FOOD CHEMISTRY
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2025
·
IF 9.8
Aiping Li,Chen Wang,Yunfeng Chai,Zhiming Huang,Binbin Yao,Chengyin Lu,Miao Yu,Hongping Chen
Validamycin A (VA) and spinosyn A (SA) have been utilized as antibiotic pesticides in tea plantations for the control of pests and diseases. Both pesticides pose significant risks to human health and have been listed as key controlled new pollutants in China. So far, there have been few reports for the risk assessment of antibiotic pesticides in tea. In this study, a hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC-MS/MS) method was developed for the simultaneous detection of VA and SA residues in tea. Method validation demonstrated good accuracy and precision. During tea planting, SA and VA in tea shoots degraded fast, with the half-life of 0.36 to 0.43 days for SA and 0.72 to 0.92 days for VA. SA and VA in tea shoots degraded fast initially and then followed by a slower phase. Both SA and VA degraded rapidly during the withering and drying stages. Compared to green tea, the degradation rates of both pesticides were faster during black tea manufacturing. Transfer rates of VA and SA from tea into infusions had transfer rates of 76.7 % to 99.5 % and 1.5 % to 2.7 %, respectively. The chronic risk quotient (RQc) < 1 for both antibiotic pesticides indicated negligible consumer risk from tea.
研究领域:
农业科学
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食品科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Insects
·
2021
·Vol. 12
·期 4
·370
·
IF 2.769
Zuo-min Shao,Jian-hao Ding,De-lei Jiang,Zhi-xiang Liu,Yi-jiangcheng Li,Jiao Wang,Jun Wang,Sheng Sheng,Fu-an Wu
Simple SummarySericulture has always been threatened byGlyphodes pyloalisWalker (G. pyloalis). Trehalase is an essential enzyme in chitin metabolism and energy supply. In this study, two trehalase genes inG. pyloalis(GpTre1andGpTre2) were identified and functionally analyzed. Knockdown of the two genes led to the significant downregulation of chitin metabolism pathway-related genes, the difficulty in molting of larvae, and the deformation of adult wings. Moreover, the trehalase inhibitor, Validamycin A, treatment increasedGpTre1andGpTre2expression and affected the expressions of chitin metabolism pathway-related genes. The inhibitor also caused a significantly increased cumulative mortality of larvae. The results suggested thatGpTre1andGpTre2played a vital role onG. pyloalisdevelopment, which could be useful for providing information for insect pest control in the future.AbstractGlyphodes pyloalisWalker (G. pyloalis) is a serious pest on mulberry. Due to the increasing pesticide resistance, the development of new and effective environmental methods to controlG. pyloalisis needed. Trehalase is an essential enzyme in trehalose hydrolysis and energy supply, and it has been considered a promising target for insect pest control. However, the specific function oftrehalaseinG. pyloalishas not been reported. In this study, two trehalase genes (GpTre1andGpTre2) were identified from our previous transcriptome database. The functions of thetrehalasein chitin metabolism were studied by injecting larvae with dsRNAs and trehalase inhibitor, Validamycin A. The open reading frames (ORFs) of GpTre1 and GpTre2 were 1,704 bp and 1,869 bp, which encoded 567 and 622 amino acid residues, respectively. Both ofGpTre1andGpTre2were mainly expressed in the head and midgut. The highest expression levels of them were in 5th instar during different development stages. Moreover, knockdown both ofGpTre1andGpTre2by the dsRNAs led to significantly decreased expression of chitin metabolism pathway-related genes, includingGpCHSA,GpCDA1,GpCDA2,GpCHT3a,GpCHT7,GpCHSB,GpCHT-h,GpCHT3b,GpPAGM,andGpUAP, and abnormal phenotypes. Furthermore, the trehalase inhibitor, Validamycin A, treatment increased the expressions ofGpTre1andGpTre2, increased content of trehalose, and decreased the levels of glycogen and glucose. Additionally, the inhibitor caused a significantly increased cumulative mortality ofG. pyloalislarvae on the 2nd (16%) to 6th (41.3%) day, and decreased the rate of cumulative pupation (72.3%) compared with the control group (95.6%). After the activities of trehalase were suppressed, the expressions of 6 integument chitin metabolism-related genes decreased significantly at 24 h and increased at 48 h. The expressions ofGpCHSBandGpCHT-h, involved in chitin metabolism pathway of peritrophic membrane in the midgut, increased at 24 h and 48 h, and there were no changes toGpCHT3bandGpPAGM. These results reveal thatGpTre1andGpTre2play an essential role in the growth ofG. pyloalisby affecting chitin metabolism, and this provides useful information for insect pest control in the future.Keywords:Glyphodes pyloalisWalker;trehalase;chitin metabolism;Validamycin A;RNA interference;pest control
研究领域:
昆虫学
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分子生物学
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害虫防治
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DOI
PubMed
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2023
·
IF 4.7
Yan Song,Fengming Gu,Yijiangcheng Li,Weihong Zhou,Fu-an Wu,Jun Wang,Sheng Sheng
The general cutworm, Spodoptera litura (Lepidoptera: Noctuidae) is a worldwide destructive omnivorous pest and the endoparasitoid wasp Meteorus pulchricornis (Hymenoptera: Braconidae) is the dominant endoparasitoid of S. litura larvae. Trehalase is a key enzyme in insect trehalose metabolism and plays an important role in the growth and development of insects. However, the specific function of trehalase in parasitoid and host associations has been less reported. In this study, we obtained two trehalase genes ( SlTre1 and SlTre2 ) from our previously constructed S. litura transcriptome database; they were highly expressed in 3rd instar larvae. SlTre1 was mainly expressed in the midgut, and SlTre2 was expressed highest in the head. SlTre1 and SlTre2 were highly expressed 5 days after parasitization by M. pulchricornis . Treatment with the trehalase inhibitor validamycin A significantly inhibited the expression levels of SlTre1 and SlTre2 , and the trehalase activity. Besides, the content of trehalose was increased but the content of glucose was decreased 24 h after validamycin A treatment in parasitized S. litura larvae. In addition, the immune-related genes in phenoloxidase (PO) pathway and fatty acid synthesis-related genes in lipid metabolism were upregulated in parasitized host larvae after validamycin A treatment. Importantly, the emergence rate, proportion of normal adults, and body size of parasitoid offspring was decreased in parasitized S. litura larvae after validamycin A treatment, indicating that validamycin A disrupts the trehalose metabolism of parasitized host and thus reduces the fitness of parasitoid offspring. The present study provides a novel perspective for coordinating the application of biocontrol and antibiotics in agroecosystem.
研究领域:
昆虫学
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生物化学
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分子生物学
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寄生虫学
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农业生态学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）