CERAMICS INTERNATIONAL
·
2024
·
IF 5.1
Ting Yao,Xianxiang Zeng,Tao Luo,Xueying Tao,Hengyi Xu
Microbial contamination poses a serious threat to human health and socioeconomics, and is a major problem for global public health. Herein, we fabricated Ti 3 C 2 T x /ZnONPs composite by using Lactobacillus plantarum fermentation broth to reduce ZnONPs in situ on few-layer Ti 3 C 2 T x MXene after etching and delamination treatment. Antibacterial experiments showed that the Ti 3 C 2 T x /ZnONPs composite exhibited significant antibacterial and antibiofilm activity against both Gram-negative E. coli and Gram-positive S. aureus . Moreover, the exploration of the antibacterial mechanisms demonstrated that Ti 3 C 2 T x /ZnONPs could inhibit and kill bacteria by disrupting bacterial cell wall/membrane, causing leakage of intracellular contents, interfering with metabolism and inducing oxidative stress. The synergistic effect of multiple antibacterial mechanisms can effectively prevent the evolution of resistance. Overall, as a novel inorganic antibacterial material, Ti 3 C 2 T x /ZnONPs composite holds a promising application in the antimicrobial field.
研究领域:
材料科学
·
纳米技术
·
微生物学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2019
·Vol. 20
·期 19
·4796
·
IF 4.183
Lisha Ai,Huawei He,Peng Wang,Rui Cai,Gang Tao,Meirong Yang,Liying Liu,Hua Zuo,Ping Zhao,Yejing Wang
The interests of developing antimicrobial biomaterials based on silk sericin fromBombyx moricocoon, have been shooting up in the last decades. Sericin is a valuable natural protein owing to its hydrophilicity, biodegradability, and biocompatibility. Here, we fabricated a sponge with antibacterial capacities for potential wound dressing application. By co-blending of sericin, polyvinyl alcohol (PVA) and zinc oxide nanoparticles (ZnONPs), the ZnONPs-sericin/PVA composite sponge (ZnONPs-SP) was successfully prepared after freeze-drying. Scanning electron microscopy showed the porous structure of ZnONPs-SP. Energy dispersive spectroscopy indicated the existence of Zn in the sponge. X-ray diffractometry revealed the hexagonal wurtzite structure of ZnONPs. Fourier transform infrared spectroscopy showed the biologic coupling of ZnONPs and sericin resulted in a decrease of α-helix and random coil contents, and an increase of β-sheet structure in the sponge. The swelling experiment suggested ZnONPs-SP has high porosity, good hydrophilicity, and water absorption capability. The plate bacterial colony counting coupled with growth curve assays demonstrated that the composite sponge has an efficiently bacteriostatic effect againstStaphylococcus aureusandEscherichia coli, respectively. Furthermore, the cell compatibility analysis suggested the composite sponge has excellent cytocompatibility on NIH3T3 cells. In all, ZnONPs-SP composite sponge has significant potentials in biomaterials such as wound dressing and tissue engineering.Keywords:antimicrobial sponge;rational design;ZnONPs;cytocompatibility
研究领域:
生物材料学
·
医学工程
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2017
·
IF 6.065
Zheng-Zhe Zhang,Jia-Jia Xu,Zhi-Jian Shi,Ya-Fei Cheng,Zheng-Quan Ji,Rui Deng,Ren-Cun Jin
Previous studies investigating the risk of engineered nanoparticles (NPs) to biological wastewater treatment have primarily tested NPs individually; however, limited data are available on the impact of NPs on the anaerobic ammonium oxidation (anammox) process. In this study, the toxicity of CuNPs on anammox granules was investigated individually and in combination with CuONPs or ZnONPs. Exposure to CuNPs at 5 mg g −1 suspended solids (SS) decreased the anammox activity to 47.1 ± 8.5%, increased the lactate dehydrogenase level to 110.5 ± 3.4% and increased the extracellular N 2 H 4 concentration by 16-fold but did not cause oxidative stress. The presence of CuONPs or ZnONPs at 5 mg g −1 SS did not significantly aggravate or alleviate the toxicity of the CuNPs; however, the introduction of EDTA or S 2− could attenuate the adverse effects of the CuNPs, CuONPs and ZnONPs on the anammox granules. EDTA captured Cu ions, whereas S 2− shielded and deactivated Cu ions and passivated CuNPs. Therefore, our results indicated that the toxicity of NPs was dependent on the amount of active metal reaching the anammox cells. Overall, the results of this study have filled knowledge gaps and provided insights into the combined toxicity of NPs on anammox biomass.
研究领域:
环境工程
·
微生物学
·
水处理技术
✓ 已验证
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Ting Yao,Xianxiang Zeng,Yuying Zou,Tao Luo,Xueying Tao,Hengyi Xu
Novel food packaging materials with antimicrobial properties must be developed to ensure that fresh agricultural products are free from microbial contamination during the process from “farm” to “table”. Herein, we synthesized an antimicrobial composite packaging film by employing a green in situ synthesis method. The method involves the in situ reduction of zinc oxide nanoparticles (ZnONPs) on the substrate Ti 3 C 2 T x by Lactobacillus plantarum fermentation broth, which was then blended with polyvinyl alcohol and vanillin to obtain the polyvinyl alcohol/vanillin/Ti 3 C 2 T x /ZnONPs (PVA/VAN/T-Z) composite film. The incorporation of crosslinker VAN and antimicrobial material Ti 3 C 2 T x /ZnONPs did not affect the transparency and color of the film, but improved its mechanical strength, hydrophobicity, and antimicrobial ability of the film. Antimicrobial experiments demonstrated that the PVA/VAN/T-Z composite film exhibited significant antimicrobial activity against bacteria (Gram-negative E. coli and Gram-positive S. aureus ) and fungi isolated from peppers. Furthermore, pepper preservation experiments demonstrated that the PVA/VAN/T-Z composite film exhibited commendable antimicrobial and preservation performance compared to commercially available film. As a novel antimicrobial packaging material, the multifunctional PVA/VAN/T-Z composite film has a broad application prospect in food preservation.
研究领域:
食品科学
·
材料科学
·
微生物学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Mei Zhang,Qianqian Kong,Jingcheng Huang,Yaodong Xiang,Guanjie Wang,Jie Han,Yemin Guo,Shancang Zhao,Xia Sun
Within this work, an electrochemiluminescence (ECL) aptasensor was constructed for the sensitive detection of diazinon . Primarily, AuNPs not only provided a platform for luminol fixation due to their favorable bioactivity and conductivity , but they facilitated the improvement of ECL signals. Furthermore, three-dimensional (3D) flower-like ZnONPs were obtained by hydrothermal synthesis , which could be an efficacious catalyst for the luminol-hydrogen peroxide (luminol-H 2 O 2 ) system, whereby the ECL signal of the sensor could be amplified. Given the large specific surface area of 3D flower-like ZnONPs, modification of positive charges on their surface could well immobilized the electronegative aptamer chains on the electrode surface , and when diazinon (DZN) was added, the aptamer combined with DZN to form a complex to hinder the ECL intensity, and the detection of DZN was achieved by the change of ECL signal intensity. After evaluated the experimental parameters, the detection limit of DZN was 0.0109 nM (S/N = 3), and the linear range was 0.0328–3.28 × 10 4 nM. The proposed ECL aptasensor had comparatively satisfactory detection limits, which provided a reasonable reference for other contaminant residues detection.
研究领域:
分析化学
·
环境科学
·
食品安全
·
纳米技术
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
·
2023
·
IF 6.1
Jiaqi Zhang,Jiaxin Li,Xiao-Fei Zeng,Jie-Xin Wang,Yuan Le
Bacterial infection is a general problem that increases the burden of wound treatment. It has been intensely expected to fabricate bioactive wound dressings with good antibacterial effect. In this work, double-layer hybrid nanofiber membranes (DHNM) of PVA/COL@ZnONPs and PCL/Gt@TiO 2 NPs were built by continuous electrospinning. The matrix membranes with acceptable tensile strength and moisture permeability property were obtained by optimizing the formulation and spinning conditions. The matrix membranes exhibited Young’s modulus of 91.93 MPa and tensile stress of 2.61 MPa. The water vapor transmittance rate (WVTR) of matrix membranes was 1140.45 g/m 2 ·day. ZnONPs in the inner layer were released quickly in 2 hours to destroy the bacteria in the wound. TiO 2 NPs in the outer layer were sustainably released within 7 days to resist the bacterial attack outsides. The double-layer membranes showed good biocompatibility and enhanced the migration of cells. The cell migration rate of double-layer hybrid membranes (DHNM) was higher than that of single layer membranes. The double-layer membrane showed 93% elimination of the wound specific P. aeruginosa , over 99% inhibition to E. coli and over 97% to S. aureus . This study provides a potential approach for wound dressings to improve antibacterial effect.
研究领域:
材料科学
·
纳米技术
·
生物医学工程
·
伤口护理
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）