Mussels are nutrient-rich but perishable, resulting in substantial resource loss. A protease-producing strain (Bacillus velezensisZ-1,Mytilus edulis) isolated from marine sludge was used to hydrolyze mussels, producing Y-1, a hydrolysate with antioxidant activity. In this study, ultrafiltration, gel chromatography, and LC-MS/MS were employed to isolate and identify bioactive peptides from the hydrolysate. The results revealed that the hydrolysate exhibited antioxidant activity, pancreatic cholesterol esterase inhibitory activity, pancreatic lipase inhibitory activity, and α-glucosidase inhibitory activity. Molecular docking using AutoDock Tools 1.5.6 was performed to analyze the interactions of peptides with CD38 and Keap1, leading to the identification of five potentially bioactive peptides: VPPFY, IMLFP, LPFLF, FLPF, and FPRIM. These peptides formed hydrogen bonds and hydrophobic interactions with CD38 and Keap1, demonstrating strong DPPH radical scavenging and superoxide anion radical scavenging capacities. This study highlights the multifunctional bioactive potential of these peptides, offering insights into their therapeutic applications. The findings provide a novel approach for the effective utilization of mussel resources and highlight their potential application value in the development of functional foods.
( Z )-1,3-pentadiene (Zp) exhibits a rigid planar molecular structure and is known for the typical 1,5-sigmatropic hydrogen shift, while it is commonly considered an inert monomer during polymerization. Herein, by utilizing the steric effect of the terminal methyl in Zp, the high trans -1,4-polypentadiene (HTPD) with highly ordered microstructure and well-defined sequence are successfully synthesized using barium dithymol/ tert- butyllithium/ tri -ethylaluminum trimetallic initiators based on anionic polymerization. The significant dependence of polymerization rate and trans -1,4-selectivity on ratio of Ba/Al/Li is demonstrated. The modification in microstructure ( trans -1,4 content ranging from 75 % to 92 %) and stereo-regularity (isotactic triad mm % > 65 %) are confirmed by nuclear magnetic resonance (NMR). Compared with the poly(( Z )-1,3-pentadiene) (PZp) prepared by n -BuLi containing σ -ligand modifiers, the strategy using a large steric initiator to achieve regular insertion of Zp into polymer chains is proved to be effective. Kinetics studies demonstrated that Ba–Al–Li system is characterized by retarded anionic polymerization (RAP), yet it shows a quasi-living process and enabling the preparation of stereometric block polymers such as polyisoprene- b -HTPD (PI- b -HTPD). Thermal performance analysis indicates that the obtained highly ordered polymers show superior thermoplastic properties ( T m = 77.4 °C) with crystallinity as an elastomer. Furthermore, reasonable interpretations for the structure formation, sequence, and rate reduction effected by the Ba/Al/Li trimetallic initiator are proposed.
危险品化学品经营许可证（带存储）