Small
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2024
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IF 13
Huacheng He,Wen Huang,Shihui Zhang,Jie Li,Jian Zhang,Bingxin Li,Jie Xu,Yuting Luo,Huiling Shi,Yue Li,Jian Xiao,Odinaka Cassandra Ezekiel,Xiaokun Li,Jiang Wu
Severe burn wounds usually destroy key cells’ functions of the skin resulting in delayed re-epithelization and wound regeneration. Promoting key cells’ activities is crucial for burn wound repair. It is well known that keratinocyte growth factor-2 (KGF-2) participates in the proliferation and morphogenesis of epithelial cells while acidic fibroblast growth factor (aFGF) is a key mediator for fibroblast and endothelial cell growth and differentiation. However, thick eschar and the harsh environment of a burn wound often decrease the delivery efficiency of fibroblast growth factor (FGF) to the wound site. Therefore, herein a novel microneedle patch for sequential transdermal delivery of KGF-2 and aFGF is fabricated to enhance burn wound therapy. aFGF is first loaded in the nanoparticle (NP aFGF ) and then encapsulated NP aFGF with KGF-2 in the microneedle patch (KGF-2/NP aFGF @MN). The result shows that KGF-2/NP aFGF @MN can successfully get across the eschar and sequentially release KGF-2 and aFGF. Additional data demonstrated that KGF-2/NP aFGF @MN achieved a quicker wound closure rate with reduced necrotic tissues, faster re-epithelialization, enhanced collagen deposition, and increased neo-vascularization. Further evidence suggests that improved wound healing is regulated by significantly elevated expressions of hypoxia-inducible factor-1 alpha (HIF-1ɑ) and heat shock protein 90 (Hsp90) in burn wounds. All these data proved that KGF-2/NP aFGF @MN is an effective treatment for wound healing of burns.
研究领域:
生物医学工程
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药剂学
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组织修复与再生
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Yuting Luo,Hangqi Luo,Xiaying Yang,Xin Ding,Kun Wang,Miao Zhang,Jiacheng Wei,Ying An,Jie Xu,Huacheng He,Jiang Wu
The application of acidic fibroblast growth factor (aFGF) has shown great potential in the treatment of scald or burn wounds with high morbidity and mortality, especially in promoting the repair of deep partial-thickness wounds. However, its short half-life and instability in vivo do pose challenges for clinical application. Herein, two kinds of bio-inspired modified piezoelectric chitosan (CS) films, namely heparin-coated CS film (HCS) and polydopamine-coated CS film (DCS), are facially fabricated and adopted as controlled-release platforms for local delivery of aFGF. Polydopamine or heparin layers serve as a bridge grafting on chitosan films, facilitating the loading of aFGF and enabling controlled release of aFGF from the piezoelectric film through intermolecular interactions. Additionally, these layers enhance the hydrophilicity and antibacterial properties of the bare CS film due to their inherent biological activities. Furthermore, the polydopamine coating imparts photothermal activity to the CS film. The in vivo experiments ascertained that the synergetic effect of the controlled-released aFGF and low temperature photothermal therapy collectively accelerate scald wound healing outcomes within 14 days by facilitating granulation formation, collagen deposition, re-epithelialization and angiogenesis. This study opens up new possibilities for the development of multifunctional chitosan-based wound dressings and the creation of innovative drug delivery platforms.
研究领域:
生物医学工程
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材料科学
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伤口愈合
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药物递送系统
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组织工程
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Hong Zhu,Hangqi Luo,Minjie Lin,Yuan Li,Anqi Chen,Huacheng He,Feixia Sheng,Jiang Wu
Pressure ulcer (PU) in patients with diabetes mellitus (DM) is still a clinical intractable issue due to the complicated physiological characteristics by the prolonged high glucose level and impaired angiogenesis. The PU treatment includes surgical debridement, stem cell therapy and growth factors, leading to high cost and repeated professional involvement. Developing effective wound dressing combining the therapeutic cells and growth factors has become highly demanded. Herein, we reported the direct subcutaneous administration of endothelial progenitor cells (EPCs) and acid fibroblast growth factor (aFGF) with a shape-memorable methacrylated gelatin cryogel (EPCs/ [email protected] ) for the therapy of PU in rats with DM. This EPCs/ [email protected] cryogel system presented microporous structure, elastic mechanical strength and enhanced cell migration property with controlled release of aFGF. Moreover, compared with EPCs/aFGF and GelMA alone, in vivo results showed that this EPCs/ [email protected] system exhibited accelerated wound closure rate, enhanced granulation formation, collagen deposition as well as re-epithelization. Importantly, we found that the excellent positive performance of EPCs/ [email protected] is due to its up-regulation of HIF-ɑ upon the wound site, modulating the microenvironment of wound site to initiate the impaired local angiogenesis. Collectively, this hybrid gelatin cryogels show great promise for biomedical applications, especially in tissue engineering and regenerative medicine.
研究领域:
生物医学工程
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再生医学
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组织工程
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伤口愈合
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糖尿病
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
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2025
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IF 11.5
Anqi Ye,Hao Mei,Zheng Zhang,Fuqiang Song,Linyuan Jiang,Ting Huang,Ping Li,Siting Du,Yanzhen Feng,Tianyao Jiang,Fenzan Wu,Li Cheng,Jia Qu,Jian Xiao
Severe corneal injuries can cause visual impairment even blindness. Surgically stitching or implanting biomaterials have been developed, but their implementation requires professional surgeons, failing to address the immediate need of medical treatment. The pressing challenge lies in developing multifunctional biomaterials that enable self-management of corneal injuries. This study introduces collagen-based hydrogels that can be used as contact lenses, incorporating macromolecular collagen into common polymer materials via a dual-step orthogonal cross-linking process. This method ensures superior optical and mechanical performance while preserving the bioactivity and structural stability of the incorporated materials. Specialized contact lens facilitates the controlled release of labile protein therapeutics such as acidic fibroblast growth factor (aFGF), eliminating the need for stabilizers like heparin. This capability allows the lens to deliver a wide range of labile proteins, thus expanding its therapeutic use across various ophthalmic and potentially other medical conditions. The lens's anti-inflammatory and anti-fibrotic properties effectively treat corneal alkali burn. Termed ‘corneal first-aids lens’, it can provide postoperative clinical care and serve as a viable and safe therapeutic alternative for patients with limited medical access.
研究领域:
生物医学工程
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眼科
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材料科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Lu Liu,Enling Hu,Haoyu Qiu,Qian Xu,Kun Yu,Ruiqi Xie,Fei Lu,Qi Wang,Bitao Lu,Qing Li,Guangqian Lan
Skin wound healing in dynamic environments remains challenging. Conventional gels are not ideal dressing materials for wound healing due to difficulties in completely sealing wounds and the inability to deliver drugs quickly and precisely to the injury. To tackle these issues, we propose a multifunctional silk gel that rapidly forms strong adhesions to tissue, has excellent mechanical properties, and delivers growth factors to the wound. Specifically, the presence of Ca 2+ in the silk protein leads to a solid adhesion to the wet tissue through a chelation reaction with water-trapping behavior; the integrated chitosan fabric and CaCO 3 particles ensure enhanced mechanical strength of the silk gel for better adhesion and robustness during wound repair; and the preloaded growth factor further promoted wound healing. The results showed the adhesion and tensile breaking strength were as high as 93.79 kPa and 47.20 kPa, respectively. MSCCA@CaCO 3 -aFGF could remedy the wound model in 13 days, with 99.41 % wound shrinkage without severe inflammatory responses. Due to strong adhesion properties and mechanical strength, MSCCA@CaCO 3 -aFGF can be a promising alternative to conventional sutures and tissue closure staples for wound closure and healing. Therefore, MSCCA@CaCO 3 -aFGF is expected to be a strong candidate for the next generation of adhesives.
研究领域:
生物医学工程
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材料科学
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组织工程
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伤口愈合
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）