Plants-Basel
·
2024
·Vol. 13
·期 18
·2576
·
IF 4
Yiqun Hu,Qingqing Chen,Aifang Zhang,Liyuan Zhang,Hansong Dong
Small non-coding RNAs (sRNAs) are pivotal post-transcriptional regulatory factors influencing biological activity. Studies on the rice bacterial blight pathogenXanthomonas oryzaepathovaroryzaestrain PXO99A, previously identified a virulence-associated sRNA, trans3287. A mutant strain lacking this sRNA, named SK01, resulted in markedly diminished virulence towards rice. This study aims to further elucidate the underlying bacterial virulent function of trans3287. The expression of trans3287 was quantified in virulence-inducing and standard nutritional conditions to clarify its production mechanism. The detection of virulence-associated genes revealed that trans3287 regulated the synthesis processes of extracellular polysaccharides, lipopolysaccharides, and the type III secretion system. Moreover, bioinformatics prediction and quantitative PCR indicated a potential direct target of trans3287,PXO_03470, encoding cardiolipin synthase. A dual-plasmid system fusing with GFP tag and protein immunoblotting confirmed that sRNA trans3287 negatively regulatedPXO_03470. Bacterial biofilms demonstrated trans3287 regulated the disruption of biofilm integrity through cardiolipin synthase. This study provides preliminary insights into the mechanistic underpinnings of the role of sRNA trans3287 in mediating bacterial virulence through cardiolipin synthase.
研究领域:
微生物学
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植物病理学
·
分子生物学
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 22
·13692-13699
·
IF 3.269
You Liang,Desong Yang,Jianghu Cui
Bacterial leaf blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most destructive diseases in the growth and development of rice (Oryza sativa L.). Various chemical biocides have been used to manage this disease, which has led to residual contamination in rice, resistance among the Xoo and significant risks to human health. Here, a graphene oxide/silver nanoparticle (GO–Ag) composite was developed and its antibacterial properties against Xoo were also investigated. The results indicated that the GO–Ag composite displayed almost a four-fold enhancement as compared to that of pure Ag NPs. The bacteria were completely inactivated at a relatively low concentration of 2.5 μg mL−1. At the cellular level, the integrity of the cell was damaged and the intercellular contents leaked out by slow-releasing of silver ions from the GO–Ag composite. At the molecular level, this composite induced the production of reactive oxygen species and inhibited DNA replication. More importantly, the phytotoxicity test demonstrated that the as-prepared GO–Ag composite had lower toxicity for the plants than Ag NPs, making it a potential candidate for application in crop disease prevention.
研究领域:
农业科学
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植物病理学
·
纳米生物学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Kunpeng Teng,Qian Liu,Meng Zhang,Hira Naz,Pengcheng Zheng,Xingxing Wu,Yonggui Robin Chi
A new class of chiral pyranone fused indole derivatives were prepared by means of N-heterocyclic carbene (NHC) organocatalysis and demonstrated notable antibacterial activity against Xanthomonas oryzae pv oryzae (Xoo). Bioassays showed that compounds (3S,4R)-5b, (3S,4R)-5d, and (3S,4R)-5l exhibited promising in vitro efficacy against Xoo, with EC50 values of 9.05, 9.71, and 5.84 mg/L, respectively, which were superior to that of the positive controls with commercial antibacterial agents, bismerthiazol (BT, EC50 = 27.8 mg/L) and thiodiazole copper (TC, EC50 = 70.1 mg/L). Furthermore, single enantiomer (3S,4R)-5l was identified as an optimal structure displaying 55.3% and 52.0% curative and protective activities against Xoo in vivo tests at a concentration of 200 mg/L, which slightly surpassed the positive control with TC (curative and protective activities of 47.2% and 48.8%, respectively). Mechanistic studies through molecular docking analysis revealed preliminary insights into the distinct anti-Xoo activity of the two single enantiomers (3S,4R)-5l and (3R,4S)-5l, wherein the (3S,4R)-configured stereoisomer could form a more stable interaction with XooDHPS (dihydropteroate synthase). These findings underscore the significant anti-Xoo potential of these chiral pyranone fused indole derivatives, and shall inspire further exploration as promising lead structures for a novel class of bactericides to combat bacterial infections and other plant diseases.
研究领域:
有机化学
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植物病理学
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药物化学
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DOI
PubMed
高影响因子
PLANT DISEASE
·
2020
·Vol. 104
·期 6
·
IF 3.809
Xian Chen,Pedro Laborda,Fengquan Liu
Rice bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae , is one of the most serious diseases of rice. In this study we found that exogenous melatonin can increase rice resistance to BB. Treatment of rice plants with exogenous melatonin (20 µg/ml) increased nitrate reductase, nitric oxide synthase, and peroxidase activity, enabling high intracellular concentrations of melatonin, nitric oxide, and H 2 O 2 . The expression of NPR1 , a key regulator in the salicylic acid signaling pathway, was upregulated more than 10-fold when the plants were challenged with melatonin. Similarly, the messenger RNA level of PDF1.2 , a jasmonic acid–induced defense marker, was 15 times higher in the treated plants than in the control plants. Moreover, three pathogenesis-related proteins, PR1b , PR8a , and PR9 , were upregulated 20-fold in the presence of melatonin. The application of melatonin (100 µg/ml) to soil-grown rice reduced the incidence of BB by 86.21%. Taken together, these results not only provide a better understanding of melatonin-mediated innate immunity to X. oryzae pv. oryzae in rice but also represent a promising cultivation strategy to protect rice against X. oryzae pv. oryzae infection.
研究领域:
植物病理学
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农学
·
分子生物学
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PubMed
高影响因子
BIOORGANIC CHEMISTRY
·
2025
·
IF 4.7
Yike Yang,Shanshan Su,Zhaoju Sun,Zhouqing Long,Xichun Fu,Jiao Meng,Xiang Zhou,Liwei Liu,Song Yang
The widespread prevalence of bacterial plant diseases imposes a severe constraint on global food production and crop security. To address the growing challenge of bacterial resistance, there is an urgent demand to develop novel agrochemicals that combine high efficacy with low toxicity. In this study, a natural product modification strategy was employed to design new bactericidal candidates with an innovative cation mechanism. Tryptamine was employed as a precursor to synthesize 1,2,3,4-tetrahydro-β-carboline (THC) intermediates via the Pictet-Spengler reaction. Subsequent acylation enabled the introduction of 3- N -substituted propionyl group as flexible bridge chain through an aza-Michael reaction. The resulting racemic THC derivatives were then evaluated for their antimicrobial activity. Notably, molecule B3 demonstrated exceptional inhibitory effects against Xanthomonas oryzae pv. oryzae ( Xoo , EC 50 = 1.32 μg/mL) and Xanthomonas axonopodis pv. citri ( Xac , EC 50 = 2.80 μg/mL), significantly outperforming commercial agents such as bismerthiazol (BT; EC 50 = 40.3 μg/mL for Xoo and 89.6 μg/mL for Xac ) and thiodiazole copper (TC; EC 50 = 58.2 μg/mL for Xoo and 37.3 μ g/mL for Xac ). Moreover, molecule B3 exhibited considerably higher activity than its parent molecule B (EC 50 = 7.27 μg/mL for Xoo and 4.89 μg/mL for Xac ). In vivo assays at 200 μg/mL, B3 provided protective effects of 53.87 % against Xoo and 91.2 % against Xac , exceeding those of TC. Mechanistic investigations revealed that molecule B3 disrupted the intracellular redox balance, and result in the accumulation of reactive oxygen species (ROS) and subsequent induction of apoptosis. These findings not only identify B3 as a promising ROS inducer for bactericide development but also offer novel insights into the role of ROS in combating bacterial diseases.
研究领域:
植物病理学
·
药理学
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DOI
PubMed
高影响因子
MOLECULAR PLANT PATHOLOGY
·
2016
·Vol. 19
·期 1
·49-58
·
IF 4.335
Shanshan Xie,Haoyu Zang,Huijun Wu,Faheem Uddin Rajer,Xuewen Gao
Summary Recent investigations have demonstrated that bacteria employ the volatile compounds they produce during interactions with other organisms, such as plants, fungi, nematodes and bacteria. However, studies focused on the antibacterial activity of plant growth-promoting rhizobacteria (PGPR) volatiles against bacterial phytopathogens are still rare. In this study, Bacillus strain D13, which is antagonistic to Xanthomonas oryzae pv. oryzae ( Xoo ), was isolated and screened. Volatile compounds emitted from strain D13 reduced the colony diameter and cell motility of Xoo cultured in divided Petri plates. Transmission electron micrograph analysis showed concentration in cytoplasm and altered surface morphology in the majority of Xanthomonas cells after co-cultivation with strain D13. Transcriptional expression of virulence-associated genes in Xoo was repressed. Based on gas chromatography/mass spectrometry (GC/MS) analysis, 12 volatile compounds specifically produced by strain D13 were identified. Among them, decyl alcohol and 3,5,5-trimethylhexanol inhibited the growth of Xoo at minimum inhibitory amounts of 0.48 and 2.4 mg, respectively. Furthermore, transcriptional expression of virulence-associated genes was also repressed by decyl alcohol and 3,5,5-trimethylhexanol. These results are useful for a better understanding of the biocontrol mechanisms of Bacillus .
研究领域:
微生物学
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植物病理学
·
农学
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PubMed
高影响因子
PHYTOPATHOLOGY
·
2020
·Vol. 110
·期 5
·
IF 3.234
Ping Yang,Fang-Jing Li,Shi-Wen Huang,Man Luo,Wei Lin,Gao-Qing Yuan,Qi-Qin Li
Berberine, a botanical drug, has great ability to inhibit the growth of Xanthomonas oryzae pv. oryzae . However, the antibacterial mechanism of berberine against X. oryzae pv. oryzae remains poorly understood. In this study, we investigated the physiological and transcriptional response of X. oryzae pv. oryzae to berberine. When strain X. oryzae pv. oryzae GX13 was treated with berberine (10 µg/ml), the hypersensitive response in tobacco, virulence to rice, pathogen population in the rice xylem, production of extracellular polysaccharide (EPS), and activity of extracellular hydrolases decreased, but the levels of pyruvate and ATP increased. Moreover, biofilm formation was inhibited, and the cell membrane was damaged. Transcriptome sequencing analysis showed downregulated expression of gspD , gspE , and gspF , involved in the type II secretion system (T2SS); hrcC , hrcJ , hrcN , and others, involved in the type III secretion system (T3SS); gumB and gumC , associated with EPS; zapE , ftsQ , and zapA , associated with cell division; lpxH , lpxK , kdtA , and others, associated with the membrane; and pyk , pgk , and mdh , encoding pyruvate kinase, phosphoglycerate kinase, and malate dehydrogenase, respectively. Upregulated expression was observed for nuoA , nuoB , and nuoH , encoding the NADH dehydrogenase complex, and atpF , atpC , and atpB , encoding ATP synthase. An adenylate cyclase ( CyaA ) fusion assay showed that berberine affects type three effector protein secretion via the T3SS and reduces effector translocation in X. oryzae pv. oryzae . It is speculated that the negative growth and virulence phenotypes of berberine-treated X. oryzae pv. oryzae GX13 may involve differentially expressed genes associated with cytoarchitecture and energy metabolism, and these effects on primary cell function may further dampen virulence and result in differential expression of T3SS- and T2SS-related genes.
研究领域:
植物病理学
·
微生物学
·
分子生物学
·
药理学
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DOI
PubMed
高影响因子
Biomolecules
·
2026
·Vol. 16
·期 2
·298
·
IF 5.6
Hualing Li,Qi Hu,Nuo Xu,Xueting Shao,Yuxin Liu,Yuxin Hou,Binjie Wang,Jiye Wang,Jianzhuang Yao,Shurong Hou,Xiabin Chen
Heroin is a highly addictive drug that exerts its primary effects through activation of μ-opioid receptors. Its principal active metabolite, 6-monoacetylmorphine (6-MAM), significantly contributes to heroin’s neurological effects and acute toxicity. Current pharmacotherapies for heroin use disorder, employing opioid receptor agonist or antagonist, are often limited by risks of dependence, tolerance, and/or adverse side effects. In this context, enzyme-based therapy emerges as a promising alternative by rapidly converting drugs into inactive or less harmful metabolites in the blood. As a macromolecule, the enzyme does not cross the blood–brain barrier, thereby avoiding side effects in CNS. Through structure-based computational screening, Xoo-PepA (PDB ID: 3JRU), a leucine aminopeptidase from Xanthomonas oryzae pv. oryzae , was identified as a potential enzyme capable of hydrolyzing heroin and 6-MAM. Computational and experimental analyses confirm that Xoo-PepA hydrolyzes heroin sequentially to 6-MAM and subsequently to morphine. Enzymatic properties including dependence on metal ions, optimal pH, thermal stability, and substrate specificity were characterized accordingly. Notably, supplementation with Ni 2+ or Zn 2+ and TCEP extended Xoo-PepA’s half-life at 37 °C from 1 h to over 24 h, highlighting the essential role of metal ions in maintaining structural stability. Moreover, Ni 2+ enhanced Xoo-PepA’s hydrolysis toward peptidase substrate L-leucine-p-nitroaniline by 770-fold, yet conferred no significant activation toward heroin. Mutations in metal ion-coordination residues (e.g., K262A, D267A/E346L) exhibited different activity profiles toward these two types of substrates, suggesting a distinct regulatory mechanism of metal ions may be involved in these activities. This study provides the first demonstration that Xoo-PepA, a non-mammalian, metal-dependent aminopeptidase, can hydrolyze heroin and 6-MAM, shedding light on its functional versatility and biochemical characteristics.
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PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 15
·6190-6197
·
IF 1.821
Xiaoling Wu,Dezhao Kong,Shanshan Song,Liqiang Liu,Hua Kuang,Chuanlai Xu
A pair of sensitive monoclonal antibodies against both Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola were generated by immunizing mice and cell fusion techniques. The sandwich enzyme-linked immunosorbent assay method and immunochromatographic test strip were developed based on newly generated monoclonal antibodies for detection in rice samples. The lower limit of detection of the sandwich ELISA method for X. oryzae pv. oryzae was 1.0 × 104 cfu mL−1. The cut-off values for the immunochromatographic test strip for X. oryzae pv. oryzae and X. oryzae pv. oryzicola in rice samples were 3.3 × 105 cfu mL−1 and 1 × 106 cfu mL−1, respectively. The quantitative sandwich ELISA method and rapid immunochromatographic test strip assay could be useful for food safety inspections.
研究领域:
植物病理学
·
微生物学
·
食品安全
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DOI
高影响因子
PHYSIOLOGIA PLANTARUM
·
2023
·Vol. 175
·期 6
·e14087
·
IF 6.4
Qurban Ali,Abdur Rashid Khan,Sheng Tao,Faheem Uddin Rajer,Muhammad Ayaz,Manzoor Ali Abro,Qin Gu,Huijun Wu,Vladislav Kuptsov,Emilia Kolomiets,Xuewen Gao
Rhizoctonia solani and Xanthomonas oryzae pv. oryzae ( Xoo ) are the two major diseases affecting the quality and quantity of rice production. In the current study, volatile organic compounds (VOCs) of Bacillus spp. were used as green biocontrol agents for plant diseases. In in vitro experiments, Bacillus spp. FZB42, NMTD17, and LLTC93-VOCs displayed strong antimicrobial volatile activity with inhibition rates of 76, 66, and 78% for R. solani and 78, 81, and 76% for Xoo , respectively, compared to control. The synthetic VOCs, namely Pentadecane (PDC), Benzaldehyde (BDH), 1,2-Benz isothiazol-3(2H)-one (1,2-BIT), and mixture (MIX) of VOCs showed high volatile activity with inhibition rates of 86, 86, 89, and 92% against R. solani and 81, 81, 82, and 86%, respectively, against Xoo as compared to control. In addition, the scanning and transmission electron microscopes (SEM and TEM) analyses were performed to examine the effect of Bacillus and synthetic VOC treatments on R. solani and Xoo morphology. The analysis revealed the deformed and irregularized morphology of R. solani mycelia and Xoo cells after VOC treatments. The microscopic analysis showed that the rapid inhibition was due to severe oxidative productions inside the R. solani mycelia and Xoo cells. By using molecular docking, it was determined that the synthetic VOCs entered the active binding site of trehalase and NADH dehydrogenase proteins, causing R. solani and Xoo cells to die prematurely and an accumulation of ROS. In the greenhouse experiment, FZB42, NMTD17, and LLTC93-VOCs significantly reduced the lesions of R. solani 8, 7, and 6 cm, and Xoo 7, 6, and 6 cm, respectively, then control. The synthetic VOCs demonstrated that the PDC, BDH, 1,2-BIT, and MIX-VOCs significantly reduced R. solani lesions on leaves 6, 6, 6, and 5 cm and Xoo 6, 5, 5, and 4 cm, respectively, as compared to control. Furthermore, plant defence-related genes and antioxidant enzymes were upregulated in rice plants. These findings provide novel mechanisms by which Bacillus antimicrobial VOCs control plant diseases.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Cui Zhang,Zhaoli Liu,Xuan Ye,Weijia Mao,Jie Dong,Bin Li,Xueping Zhou,Jinyan Luo,Jianxiang Wu
Enhancing catalytic activity of peroxidase-like nanozymes is essential for developing ultrasensitive lateral flow immunoassay (LFIA). The peroxidase-like catalytic process involves spin-dependent electron transfer during radical formation, and boosting single electron transfer (SET) efficiency is therefore critical to enhance catalytic performance. Here, we fabricated a strained-Co 3 O 4 exhibiting significant atomic compressive strain (ε xx = −3.34 %, ε yy = −6.52 %). The atomic-level displacement direction and distance information determined by atomic displacement analysis (ADA) offer detailed information for density functional theory computational modeling. We discovered that the strain engineering elevated crystal field splitting energy, which promotes the transition of Co 3 O 4 from low-spin states ( t 2g 6 e g 0 ) to high-spin states ( t 2g 4 e g 2 ), resulting in efficient SET performance. Spin-state modulation dramatically enhances the peroxidase-like catalytic efficiency, resulting in 28.0-fold and 11.8-fold lower Michaelis constants ( K m ) for TMB and H 2 O 2 , respectively, than those of the pristine-Co 3 O 4 . In addition, the strained-Co 3 O 4 was successfully employed in the development of LFIA for Xanthomonas oryzae pv. oryzae ( Xoo ) monitoring, and the LFIA achieved a detection sensitivity of 5 × 10 2 CFU mL −1 that was 20-fold higher than Au nanoparticles-based LFIA and was 2–3 orders of magnitude higher than commercial LFIAs. Our findings established a correlation between strain–spin orbit–peroxidase-like activity, offering a promising strategy for the rational design of nanozymes.
研究领域:
纳米酶学
·
免疫学
·
生物传感
·
分子催化
·
植物病理学
·
转化医学
·
材料生物学
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产品关联
DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2015
·Vol. 72
·期 5
·997-1003
·
IF 2.694
Xiaoyu Liang,Yabing Duan,Xiaoyue Yu,Jianxin Wang,Mingguo Zhou
BACKGROUND Bismerthiazol is a commonly used bactericide against rice bacterial leaf blight in China. Although previous research determined that bismerthiazol is susceptible to photolytic degradation, the photodegradation pathway and degradation products, except for 2-amino-5-mercapto-1,3,4-thiadiazole, have remained unknown. RESULTS The photodegradation of bismerthiazol was investigated after 4 and 8 h of irradiation in a solar simulator. Inhibition of Xanthomonas oryzae pv. oryzae ( Xoo ) was greater with a photolysed solution than with a non-photolysed solution of bismerthiazol. Six photoproducts of bismerthiazol were characterised by liquid chromatography coupled with mass spectrometry, and, based on these products, a photodegradation pathway was inferred. Inhibition of Xoo was significantly greater with bismerthiazol and 2-amino-5-mercapto-1,3,4-thiadiazole than with 5-amino-1,3,4-thiadiazole. In addition, Xoo strain 2-1-1 was bismerthiazol- and 2-amino-5-mercapto-1,3,4-thiadiazole resistant in vivo . CONCLUSION Photodegradation increased the inhibitory activity of bismerthiazol against Xoo . The photodegradation pathway was inferred on the basis of the photoproducts of bismerthiazol. In vitro assays indicated that the sulfhydryl group was crucial for the inhibition of Xoo by bismerthiazol and its photoproducts. Bismerthiazol and 2-amino-5-mercapto-1,3,4-thiadiazole might have a similar mode action in vivo and in vitro . © 2015 Society of Chemical Industry
研究领域:
植物病理学
·
农药化学
·
环境科学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）