MOLECULES
·
2024
·Vol. 29
·期 13
·3133
·
IF 4.2
Qimei Wu,Jinfen Zheng,Yan Yu,Zhirong Li,Ying Li,Chengfeng Hu,Yaping Zhou,Rongxiang Chen
Vitex negundohas strong antioxidant activity, but its primary antioxidant components are not clear. In this study, the antioxidant components were screened by offline two-dimensional liquid chromatography coupled with electrochemical detection (2D-LC-ECD) and subsequently assessed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) identification, radical scavenging capacity, and molecular docking. Various fractions were isolated fromVitex negundoleaves, and 39 antioxidant components were screened and identified. All of the fractions containing the antioxidant components exhibited certain antioxidant activity. Correlation analysis revealed a strong correlation between the response of LC-ECD and the in vitro antioxidant activity of the fractions. Molecular docking demonstrated that components with high response to LC-ECD exhibited robust interaction with antioxidant-related target proteins. The main antioxidant components ofVitex negundoleaves were isoorientin, chlorogenic acid, agnuside, cynaroside, and scutellarin. The 2D-LC-ECD combined with LC-MS/MS was rapid and effective in screening the antioxidant components inVitex negundoleaves and could also provide technical support for the discovery of antioxidant components with different polarities and contents in other medicinal and edible plants.
研究领域:
植物化学
·
分析化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL SYSTEMATICS AND ECOLOGY
·
2023
·
IF 1.6
Muhammad Aurang Zeb,Peng-Yun Yang,De-Wen Bi,Wen-Chao Tu,Xiao-Li Li,Rui-Han Zhang,Xing-Jie Zhang,Wei-Lie Xiao
Vitex negundo var. Cannabifolia (Sieb. et Zucc.) Hand.-Mazz. leaves and twigs were used to isolate a new phenyldihydronaphthalene-type lignan (1) along with twenty known compounds. Comprehensive spectroscopic techniques such as UV, IR, NMR, HR-ESI-MS, ORD and ECD were used to identify their structures. Quantum chemical computations were used to assign the absolute configurations (AC) of (1) . Using DP4+ analysis, the computed chemical shifts were used to pinpoint relative configuration. Compounds (1–6) were tested for biological activities. Compound (5) significantly decreased the release of lactate dehydrogenase (LDH) by 38% at a concentration of 10 μM, demonstrating that compound (5) had significant anti-inflammatory effects on the activation of the NLRP3 inflammasome , while no compound was found potent at a concentration of 200 μM against tyrosinase . Additionally, the chemotaxonomic significance of the isolated compounds was studied.
研究领域:
植物化学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
PHYTOTHERAPY RESEARCH
·
2023
·Vol. 37
·期 4
·1422-1434
·
IF 7.2
Yanfei Ban,Yang Wang,Liming Qiao,Chengzhong Zhang,Hongrui Wang,Xuhui He,Dan Jia,Chengjian Zheng
The seeds of Vitex negundo have been used for inflammation-related disease treatment in traditional medicine. This study focused on the anti-osteoarthritis (OA) effects of the total lignans of V. negundo seeds (TOV) in monosodium iodoacetate-induced osteoarthritis rats and its pharmacokinetic properties, as well as the effects and potential mechanism of its main components VN1 (6-hydroxy-4-(4-hydroxy-3-methoxy-phenyl)-3-hydro-xymethyl-7-methoxy-3,4-dihydro-2-naphthaldehydeb) and VN2 (vitedoin A) on receptor activator of NF-κB ligand (RANKL)-induced osteoclast differentiation in bone marrow macrophages (BMMs). TOV significantly attenuated osteoarthritis, leading to an increase in pain thresholds, improvement of knee articular cartilages and chondrocytes loss, and decreased total joint scores and serum levels of TNF-α, interleukin-1β (IL-1β), and prostaglandin E2 (PGE2) in osteoarthritis rats. The half-time (T 1/2 ) was 2.82 h and 1.33 h, and the bioavailability was 15.34%–21.89% and 16.29%–22.11%, for VN1 and VN2, respectively. VN2, rather than VN1, remarkably inhibited tartrate-resistant acid phosphatase (TRAP) activity, reduced the number of TRAP-positive multinuclear cells, diminished the formation of actin ring, and decreased mRNA levels of cathepsin K (CTSK), TRAP, nuclear factor of activated T cell 1 (NFATc1), and osteoclast-associated receptor, as well as downregulated protein levels of p-ERK (phosphorylated extracellular signal-regulated kinase), TRAP, CTSK and NFATc1 in BMMs. These findings suggest TOV has promising therapeutic potential for OA treatment and VN2, in particular, attenuates osteoclast differentiation by suppressing ERK/NFATc1 signaling and actin ring, mainly accounting for the anti-OA efficacy of TOV.
研究领域:
药理学
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风湿病学
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传统医学
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分子生物学
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药代动力学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Jiabo Cheng,Mengfei Tian,Zarmina Gul,Chunyu Liang,Bin Qiao,Yuan Gao,Chunjian Zhao,Chunying Li
In this study, a functionalized surface active ionic liquid (SAIL) was designed and prepared, which exhibits pH-responsive behavior and high yield for 6 target flavonoids from the leaves of Vitex negundo L.. Firstly, a series of functionalized SAIL [N-C n , N'–CO 2 H-Im]Br (n = 10, 12, 14, 16) were prepared and used for extraction of the target components, [N-C 14 , N'–CO 2 H-Im]Br showed the highest yield. Secondly, optimized extraction conditions obtained by Plackett-Burman design (PBD) and response surface methodology (RSM) were concentration of 45.5 mM, liquid–solid ratio of 11.6 (mL/g), extraction time of 38.5 min, extraction temperature of 40 °C, and ultrasonic power of 400 W. The yield obtained by [N-C 14 , N'–CO 2 H-Im]Br of vitexin, isovitexin, hyperoside, luteolin, quercetin, apigenin is 2.04, 1.81, 1.24, 1.51, 2.47 and 1.21 mg/g, respectively, which is 1.11–1.56 folds higher than non-functionalized SAIL ([C 14 min]Br) and 1.50–5.36 folds higher than traditional solvents. More importantly, the solvent system can realize the in-situ separation of the target compound by adjusting pH. When pH is changed from 6.1 to 4.9, the system is divided into two phases, and the target compounds were separated to the upper phase with a high recovery rate. Finally, molecular simulation was used to reveal the extraction mechanism of functionalized SAIL on target compounds. The results showed that the target flavonoids were compatibilized to the palisade region of micelles, and the weak interaction between the target flavonoids and functionalized SAIL was mainly van der Waals force.
研究领域:
分析化学
·
药学
·
植物科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）