BMC MICROBIOLOGY
·
2024
·Vol. 24
·期 1
·1-12
·
IF 4
Sun Zongping,Fang Yuting,Zhu Yaohuan,Tian Wen,Yu Junjie,Tang Jun
Background The mycotoxin zearalenone (ZEA) produced by toxigenic fungi is widely present in cereals and its downstream products. The danger of ZEA linked to various human health issues has attracted increasing attention. Thus, powerful ZEA-degrading or detoxifying strategies are urgently needed. Biology-based detoxification methods are specific, efficient, and environmentally friendly and do not lead to negative effects during cereal decontamination. Among these, ZEA detoxification using degrading enzymes was documented to be a promising strategy in broad research. Here, two efficient ZEA-degrading lactonases from the genus Gliocladium, ZHDR52 and ZHDP83, were identified for the first time. This work studied the degradation capacity and properties of ZEA using purified recombinant ZHDR52 and ZHDP83.Results According to the ZEA degradation study, transformed Escherichia coli BL21(DE3) PLySs cells harboring the zhdr52 or zhdp83 gene could transform 20 µg/mL ZEA within 2 h and degrade > 90% of ZEA toxic derivatives, α/β-zearalanol and α/β-zearalenol, within 6 h. Biochemical analysis demonstrated that the optimal pH was 9.0 for ZHDR52 and ZHDP83, and the optimum temperature was 45 °C. The purified recombinant ZHDR52 and ZHDP83 retained > 90% activity over a wide range of pH values and temperatures (pH 7.0–10.0 and 35–50 °C). In addition, the specific activities of purified ZHDR52 and ZHDP83 against ZEA were 196.11 and 229.64 U/mg, respectively. The results of these two novel lactonases suggested that, compared with ZHD101, these two novel lactonases transformed ZEA into different products. The slight position variations in E126 and H242 in ZDHR52/ZEA and ZHDP83/ZEA obtained via structural modelling may explain the difference in degradation products. Moreover, the MCF-7 cell proliferation assay indicated that the products of ZEA degradation using ZHDR52 and ZHDP83 did not exhibit estrogenic activity.Conclusion sZHDR52 and ZHDP83 are alkali ZEA-degrading enzymes that can efficiently and irreversibly degrade ZEA into non-estrogenic products, indicating that they are potential candidates for commercial application. This study identified two excellent lactonases for industrial ZEA detoxification.
研究领域:
微生物学
·
食品科学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Zhipeng Huang,Juan He,Yuanyuan Li,Chaojun Wu,Liqin You,Hongliang Wei,Kai Li,Shusheng Zhang
Surface molecularly imprinted polymers (MIL-101@MIPs) were prepared using MIL-101 as supporting core, imprinted polymers as selective shell synthesized with coumarin-3-carboxylic acid as dummy template of Zearalenone (ZEN), methacrylic acid as functional monomer, and ethylene glycol dimethacrylate hydroxyethyl methacrylate as cross-linker. The polymers were characterized by scanning electron microscopy, transmission electron microscopy, energy–dispersive X-ray spectroscopy, X-ray diffraction, and particle-size distribution analyses. MIL-101@MIPs were used as the sorbent to compose the self-made cartridge. The cartridge was used to purify and enrich ZEN from real samples. Under optimized SPE conditions, a self-made cartridge can be reused for at least seven cycles. Elution was monitored with a high-performance liquid chromatography–fluorescence detection system. The linearity of the method ranged within 6.25–250 ng kg −1 . The limits of detection ranged within 2.09 – 4.16 ng kg −1 , and the limits of quantification ranged within 6.25 –12.50 ng kg −1 , respectively. The matrix effects of four real samples were discussed. The spiking recoveries of ZEN ranged within 81.70%–90.10% with relative standard deviations lower than 5.56%. The performance of the self-made cartridge and immunoaffinity column was compared by chromatography.
研究领域:
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Toxins
·
2024
·Vol. 16
·期 3
·121
·
IF 3.9
Hira Sayed,Qiongqiong Zhang,Yu Tang,Yanan Wang,Yongpeng Guo,Jianyun Zhang,Cheng Ji,Qiugang Ma,Lihong Zhao
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin found in many agricultural products and can cause reproductive disorders, mainly affecting spermatogenesis in male animals. Rutin (RUT) is a natural flavonoid compound recognized for its significant antioxidant, anti-inflammatory and estrogenic properties. The present study aimed to determine the protective role of RUT against ZEN-induced reproductive toxicity in male mice. Twenty-four adult Kunming male mice were divided into four groups: control, RUT (500 mg/kg RUT), ZEN (10 mg/kg ZEN), ZEN + RUT (500 mg/kg RUT + 10 mg/kg ZEN), with six replicates per treatment. The results indicated that RUT mitigated ZEN-induced disruption in spermatogenic cell arrangement, decreased spermatozoa count, and increased sperm mortality in the testes. RUT significantly restored ZEN-induced reduction in T, FSH, LH, and E2 serum levels. Moreover, RUT mitigated ZEN-induced apoptosis by increasing the mRNA expression level ofbcl-2, decreasing the mRNA expression level ofkiss1-r, and decreasing the protein expression level of caspase 8 in reproductive tissues. These findings indicate the protective role of RUT against ZEN-induced reproductive toxicity in male mice by regulating gonadotropin and testosterone secretions to maintain normal spermatogenesis via the HPG axis, which may provide a new application direction for RUT as a therapeutic agent to mitigate ZEN-induced reproductive toxicity.
研究领域:
毒理学
·
生殖生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2025
·
IF 5.4
Sohail Mumtaz,Muhammad Imran,Amir Muhammad Afzal
In this study, we develop photoresponsive heterogeneous Cobalt-nickel-based MOFs (CoNi-MOFs), cobalt dioxide (CoO 2 ), and tungsten diselenide (WSe 2 ) hybrid device to improve the performance of photo-assisted supercapacitors. We measure the electrochemical characteristics of photo-assisted hybrid electrodes under both dark and light conditions using cyclic voltammetry and galvanostatic charge–discharge. In dark, a high specific capacity (Qs) is estimated at 970 C/g, increased to 1230 C/g under the light in three-electrode assemblies. An FTO/CoNi-MOF@CoO 2 @WSe 2 /Electrolytes//PANI@AC/GS photo hybrid supercapacitors device is designed and measured in both conditions. The hybrid device showed Qs of 325 C/g in light conditions with energy and power densities of 72 Wh/kg and 827 W/kg, respectively. Even after undergoing 6000 cycles, it exhibited a capacity retention (CR) rate of 91 % and columbic efficiency of 93 %, indicating its exceptional durability as an energy storage device. Besides, the composite devices detect zearalenone in agricultural food samples. The optimal signal for Zearalenone in phosphate buffer solution was observed at pH 7.0, yielding the highest detected response. The sensor demonstrated a broad linear dynamic range of 1–300 μM and an impressively low detection limit of 0.5 nM for Zearalenone concentration. The CoNi-MOF@CoO 2 @WSe 2 nanocomposite has a remarkable sensitivity value of 110.72 mA/cm −2 /mM −1 and response against zearalenone in light. The findings also suggest that the capacitance of the CoNi-MOF@CoO 2 @WSe 2 electrode may increase at an optimal temperature. This likely occurs due to enhanced ion diffusion and charge transfer kinetics, which can improve the energy storage device’s efficiency and aid in identifying the cancerous part of the body.
研究领域:
材料科学
·
分析化学
·
能源存储
·
食品安全检测
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Yuting Zhou,Xiang Chen,Xianlu Lei,Meiqi Huang,Miaomiao Ma,Jian Zeng,Fuyan Zhang,Shuang Li,Qi Sun,Tao Le
Zearalenone (ZEN), a prevalent mycotoxin found in cereal crops, poses a significant threat to food safety and human health. To address this issue, there is an urgent need for rapid, sensitive, and cost-effective detection methods. In this study, we developed a novel bimodal aptasensor based on NH 2 -UiO-66@MB composites for ZEN detection. Methylene blue (MB) was encapsulated within NH 2 -UiO-66 nanoparticles to enhance fluorescence intensity, and the ZEN aptamer was attached to NH 2 -UiO-66 via Zr–O–P bonds and Coulombic forces to create a gating effect. In the presence of ZEN, the aptamer bounded to ZEN, triggering a conformational change that released MB, resulting in decreased fluorescence intensity and absorbance. Under optimal conditions, the bimodal aptasensor exhibited wide linear ranges (2.5–300 ng/mL for colorimetry and 0.125–200 ng/mL for fluorescence) and low limits of detection (1.16 ng/mL for colorimetry and 0.03 ng/mL for fluorescence). Furthermore, the aptasensor demonstrated high specificity and reproducibility, with recovery rates ranging from 97.43 % to 103.71 % in spiked cereal products. These findings highlight the potential of the developed bimodal aptasensor as a novel tool for the rapid, accurate, and cost-effective detection of ZEN in cereal products, contributing to improved food safety monitoring.
研究领域:
食品安全
·
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Lanxue Li,Yingguo Bai,Yifan Zhang,Xing Qin,Xiaolu Wang,Jie Zhang,Yaru Wang,Huiying Luo,Bin Yao,Tao Tu
Zearalenone (ZEN), a prevalent and stable Fusarium mycotoxin in cereals, threatens food safety and resists conventional processing. Natural ZEN hydrolases are often inadequate under industrial thermal and acidic conditions. Here, we engineered a robust variant, R76Q/T217C-G242C, via a hybrid computational strategy integrating energy calculations, electrostatic optimization, consensus analysis, and disulfide bond design. The resulting biocatalyst completely degraded 16.6 mg/L ZEN within 30 min, retained 71 % activity after 5 min at 55 °C (10-fold improvement), and exhibited >75 % activity at pH 5.0 with a 192.5-min half-life at 40 °C. It also eliminated 95.13 % of naturally occurring ZEN in moldy distillers' grains. Structural and dynamic analyses revealed stabilization mechanisms mediated by a designed disulfide bond and optimized interaction networks. This study provides a high-performance enzymatic solution for ZEN degradation in challenging food-processing environments.
研究领域:
食品科学与技术
·
酶工程
·
霉菌毒素学
·
计算生物学
·
生物催化
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2026
·
IF 3.2
Ruo Du,Qingyuan Li,Yi Cheng,Yuting Zeng,Mairewulan Abula,Wenyi Liu,Fangfang Yu,Weihua Jia,Yuhui Du,Yue Ba,Yanjie Wang,Guoyu Zhou
Zearalenone (ZEA), a mycotoxin produced by Fusarium fungi, has drawn significant attention due to its multi-organ toxicity. To explore the molecular mechanisms of ZEA-induced hepatotoxicity and identify effective intervention strategies, this study identified key targets associated with ZEA-induced hepatotoxicity by screening public databases. Further construction of a protein interaction network revealed five crucial targets: TP53 , STAT3 , HSP90AA1 , ESR1 , and PPARG . Moreover, genistein (GEN) was identified from the TCMSP database as a compound capable of simultaneously targeting these targets. Molecular docking results indicated that GEN exhibits strong binding affinity with the five targets (all binding energies <0 kcal/mol). To validate hepatotoxicity mediated by ZEA and intervention mechanisms of GEN, ZEA exposure and ZEA + GEN treated mouse models were conducted. The results exhibited that exposure to ZEA led to increased liver weight and liver coefficient as well as abnormal changes in typical hepatotoxicity indicators in mice, while GEN can alleviate the aforementioned changes. Furthermore, results from quantitative RT-PCR and Western blot indicated that hepatic Hsp90aa1 and HSP90α (the protein encoded by the Hsp90aa1 gene) expression was significantly upregulated by ZEA but significantly downregulated after GEN supplementation. In conclusion, HSP90α may be a key regulatory node in GEN treatment of hepatotoxicity caused by ZEA. Download: Download high-res image (558KB) Download: Download full-size image
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2026
·
IF 6.7
Dan Wu,Jiawei Liu,Feng Zhang,Muhammad Irfan,Zhibin Xu,Lili Qiu,Junming Geng,Yifei Wang,Zihui Meng
Zearalenone (ZEN) and its metabolites are major mycotoxins commonly found in cereals and cereal-based products, posing significant risks to food safety and human health. Therefore, developing effective methods to detect ZEN and its primary metabolites is crucial. In this study, a label-free optical sensing platform based on the photonic crystal immuno-hydrogel (PCIH) was presented. We developed a competitive immunoassay for the broad-spectrum detection of zearalenone-type mycotoxins (ZEN, α-zearalenol (α-ZEL), and β-zearalenol (β-ZEL)), using the antibody 3D4, which specifically targets ZEN and its metabolites, as the recognition element, and photonic crystal hydrogel (PCH) as the carrier. The PCIH sensor demonstrated good linearity for ZEN, α-ZEL, and β-ZEL across a concentration range of 1-150 ng/mL, with limits of detection (LODs) of 1.97, 2.06, and 2.07 ng/mL, respectively, while exhibiting excellent selectivity against interfering substances. The sensor also exhibited good reusability after five regeneration cycles and maintained stable performance after 21 days of storage. Furthermore, the sensor was successfully applied to detect zearalenone-type mycotoxins in corn grits, wheat flour, and rice samples, showing strong reliability and practicality. This PCIH sensing platform is simple to operate and label-free, offering a promising and efficient approach for monitoring zearalenone-type mycotoxins in food.
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2023
·
IF 5.9
Dixuan Lai,Zhaode Mu,Jianli Zuo,Jiaxu Zhou,Qifan Zhang,Keyu Chen,Lijuan Bai,Hongwen Liang
Synthesis of efficient electrochemiluminescent (ECL) luminescent materials is one of the effective means to improve the sensitivity of sensors. In this study, an efficient ECL-emitting nanomaterial, lanthanide metal–organic framework (PTCA-La-MOF, PLM) decorated with polycyclic aromatic hydrocarbon (PAH) derivative perylene-3,4,9,10-tetracarboxylic acid (PTCA), was synthesized for the sensitive detection of zearalenone (ZEN). The synthesized PLM nanomaterials effectively incorporated nanoscale features, allowing for efficient mass loading of PTCA while addressing the drawbacks of poor stability and easy aggregation in water. Moreover, the PLM exhibited a 2.4-fold increase in the ECL efficiency compared to PTCA aggregates due to the increase in ligand molecular spacing in PLM mitigated the aggregation-caused quenching (ACQ) effect of PTCA. Furthermore, the substrate material polyethylenimine (PEI)-functionalized NG nanocomposite doped with NiZn MOF (NG-PEI-NiZn MOF) could be used as an excellent sensing platform due to the reason that NG-PEI-NiZn MOF exhibited large specific surface area and excellent metallic conductivity. We demonstrated that ECL immunosensors using PLM as the signal amplifier and NG-PEI-NiZn MOF as the sensing matrix showed a significantly enhanced ECL signal with excellent selectivity for ZEN. We envision that the signal amplification strategy not only provided an effective approach for the detection of ZEN in food and cereals but also provided a convenient way to overcome the ACQ effect of PAHs and provide the ECL efficiency.
✓ 已验证
产品关联
DOI
高影响因子
Food Analytical Methods
·
2025
·Vol. 19
·期 1
·35
·
IF 3
Hu Wei,Jiang Donglei,Xiang Xinyue,Chen Chao,Wang Nanwei,Jiang Hui,Zhang Na,Wang Lifeng
This study presents the development of an electrochemical sensor based on nanochannels for the sensitive detection of the fungal toxin zearalenone (ZEN). The sensor incorporates gold nanoparticles (AuNPs) and multi-walled carbon nanotubes (cMWCNTs) onto anodic aluminum oxide (AAO), with nickel oxide (NiO) modification on the reverse side of the AAO, thereby forming a NiO-AAO@AuNPs-cMWCNTs/SPCE electrode system. The NiO modification facilitates the initial oxidation of ZEN, resulting in the generation of distinctive electrochemical oxidation peaks. To investigate the reaction mechanism of ZEN oxidation, electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were employed. In the concentration range of 1 ~ 40 μg/mL, the sensor showed a clear linear correlation between ZEN concentration and the impedance response, expressed by the regression equation: R EIS (Ω) = 40.8 + 2.7 C ZEN (μg/mL) (R 2 = 0.997, n = 3). The limit of detection (LOD) was determined to be 0.1 μg/mL. This nanochannel-based electrochemical platform provides a reliable and efficient strategy for ZEN detection, demonstrating the potential of nanostructured materials in food safety monitoring.
研究领域:
食品安全
·
电分析化学
·
纳米材料
·
环境监测
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Ruxia Liu,Ruirui Shi,Wenting Zou,Wenhua Chen,Xianchao Yin,Fengchun Zhao,Zhengyou Yang
Here we demonstrate a novel phage-magnetic-chemiluminescent enzyme immunoassay (P-MCLEIA) for detection of zearalenone (ZEN). The P-MCLEIA was more efficient than conventional ELISA through several improvements. In the P-MCLEIA, magnetic nanoparticles were replaced of microplates as solid phases to reduce the whole incubation time within 40 min. Phage-mimotope was replaced of chemosynthetic antigen to improve the sensitivity of immunoassay. Chemiluminescence substrate was replaced of chromogenic substrate to further improve the sensitivity. The IC 50 value of P-MCLEIA was 31.4 pg/mL, which was about 11 times lower than that of phage-magnetic-enzyme linked immunosorbent assay (P-MELISA) and 72 times lower than that of conventional ELISA. The LOD of P-MCLEIA was 4.3 pg/mL. Recovery study of P-MCLEIA was performed by analyzing ZEN levels in spiked corn samples, intra- and inter-assay recoveries were 80.0–119.8% and 82.7–112.7%, respectively. Furthermore, parallel analysis of natural corn samples showed a good correlation between the P-MCLEIA and high performance liquid chromatography.
研究领域:
食品安全检测
·
免疫学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Hongxia Tan,Hongyuan Zhou,Ting Guo,Jiaxin Li,Chi Zhang,Shuo Wang,Yuhao Zhang,Liang Ma
Zein, the plant protein, has received great attention in the last years. However, hidden zearalenone (ZEN), the zein-bound ZEN present in zein would have a great hazard to humans and animals. To date, the fates of hidden ZEN under different extraction methods of zein have not been clarified. In this study, the effect of zein extraction methods on conversion of free ZEN with hidden ZEN and its corresponding mechanism were studied. Results showed that the acid extraction condition promoted the formation of hidden ZEN (up to 92.03%), which was closely related to increased hydrophobic cavity of zein. However, alkaline extraction condition caused the conversion of hidden ZEN (from 58.82% to 13.33%) into free ZEN (from 41.18% to 86.67%), which was attributed to the great denaturation of zein. This study is of great significance for controlling hidden ZEN during zein extraction and maize processing.
研究领域:
食品科学
·
毒理学
·
农业化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）