescence microfluidic chip (IFMC) testing system combining europium chelated microparticle (EuCM) with self-driven microfluidic chip assay has been established to unambiguously determine soman (GD) and VX exposure within 20 min, respectively. The detection system was based on the principle of indirect competitive enzyme-linked immunosorbent assay. The specific monoclonal antibodies that respectively recognized the phosphonylated tyrosine 411 of GD-HSA and VX-HSA adducts were labeled by EuCM to capture corresponding adducts in the exposed samples. The phosphonylated peptides in the test line and goat- anti -rabbit antibody in the control line were utilized to bind the EuCM-labeled antibodies for signal exhibition. The developed IFMC chip could discriminatively detect exposed HSA adducts with high specificity, demonstrating a low limit of detection at exposure concentrations of 0.5 × 10 −6 mol/L VX and 1.0 × 10 −6 mol/L GD. The exposed serum samples can be qualitatively detected following an additional pretreatment procedure. This is a novel rapid detection system capable of discriminating GD and VX exposure, providing an alternative method for rapidly identifying OPNA exposure.
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PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 56
·35245-35257
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IF 3.361
Xingqi Huang,Ting Zhao,Hongpeng Zhang,Chunxiao Yan,Jiulong Sha,Huamin Tang,Haiyan Zhu,Yue Wu
Traditional air filter papers can only filter toxic aerosols without the function of decontamination. If the poison stagnating in the paper is desorbed, it may pose a secondary threat to personnel and make it more difficult to dispose of the scrapped paper. Using an alkali-free glass fiber as the base material and zirconium hydroxide as the decontaminant, a self-decontaminating air filter paper that can degrade HD and VX simultaneously was successfully prepared by an intra-pulp addition method, with high filtration efficiency, low pressure drop and moderate tensile strength. The physicochemical properties were characterized by FE-SEM, EDX, XRD and TGA, and the results indicated that Zr(OH)4 was dispersed uniformly in the paper and filled in the interstices of the glass fiber. The preparation of the composite material had no impact on the structure of fibers and Zr(OH)4. The preparation technology of the self-decontaminating air filter paper was optimized. It was found that the paper with a fiber grammage of 50 g m−2, the adhesive of 2% and a Zr(OH)4 retention rate of 175.0 wt% could completely degrade HD and VX, whose conversion rate exceeded 99.0%, and had a tensile strength of 0.1193 kN m−1, a filtration efficiency of 99.995%, and a pressure drop of 313.6 Pa. Using GC-MS to detect the decontamination products, it was speculated that HD mainly underwent hydrolysis and elimination reactions, VX mainly underwent hydrolysis and polymerization reactions, and their products were non-toxic or low-toxic. The reaction kinetics of HD and VX on the paper was investigated and the half-lives were 2.6 h and 16.2 min, respectively, which demonstrated an outstanding degradation performance. This work manifested for the first time that the air filter paper can be optimized as an efficient self-decontaminating material, which will open up new possibilities for the design and manufacture of multifunctional protective materials.
研究领域:
材料科学
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环境科学
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DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2021
·Vol. 32
·期 26
·265101
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IF 3.874
Qingqing Dai,Wei Jiang,Hu Liu,Xin Qing,Guobin Wang,Fan Huang,Zhilai Yang,Chunhui Wang,Erwei Gu,Hongchuan Zhao,Jiqian Zhang,Xuesheng Liu
The aim of this study is to evaluate the effect of rare earth upconversion nanoparticles (UCNs) on hepatic ischemia reperfusion injury (IRI) and explore its possible mechanism. Hepatic IRI seriously affects the prognosis of patients undergoing liver surgery. Liver-resident Kupffer cells have been reported to promote IRI. Nanomedicines are known to be effective in the treatment of liver diseases, however, Kupffer cell-targeting nanomedicines for the treatment of IRI are yet to be developed. As potential bioimaging nanomaterials, UCNs have been found to specifically deplete Kupffer cells, but the underlying mechanism is unknown. In this study, we found that UCNs specifically depleted Kupffer cells by pyroptosis, while the co-administration of the caspase-1 inhibitor VX-765 rescued the UCN-induced Kupffer cell pyroptosis in mice. Furthermore, the pre-depletion of Kupffer cells by the UCNs significantly suppressed the release of inflammatory cytokines and effectively improved hepatic IRI. The rescue of the pyroptosis of the Kupffer cells by VX-765 abrogated the protective effect of UCNs on the liver. These results suggest that UCNs are highly promising for the development of Kupffer cell-targeting nanomedicines for intraoperative liver protection.
研究领域:
纳米技术
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肝病学
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药理学
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DOI
PubMed
高影响因子
AIP Advances
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2022
·Vol. 12
·期 3
·
IF 1.697
Wu Yao,Kong Lingce,Zhang Xiuling,Guo Yueting,Sun Yaxin,Zhao Chonglin,Chen Wenming,Zuo Yanjun,Li Congju
Protection against harmful chemical compounds is a major social concern, especially chemical warfare agents (CWAs) that are easy to synthesize and can cause mass casualties. The metal oxides with large surface area, porous structures, and a large number of active sites have shown outstanding performance for the adsorption and decontamination of CWAs. However, single-component metal oxides show a lower degradation rate for chemical warfare agents. Here, we demonstrate a convenient homogeneous hydrolysis method for the synthesis of mesoporous manganese-based multi-component metal oxides. Our strategy enables Ce or/and Zr to be doped into the δ-MnO 2 structure during the forming process, remarkably enlarging the surface area and providing rich active sites for CWA catalysis. The as-synthesized multi-component metal oxides exhibit excellent performance for the CWA degradation. It is surprising to find that the best degree of removal of mustard gas (HD), soman (GD), and the VX nerve agent (VX) is 90.60%, 87.32%, and 100%, respectively, reaction with multi-component samples in 4 h, 2.92-fold increase to HD, 8.28-fold increase to GD, and 1.67-fold increase to VX contrast with undoped δ-MnO 2 . This work may provide an avenue for developing new generation chemical warfare agent decontamination materials and other catalysts.
研究领域:
材料科学
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化学工程
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环境科学
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DOI
高影响因子
ACS Omega
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2022
·
IF 4.132
Guoqing Wu,Dongjiu Zhang,Wei Xu,Hongjun Zhang,Likun Chen,Yongchao Zheng,Yi Xin,Hong Li,Yan Cui
Chemical warfare agents (CWAs) can be absorbed in polymeric coatings through absorption and permeation, thus presenting a lethal touch and vapor hazards to people. Developing a highly impermeable polymer coating against CWAs, especially against organophosphate CWAs (OPs), is challenging and desirable. Herein, fluorinated epoxy (F-EP) and epoxy (EP) coatings with different cross-link densities were prepared to resist OPs. The effects of the polymer coating structure, including cross-link density, chemical composition and free volume, on the chemical resistance to dimethyl methylphosphonate (DMMP, Soman simulant) were investigated in detail. Meanwhile, the chemical resistance to Soman and VX was examined. The results reveal that the cross-link density is a critical factor in determining the chemical resistance of the coatings. Highly cross-linked EP and F-EP coatings with dense and solid cross-linked networks can fully bar DMMP and OPs permeation during the test time. At low or medium cross-link densities, the EP coating with a lower retention of DMMP exhibited a higher resistance than the F-EP coating due to the lower interaction with DMMP and smaller free-volume holes and lower relative fractional free volume. These results suggest that increasing the cross-link density is a reasonable approach to control the chemical resistance of polymer networks against OPs.
研究领域:
材料科学
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高分子化学
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化学工程
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环境科学
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DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 36
·22125-22130
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IF 3.361
Chuan Zhou,Shouxin Zhang,Hongjie Pan,Guang Yang,Lingyun Wang,Cheng-an Tao,Heguo Li
The potential threat that has originated from chemical warfare agents (CWAs) has promoted the development of advanced materials to enhance the protection of civilian and military personnel. Zr-based metal–organic frameworks (Zr-MOFs) have recently been demonstrated as excellent catalysts for decomposing CWAs, but challenges of integrating the microcrystalline powders of Zr-MOFs into monoliths still remain. Herein, we report hierarchically porous monolithic UiO-66-X xerogels for the destruction of CWAs. We found that the UiO-66-NH2 xerogel with a larger pore size and a higher surface area than the UiO-66-NH2 powder possessed better degradability of 2-chloroethyl ethyl sulfide (2-CEES), which is a sulfur mustard simulant. These UiO-66-X xerogels exhibit outstanding performance for decomposing CWAs. The half-lives of vesicant agent sulfur mustard (HD) and nerve agent O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothioate (VX) are as short as 14.4 min and 1.5 min, respectively. This work is, to the best of our knowledge, the first report on macroscopic monolithic UiO-66-X xerogels for ultrafast decomposition of CWAs.
研究领域:
材料科学
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化学工程
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）